################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccIII AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII Hin1I HpaI HphI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Tru9I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 741 GTCTAC GT^[AC][GT]AC AccII 8 CGCG CG^CG AclI 1087 AACGTT AA^CGTT Alw44I 1127 GTGCAC G^TGCAC ApaLI 1127 GTGCAC G^TGCAC AspHI 1127 GTGCAC G[AT]GC[AT]^C AspI 1001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1245 TGGCCA TGG^CCA BanII 556 GAGCCC G[GA]GC[CT]^C BbsI 320 GAAGAC GAAGAC(2/6) BlpI 1159 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1159 GCTCAGC GC^T[ACGT]AGC BpuAI 320 GAAGAC GAAGAC(2/6) BseRI 79 GAGGAG GAGGAG(10/8) BsgI 719 CTGCAC GTGCAG(16/14) BsiHKAI 1127 GTGCAC G[AT]GC[AT]^C BspDI 948 ATCGAT AT^CGAT BsrFI 468 GCCGGC [GA]^CCGG[CT] BstAPI 922 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 317 TTCGAA TT^CGAA BstUI 8 CGCG CG^CG CelII 1159 GCTCAGC GC^T[ACGT]AGC Cfr10I 468 GCCGGC [GA]^CCGG[CT] ClaI 948 ATCGAT AT^CGAT EaeI 1245 TGGCCA [CT]^GGCC[GA] EarI 436 GAAGAG CTCTTC(1/4) EcoNI 1231 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 173 GAATTC G^AATTC EcoT14I 1101 CCAAGG C^C[AT][AT]GG HapII 469 CCGG C^CGG HgaI 6 GACGC GACGC(5/10) HincII 217 GTCAAC GT[CT]^[GA]AC HindII 217 GTCAAC GT[CT]^[GA]AC HindIII 67 AAGCTT A^AGCTT HpaII 469 CCGG C^CGG Ksp632I 436 GAAGAG CTCTTC(1/4) MaeII 1088 ACGT A^CGT MluNI 1245 TGGCCA TGG^CCA MscI 1245 TGGCCA TGG^CCA MspI 469 CCGG C^CGG MvnI 8 CGCG CG^CG NaeI 468 GCCGGC GCC^GGC NgoMIV 468 GCCGGC G^CCGGC NspV 317 TTCGAA TT^CGAA PflFI 1001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 1087 AACGTT AA^CGTT SfuI 317 TTCGAA TT^CGAA SspI 139 AATATT AAT^ATT StyI 1101 CCAAGG C^C[AT][AT]GG TfiI 1191 GAATC G^A[AT]TC Tsp45I 604 GTGAC ^GT[GC]AC Tth111I 1001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 762 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AciI 1153 CCGC CCGC(-3/-1) 553 GCGG AcsI 173 GAATTC [GA]^AATT[CT] 346 GAATTT AlwI 567 GGATC GGATC(4/5) 759 GGATC ApoI 173 GAATTC [GA]^AATT[CT] 346 GAATTT BanI 597 GGCACC G^G[CT][GA]CC 624 GGTGCC BcgI 387 CGATAAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 950 CGATGGGCGTGC BfaI 223 CTAG C^TAG 763 CTAG BmyI 556 GAGCCC G[AGT]GC[ACT]^C 1127 GTGCAC BpmI 1254 CTCCAG CTGGAG(16/14) 517 CTGGAG BsaJI 203 CCAGGG C^C[ACGT][ACGT]GG 1101 CCAAGG Bsp1286I 556 GAGCCC G[AGT]GC[ACT]^C 1127 GTGCAC BspMI 681 ACCTGC ACCTGC(4/8) 1237 GCAGGT BstF5I 57 CATCC GGATG(2/0) 1270 GGATG BstYI 567 GGATCT [GA]^GATC[CT] 759 GGATCT CfoI 9 GCGC GCG^C 98 GCGC DraII 117 AGGTCCT [GA]G^G[ACGT]CC[CT] 891 GGGACCC EcoO109I 117 AGGTCCT [GA]G^G[ACGT]CC[CT] 891 GGGACCC FokI 57 CATCC GGATG(9/13) 1270 GGATG HhaI 9 GCGC GCG^C 98 GCGC HinP1I 9 GCGC G^CGC 98 GCGC MaeI 223 CTAG C^TAG 763 CTAG MaeIII 604 GTGAC ^GT[ACGT]AC 748 GTAAC MflI 567 GGATCT [GA]^GATC[CT] 759 GGATCT NspI 254 GCATGC [GA]CATG^[CT] 1025 GCATGC PpuMI 117 AGGTCCT [GA]G^G[AT]CC[CT] 891 GGGACCC ScaI 92 AGTACT AGT^ACT 871 AGTACT SfaNI 1071 GATGC GCATC(5/9) 158 GCATC SphI 254 GCATGC GCATG^C 1025 GCATGC Tsp509I 174 AATT ^AATT 347 AATT XcmI 1037 CCAATGGGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1134 CCAGAGTAAGTTTGG XhoII 567 GGATCT [GA]^GATC[CT] 759 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AfaI AluI AlwNI AvaII BbvI BsiYI BslI BsmFI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HinfI ItaI MboI MboII MnlI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI TaqI TseI TspRI TthHB8I