################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AlwNI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI HincII HindII HpaI KpnI MamI MfeI MluI MroI MseI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcyI 1618 GGCGCC G[GA]^CG[CT]C AocI 244 CCTGAGG CC^T[ACGT]AGG AspI 232 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1575 CCCGGG C^[CT]CG[GA]G AviII 1316 TGCGCA TGC^GCA BbeI 1618 GGCGCC GGCGC^C BbrPI 1828 CACGTG CAC^GTG BfaI 202 CTAG C^TAG BsaAI 1828 CACGTG [CT]AC^GT[GA] BsaHI 1618 GGCGCC G[GA]^CG[CT]C BsaI 732 GAGACC GGTCTC(1/5) BsiEI 1437 CGGCCG CG[GA][CT]^CG BsmI 29 GCATTC GAATGC(1/-1) BsoBI 1575 CCCGGG C^[CT]CG[GA]G BsrDI 305 GCAATG GCAATG(2/0) Bsu36I 244 CCTGAGG CC^T[ACGT]AGG EagI 1437 CGGCCG C^GGCCG EclXI 1437 CGGCCG C^GGCCG Eco52I 1437 CGGCCG C^GGCCG Eco81I 244 CCTGAGG CC^T[ACGT]AGG FseI 1797 GGCCGGCC GGCCGG^CC FspI 1316 TGCGCA TGC^GCA HgaI 1433 GACGC GACGC(5/10) Hin1I 1618 GGCGCC G[GA]^CG[CT]C HindIII 181 AAGCTT A^AGCTT KasI 1618 GGCGCC G^GCGCC KspI 1138 CCGCGG CCGC^GG MaeI 202 CTAG C^TAG MaeIII 101 GTGAC ^GT[ACGT]AC NarI 1618 GGCGCC GG^CGCC NcoI 821 CCATGG C^CATGG NotI 1436 GCGGCCGC GC^GGCCGC PflFI 232 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 625 GACTC GAGTC(4/5) PmaCI 1828 CACGTG CAC^GTG PmlI 1828 CACGTG CAC^GTG PstI 162 CTGCAG CTGCA^G SacI 19 GAGCTC GAGCT^C SacII 1138 CCGCGG CCGC^GG ScaI 170 AGTACT AGT^ACT SfcI 162 CTGCAG C^T[GA][CT]AG SfiI 1792 GGCCTGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1618 GGCGCC GGC^GCC SmaI 1575 CCCGGG CCC^GGG SmlI 1303 CTCAAG C^T[CT][GA]AG Tsp45I 101 GTGAC ^GT[GC]AC Tth111I 232 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1592 CCAAGCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1575 CCCGGG C^CCGGG XmaI 1575 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 171 GTAC GT^AC 705 GTAC Alw44I 1179 GTGCAC G^TGCAC 1288 GTGCAC ApaI 1480 GGGCCC GGGCC^C 1578 GGGCCC ApaLI 1179 GTGCAC G^TGCAC 1288 GTGCAC Asp700I 180 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1668 GAAGCTGTTC BcgI 987 CGACTACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 126 GCAGGAGTATCG BspLU11I 935 ACATGT A^CATGT 1175 ACATGT BspMI 947 ACCTGC ACCTGC(4/8) 1385 ACCTGC BssHII 1680 GCGCGC G^CGCGC 1682 GCGCGC BstAPI 1181 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1265 GCATCCACTGC BstYI 344 AGATCC [GA]^GATC[CT] 840 GGATCT DsaI 821 CCATGG C^C[GA][CT]GG 1138 CCGCGG MflI 344 AGATCC [GA]^GATC[CT] 840 GGATCT RsaI 171 GTAC GT^AC 705 GTAC SapI 1060 GAAGAGC GCTCTTC(1/4) 1473 GCTCTTC TfiI 682 GAATC G^A[AT]TC 855 GAATC XhoII 344 AGATCC [GA]^GATC[CT] 840 GGATCT XmnI 180 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1668 GAAGCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AflIII AluI AlwI AspHI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI StuI StyI TaqI TseI TspRI TthHB8I