Restriction map for
pFN21AB7765
##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiHKAI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FspI HgaI
HindIII HpaI KpnI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 538 GTCGAC GT^[AC][GT]AC
AlwI 48 GGATC GGATC(4/5)
Asp700I 925 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 496 GGGCTC G[GA]GC[CT]^C
BbsI 667 GTCTTC GAAGAC(2/6)
BbvCI 699 CCTCAGC CCTCAGC(-5/-2)
BglI 359 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 496 GGGCTC G[AGT]GC[ACT]^C
BpuAI 667 GTCTTC GAAGAC(2/6)
BsaI 1292 GAGACC GGTCTC(1/5)
BsaWI 1106 ACCGGA [AT]^CCGG[AT]
BsiWI 245 CGTACG C^GTACG
BsmBI 1147 GAGACG CGTCTC(1/5)
BsmI 763 GAATGC GAATGC(1/-1)
Bsp1286I 496 GGGCTC G[AGT]GC[ACT]^C
BspMI 440 ACCTGC ACCTGC(4/8)
BsrDI 68 GCAATG GCAATG(2/0)
BstAPI 549 GCACCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1191 CCACGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI 1081 GAATTC G^AATTC
EcoT14I 1116 CCATGG C^C[AT][AT]GG
FseI 363 GGCCGGCC GGCCGG^CC
HincII 538 GTCGAC GT[CT]^[GA]AC
HindII 538 GTCGAC GT[CT]^[GA]AC
MaeII 709 ACGT A^CGT
NcoI 1116 CCATGG C^CATGG
PflMI 192 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1294 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1234 CTGCAG CTGCA^G
SalI 538 GTCGAC G^TCGAC
SapI 800 GAAGAGC GCTCTTC(1/4)
SspI 995 AATATT AAT^ATT
StuI 302 AGGCCT AGG^CCT
StyI 1116 CCATGG C^C[AT][AT]GG
Van91I 192 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 925 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 970 GAATTT [GA]^AATT[CT]
1081 GAATTC
AcyI 279 GGCGCC G[GA]^CG[CT]C
356 GGCGCC
ApoI 970 GAATTT [GA]^AATT[CT]
1081 GAATTC
BanI 279 GGCGCC G^G[CT][GA]CC
356 GGCGCC
BbeI 279 GGCGCC GGCGC^C
356 GGCGCC
BsaHI 279 GGCGCC G[GA]^CG[CT]C
356 GGCGCC
BseRI 96 CTCCTC GAGGAG(10/8)
697 CTCCTC
DpnII 49 GATC ^GATC
565 GATC
DraII 83 GGGTCCC [GA]G^G[ACGT]CC[CT]
585 AGGTCCC
EarI 34 GAAGAG CTCTTC(1/4)
800 GAAGAG
EcoO109I 83 GGGTCCC [GA]G^G[ACGT]CC[CT]
585 AGGTCCC
Hin1I 279 GGCGCC G[GA]^CG[CT]C
356 GGCGCC
KasI 279 GGCGCC G^GCGCC
356 GGCGCC
Ksp632I 34 GAAGAG CTCTTC(1/4)
800 GAAGAG
MboI 49 GATC ^GATC
565 GATC
MseI 769 TTAA T^TAA
1000 TTAA
MslI 591 CATTTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
758 CACCTGAATG
NaeI 276 GCCGGC GCC^GGC
364 GCCGGC
NarI 279 GGCGCC GG^CGCC
356 GGCGCC
NdeII 49 GATC ^GATC
565 GATC
NgoMIV 276 GCCGGC G^CCGGC
364 GCCGGC
NspI 506 ACATGC [GA]CATG^[CT]
1049 ACATGC
PpuMI 83 GGGTCCC [GA]G^G[AT]CC[CT]
585 AGGTCCC
PvuII 196 CAGCTG CAG^CTG
1304 CAGCTG
Sau3AI 49 GATC ^GATC
565 GATC
SfcI 117 CTACAG C^T[GA][CT]AG
1234 CTGCAG
SfoI 279 GGCGCC GGC^GCC
356 GGCGCC
TaqI 539 TCGA T^CGA
811 TCGA
Tru9I 769 TTAA T^TAA
1000 TTAA
Tsp45I 724 GTGAC ^GT[GC]AC
895 GTGAC
Tsp509I 971 AATT ^AATT
1082 AATT
TthHB8I 539 TCGA T^CGA
811 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BbvI BcnI BfaI BpmI
BsaJI BsgI BsiEI BsiYI BslI
BsmAI BsmFI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DsaI
EaeI EagI EclXI Eco52I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI KspI MaeI MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PleI RsaI SacII Sau96I ScrFI
SfaNI TfiI TseI TspRI