(Circular) (Six-base) MAPSORT of: pFN21AE1052.seq Check: 7091 from: 1 to: 5830 pFN21AE1052 5830 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2556 3173 1937 Size: 619 617 4594 Fragments arranged by size: 4594 619 617 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3893 5277 5545 1632 Size: 2261 1384 268 1917 Fragments arranged by size: 2261 1917 1384 268 AfeI AGC'GCT Cuts at: 2049 2375 2049 Size: 326 5504 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 2727 1166 Size: 1561 4269 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2900 2932 2900 Size: 32 5798 AlwNI CAG_nnn'CTG Cuts at: 2433 3573 5145 2433 Size: 1140 1572 3118 Fragments arranged by size: 3118 1572 1140 ApaLI G'TGCA_C Cuts at: 2039 3887 5043 2039 Size: 1848 1156 2826 Fragments arranged by size: 2826 1848 1156 ApoI r'AATT_y Cuts at: 1493 2148 2423 2633 3139 3365 3517 5626 1493 Size: 655 275 210 506 226 152 2109 1697 Fragments arranged by size: 2109 1697 655 506 275 226 210 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 3155 1870 Size: 73 1212 4545 Fragments arranged by size: 4545 1212 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3151 4606 618 Size: 325 217 627 1364 1455 1842 Fragments arranged by size: 1842 1455 1364 627 325 217 BanII G_rGCy'C Cuts at: 1391 2489 2901 3149 1391 Size: 1098 412 248 4072 Fragments arranged by size: 4072 1098 412 248 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2021 3045 928 Size: 971 122 1024 3713 Fragments arranged by size: 3713 1024 971 122 BbvCI CC'TCA_GC Cuts at: 2354 2354 Size: 5830 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4021 4055 1903 Size: 34 2084 34 3678 Fragments arranged by size: 3678 2084 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4441 136 Size: 107 122 71 631 605 2769 1525 Fragments arranged by size: 2769 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2762 2769 5825 2762 Size: 7 3056 2767 Fragments arranged by size: 3056 2767 7 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 2043 3891 5047 1790 Size: 253 1848 1156 2573 Fragments arranged by size: 2573 1848 1156 253 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2802 3005 3561 Size: 416 53 83 399 24 1385 203 556 Cuts at: 3561 4519 442 Size: 958 1753 Fragments arranged by size: 1753 1385 958 556 416 399 203 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 4490 1239 Size: 833 2418 2579 Fragments arranged by size: 2579 2418 833 Bpu10I CC'TnA_GC Cuts at: 2354 2354 Size: 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3955 4820 5118 3955 Size: 865 298 4667 Fragments arranged by size: 4667 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4493 882 Size: 617 2994 2219 Fragments arranged by size: 2994 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2797 3621 493 Size: 711 1593 824 2702 Fragments arranged by size: 2702 1593 824 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3637 4019 4654 275 Size: 53 83 186 936 2104 382 635 1451 Fragments arranged by size: 2104 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2900 2930 1178 Size: 30 165 30 1497 30 4078 Fragments arranged by size: 4078 1497 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5830 BseYI C'CCAG_C Cuts at: 2433 2537 2638 2653 5033 5529 2433 Size: 104 101 15 2380 496 2734 Fragments arranged by size: 2734 2380 496 104 101 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2459 2866 1163 Size: 573 723 407 4127 Fragments arranged by size: 4127 723 573 407 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 2724 2880 3891 3976 5047 2043 Size: 681 156 1011 85 1071 2826 Fragments arranged by size: 2826 1071 1011 681 156 85 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 BsmBI CGTCTCn'nnnn_ Cuts at: 3041 3041 Size: 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2489 2724 2880 2901 3149 3891 Size: 399 253 446 235 156 21 248 742 Cuts at: 3891 3976 5047 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 446 399 253 248 235 156 85 21 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 2205 3719 5449 2205 Size: 1514 1730 2586 Fragments arranged by size: 2586 1730 1514 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3185 844 Size: 2341 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4325 4499 65 Size: 1183 3077 174 1396 Fragments arranged by size: 3077 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4474 4661 1700 Size: 110 2664 187 2869 Fragments arranged by size: 2869 2664 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 3007 3890 4902 3007 Size: 883 1012 3935 Fragments arranged by size: 3935 1012 883 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5830 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 2596 1184 Size: 1412 4418 BstYI r'GATC_y Cuts at: 1972 2649 2762 2769 2925 3160 3527 3912 3929 Size: 677 113 7 156 235 367 385 17 Cuts at: 3929 5381 5467 5479 5825 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 677 385 367 346 235 156 113 86 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5278 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 3075 3346 4140 4160 3075 Size: 271 794 20 4745 Fragments arranged by size: 4745 794 271 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2605 4837 809 Size: 507 1289 2232 1802 Fragments arranged by size: 2232 1802 1289 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2065 2380 2610 Size: 54 1425 50 275 18 235 315 230 Cuts at: 2610 3277 4166 5795 8 Size: 667 889 1629 43 Fragments arranged by size: 1629 1425 889 667 315 275 235 230 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3760 1353 Size: 2407 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2762 4417 4791 4937 387 Size: 1064 138 1173 1655 374 146 1280 Fragments arranged by size: 1655 1280 1173 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 3893 4490 5277 5545 1239 Size: 393 440 1821 597 787 268 1524 Fragments arranged by size: 1821 1524 787 597 440 393 268 EcoRI G'AATT_C Cuts at: 2423 2633 3139 3517 2423 Size: 210 506 378 4736 Fragments arranged by size: 4736 506 378 210 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2710 2710 Size: 5830 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 2051 2377 4977 1678 Size: 373 326 2600 2531 Fragments arranged by size: 2600 2531 373 326 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 2754 2786 4471 Size: 32 135 32 584 32 566 32 1685 Cuts at: 4471 4503 4545 4577 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1685 584 566 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2388 2398 3174 3420 669 Size: 988 281 450 10 776 246 3079 Fragments arranged by size: 3079 988 776 450 281 246 10 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4944 5128 1305 Size: 3639 184 2007 Fragments arranged by size: 3639 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2067 10 Size: 54 1425 343 235 3773 Fragments arranged by size: 3773 1425 343 235 54 MslI CAynn'nnrTG Cuts at: 518 1166 2727 2873 3032 3788 4147 4306 518 Size: 648 1561 146 159 756 359 159 2042 Fragments arranged by size: 2042 1561 756 648 359 159 159 146 MspA1I CmG'CkG Cuts at: 1606 2430 2451 2788 3246 3923 5071 5316 1606 Size: 824 21 337 458 677 1148 245 2120 Fragments arranged by size: 2120 1148 824 677 458 337 245 21 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5278 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 3188 4733 1132 Size: 97 1959 1545 2229 Fragments arranged by size: 2229 1959 1545 97 PasI CC'CwG_GG Cuts at: 1348 2933 1348 Size: 1585 4245 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 2660 2731 1108 Size: 1552 71 4207 Fragments arranged by size: 4207 1552 71 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2900 2932 3899 3931 5412 5444 2900 Size: 32 967 32 1481 32 3286 Fragments arranged by size: 3286 1481 967 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2393 1613 Size: 780 5050 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2511 2543 2511 Size: 32 5798 PstI C_TGCA'G Cuts at: 830 2450 2465 2744 3182 830 Size: 1620 15 279 438 3478 Fragments arranged by size: 3478 1620 438 279 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 PvuII CAG'CTG Cuts at: 2430 2788 2430 Size: 358 5472 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2277 2446 2461 2740 3178 4313 4994 Size: 219 1232 169 15 279 438 1135 681 Cuts at: 4994 5185 826 Size: 191 1471 Fragments arranged by size: 1471 1232 1135 681 438 279 219 191 169 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3934 4835 5097 820 Size: 197 926 1991 901 262 1553 Fragments arranged by size: 1991 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 2953 1517 Size: 1436 4394 StyI C'CwwG_G Cuts at: 513 1065 2643 3269 513 Size: 552 1578 626 3074 Fragments arranged by size: 3074 1578 626 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2298 2425 3857 3874 4027 4212 4657 Size: 1255 718 127 1432 17 153 185 445 Cuts at: 4657 325 Size: 1498 Fragments arranged by size: 1498 1432 1255 718 445 185 153 127 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4076 5788 96 Size: 274 80 33 51 1443 2099 1712 138 Fragments arranged by size: 2099 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5790 5822 5790 Size: 32 5798 Tth111I GACn'n_nGTC Cuts at: 1316 2197 1316 Size: 881 4949 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI