(Circular) (Six-base) MAPSORT of: pFN21AE1050.seq Check: 203 from: 1 to: 6133 pFN21AE1050 6133 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3104 3104 Size: 6133 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3476 1937 Size: 1539 4594 Acc65I G'GTAC_C Cuts at: 3454 3454 Size: 6133 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2432 4196 5580 5848 1632 Size: 800 1764 1384 268 1917 Fragments arranged by size: 1917 1764 1384 800 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 5032 6009 1203 Size: 3829 977 1327 Fragments arranged by size: 3829 1327 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 3098 1166 Size: 1932 4201 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AlwNI CAG_nnn'CTG Cuts at: 3390 3876 5448 3390 Size: 486 1572 4075 Fragments arranged by size: 4075 1572 486 ApaI G_GGCC'C Cuts at: 2016 2459 2586 2597 2736 2941 2016 Size: 443 127 11 139 205 5208 Fragments arranged by size: 5208 443 205 139 127 11 ApaLI G'TGCA_C Cuts at: 4190 5346 4190 Size: 1156 4977 ApoI r'AATT_y Cuts at: 1493 3442 3668 3820 5929 1493 Size: 1949 226 152 2109 1697 Fragments arranged by size: 2109 1949 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 2086 2590 3458 1870 Size: 73 143 504 868 4545 Fragments arranged by size: 4545 868 504 143 73 BamHI G'GATC_C Cuts at: 3051 3463 3830 3051 Size: 412 367 5354 Fragments arranged by size: 5354 412 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 2740 3454 4909 618 Size: 325 217 627 294 659 714 1455 1842 Fragments arranged by size: 1842 1455 714 659 627 325 294 217 BanII G_rGCy'C Cuts at: 1391 2016 2046 2056 2247 2379 2408 2459 2586 Size: 625 30 10 191 132 29 51 127 Cuts at: 2586 2597 2736 2941 3408 3452 1391 Size: 11 139 205 467 44 4072 Fragments arranged by size: 4072 625 467 205 191 139 132 127 51 44 30 29 11 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4324 4358 1903 Size: 34 2387 34 3678 Fragments arranged by size: 3678 2387 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 1548 Size: 6133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2020 2573 3123 Size: 107 122 71 631 605 348 553 550 Cuts at: 3123 4744 136 Size: 1621 1525 Fragments arranged by size: 1621 1525 631 605 553 550 348 122 107 71 BglII A'GATC_T Cuts at: 6128 6128 Size: 6133 BlpI GC'TnA_GC Cuts at: 2303 3550 2303 Size: 1247 4886 Bme1580I G_kGCm'C Cuts at: 1790 2016 2459 2586 2597 2736 2745 2941 3366 Size: 226 443 127 11 139 9 196 425 Cuts at: 3366 4194 5350 1790 Size: 828 1156 2573 Fragments arranged by size: 2573 1156 828 443 425 226 196 139 127 11 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3864 4822 442 Size: 416 53 83 399 24 2447 958 1753 Fragments arranged by size: 2447 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2132 2772 2132 Size: 640 5493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2213 2448 2742 3032 4793 1239 Size: 863 111 235 294 290 1761 2579 Fragments arranged by size: 2579 1761 863 294 290 235 111 Bpu10I CC'TnA_GC Cuts at: 2747 3228 2747 Size: 481 5652 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4258 5123 5421 4258 Size: 865 298 4970 Fragments arranged by size: 4970 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2698 4796 882 Size: 617 1199 2098 2219 Fragments arranged by size: 2219 2098 1199 617 BsaAI yAC'GTr Cuts at: 493 1204 2908 3001 3027 3924 493 Size: 711 1704 93 26 897 2702 Fragments arranged by size: 2702 1704 897 711 93 26 BsaBI GATnn'nnATC Cuts at: 6127 6127 Size: 6133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2286 3940 4322 4957 Size: 53 83 186 936 753 1654 382 635 Cuts at: 4957 275 Size: 1451 Fragments arranged by size: 1654 1451 936 753 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2185 3048 4566 4964 5238 5385 5796 2185 Size: 863 1518 398 274 147 411 2522 Fragments arranged by size: 2522 1518 863 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2844 2874 1178 Size: 30 165 30 1441 30 4437 Fragments arranged by size: 4437 1441 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2733 2989 3257 3313 3317 1186 Size: 1547 256 268 56 4 4002 Fragments arranged by size: 4002 1547 268 256 56 4 BseYI C'CCAG_C Cuts at: 2437 2500 2687 2816 2876 2940 3033 5336 5832 Size: 63 187 129 60 64 93 2303 496 Cuts at: 5832 2437 Size: 2738 Fragments arranged by size: 2738 2303 496 187 129 93 64 63 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2730 3032 3408 4194 4279 5350 2730 Size: 302 376 786 85 1071 3513 Fragments arranged by size: 3513 1071 786 376 302 85 BsmI GAATG_Cn' Cuts at: 3644 3737 3644 Size: 93 6040 BsmBI CGTCTCn'nnnn_ Cuts at: 2448 2448 Size: 6133 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2046 2056 2247 2379 2408 2459 Size: 399 226 30 10 191 132 29 51 Cuts at: 2459 2586 2597 2730 2736 2745 2941 3032 3366 Size: 127 11 133 6 9 196 91 334 Cuts at: 3366 3408 3452 4194 4279 5350 1391 Size: 42 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 334 226 196 191 133 132 127 91 85 51 44 42 30 29 11 10 9 6 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 4022 5752 4022 Size: 1730 4403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3104 3488 844 Size: 2260 384 3489 Fragments arranged by size: 3489 2260 384 BsrBI CCG'CTC Cuts at: 1394 1823 2434 4020 1394 Size: 429 611 1586 3507 Fragments arranged by size: 3507 1586 611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4628 4802 65 Size: 1183 3380 174 1396 Fragments arranged by size: 3380 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2098 2200 4777 4964 1700 Size: 110 288 102 2577 187 2869 Fragments arranged by size: 2869 2577 288 187 110 102 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 4193 5205 4193 Size: 1012 5121 BstAPI GCAn_nnn'nTGC Cuts at: 794 3381 794 Size: 2587 3546 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6133 BstXI CCAn_nnnn'nTGG Cuts at: 2843 2843 Size: 6133 BstYI r'GATC_y Cuts at: 1972 3051 3156 3463 3830 4215 4232 5684 5770 Size: 1079 105 307 367 385 17 1452 86 Cuts at: 5770 5782 6128 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1079 385 367 346 307 105 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2058 2058 Size: 6133 BtgI C'CryG_G Cuts at: 513 1065 2077 2104 2218 2967 3112 513 Size: 552 1012 27 114 749 145 3534 Fragments arranged by size: 3534 1012 749 552 145 114 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5394 Fragments arranged by size: 5394 683 56 BtsI GCAGTG_nn' Cuts at: 2168 3160 3649 4443 4463 2168 Size: 992 489 794 20 3838 Fragments arranged by size: 3838 992 794 489 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 3437 3437 Size: 6133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2316 2833 2950 Size: 54 1425 50 275 18 486 517 117 Cuts at: 2950 3003 3580 4469 6098 8 Size: 53 577 889 1629 43 Fragments arranged by size: 1629 1425 889 577 517 486 275 117 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2704 2710 4063 1353 Size: 1351 6 1353 3423 Fragments arranged by size: 3423 1353 1351 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4720 5094 5240 387 Size: 1064 138 3131 374 146 1280 Fragments arranged by size: 3131 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3406 3406 Size: 6133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2213 2432 2448 2742 3032 4196 Size: 393 470 111 219 16 294 290 1164 Cuts at: 4196 4793 5580 5848 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1164 787 597 470 393 294 290 268 219 111 16 EcoNI CCTnn'n_nnAGG Cuts at: 2610 3231 2610 Size: 621 5512 EcoO109I rG'GnC_Cy Cuts at: 2012 2173 2214 2263 2455 2456 2583 2594 2633 Size: 161 41 49 192 1 127 11 39 Cuts at: 2633 2733 2012 Size: 100 5412 Fragments arranged by size: 5412 192 161 127 100 49 41 39 11 1 EcoRI G'AATT_C Cuts at: 3442 3820 3442 Size: 378 5755 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 3390 5280 1678 Size: 1712 1890 2531 Fragments arranged by size: 2531 1890 1712 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2610 2642 4774 4806 4848 Size: 32 135 32 1038 32 2132 32 42 Cuts at: 4848 4880 1373 Size: 32 2626 Fragments arranged by size: 2626 2132 1038 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3477 3723 669 Size: 988 281 1539 246 3079 Fragments arranged by size: 3079 1539 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6133 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KpnI G_GTAC'C Cuts at: 3458 3458 Size: 6133 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5247 5431 1305 Size: 3942 184 2007 Fragments arranged by size: 3942 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2835 3005 10 Size: 54 1425 343 1003 170 3138 Fragments arranged by size: 3138 1425 1003 343 170 54 MslI CAynn'nnrTG Cuts at: 518 1166 3098 4091 4450 4609 518 Size: 648 1932 993 359 159 2042 Fragments arranged by size: 2042 1932 993 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2079 2820 3114 3355 3549 4226 5374 5619 Size: 473 741 294 241 194 677 1148 245 Cuts at: 5619 1606 Size: 2120 Fragments arranged by size: 2120 1148 741 677 473 294 245 241 194 NaeI GCC'GGC Cuts at: 2202 2202 Size: 6133 NcoI C'CATG_G Cuts at: 513 1065 2218 513 Size: 552 1153 4428 Fragments arranged by size: 4428 1153 552 NdeI CA'TA_TG Cuts at: 387 2371 387 Size: 1984 4149 NgoMIV G'CCGG_C Cuts at: 2200 2200 Size: 6133 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 2934 3491 5036 1132 Size: 97 1705 557 1545 2229 Fragments arranged by size: 2229 1705 1545 557 97 PasI CC'CwG_GG Cuts at: 1348 2130 2177 1348 Size: 782 47 5304 Fragments arranged by size: 5304 782 47 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6133 PfoI T'CCnGG_A Cuts at: 3042 3399 3042 Size: 357 5776 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PmlI CAC'GTG Cuts at: 2908 3027 2908 Size: 119 6014 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2214 2214 Size: 6133 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6133 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PspOMI G'GGCC_C Cuts at: 2012 2455 2582 2593 2732 2937 2012 Size: 443 127 11 139 205 5208 Fragments arranged by size: 5208 443 205 139 127 11 PstI C_TGCA'G Cuts at: 830 2254 2707 3386 3485 830 Size: 1424 453 679 99 3478 Fragments arranged by size: 3478 1424 679 453 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 SacI G_AGCT'C Cuts at: 3408 3408 Size: 6133 SacII CC_GC'GG Cuts at: 2080 3115 2080 Size: 1035 5098 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SanDI GG'GwC_CC Cuts at: 2214 2214 Size: 6133 SbfI CC_TGCA'GG Cuts at: 2707 3485 2707 Size: 778 5355 ScaI AGT'ACT Cuts at: 2226 2828 4381 6093 2226 Size: 602 1553 1712 2266 Fragments arranged by size: 2266 1712 1553 602 SfcI C'TryA_G Cuts at: 826 1045 2250 2641 2703 3382 3481 4616 5297 Size: 219 1205 391 62 679 99 1135 681 Cuts at: 5297 5488 826 Size: 191 1471 Fragments arranged by size: 1471 1205 1135 681 679 391 219 191 99 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 2088 2592 3460 1872 Size: 216 504 868 4545 Fragments arranged by size: 4545 868 504 216 SmlI C'TyrA_G Cuts at: 820 1017 1943 4237 5138 5400 820 Size: 197 926 2294 901 262 1553 Fragments arranged by size: 2294 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 3491 1132 Size: 2359 3774 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 2576 3074 3380 1517 Size: 1059 498 306 4270 Fragments arranged by size: 4270 1059 498 306 StyI C'CwwG_G Cuts at: 513 1065 2218 3572 513 Size: 552 1153 1354 3074 Fragments arranged by size: 3074 1354 1153 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2197 2376 2569 4160 4177 4330 4515 Size: 1255 617 179 193 1591 17 153 185 Cuts at: 4515 4960 325 Size: 445 1498 Fragments arranged by size: 1591 1498 1255 617 445 193 185 179 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2224 2826 4379 Size: 274 80 33 51 1443 247 602 1553 Cuts at: 4379 6091 96 Size: 1712 138 Fragments arranged by size: 1712 1553 1443 602 274 247 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2876 6093 6125 2844 Size: 32 3217 32 2852 Fragments arranged by size: 3217 2852 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6133 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 2086 2590 3458 1870 Size: 216 504 868 4545 Fragments arranged by size: 4545 868 504 216 XmnI GAAnn'nnTTC Cuts at: 4262 4262 Size: 6133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI PvuII RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI