(Circular) (Six-base) MAPSORT of: pFN21AB7644.seq Check: 409 from: 1 to: 5875 pFN21AB7644 5875 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2346 2346 Size: 5875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2229 2331 3218 1937 Size: 292 102 887 4594 Fragments arranged by size: 4594 887 292 102 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 2669 3938 5322 5590 1632 Size: 521 516 1269 1384 268 1917 Fragments arranged by size: 1917 1384 1269 521 516 268 AfeI AGC'GCT Cuts at: 2255 2255 Size: 5875 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2364 1184 Size: 1180 4695 AlwNI CAG_nnn'CTG Cuts at: 2151 2163 2468 3058 3618 5190 2151 Size: 12 305 590 560 1572 2836 Fragments arranged by size: 2836 1572 590 560 305 12 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 2390 2790 3184 3410 3562 5671 1493 Size: 897 400 394 226 152 2109 1697 Fragments arranged by size: 2109 1697 897 400 394 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 3200 1870 Size: 73 1257 4545 Fragments arranged by size: 4545 1257 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2667 3196 4651 618 Size: 325 217 627 880 529 1455 1842 Fragments arranged by size: 1842 1455 880 627 529 325 217 BanII G_rGCy'C Cuts at: 1391 2052 2738 2924 3194 1391 Size: 661 686 186 270 4072 Fragments arranged by size: 4072 686 661 270 186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2436 928 Size: 971 537 4367 Fragments arranged by size: 4367 971 537 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2300 3767 4977 2300 Size: 1467 1210 3198 Fragments arranged by size: 3198 1467 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BfrBI ATG'CAT Cuts at: 2528 2528 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2554 4486 136 Size: 107 122 71 631 605 882 1932 1525 Fragments arranged by size: 1932 1525 882 631 605 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 2475 2771 3292 2475 Size: 296 521 5058 Fragments arranged by size: 5058 521 296 Bme1580I G_kGCm'C Cuts at: 1790 3936 5092 1790 Size: 2146 1156 2573 Fragments arranged by size: 2573 2146 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2083 2932 3606 Size: 416 53 83 399 24 666 849 674 Cuts at: 3606 4564 442 Size: 958 1753 Fragments arranged by size: 1753 958 849 674 666 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2197 2490 2531 4535 1239 Size: 958 293 41 2004 2579 Fragments arranged by size: 2579 2004 958 293 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2153 2929 4000 4865 5163 2153 Size: 776 1071 865 298 2865 Fragments arranged by size: 2865 1071 865 776 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2560 3666 493 Size: 711 1356 1106 2702 Fragments arranged by size: 2702 1356 1106 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2522 2552 2558 2588 1178 Size: 30 165 30 1119 30 6 30 4465 Fragments arranged by size: 4465 1119 165 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2716 1186 Size: 1530 4345 BseYI C'CCAG_C Cuts at: 2219 2673 3071 5078 5574 2219 Size: 454 398 2007 496 2520 Fragments arranged by size: 2520 2007 496 454 398 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2196 2324 1163 Size: 573 460 128 4714 Fragments arranged by size: 4714 573 460 128 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2738 3936 4021 5092 2738 Size: 1198 85 1071 3521 Fragments arranged by size: 3521 1198 1071 85 BsmI GAATG_Cn' Cuts at: 2530 3386 3479 2530 Size: 856 93 4926 Fragments arranged by size: 4926 856 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2738 2924 3194 3936 4021 5092 Size: 399 262 686 186 270 742 85 1071 Cuts at: 5092 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 686 399 270 262 186 85 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 3764 5494 3764 Size: 1730 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 3230 844 Size: 1502 884 3489 Fragments arranged by size: 3489 1502 884 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3024 4370 4544 65 Size: 1183 1776 1346 174 1396 Fragments arranged by size: 1776 1396 1346 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2367 4519 4706 1700 Size: 110 557 2152 187 2869 Fragments arranged by size: 2869 2152 557 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 2268 2277 2361 794 Size: 1474 9 84 4308 Fragments arranged by size: 4308 1474 84 9 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstXI CCAn_nnnn'nTGG Cuts at: 2096 2509 3144 2096 Size: 413 635 4827 Fragments arranged by size: 4827 635 413 BstYI r'GATC_y Cuts at: 1972 3205 3572 3957 3974 5426 5512 5524 5870 Size: 1233 367 385 17 1452 86 12 346 Cuts at: 5870 1972 Size: 1977 Fragments arranged by size: 1977 1452 1233 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2028 2379 2877 3066 513 Size: 552 963 351 498 189 3322 Fragments arranged by size: 3322 963 552 498 351 189 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 2341 3112 3391 4185 4205 2341 Size: 771 279 794 20 4011 Fragments arranged by size: 4011 794 771 279 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2286 2321 529 Size: 35 1722 35 4083 Fragments arranged by size: 4083 1722 35 35 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2600 4882 809 Size: 507 1284 2282 1802 Fragments arranged by size: 2282 1802 1284 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2376 2806 3322 Size: 54 1425 50 275 18 546 430 516 Cuts at: 3322 4211 5840 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 546 516 430 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3805 1353 Size: 2452 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2213 2865 4462 4836 4982 387 Size: 1064 138 624 652 1597 374 146 1280 Fragments arranged by size: 1597 1280 1064 652 624 374 146 138 EcoICRI GAG'CTC Cuts at: 2736 2736 Size: 5875 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2197 2490 2531 2669 3938 4535 Size: 393 521 44 293 41 138 1269 597 Cuts at: 4535 5322 5590 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1269 787 597 521 393 293 268 138 44 41 EcoNI CCTnn'n_nnAGG Cuts at: 2402 2651 2402 Size: 249 5626 EcoO109I rG'GnC_Cy Cuts at: 2137 3077 2137 Size: 940 4935 EcoRI G'AATT_C Cuts at: 2390 3184 3562 2390 Size: 794 378 4703 Fragments arranged by size: 4703 794 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2260 2260 Size: 5875 FspI TGC'GCA Cuts at: 3030 4381 3030 Size: 1351 4524 HaeII r_GCGC'y Cuts at: 1678 2257 5022 1678 Size: 579 2765 2531 Fragments arranged by size: 2765 2531 579 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2558 2590 4516 4548 4590 Size: 32 135 32 986 32 1926 32 42 Cuts at: 4590 4622 1373 Size: 32 2626 Fragments arranged by size: 2626 1926 986 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3152 4989 5173 1305 Size: 1847 1837 184 2007 Fragments arranged by size: 2007 1847 1837 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 2808 10 Size: 54 1425 343 546 430 3077 Fragments arranged by size: 3077 1425 546 430 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3833 4192 4351 518 Size: 648 2667 359 159 2042 Fragments arranged by size: 2667 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2194 2374 2509 2572 2673 3291 3968 Size: 424 164 180 135 63 101 618 677 Cuts at: 3968 5116 5361 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 618 424 245 180 164 135 101 63 NcoI C'CATG_G Cuts at: 513 1065 2877 3066 513 Size: 552 1812 189 3322 Fragments arranged by size: 3322 1812 552 189 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NsiI A_TGCA'T Cuts at: 2530 2530 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 3035 3233 4778 1132 Size: 97 1806 198 1545 2229 Fragments arranged by size: 2229 1806 1545 198 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PfoI T'CCnGG_A Cuts at: 2052 2115 2139 2052 Size: 63 24 5788 Fragments arranged by size: 5788 63 24 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PmlI CAC'GTG Cuts at: 2560 2560 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2444 2476 3944 3976 5457 5489 2444 Size: 32 1468 32 1481 32 2830 Fragments arranged by size: 2830 1481 1468 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 3077 2137 Size: 940 4935 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PstI C_TGCA'G Cuts at: 830 2743 3227 830 Size: 1913 484 3478 Fragments arranged by size: 3478 1913 484 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 2194 2374 2509 2572 2194 Size: 180 135 63 5497 Fragments arranged by size: 5497 180 135 63 SacI G_AGCT'C Cuts at: 2738 2738 Size: 5875 SacII CC_GC'GG Cuts at: 2031 2031 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SanDI GG'GwC_CC Cuts at: 3077 3077 Size: 5875 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 2739 3223 4358 5039 5230 826 Size: 219 1694 484 1135 681 191 1471 Fragments arranged by size: 1694 1471 1135 681 484 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 2908 3979 4880 5142 820 Size: 197 926 225 740 1071 901 262 1553 Fragments arranged by size: 1553 1071 926 901 740 262 225 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3035 3233 1132 Size: 1903 198 3774 Fragments arranged by size: 3774 1903 198 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 2589 1517 Size: 1072 4803 StyI C'CwwG_G Cuts at: 513 1065 2877 3066 3314 513 Size: 552 1812 189 248 3074 Fragments arranged by size: 3074 1812 552 248 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3902 3919 4072 4257 4702 325 Size: 1255 2322 17 153 185 445 1498 Fragments arranged by size: 2322 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4121 5833 96 Size: 274 80 33 51 1443 2144 1712 138 Fragments arranged by size: 2144 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5835 5867 5835 Size: 32 5843 Tth111I GACn'n_nGTC Cuts at: 1316 2327 1316 Size: 1011 4864 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI