Restriction map for pFN21AE1050
 (Linear) (Six-base) MAPSORT of: KIEE1050.seq  Check: 6417  from: 1  to: 1428



KIEE1050.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1099    1428 
   Size:       1099     329
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427    1428 
   Size:        427    1001
 
AleI CACnn'nnGTG

Cuts at:      0    1093    1428 
   Size:       1093     335
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1385    1428 
   Size:       1385      43
 
ApaI G_GGCC'C

Cuts at:      0      11     454     581     592     731     936    1428 
   Size:         11     443     127      11     139     205     492

  Fragments arranged by size: 

                 492     443     205     139     127      11      11
 
AvaI C'yCGr_G

Cuts at:      0      81     585    1428 
   Size:         81     504     843

  Fragments arranged by size: 

                 843     504      81
 
BamHI G'GATC_C

Cuts at:      0    1046    1428 
   Size:       1046     382
 
BanI G'GyrC_C

Cuts at:      0      76     735    1428 
   Size:         76     659     693

  Fragments arranged by size: 

                 693     659      76
 
BanII G_rGCy'C

Cuts at:      0      11      41      51     242     374     403     454     581 
   Size:         11      30      10     191     132      29      51     127

Cuts at:    581     592     731     936    1403    1428 
   Size:         11     139     205     467      25

  Fragments arranged by size: 

                 467     205     191     139     132     127      51      30
                  29      25      11      11      10
 
BglI GCCn_nnn'nGGC

Cuts at:      0      15     568    1118    1428 
   Size:         15     553     550     310

  Fragments arranged by size: 

                 553     550     310      15
 
BlpI GC'TnA_GC

Cuts at:      0     298    1428 
   Size:        298    1130
 
Bme1580I G_kGCm'C

Cuts at:      0      11     454     581     592     731     740     936    1361 
   Size:         11     443     127      11     139       9     196     425

Cuts at:   1361    1428 
   Size:         67

  Fragments arranged by size: 

                 443     425     196     139     127      67      11      11
                   9
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     127     767    1428 
   Size:        127     640     661

  Fragments arranged by size: 

                 661     640     127
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     208     443     737    1027    1428 
   Size:         97     111     235     294     290     401

  Fragments arranged by size: 

                 401     294     290     235     111      97
 
Bpu10I CC'TnA_GC

Cuts at:      0     742    1223    1428 
   Size:        742     481     205

  Fragments arranged by size: 

                 742     481     205
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     693    1428 
   Size:        693     735
 
BsaAI yAC'GTr

Cuts at:      0     903     996    1022    1428 
   Size:        903      93      26     406

  Fragments arranged by size: 

                 903     406      93      26
 
BsaHI Gr'CG_yC

Cuts at:      0     281    1428 
   Size:        281    1147
 
BsaWI w'CCGG_w

Cuts at:      0     180    1043    1428 
   Size:        180     863     385

  Fragments arranged by size: 

                 863     385     180
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     839     869    1428 
   Size:        839      30     559

  Fragments arranged by size: 

                 839     559      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     728     984    1252    1308    1312    1428 
   Size:        728     256     268      56       4     116

  Fragments arranged by size: 

                 728     268     256     116      56       4
 
BseYI C'CCAG_C

Cuts at:      0     432     495     682     811     871     935    1028    1428 
   Size:        432      63     187     129      60      64      93     400

  Fragments arranged by size: 

                 432     400     187     129      93      64      63      60
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     725    1027    1403    1428 
   Size:        725     302     376      25

  Fragments arranged by size: 

                 725     376     302      25
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     443    1428 
   Size:        443     985
 
Bsp1286I G_dGCh'C

Cuts at:      0      11      41      51     242     374     403     454     581 
   Size:         11      30      10     191     132      29      51     127

Cuts at:    581     592     725     731     740     936    1027    1361    1403 
   Size:         11     133       6       9     196      91     334      42

Cuts at:   1403    1428 
   Size:         25

  Fragments arranged by size: 

                 334     196     191     133     132     127      91      51
                  42      30      29      25      11      11      10       9
                   6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1099    1428 
   Size:       1099     329
 
BsrBI CCG'CTC

Cuts at:      0     429    1428 
   Size:        429     999
 
BsrFI r'CCGG_y

Cuts at:      0      93     195    1428 
   Size:         93     102    1233

  Fragments arranged by size: 

                1233     102      93
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1376    1428 
   Size:       1376      52
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     838    1428 
   Size:        838     590
 
BstYI r'GATC_y

Cuts at:      0    1046    1151    1428 
   Size:       1046     105     277

  Fragments arranged by size: 

                1046     277     105
 
Bsu36I CC'TnA_GG

Cuts at:      0      53    1428 
   Size:         53    1375
 
BtgI C'CryG_G

Cuts at:      0      72      99     213     962    1107    1428 
   Size:         72      27     114     749     145     321

  Fragments arranged by size: 

                 749     321     145     114      72      27
 
BtsI GCAGTG_nn'

Cuts at:      0     163    1155    1428 
   Size:        163     992     273

  Fragments arranged by size: 

                 992     273     163
 
EaeI y'GGCC_r

Cuts at:      0     311     828     945     998    1428 
   Size:        311     517     117      53     430

  Fragments arranged by size: 

                 517     430     311     117      53
 
EarI CTCTTCn'nnn_

Cuts at:      0     699     705    1428 
   Size:        699       6     723

  Fragments arranged by size: 

                 723     699       6
 
EcoICRI GAG'CTC

Cuts at:      0    1401    1428 
   Size:       1401      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     208     427     443     737    1027    1428 
   Size:         97     111     219      16     294     290     401

  Fragments arranged by size: 

                 401     294     290     219     111      97      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     605    1226    1428 
   Size:        605     621     202

  Fragments arranged by size: 

                 621     605     202
 
EcoO109I rG'GnC_Cy

Cuts at:      0       7     168     209     258     450     451     578     589 
   Size:          7     161      41      49     192       1     127      11

Cuts at:    589     628     728    1428 
   Size:         39     100     700

  Fragments arranged by size: 

                 700     192     161     127     100      49      41      39
                  11       7       1
 
HaeII r_GCGC'y

Cuts at:      0    1385    1428 
   Size:       1385      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     605     637    1428 
   Size:        605      32     791

  Fragments arranged by size: 

                 791     605      32
 
MscI TGG'CCA

Cuts at:      0     830    1000    1428 
   Size:        830     170     428

  Fragments arranged by size: 

                 830     428     170
 
MslI CAynn'nnrTG

Cuts at:      0    1093    1428 
   Size:       1093     335
 
MspA1I CmG'CkG

Cuts at:      0      74     815    1109    1350    1428 
   Size:         74     741     294     241      78

  Fragments arranged by size: 

                 741     294     241      78      74
 
NaeI GCC'GGC

Cuts at:      0     197    1428 
   Size:        197    1231
 
NcoI C'CATG_G

Cuts at:      0     213    1428 
   Size:        213    1215
 
NdeI CA'TA_TG

Cuts at:      0     366    1428 
   Size:        366    1062
 
NgoMIV G'CCGG_C

Cuts at:      0     195    1428 
   Size:        195    1233
 
NspI r_CATG'y

Cuts at:      0     929    1428 
   Size:        929     499
 
PasI CC'CwG_GG

Cuts at:      0     125     172    1428 
   Size:        125      47    1256

  Fragments arranged by size: 

                1256     125      47
 
PfoI T'CCnGG_A

Cuts at:      0    1037    1394    1428 
   Size:       1037     357      34

  Fragments arranged by size: 

                1037     357      34
 
PmlI CAC'GTG

Cuts at:      0     903    1022    1428 
   Size:        903     119     406

  Fragments arranged by size: 

                 903     406     119
 
PpuMI rG'GwC_Cy

Cuts at:      0     209    1428 
   Size:        209    1219
 
PspOMI G'GGCC_C

Cuts at:      0       7     450     577     588     727     932    1428 
   Size:          7     443     127      11     139     205     496

  Fragments arranged by size: 

                 496     443     205     139     127      11       7
 
PstI C_TGCA'G

Cuts at:      0     249     702    1381    1428 
   Size:        249     453     679      47

  Fragments arranged by size: 

                 679     453     249      47
 
SacI G_AGCT'C

Cuts at:      0    1403    1428 
   Size:       1403      25
 
SacII CC_GC'GG

Cuts at:      0      75    1110    1428 
   Size:         75    1035     318

  Fragments arranged by size: 

                1035     318      75
 
SanDI GG'GwC_CC

Cuts at:      0     209    1428 
   Size:        209    1219
 
SbfI CC_TGCA'GG

Cuts at:      0     702    1428 
   Size:        702     726
 
ScaI AGT'ACT

Cuts at:      0     221     823    1428 
   Size:        221     602     605

  Fragments arranged by size: 

                 605     602     221
 
SfcI C'TryA_G

Cuts at:      0     245     636     698    1377    1428 
   Size:        245     391      62     679      51

  Fragments arranged by size: 

                 679     391     245      62      51
 
SmaI CCC'GGG

Cuts at:      0      83     587    1428 
   Size:         83     504     841

  Fragments arranged by size: 

                 841     504      83
 
StuI AGG'CCT

Cuts at:      0     571    1069    1375    1428 
   Size:        571     498     306      53

  Fragments arranged by size: 

                 571     498     306      53
 
StyI C'CwwG_G

Cuts at:      0     213    1428 
   Size:        213    1215
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     192     371     564    1428 
   Size:        192     179     193     864

  Fragments arranged by size: 

                 864     193     192     179
 
TatI w'GTAC_w

Cuts at:      0     219     821    1428 
   Size:        219     602     607

  Fragments arranged by size: 

                 607     602     219
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     839     871    1428 
   Size:        839      32     557

  Fragments arranged by size: 

                 839     557      32
 
XmaI C'CCGG_G

Cuts at:      0      81     585    1428 
   Size:         81     504     843

  Fragments arranged by size: 

                 843     504      81
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApaI     AvaI    BamHI     BanI
    BanII     BglI     BlpI Bme1580I     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I     MscI     MslI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NspI     PasI     PfoI     PmlI    PpuMI   PspOMI     PstI
     SacI    SacII    SanDI     SbfI     ScaI     SfcI     SmaI     StuI
     StyI    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII    BmgBI     BmrI     BmtI    BpuEI    BsaBI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NarI     NheI     NotI     NruI     NsiI     PacI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SalI     SapI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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