(Circular) (Six-base) MAPSORT of: pFN21AE0692.seq Check: 2381 from: 1 to: 5911 pFN21AE0692 5911 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2285 3721 278 Size: 53 83 186 936 749 1436 2468 Fragments arranged by size: 2468 1436 936 749 186 83 53 AccI GT'mk_AC Cuts at: 1937 2601 3254 1937 Size: 664 653 4594 Fragments arranged by size: 4594 664 653 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2679 3150 3974 5358 5626 1632 Size: 1047 471 824 1384 268 1917 Fragments arranged by size: 1917 1384 1047 824 471 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 4810 5787 1203 Size: 3607 977 1327 Fragments arranged by size: 3607 1327 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5911 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AlwNI CAG_nnn'CTG Cuts at: 3654 5226 3654 Size: 1572 4339 ApaLI G'TGCA_C Cuts at: 2452 3968 5124 2452 Size: 1516 1156 3239 Fragments arranged by size: 3239 1516 1156 ApoI r'AATT_y Cuts at: 1493 2888 2982 3220 3446 3598 5707 1493 Size: 1395 94 238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1395 238 226 152 94 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 3236 1870 Size: 73 1293 4545 Fragments arranged by size: 4545 1293 73 BamHI G'GATC_C Cuts at: 3241 3608 3241 Size: 367 5544 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2467 2621 3232 4687 Size: 325 217 627 279 401 154 611 1455 Cuts at: 4687 618 Size: 1842 Fragments arranged by size: 1842 1455 627 611 401 325 279 217 154 BanII G_rGCy'C Cuts at: 1391 3230 1391 Size: 1839 4072 BbeI G_GCGC'C Cuts at: 2070 2070 Size: 5911 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2211 2970 928 Size: 971 312 759 3869 Fragments arranged by size: 3869 971 759 312 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4102 4136 1903 Size: 34 2165 34 3678 Fragments arranged by size: 3678 2165 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 1548 Size: 5911 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4522 136 Size: 107 122 71 631 605 2850 1525 Fragments arranged by size: 2850 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2698 3114 5906 2698 Size: 416 2792 2703 Fragments arranged by size: 2792 2703 416 BlpI GC'TnA_GC Cuts at: 2403 3328 2403 Size: 925 4986 Bme1580I G_kGCm'C Cuts at: 1790 2456 3972 5128 1790 Size: 666 1516 1156 2573 Fragments arranged by size: 2573 1516 1156 666 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3642 4600 442 Size: 416 53 83 399 24 2225 958 1753 Fragments arranged by size: 2225 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2236 2253 4571 1239 Size: 863 134 17 2318 2579 Fragments arranged by size: 2579 2318 863 134 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4036 4901 5199 4036 Size: 865 298 4748 Fragments arranged by size: 4748 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2600 4574 882 Size: 617 1101 1974 2219 Fragments arranged by size: 2219 1974 1101 617 BsaAI yAC'GTr Cuts at: 493 1204 3702 493 Size: 711 2498 2702 Fragments arranged by size: 2702 2498 711 BsaBI GATnn'nnATC Cuts at: 2438 5905 2438 Size: 3467 2444 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2282 3718 4100 Size: 53 83 186 936 534 215 1436 382 Cuts at: 4100 4735 275 Size: 635 1451 Fragments arranged by size: 1451 1436 936 635 534 382 215 186 83 53 BsaWI w'CCGG_w Cuts at: 4344 4742 5016 5163 5574 4344 Size: 398 274 147 411 4681 Fragments arranged by size: 4681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2155 2185 2504 2534 1178 Size: 30 165 30 752 30 319 30 4555 Fragments arranged by size: 4555 752 319 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5911 BseYI C'CCAG_C Cuts at: 2246 2472 5114 5610 2246 Size: 226 2642 496 2547 Fragments arranged by size: 2642 2547 496 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3361 4122 5150 1540 Size: 275 186 1360 761 1028 2301 Fragments arranged by size: 2301 1360 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2456 3972 4057 5128 2456 Size: 1516 85 1071 3239 Fragments arranged by size: 3239 1516 1071 85 BsmI GAATG_Cn' Cuts at: 3422 3515 3422 Size: 93 5818 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2456 3230 3972 4057 5128 1391 Size: 399 666 774 742 85 1071 2174 Fragments arranged by size: 2174 1071 774 742 666 399 85 BspEI T'CCGG_A Cuts at: 5574 5574 Size: 5911 BspHI T'CATG_A Cuts at: 2335 2536 3800 5530 2335 Size: 201 1264 1730 2716 Fragments arranged by size: 2716 1730 1264 201 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3266 844 Size: 2422 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3798 1394 Size: 429 1975 3507 Fragments arranged by size: 3507 1975 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2571 4406 4580 65 Size: 1183 1323 1835 174 1396 Fragments arranged by size: 1835 1396 1323 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4555 4742 1700 Size: 110 2745 187 2869 Fragments arranged by size: 2869 2745 187 110 BsrGI T'GTAC_A Cuts at: 96 2883 96 Size: 2787 3124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5911 BssSI C'ACGA_G Cuts at: 3971 4983 3971 Size: 1012 4899 BstAPI GCAn_nnn'nTGC Cuts at: 794 2413 794 Size: 1619 4292 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5911 BstYI r'GATC_y Cuts at: 1972 2698 2809 2837 3114 3241 3608 3993 4010 Size: 726 111 28 277 127 367 385 17 Cuts at: 4010 5462 5548 5560 5906 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 726 385 367 346 277 127 111 86 28 17 12 Bsu36I CC'TnA_GG Cuts at: 2715 2750 2715 Size: 35 5876 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5359 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 2668 3427 4221 4241 2668 Size: 759 794 20 4338 Fragments arranged by size: 4338 794 759 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 3215 3215 Size: 5911 DraIII CAC_nnn'GTG Cuts at: 2230 2230 Size: 5911 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3358 4247 5876 Size: 54 1425 50 275 18 1528 889 1629 Cuts at: 5876 8 Size: 43 Fragments arranged by size: 1629 1528 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3358 1537 Size: 275 1546 4090 Fragments arranged by size: 4090 1546 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3841 1353 Size: 2488 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4498 4872 5018 387 Size: 1064 138 2909 374 146 1280 Fragments arranged by size: 2909 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2236 2253 2679 3150 3974 4571 Size: 393 470 134 17 426 471 824 597 Cuts at: 4571 5358 5626 1239 Size: 787 268 1524 Fragments arranged by size: 1524 824 787 597 471 470 426 393 268 134 17 EcoNI CCTnn'n_nnAGG Cuts at: 2268 2268 Size: 5911 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FspI TGC'GCA Cuts at: 4417 4417 Size: 5911 HaeII r_GCGC'y Cuts at: 1678 2070 5058 1678 Size: 392 2988 2531 Fragments arranged by size: 2988 2531 392 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4552 4584 4626 4658 1373 Size: 32 135 32 2980 32 42 32 2626 Fragments arranged by size: 2980 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3501 669 Size: 988 281 1317 246 3079 Fragments arranged by size: 3079 1317 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KasI G'GCGC_C Cuts at: 2066 2066 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5025 5209 1305 Size: 3720 184 2007 Fragments arranged by size: 3720 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4089 Fragments arranged by size: 4089 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 2387 3869 4228 4387 518 Size: 648 1147 74 1482 359 159 2042 Fragments arranged by size: 2042 1482 1147 648 359 159 74 MspA1I CmG'CkG Cuts at: 1606 2476 3327 4004 5152 5397 1606 Size: 870 851 677 1148 245 2120 Fragments arranged by size: 2120 1148 870 851 677 245 NarI GG'CG_CC Cuts at: 2067 2067 Size: 5911 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5359 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 3358 3358 Size: 5911 NspI r_CATG'y Cuts at: 1132 1229 3269 4814 1132 Size: 97 2040 1545 2229 Fragments arranged by size: 2229 2040 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5911 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 2789 1108 Size: 1681 4230 PfoI T'CCnGG_A Cuts at: 2269 2269 Size: 5911 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5911 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PstI C_TGCA'G Cuts at: 830 3263 830 Size: 2433 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 2515 3259 4394 5075 5266 826 Size: 219 1470 744 1135 681 191 1471 Fragments arranged by size: 1471 1470 1135 744 681 219 191 SfoI GGC'GCC Cuts at: 2068 2068 Size: 5911 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 3238 1872 Size: 1366 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4015 4916 5178 820 Size: 197 926 2072 901 262 1553 Fragments arranged by size: 2072 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 3180 1517 Size: 1663 4248 StyI C'CwwG_G Cuts at: 513 1065 2052 3350 513 Size: 552 987 1298 3074 Fragments arranged by size: 3074 1298 987 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3063 3938 3955 4108 4293 4738 325 Size: 1255 1483 875 17 153 185 445 1498 Fragments arranged by size: 1498 1483 1255 875 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2883 3196 4157 Size: 274 80 33 51 1443 906 313 961 Cuts at: 4157 5869 96 Size: 1712 138 Fragments arranged by size: 1712 1443 961 906 313 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2155 2187 5871 5903 2155 Size: 32 3684 32 2163 Fragments arranged by size: 3684 2163 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2597 1316 Size: 1281 4630 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2059 2059 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 3236 1870 Size: 1366 4545 XmnI GAAnn'nnTTC Cuts at: 4040 4040 Size: 5911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2283 3719 276 Size: 53 83 186 936 749 1436 2468 Fragments arranged by size: 2468 1436 936 749 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI