(Circular) (Six-base) MAPSORT of: pFN21AE3040.seq Check: 1614 from: 1 to: 5236 pFN21AE3040 5236 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3046 278 Size: 53 83 186 936 1510 2468 Fragments arranged by size: 2468 1510 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2018 2579 1937 Size: 81 561 4594 Fragments arranged by size: 4594 561 81 Acc65I G'GTAC_C Cuts at: 2557 2557 Size: 5236 AclI AA'CG_TT Cuts at: 1563 3363 3736 1563 Size: 1800 373 3063 Fragments arranged by size: 3063 1800 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2264 3299 4683 4951 1632 Size: 632 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1035 632 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5039 AflIII A'CryG_T Cuts at: 1203 4135 5112 1203 Size: 2932 977 1327 Fragments arranged by size: 2932 1327 977 AgeI A'CCGG_T Cuts at: 4067 4067 Size: 5236 AhdI GACnn_n'nnGTC Cuts at: 3965 3965 Size: 5236 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5236 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5236 AlwNI CAG_nnn'CTG Cuts at: 2979 4551 2979 Size: 1572 3664 ApaLI G'TGCA_C Cuts at: 3293 4449 3293 Size: 1156 4080 ApoI r'AATT_y Cuts at: 1493 2195 2545 2771 2923 5032 1493 Size: 702 350 226 152 2109 1697 Fragments arranged by size: 2109 1697 702 350 226 152 AseI AT'TA_AT Cuts at: 160 1032 3790 160 Size: 872 2758 1606 Fragments arranged by size: 2758 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5236 AvaI C'yCGr_G Cuts at: 1870 1943 2351 2561 1870 Size: 73 408 210 4545 Fragments arranged by size: 4545 408 210 73 BamHI G'GATC_C Cuts at: 2566 2933 2566 Size: 367 4869 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2557 4012 618 Size: 325 217 627 770 1455 1842 Fragments arranged by size: 1842 1455 770 627 325 217 BanII G_rGCy'C Cuts at: 1391 2555 1391 Size: 1164 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2339 928 Size: 971 440 3825 Fragments arranged by size: 3825 971 440 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3427 3461 1903 Size: 34 1490 34 3678 Fragments arranged by size: 3678 1490 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3128 4338 3128 Size: 1210 4026 BclI T'GATC_A Cuts at: 1548 1548 Size: 5236 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3847 136 Size: 107 122 71 631 605 2175 1525 Fragments arranged by size: 2175 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2215 5231 2215 Size: 3016 2220 BlpI GC'TnA_GC Cuts at: 2653 2653 Size: 5236 Bme1580I G_kGCm'C Cuts at: 1790 3297 4453 1790 Size: 1507 1156 2573 Fragments arranged by size: 2573 1507 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5236 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2967 3925 442 Size: 416 53 83 399 24 1550 958 1753 Fragments arranged by size: 1753 1550 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5236 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2366 2532 2366 Size: 166 5070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3896 1239 Size: 2657 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2044 2207 3361 4226 4524 2044 Size: 163 1154 865 298 2756 Fragments arranged by size: 2756 1154 865 298 163 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2351 3899 882 Size: 617 852 1548 2219 Fragments arranged by size: 2219 1548 852 617 BsaAI yAC'GTr Cuts at: 493 1204 3027 493 Size: 711 1823 2702 Fragments arranged by size: 2702 1823 711 BsaBI GATnn'nnATC Cuts at: 5230 5230 Size: 5236 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3043 3425 4060 275 Size: 53 83 186 936 1510 382 635 1451 Fragments arranged by size: 1510 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3669 4067 4341 4488 4899 3669 Size: 398 274 147 411 4006 Fragments arranged by size: 4006 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5011 Fragments arranged by size: 5011 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5236 BseYI C'CCAG_C Cuts at: 4439 4935 4439 Size: 496 4740 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2475 1163 Size: 573 739 3924 Fragments arranged by size: 3924 739 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2686 3447 4475 1540 Size: 275 186 685 761 1028 2301 Fragments arranged by size: 2301 1028 761 685 275 186 BsiHKAI G_wGCw'C Cuts at: 2518 3297 3382 4453 2518 Size: 779 85 1071 3301 Fragments arranged by size: 3301 1071 779 85 BsmI GAATG_Cn' Cuts at: 2747 2840 2747 Size: 93 5143 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2518 2555 3297 3382 4453 1391 Size: 399 728 37 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 728 399 85 37 BspEI T'CCGG_A Cuts at: 4899 4899 Size: 5236 BspHI T'CATG_A Cuts at: 3125 4855 3125 Size: 1730 3506 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2591 844 Size: 1747 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3123 1394 Size: 429 1300 3507 Fragments arranged by size: 3507 1300 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2103 3731 3905 65 Size: 1183 855 1628 174 1396 Fragments arranged by size: 1628 1396 1183 855 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2264 2293 3880 4067 1700 Size: 110 454 29 1587 187 2869 Fragments arranged by size: 2869 1587 454 187 110 29 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5236 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5236 BssSI C'ACGA_G Cuts at: 2506 3296 4308 2506 Size: 790 1012 3434 Fragments arranged by size: 3434 1012 790 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5236 BstBI TT'CG_AA Cuts at: 4040 4040 Size: 5236 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5236 BstYI r'GATC_y Cuts at: 1972 2215 2526 2566 2933 3318 3335 4787 4873 Size: 243 311 40 367 385 17 1452 86 Cuts at: 4873 4885 5231 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 311 243 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 2487 2487 Size: 5236 BtgI C'CryG_G Cuts at: 513 1065 2004 2079 2304 2392 513 Size: 552 939 75 225 88 3357 Fragments arranged by size: 3357 939 552 225 88 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4497 Fragments arranged by size: 4497 683 56 BtsI GCAGTG_nn' Cuts at: 2443 2752 3546 3566 2443 Size: 309 794 20 4113 Fragments arranged by size: 4113 794 309 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5236 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5201 DraI TTT'AAA Cuts at: 2540 2540 Size: 5236 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4243 809 Size: 507 2927 1802 Fragments arranged by size: 2927 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2683 3572 Size: 54 1425 50 275 18 252 601 889 Cuts at: 3572 5201 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 601 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2683 1537 Size: 275 871 4090 Fragments arranged by size: 4090 871 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3166 1353 Size: 1813 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2168 3823 4197 4343 387 Size: 1064 138 579 1655 374 146 1280 Fragments arranged by size: 1655 1280 1064 579 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 3299 3896 4683 4951 1239 Size: 393 632 1035 597 787 268 1524 Fragments arranged by size: 1524 1035 787 632 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2048 2048 Size: 5236 EcoRI G'AATT_C Cuts at: 2545 2923 2545 Size: 378 4858 FspI TGC'GCA Cuts at: 3742 3742 Size: 5236 HaeII r_GCGC'y Cuts at: 1678 4383 1678 Size: 2705 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2136 2168 3877 3909 3951 Size: 32 135 32 564 32 1709 32 42 Cuts at: 3951 3983 1373 Size: 32 2626 Fragments arranged by size: 2626 1709 564 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2239 2580 2826 669 Size: 988 281 301 341 246 3079 Fragments arranged by size: 3079 988 341 301 281 246 HindIII A'AGCT_T Cuts at: 748 2205 748 Size: 1457 3779 HpaI GTT'AAC Cuts at: 2826 2826 Size: 5236 KpnI G_GTAC'C Cuts at: 2561 2561 Size: 5236 MfeI C'AATT_G Cuts at: 2835 2835 Size: 5236 MluI A'CGCG_T Cuts at: 5112 5112 Size: 5236 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4350 4534 1305 Size: 3045 184 2007 Fragments arranged by size: 3045 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2084 10 Size: 54 1425 343 252 3162 Fragments arranged by size: 3162 1425 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2436 3194 3553 3712 518 Size: 648 1270 758 359 159 2042 Fragments arranged by size: 2042 1270 758 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2131 2652 3329 4477 4722 1606 Size: 525 521 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 525 521 245 NaeI GCC'GGC Cuts at: 2266 2266 Size: 5236 NcoI C'CATG_G Cuts at: 513 1065 2004 2079 2304 513 Size: 552 939 75 225 3445 Fragments arranged by size: 3445 939 552 225 75 NdeI CA'TA_TG Cuts at: 387 387 Size: 5236 NgoMIV G'CCGG_C Cuts at: 2264 2264 Size: 5236 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5236 NotI GC'GGCC_GC Cuts at: 2683 2683 Size: 5236 NspI r_CATG'y Cuts at: 1132 1229 2284 2594 4139 1132 Size: 97 1055 310 1545 2229 Fragments arranged by size: 2229 1545 1055 310 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5236 PciI A'CATG_T Cuts at: 4135 4135 Size: 5236 PflMI CCAn_nnn'nTGG Cuts at: 1108 2310 1108 Size: 1202 4034 PmeI GTTT'AAAC Cuts at: 2540 2540 Size: 5236 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3305 3337 4818 4850 3305 Size: 32 1481 32 3691 Fragments arranged by size: 3691 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5236 PsiI TTA'TAA Cuts at: 2806 2806 Size: 5236 PstI C_TGCA'G Cuts at: 830 2371 2588 830 Size: 1541 217 3478 Fragments arranged by size: 3478 1541 217 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5236 SalI G'TCGA_C Cuts at: 1936 2578 1936 Size: 642 4594 SbfI CC_TGCA'GG Cuts at: 2588 2588 Size: 5236 ScaI AGT'ACT Cuts at: 3484 5196 3484 Size: 1712 3524 SfcI C'TryA_G Cuts at: 826 1045 2367 2584 3719 4400 4591 826 Size: 219 1322 217 1135 681 191 1471 Fragments arranged by size: 1471 1322 1135 681 219 217 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5236 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5236 SmaI CCC'GGG Cuts at: 1872 2563 1872 Size: 691 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2059 2222 3340 4241 4503 820 Size: 197 926 116 163 1118 901 262 1553 Fragments arranged by size: 1553 1118 926 901 262 197 163 116 SnaBI TAC'GTA Cuts at: 493 3027 493 Size: 2534 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5236 SphI G_CATG'C Cuts at: 1132 2594 1132 Size: 1462 3774 SspI AAT'ATT Cuts at: 5 52 3160 5 Size: 47 3108 2081 Fragments arranged by size: 3108 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5236 StyI C'CwwG_G Cuts at: 513 1065 2004 2079 2304 2675 513 Size: 552 939 75 225 371 3074 Fragments arranged by size: 3074 939 552 371 225 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3263 3280 3433 3618 4063 325 Size: 1255 1683 17 153 185 445 1498 Fragments arranged by size: 1683 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3482 5194 96 Size: 274 80 33 51 1443 1505 1712 138 Fragments arranged by size: 1712 1505 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2377 2409 5196 5228 2377 Size: 32 2787 32 2385 Fragments arranged by size: 2787 2385 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5236 XbaI T'CTAG_A Cuts at: 2572 2572 Size: 5236 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5236 XmaI C'CCGG_G Cuts at: 1870 2561 1870 Size: 691 4545 XmnI GAAnn'nnTTC Cuts at: 2174 2217 3365 2174 Size: 43 1148 4045 Fragments arranged by size: 4045 1148 43 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3044 276 Size: 53 83 186 936 1510 2468 Fragments arranged by size: 2468 1510 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI