(Circular) (Six-base) MAPSORT of: pFN21AB7607.seq Check: 8608 from: 1 to: 5602 pFN21AB7607 5602 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2202 3412 278 Size: 53 83 186 936 666 1210 2468 Fragments arranged by size: 2468 1210 936 666 186 83 53 AccI GT'mk_AC Cuts at: 1937 2945 1937 Size: 1008 4594 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2246 3665 5049 5317 1632 Size: 614 1419 1384 268 1917 Fragments arranged by size: 1917 1419 1384 614 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 4501 5478 1203 Size: 3298 977 1327 Fragments arranged by size: 3298 1327 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 2393 2471 1166 Size: 1227 78 4297 Fragments arranged by size: 4297 1227 78 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AlwNI CAG_nnn'CTG Cuts at: 3345 4917 3345 Size: 1572 4030 ApaI G_GGCC'C Cuts at: 2031 2699 2031 Size: 668 4934 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2807 2834 2911 3137 3289 5398 1493 Size: 1314 27 77 226 152 2109 1697 Fragments arranged by size: 2109 1697 1314 226 152 77 27 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2927 1870 Size: 73 80 904 4545 Fragments arranged by size: 4545 904 80 73 BamHI G'GATC_C Cuts at: 2932 3299 2932 Size: 367 5235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2680 2780 2825 2923 4378 Size: 325 217 627 893 100 45 98 1455 Cuts at: 4378 618 Size: 1842 Fragments arranged by size: 1842 1455 893 627 325 217 100 98 45 BanII G_rGCy'C Cuts at: 1391 2031 2498 2642 2699 2921 1391 Size: 640 467 144 57 222 4072 Fragments arranged by size: 4072 640 467 222 144 57 BbeI G_GCGC'C Cuts at: 2784 2784 Size: 5602 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3793 3827 1903 Size: 34 1856 34 3678 Fragments arranged by size: 3678 1856 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3494 4704 3494 Size: 1210 4392 BclI T'GATC_A Cuts at: 1548 1548 Size: 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2454 4213 Size: 107 122 71 631 605 337 445 1759 Cuts at: 4213 136 Size: 1525 Fragments arranged by size: 1759 1525 631 605 445 337 122 107 71 BglII A'GATC_T Cuts at: 2895 5597 2895 Size: 2702 2900 BlpI GC'TnA_GC Cuts at: 3019 3019 Size: 5602 Bme1580I G_kGCm'C Cuts at: 1790 2031 2097 2372 2685 2699 2828 3663 4819 Size: 241 66 275 313 14 129 835 1156 Cuts at: 4819 1790 Size: 2573 Fragments arranged by size: 2573 1156 835 313 275 241 129 66 14 BmgBI CAC'GTC Cuts at: 1318 2373 1318 Size: 1055 4547 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2061 2182 2740 Size: 416 53 83 399 24 644 121 558 Cuts at: 2740 3333 4291 442 Size: 593 958 1753 Fragments arranged by size: 1753 958 644 593 558 416 399 121 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 2186 2512 4262 1239 Size: 938 9 326 1750 2579 Fragments arranged by size: 2579 1750 938 326 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2637 4265 882 Size: 617 1138 1628 2219 Fragments arranged by size: 2219 1628 1138 617 BsaAI yAC'GTr Cuts at: 493 1204 3393 493 Size: 711 2189 2702 Fragments arranged by size: 2702 2189 711 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2199 2781 3409 3791 Size: 53 83 186 936 666 582 628 382 Cuts at: 3791 4426 275 Size: 635 1451 Fragments arranged by size: 1451 936 666 635 628 582 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2271 4035 4433 4707 4854 5265 2271 Size: 1764 398 274 147 411 2608 Fragments arranged by size: 2608 1764 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2159 2189 1178 Size: 30 165 30 756 30 4591 Fragments arranged by size: 4591 756 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2228 1186 Size: 988 54 4560 Fragments arranged by size: 4560 988 54 BseYI C'CCAG_C Cuts at: 2497 2534 2685 4805 5301 2497 Size: 37 151 2120 496 2798 Fragments arranged by size: 2798 2120 496 151 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5029 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 3813 4841 1540 Size: 275 186 1051 761 1028 2301 Fragments arranged by size: 2301 1051 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2642 3663 3748 4819 2642 Size: 1021 85 1071 3425 Fragments arranged by size: 3425 1071 1021 85 BsmI GAATG_Cn' Cuts at: 3113 3206 3113 Size: 93 5509 BsmBI CGTCTCn'nnnn_ Cuts at: 2562 2562 Size: 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2097 2372 2498 2642 2685 2699 Size: 399 241 66 275 126 144 43 14 Cuts at: 2699 2828 2921 3663 3748 4819 1391 Size: 129 93 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 275 241 144 129 126 93 85 66 43 14 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 3491 5221 3491 Size: 1730 3872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 844 Size: 2113 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3489 1394 Size: 429 1666 3507 Fragments arranged by size: 3507 1666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4097 4271 65 Size: 1183 2849 174 1396 Fragments arranged by size: 2849 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 2177 794 Size: 1383 4219 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstXI CCAn_nnnn'nTGG Cuts at: 2473 2777 2473 Size: 304 5298 BstYI r'GATC_y Cuts at: 1972 2074 2895 2932 3299 3684 3701 5153 5239 Size: 102 821 37 367 385 17 1452 86 Cuts at: 5239 5251 5597 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 821 385 367 346 102 86 37 17 12 Bsu36I CC'TnA_GG Cuts at: 2452 2520 2548 2452 Size: 68 28 5506 Fragments arranged by size: 5506 68 28 BtgI C'CryG_G Cuts at: 513 1065 2466 2676 2727 2793 2820 2850 513 Size: 552 1401 210 51 66 27 30 3265 Fragments arranged by size: 3265 1401 552 210 66 51 30 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4863 Fragments arranged by size: 4863 683 56 BtsI GCAGTG_nn' Cuts at: 2478 3118 3912 3932 2478 Size: 640 794 20 4148 Fragments arranged by size: 4148 794 640 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2475 3049 3938 Size: 54 1425 50 275 18 645 574 889 Cuts at: 3938 5567 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 645 574 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2617 3532 1353 Size: 1264 915 3423 Fragments arranged by size: 3423 1264 915 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2557 2611 4189 4563 4709 387 Size: 1064 138 968 54 1578 374 146 1280 Fragments arranged by size: 1578 1280 1064 968 374 146 138 54 EcoICRI GAG'CTC Cuts at: 2640 2640 Size: 5602 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2186 2246 2512 3665 4262 5049 Size: 393 545 9 60 266 1153 597 787 Cuts at: 5049 5317 1239 Size: 268 1524 Fragments arranged by size: 1524 1153 787 597 545 393 268 266 60 9 EcoO109I rG'GnC_Cy Cuts at: 2027 2028 2695 2732 2815 2027 Size: 1 667 37 83 4814 Fragments arranged by size: 4814 667 83 37 1 EcoRI G'AATT_C Cuts at: 2807 2911 3289 2807 Size: 104 378 5120 Fragments arranged by size: 5120 378 104 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 2109 2784 4749 1678 Size: 431 675 1965 2531 Fragments arranged by size: 2531 1965 675 431 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4243 4275 4317 4349 1373 Size: 32 135 32 2671 32 42 32 2626 Fragments arranged by size: 2671 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2044 2946 3192 669 Size: 988 281 106 902 246 3079 Fragments arranged by size: 3079 988 902 281 246 106 HindIII A'AGCT_T Cuts at: 748 748 Size: 5602 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KasI G'GCGC_C Cuts at: 2780 2780 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4716 4900 1305 Size: 3411 184 2007 Fragments arranged by size: 3411 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2477 10 Size: 54 1425 343 645 3135 Fragments arranged by size: 3135 1425 645 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2393 2471 3560 3919 4078 518 Size: 648 1227 78 1089 359 159 2042 Fragments arranged by size: 2042 1227 1089 648 359 159 78 MspA1I CmG'CkG Cuts at: 1606 2122 2530 2652 3018 3695 4843 5088 1606 Size: 516 408 122 366 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 516 408 366 245 122 NarI GG'CG_CC Cuts at: 2781 2781 Size: 5602 NcoI C'CATG_G Cuts at: 513 1065 2676 2850 513 Size: 552 1611 174 3265 Fragments arranged by size: 3265 1611 552 174 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2960 4505 1132 Size: 97 1731 1545 2229 Fragments arranged by size: 2229 1731 1545 97 PasI CC'CwG_GG Cuts at: 1348 2327 1348 Size: 979 4623 PciI A'CATG_T Cuts at: 4501 4501 Size: 5602 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5602 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3703 5184 5216 3671 Size: 32 1481 32 4057 Fragments arranged by size: 4057 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2732 2815 2732 Size: 83 5519 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PspOMI G'GGCC_C Cuts at: 2027 2695 2027 Size: 668 4934 PstI C_TGCA'G Cuts at: 830 2954 830 Size: 2124 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 SacI G_AGCT'C Cuts at: 2642 2642 Size: 5602 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SapI GCTCTTCn'nnn_ Cuts at: 2617 2617 Size: 5602 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 3850 5562 3850 Size: 1712 3890 SfcI C'TryA_G Cuts at: 826 1045 2428 2785 2950 4085 4766 4957 826 Size: 219 1383 357 165 1135 681 191 1471 Fragments arranged by size: 1471 1383 1135 681 357 219 191 165 SfoI GGC'GCC Cuts at: 2782 2782 Size: 5602 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2025 2929 1872 Size: 153 904 4545 Fragments arranged by size: 4545 904 153 SmlI C'TyrA_G Cuts at: 820 1017 1943 3706 4607 4869 820 Size: 197 926 1763 901 262 1553 Fragments arranged by size: 1763 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2049 2340 2676 2850 3041 513 Size: 552 984 291 336 174 191 3074 Fragments arranged by size: 3074 984 552 336 291 191 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3629 3646 3799 3984 4429 325 Size: 1255 2049 17 153 185 445 1498 Fragments arranged by size: 2049 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3848 5560 96 Size: 274 80 33 51 1443 1871 1712 138 Fragments arranged by size: 1871 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2830 2862 5562 5594 2830 Size: 32 2700 32 2838 Fragments arranged by size: 2838 2700 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2857 2857 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2023 2927 1870 Size: 153 904 4545 Fragments arranged by size: 4545 904 153 XmnI GAAnn'nnTTC Cuts at: 3731 3731 Size: 5602 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2200 3410 276 Size: 53 83 186 936 666 1210 2468 Fragments arranged by size: 2468 1210 936 666 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI