################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 439 CCGC CCGC(-3/-1) AcsI 793 AAATTT [GA]^AATT[CT] AfaI 436 GTAC GT^AC ApoI 793 AAATTT [GA]^AATT[CT] BamHI 840 GGATCC G^GATCC BclI 579 TGATCA T^GATCA BlpI 682 GCTCAGC GC^T[ACGT]AGC Bpu1102I 682 GCTCAGC GC^T[ACGT]AGC BsaI 71 GAGACC GGTCTC(1/5) BsaJI 831 CCTGGG C^C[ACGT][ACGT]GG BsmBI 739 GAGACG CGTCTC(1/5) BsmFI 646 GGGAC GGGAC(10/14) CelII 682 GCTCAGC GC^T[ACGT]AGC EarI 52 GAAGAG CTCTTC(1/4) FbaI 579 TGATCA T^GATCA HincII 240 GTTAAC GT[CT]^[GA]AC HindII 240 GTTAAC GT[CT]^[GA]AC HpaI 240 GTTAAC GTT^AAC Ksp632I 52 GAAGAG CTCTTC(1/4) MaeIII 563 GTGAC ^GT[ACGT]AC MspA1I 482 CAGCTG C[AC]G^C[GT]G PflMI 75 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 445 GAGTC GAGTC(4/5) PstI 25 CTGCAG CTGCA^G PvuII 482 CAGCTG CAG^CTG RsaI 436 GTAC GT^AC SfcI 25 CTGCAG C^T[GA][CT]AG StuI 759 AGGCCT AGG^CCT TaqI 735 TCGA T^CGA TfiI 36 GATTC G^A[AT]TC Tsp45I 563 GTGAC ^GT[GC]AC TthHB8I 735 TCGA T^CGA Van91I 75 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwNI 177 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 443 CAGAGTCTG BbvI 608 GCAGC GCAGC(8/12) 24 GCTGC BseRI 55 GAGGAG GAGGAG(10/8) 202 GAGGAG BsmAI 71 GAGAC GTCTC(1/5) 739 GAGAC BsrI 182 ACTGG ACTGG(1/-1) 399 CCAGT BstF5I 490 CATCC GGATG(2/0) 453 GGATG DraI 94 TTTAAA TTT^AAA 598 TTTAAA Fnu4HI 24 GCTGC GC^[ACGT]GC 608 GCAGC FokI 490 CATCC GGATG(9/13) 453 GGATG HaeIII 760 GGCC GG^CC 835 GGCC HinfI 36 GATTC G^A[ACGT]TC 445 GAGTC HphI 744 GGTGA GGTGA(8/7) 41 TCACC ItaI 24 GCTGC GC^[ACGT]GC 608 GCAGC NlaIV 646 GGGACC GG[ACGT]^[ACGT]CC 840 GGATCC SfaNI 386 GATGC GCATC(5/9) 501 GATGC TseI 24 GCTGC G^C[AT]GC 608 GCAGC TspRI 181 CACTG CA[GC]TG[ACGT][ACGT]^ 400 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AluI AlwI AvaII BsiYI BslI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII Sau3AI Sau96I ScrFI Tru9I Tsp509I XhoII