(Circular) (Six-base) MAPSORT of: pFN21AB6154.seq Check: 4834 from: 1 to: 6631 pFN21AB6154 6631 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3633 3633 Size: 6631 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4441 278 Size: 53 83 186 936 2905 2468 Fragments arranged by size: 2905 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3974 1937 Size: 2037 4594 Acc65I G'GTAC_C Cuts at: 3952 3952 Size: 6631 AclI AA'CG_TT Cuts at: 1563 4758 5131 1563 Size: 3195 373 3063 Fragments arranged by size: 3195 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2402 2670 2786 2921 3429 3741 3945 4694 Size: 770 268 116 135 508 312 204 749 Cuts at: 4694 6078 6346 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 770 749 508 312 268 268 204 135 116 AflII C'TTAA_G Cuts at: 820 1017 3386 820 Size: 197 2369 4065 Fragments arranged by size: 4065 2369 197 AflIII A'CryG_T Cuts at: 1203 3778 5530 6507 1203 Size: 2575 1752 977 1327 Fragments arranged by size: 2575 1752 1327 977 AgeI A'CCGG_T Cuts at: 5462 5462 Size: 6631 AhdI GACnn_n'nnGTC Cuts at: 5360 5360 Size: 6631 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6631 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6631 AlwNI CAG_nnn'CTG Cuts at: 4374 5946 4374 Size: 1572 5059 ApaLI G'TGCA_C Cuts at: 4688 5844 4688 Size: 1156 5475 ApoI r'AATT_y Cuts at: 1493 3295 3940 4166 4318 6427 1493 Size: 1802 645 226 152 2109 1697 Fragments arranged by size: 2109 1802 1697 645 226 152 AseI AT'TA_AT Cuts at: 160 1032 3144 3420 5185 160 Size: 872 2112 276 1765 1606 Fragments arranged by size: 2112 1765 1606 872 276 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6631 AvaI C'yCGr_G Cuts at: 1870 1943 2251 3332 3956 1870 Size: 73 308 1081 624 4545 Fragments arranged by size: 4545 1081 624 308 73 BamHI G'GATC_C Cuts at: 2198 3961 4328 2198 Size: 1763 367 4501 Fragments arranged by size: 4501 1763 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2720 3341 3952 5407 618 Size: 325 217 627 933 621 611 1455 1842 Fragments arranged by size: 1842 1455 933 627 621 611 325 217 BanII G_rGCy'C Cuts at: 1391 2527 3370 3654 3950 1391 Size: 1136 843 284 296 4072 Fragments arranged by size: 4072 1136 843 296 284 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3912 928 Size: 971 2013 3647 Fragments arranged by size: 3647 2013 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2273 2307 3323 3357 4822 4856 1903 Size: 34 336 34 1016 34 1465 34 3678 Fragments arranged by size: 3678 1465 1016 336 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2493 3448 4523 5733 2493 Size: 955 1075 1210 3391 Fragments arranged by size: 3391 1210 1075 955 BclI T'GATC_A Cuts at: 1548 1548 Size: 6631 BfrBI ATG'CAT Cuts at: 2665 3846 2665 Size: 1181 5450 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5242 136 Size: 107 122 71 631 605 3570 1525 Fragments arranged by size: 3570 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2385 3856 6626 2385 Size: 1471 2770 2390 Fragments arranged by size: 2770 2390 1471 BlpI GC'TnA_GC Cuts at: 4048 4048 Size: 6631 Bme1580I G_kGCm'C Cuts at: 1790 2417 4692 5848 1790 Size: 627 2275 1156 2573 Fragments arranged by size: 2573 2275 1156 627 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6631 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4362 5320 442 Size: 416 53 83 399 24 2945 958 1753 Fragments arranged by size: 2945 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6631 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5291 1239 Size: 4052 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3354 3620 3824 4756 5621 5919 3354 Size: 266 204 932 865 298 4066 Fragments arranged by size: 4066 932 865 298 266 204 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2248 2999 5294 882 Size: 617 749 751 2295 2219 Fragments arranged by size: 2295 2219 751 749 617 BsaAI yAC'GTr Cuts at: 493 1204 4422 493 Size: 711 3218 2702 Fragments arranged by size: 3218 2702 711 BsaBI GATnn'nnATC Cuts at: 6625 6625 Size: 6631 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4438 4820 5455 275 Size: 53 83 186 936 2905 382 635 1451 Fragments arranged by size: 2905 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5064 5462 5736 5883 6294 5064 Size: 398 274 147 411 5401 Fragments arranged by size: 5401 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6406 Fragments arranged by size: 6406 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2517 3209 3821 1186 Size: 1331 692 612 3996 Fragments arranged by size: 3996 1331 692 612 BseYI C'CCAG_C Cuts at: 5834 6330 5834 Size: 496 6135 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6058 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4081 4842 5870 1540 Size: 275 186 2080 761 1028 2301 Fragments arranged by size: 2301 2080 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4692 4777 5848 4692 Size: 85 1071 5475 Fragments arranged by size: 5475 1071 85 BsmI GAATG_Cn' Cuts at: 2273 2749 3478 3848 4142 4235 2273 Size: 476 729 370 294 93 4669 Fragments arranged by size: 4669 729 476 370 294 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2417 2527 3370 3654 3950 4692 4777 Size: 399 627 110 843 284 296 742 85 Cuts at: 4777 5848 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 843 742 627 399 296 284 110 85 BspEI T'CCGG_A Cuts at: 6294 6294 Size: 6631 BspHI T'CATG_A Cuts at: 3115 4520 6250 3115 Size: 1405 1730 3496 Fragments arranged by size: 3496 1730 1405 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3633 3986 844 Size: 2789 353 3489 Fragments arranged by size: 3489 2789 353 BsrBI CCG'CTC Cuts at: 1394 1823 4518 1394 Size: 429 2695 3507 Fragments arranged by size: 3507 2695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3483 5126 5300 65 Size: 1183 2235 1643 174 1396 Fragments arranged by size: 2235 1643 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5275 5462 1700 Size: 110 3465 187 2869 Fragments arranged by size: 3465 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2511 96 Size: 2415 4216 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6631 BssSI C'ACGA_G Cuts at: 4691 5703 4691 Size: 1012 5619 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6631 BstBI TT'CG_AA Cuts at: 5435 5435 Size: 6631 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6631 BstXI CCAn_nnnn'nTGG Cuts at: 3163 3163 Size: 6631 BstYI r'GATC_y Cuts at: 1972 2063 2198 2385 3856 3961 4328 4713 4730 Size: 91 135 187 1471 105 367 385 17 Cuts at: 4730 6182 6268 6280 6626 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1471 1452 385 367 346 187 135 105 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6079 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5892 Fragments arranged by size: 5892 683 56 BtsI GCAGTG_nn' Cuts at: 2291 4147 4941 4961 2291 Size: 1856 794 20 3961 Fragments arranged by size: 3961 1856 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6631 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6596 DraI TTT'AAA Cuts at: 2470 3935 2470 Size: 1465 5166 DraIII CAC_nnn'GTG Cuts at: 2548 2548 Size: 6631 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5638 809 Size: 507 4322 1802 Fragments arranged by size: 4322 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 4078 4967 Size: 54 1425 50 275 18 208 2040 889 Cuts at: 4967 6596 8 Size: 1629 43 Fragments arranged by size: 2040 1629 1425 889 275 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4078 1537 Size: 275 2266 4090 Fragments arranged by size: 4090 2266 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2445 3567 4561 1353 Size: 1092 1122 994 3423 Fragments arranged by size: 3423 1122 1092 994 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5218 5592 5738 387 Size: 1064 138 3629 374 146 1280 Fragments arranged by size: 3629 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2402 2670 2786 2921 3429 3741 3945 Size: 393 770 268 116 135 508 312 204 Cuts at: 3945 4694 5291 6078 6346 1239 Size: 749 597 787 268 1524 Fragments arranged by size: 1524 787 770 749 597 508 393 312 268 268 204 135 116 EcoO109I rG'GnC_Cy Cuts at: 2248 3160 2248 Size: 912 5719 EcoRI G'AATT_C Cuts at: 3295 3940 4318 3295 Size: 645 378 5608 Fragments arranged by size: 5608 645 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2628 2926 2628 Size: 298 6333 FspI TGC'GCA Cuts at: 5137 5137 Size: 6631 HaeII r_GCGC'y Cuts at: 1678 5778 1678 Size: 4100 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2741 2773 3587 Size: 32 135 32 476 32 661 32 814 Cuts at: 3587 3619 5272 5304 5346 5378 1373 Size: 32 1653 32 42 32 2626 Fragments arranged by size: 2626 1653 814 661 476 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2213 3643 3975 4221 669 Size: 988 281 275 1430 332 246 3079 Fragments arranged by size: 3079 1430 988 332 281 275 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6631 HpaI GTT'AAC Cuts at: 3643 4221 3643 Size: 578 6053 KpnI G_GTAC'C Cuts at: 3956 3956 Size: 6631 MfeI C'AATT_G Cuts at: 3467 4230 3467 Size: 763 5868 MluI A'CGCG_T Cuts at: 6507 6507 Size: 6631 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5745 5929 1305 Size: 4440 184 2007 Fragments arranged by size: 4440 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4809 Fragments arranged by size: 4809 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4589 4948 5107 518 Size: 648 3423 359 159 2042 Fragments arranged by size: 3423 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 3688 4047 4724 5872 6117 1606 Size: 422 1660 359 677 1148 245 2120 Fragments arranged by size: 2120 1660 1148 677 422 359 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6079 NdeI CA'TA_TG Cuts at: 387 387 Size: 6631 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6631 NotI GC'GGCC_GC Cuts at: 4078 4078 Size: 6631 NsiI A_TGCA'T Cuts at: 2667 3848 2667 Size: 1181 5450 NspI r_CATG'y Cuts at: 1132 1229 3782 3850 3989 5534 1132 Size: 97 2553 68 139 1545 2229 Fragments arranged by size: 2553 2229 1545 139 97 68 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6631 PciI A'CATG_T Cuts at: 3778 5530 3778 Size: 1752 4879 PflMI CCAn_nnn'nTGG Cuts at: 1108 2123 1108 Size: 1015 5616 PfoI T'CCnGG_A Cuts at: 2201 2201 Size: 6631 PmeI GTTT'AAAC Cuts at: 3935 3935 Size: 6631 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4700 4732 6213 6245 4700 Size: 32 1481 32 5086 Fragments arranged by size: 5086 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 3160 2248 Size: 912 5719 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6631 PsiI TTA'TAA Cuts at: 4201 4201 Size: 6631 PstI C_TGCA'G Cuts at: 830 2757 3983 830 Size: 1927 1226 3478 Fragments arranged by size: 3478 1927 1226 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6631 SalI G'TCGA_C Cuts at: 1936 3973 1936 Size: 2037 4594 SbfI CC_TGCA'GG Cuts at: 3983 3983 Size: 6631 ScaI AGT'ACT Cuts at: 3086 3637 4879 6591 3086 Size: 551 1242 1712 3126 Fragments arranged by size: 3126 1712 1242 551 SfcI C'TryA_G Cuts at: 826 1045 2753 3059 3789 3979 5114 5795 5986 Size: 219 1708 306 730 190 1135 681 191 Cuts at: 5986 826 Size: 1471 Fragments arranged by size: 1708 1471 1135 730 681 306 219 191 190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6631 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6631 SmaI CCC'GGG Cuts at: 1872 3958 1872 Size: 2086 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3369 3386 3599 3803 4735 5636 Size: 197 926 1426 17 213 204 932 901 Cuts at: 5636 5898 820 Size: 262 1553 Fragments arranged by size: 1553 1426 932 926 901 262 213 204 197 17 SnaBI TAC'GTA Cuts at: 493 4422 493 Size: 3929 2702 SpeI A'CTAG_T Cuts at: 152 2186 3700 152 Size: 2034 1514 3083 Fragments arranged by size: 3083 2034 1514 SphI G_CATG'C Cuts at: 1132 3989 1132 Size: 2857 3774 SspI AAT'ATT Cuts at: 5 52 3871 4555 5 Size: 47 3819 684 2081 Fragments arranged by size: 3819 2081 684 47 StuI AGG'CCT Cuts at: 1517 2088 1517 Size: 571 6060 StyI C'CwwG_G Cuts at: 513 1065 2476 2633 3156 3606 4070 513 Size: 552 1411 157 523 450 464 3074 Fragments arranged by size: 3074 1411 552 523 464 450 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4658 4675 4828 5013 5458 325 Size: 1255 3078 17 153 185 445 1498 Fragments arranged by size: 3078 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2511 3084 3635 Size: 274 80 33 51 1443 534 573 551 Cuts at: 3635 4877 6589 96 Size: 1242 1712 138 Fragments arranged by size: 1712 1443 1242 573 551 534 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6591 6623 6591 Size: 32 6599 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6631 XbaI T'CTAG_A Cuts at: 2881 3967 2881 Size: 1086 5545 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2515 2987 2515 Size: 472 6159 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6631 XmaI C'CCGG_G Cuts at: 1870 3956 1870 Size: 2086 4545 XmnI GAAnn'nnTTC Cuts at: 2387 2454 3522 4760 2387 Size: 67 1068 1238 4258 Fragments arranged by size: 4258 1238 1068 67 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4439 276 Size: 53 83 186 936 2905 2468 Fragments arranged by size: 2905 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI