################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaII AviII AvrII BbrPI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MroI MunI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflIII 511 ACGCGT A^C[GA][CT]GT AlwNI 122 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 255 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1023 GGATCC G^GATCC BbvCI 1273 CCTCAGC CCTCAGC(-5/-2) BfaI 656 CTAG C^TAG BglI 472 GCCATCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1016 AGATCT A^GATCT BsmBI 835 GAGACG CGTCTC(1/5) BsmI 970 GAATGC GAATGC(1/-1) BspMI 1037 ACCTGC ACCTGC(4/8) BssSI 214 CTCGTG CACGAG(-5/-1) BstBI 1061 TTCGAA TT^CGAA BstF5I 27 GGATG GGATG(2/0) BstXI 443 CCAGCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 761 AGGCCCT [GA]G^G[ACGT]CC[CT] EarI 660 CTCTTC CTCTTC(1/4) EcoO109I 761 AGGCCCT [GA]G^G[ACGT]CC[CT] FokI 27 GGATG GGATG(9/13) HgaI 510 GACGC GACGC(5/10) Ksp632I 660 CTCTTC CTCTTC(1/4) MaeI 656 CTAG C^TAG MaeII 616 ACGT A^CGT MaeIII 730 GTCAC ^GT[ACGT]AC MluI 511 ACGCGT A^CGCGT MspA1I 774 CCGCTG C[AC]G^C[GT]G NaeI 1113 GCCGGC GCC^GGC NcoI 449 CCATGG C^CATGG NgoMIV 1113 GCCGGC G^CCGGC NspI 68 GCATGT [GA]CATG^[CT] NspV 1061 TTCGAA TT^CGAA PflFI 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 659 GCTCTTC GCTCTTC(1/4) SexAI 296 ACCTGGT A^CC[AT]GGT SfuI 1061 TTCGAA TT^CGAA Tsp45I 730 GTCAC ^GT[GC]AC Tsp509I 1103 AATT ^AATT Tth111I 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 255 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 339 GTAC GT^AC 594 GTAC BanII 421 GGGCTC G[GA]GC[CT]^C 712 GGGCTC BbsI 507 GAAGAC GAAGAC(2/6) 1088 GAAGAC BpuAI 507 GAAGAC GAAGAC(2/6) 1088 GAAGAC BsaI 7 GAGACC GGTCTC(1/5) 884 GGTCTC BseRI 426 CTCCTC GAGGAG(10/8) 1223 CTCCTC BsiEI 323 CGGTCG CG[GA][CT]^CG 830 CGACCG PflMI 121 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1014 CCAGATCTTGG PleI 1091 GACTC GAGTC(4/5) 1159 GAGTC RsaI 339 GTAC GT^AC 594 GTAC SmaI 11 CCCGGG CCC^GGG 610 CCCGGG TfiI 1143 GATTC G^A[AT]TC 1263 GATTC Van91I 121 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1014 CCAGATCTTGG XcmI 755 CCATTAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 842 CCATTCAGCTTATGG XmaCI 11 CCCGGG C^CCGGG 610 CCCGGG XmaI 11 CCCGGG C^CCGGG 610 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AspHI AvaI BalI BanI BbeI BbvI BcgI BcnI BmyI BpmI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfoI StyI TaqI Tru9I TseI TspRI TthHB8I XhoII