(Circular) (Six-base) MAPSORT of: pFN21AE1047.seq Check: 7268 from: 1 to: 5743 pFN21AE1047 5743 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2979 2979 Size: 5743 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2418 3086 1937 Size: 481 668 4594 Fragments arranged by size: 4594 668 481 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2498 3806 5190 5458 1632 Size: 866 1308 1384 268 1917 Fragments arranged by size: 1917 1384 1308 866 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 2839 4642 5619 1203 Size: 1636 1803 977 1327 Fragments arranged by size: 1803 1636 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 2248 2716 2881 3486 5058 2248 Size: 468 165 605 1572 2933 Fragments arranged by size: 2933 1572 605 468 165 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 2669 3052 3278 3430 5539 1493 Size: 1176 383 226 152 2109 1697 Fragments arranged by size: 2109 1697 1176 383 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2135 2613 3068 1870 Size: 73 192 478 455 4545 Fragments arranged by size: 4545 478 455 192 73 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3064 4519 618 Size: 325 217 627 1277 1455 1842 Fragments arranged by size: 1842 1455 1277 627 325 217 BanII G_rGCy'C Cuts at: 1391 2759 3062 1391 Size: 1368 303 4072 Fragments arranged by size: 4072 1368 303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2348 2439 928 Size: 971 449 91 4232 Fragments arranged by size: 4232 971 449 91 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2696 4354 136 Size: 107 122 71 631 605 1024 1658 1525 Fragments arranged by size: 1658 1525 1024 631 605 122 107 71 BglII A'GATC_T Cuts at: 2538 5738 2538 Size: 3200 2543 BlpI GC'TnA_GC Cuts at: 2738 3160 2738 Size: 422 5321 Bme1580I G_kGCm'C Cuts at: 1790 3804 4960 1790 Size: 2014 1156 2573 Fragments arranged by size: 2573 2014 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3028 3474 4432 Size: 416 53 83 399 24 1611 446 958 Cuts at: 4432 442 Size: 1753 Fragments arranged by size: 1753 1611 958 446 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2113 2423 2430 4403 1239 Size: 874 310 7 1973 2579 Fragments arranged by size: 2579 1973 874 310 7 Bpu10I CC'TnA_GC Cuts at: 2249 2249 Size: 5743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3868 4733 5031 3868 Size: 865 298 4580 Fragments arranged by size: 4580 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4406 882 Size: 617 2907 2219 Fragments arranged by size: 2907 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2554 3534 493 Size: 711 1350 980 2702 Fragments arranged by size: 2702 1350 980 711 BsaBI GATnn'nnATC Cuts at: 2013 2543 5737 2013 Size: 530 3194 2019 Fragments arranged by size: 3194 2019 530 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2436 3550 3932 4567 Size: 53 83 186 936 903 1114 382 635 Cuts at: 4567 275 Size: 1451 Fragments arranged by size: 1451 1114 936 903 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5518 Fragments arranged by size: 5518 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2810 2971 1186 Size: 1624 161 3958 Fragments arranged by size: 3958 1624 161 BseYI C'CCAG_C Cuts at: 2346 2472 2821 2848 2935 4946 5442 2346 Size: 126 349 27 87 2011 496 2647 Fragments arranged by size: 2647 2011 496 349 126 87 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 2396 2410 1163 Size: 573 456 204 14 4496 Fragments arranged by size: 4496 573 456 204 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2553 2958 3804 3889 4960 2553 Size: 405 846 85 1071 3336 Fragments arranged by size: 3336 1071 846 405 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2553 2759 2958 3062 3804 3889 4960 Size: 399 763 206 199 104 742 85 1071 Cuts at: 4960 1391 Size: 2174 Fragments arranged by size: 2174 1071 763 742 399 206 199 104 85 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2979 3098 844 Size: 2135 119 3489 Fragments arranged by size: 3489 2135 119 BsrBI CCG'CTC Cuts at: 1394 1823 2224 3630 1394 Size: 429 401 1406 3507 Fragments arranged by size: 3507 1406 429 401 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2901 4387 4574 1700 Size: 110 1091 1486 187 2869 Fragments arranged by size: 2869 1486 1091 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5743 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5743 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5743 BstYI r'GATC_y Cuts at: 1972 2014 2538 3073 3440 3825 3842 5294 5380 Size: 42 524 535 367 385 17 1452 86 Cuts at: 5380 5392 5738 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 535 524 385 367 346 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2284 2384 2480 2790 513 Size: 552 939 280 100 96 310 3466 Fragments arranged by size: 3466 939 552 310 280 100 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 2198 2749 3259 4053 4073 2198 Size: 551 510 794 20 3868 Fragments arranged by size: 3868 794 551 510 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 2556 3190 Size: 54 1425 50 275 18 489 237 634 Cuts at: 3190 4079 5708 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 634 489 275 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2105 2910 3673 1353 Size: 752 805 763 3423 Fragments arranged by size: 3423 805 763 752 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2677 4330 4704 4850 387 Size: 1064 138 1088 1653 374 146 1280 Fragments arranged by size: 1653 1280 1088 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2113 2423 2430 2498 3806 4403 5190 Size: 393 481 310 7 68 1308 597 787 Cuts at: 5190 5458 1239 Size: 268 1524 Fragments arranged by size: 1524 1308 787 597 481 393 310 268 68 7 EcoO109I rG'GnC_Cy Cuts at: 2596 3030 2596 Size: 434 5309 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FspI TGC'GCA Cuts at: 2489 4249 2489 Size: 1760 3983 HaeII r_GCGC'y Cuts at: 1678 2248 2357 2455 4890 1678 Size: 570 109 98 2435 2531 Fragments arranged by size: 2531 2435 570 109 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4384 4416 4458 4490 1373 Size: 32 135 32 2812 32 42 32 2626 Fragments arranged by size: 2812 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2556 4857 5041 1305 Size: 1251 2301 184 2007 Fragments arranged by size: 2301 2007 1251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3921 Fragments arranged by size: 3921 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3701 4060 4219 518 Size: 648 2535 359 159 2042 Fragments arranged by size: 2535 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2206 2286 2350 2386 2482 2664 2821 3159 Size: 600 80 64 36 96 182 157 338 Cuts at: 3159 3836 4984 5229 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 600 338 245 182 157 96 80 64 36 NcoI C'CATG_G Cuts at: 513 1065 2004 2790 513 Size: 552 939 786 3466 Fragments arranged by size: 3466 939 786 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 3101 4646 1132 Size: 97 1872 1545 2229 Fragments arranged by size: 2229 1872 1545 97 PasI CC'CwG_GG Cuts at: 1348 2694 2716 1348 Size: 1346 22 4375 Fragments arranged by size: 4375 1346 22 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2855 2881 1108 Size: 1747 26 3970 Fragments arranged by size: 3970 1747 26 PfoI T'CCnGG_A Cuts at: 2500 2500 Size: 5743 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PmlI CAC'GTG Cuts at: 2554 2554 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2596 3030 2596 Size: 434 5309 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PstI C_TGCA'G Cuts at: 830 2205 2361 2460 2712 3095 830 Size: 1375 156 99 252 383 3478 Fragments arranged by size: 3478 1375 383 252 156 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 PvuII CAG'CTG Cuts at: 2206 2350 2206 Size: 144 5599 SacII CC_GC'GG Cuts at: 2287 2387 2483 2287 Size: 100 96 5547 Fragments arranged by size: 5547 100 96 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SanDI GG'GwC_CC Cuts at: 3030 3030 Size: 5743 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 3991 5703 3991 Size: 1712 4031 SexAI A'CCwGG_T Cuts at: 2775 2775 Size: 5743 SfcI C'TryA_G Cuts at: 826 1045 2201 2357 2456 2708 3091 4226 4907 Size: 219 1156 156 99 252 383 1135 681 Cuts at: 4907 5098 826 Size: 191 1471 Fragments arranged by size: 1471 1156 1135 681 383 252 219 191 156 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 3070 1872 Size: 1198 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3847 4748 5010 820 Size: 197 926 1904 901 262 1553 Fragments arranged by size: 1904 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 2400 2684 1517 Size: 883 284 4576 Fragments arranged by size: 4576 883 284 StyI C'CwwG_G Cuts at: 513 1065 2004 2790 2854 3182 513 Size: 552 939 786 64 328 3074 Fragments arranged by size: 3074 939 786 552 328 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3770 3787 3940 4125 4570 325 Size: 1255 2190 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3989 5701 96 Size: 274 80 33 51 1443 2012 1712 138 Fragments arranged by size: 2012 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2482 2514 5703 5735 2482 Size: 32 3189 32 2490 Fragments arranged by size: 3189 2490 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3034 1316 Size: 1718 4025 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 3068 1870 Size: 1198 4545 XmnI GAAnn'nnTTC Cuts at: 3872 3872 Size: 5743 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI XcmI