(Linear) (Six-base) MAPSORT of: KIEE3527.seq Check: 701 from: 1 to: 1287 KIEE3527.orf With 182 enzymes: SgfI * July 3, 2012 18:05 .. AflIII A'CryG_T Cuts at: 0 511 1287 Size: 511 776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 509 1287 Size: 509 778 AlwNI CAG_nnn'CTG Cuts at: 0 127 1287 Size: 127 1160 AvaI C'yCGr_G Cuts at: 0 11 79 610 1287 Size: 11 68 531 677 Fragments arranged by size: 677 531 68 11 BamHI G'GATC_C Cuts at: 0 1023 1287 Size: 1023 264 BanI G'GyrC_C Cuts at: 0 75 233 480 1133 1287 Size: 75 158 247 653 154 Fragments arranged by size: 653 247 158 154 75 BanII G_rGCy'C Cuts at: 0 425 716 1287 Size: 425 291 571 Fragments arranged by size: 571 425 291 BbeI G_GCGC'C Cuts at: 0 79 237 484 1287 Size: 79 158 247 803 Fragments arranged by size: 803 247 158 79 BbsI GAAGACnn'nnnn_ Cuts at: 0 514 1095 1287 Size: 514 581 192 Fragments arranged by size: 581 514 192 BbvCI CC'TCA_GC Cuts at: 0 1274 1287 Size: 1274 13 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 153 187 378 412 1052 1086 1287 Size: 153 34 191 34 640 34 201 Fragments arranged by size: 640 201 191 153 34 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 478 1287 Size: 478 809 BglII A'GATC_T Cuts at: 0 1016 1287 Size: 1016 271 Bme1580I G_kGCm'C Cuts at: 0 813 1287 Size: 813 474 BmgBI CAC'GTC Cuts at: 0 617 1287 Size: 617 670 BmrI ACTGGGnnnn_n' Cuts at: 0 681 807 1287 Size: 681 126 480 Fragments arranged by size: 681 480 126 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1182 1287 Size: 1182 105 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 1128 1202 1287 Size: 104 1024 74 85 Fragments arranged by size: 1024 104 85 74 Bpu10I CC'TnA_GC Cuts at: 0 1274 1287 Size: 1274 13 BsaI GGTCTCn'nnnn_ Cuts at: 0 1 890 1287 Size: 1 889 397 Fragments arranged by size: 889 397 1 BsaHI Gr'CG_yC Cuts at: 0 76 234 481 1287 Size: 76 158 247 806 Fragments arranged by size: 806 247 158 76 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 102 132 1287 Size: 102 30 1155 Fragments arranged by size: 1155 102 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 417 1214 1287 Size: 417 797 73 Fragments arranged by size: 797 417 73 BseYI C'CCAG_C Cuts at: 0 579 1287 Size: 579 708 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 372 1207 1287 Size: 24 348 835 80 Fragments arranged by size: 835 348 80 24 BsiEI CG_ry'CG Cuts at: 0 326 833 1287 Size: 326 507 454 Fragments arranged by size: 507 454 326 BsiHKAI G_wGCw'C Cuts at: 0 365 557 941 1287 Size: 365 192 384 346 Fragments arranged by size: 384 365 346 192 BsmI GAATG_Cn' Cuts at: 0 976 1287 Size: 976 311 BsmBI CGTCTCn'nnnn_ Cuts at: 0 829 1287 Size: 829 458 Bsp1286I G_dGCh'C Cuts at: 0 365 425 557 716 813 941 1287 Size: 365 60 132 159 97 128 346 Fragments arranged by size: 365 346 159 132 128 97 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1046 1287 Size: 1046 241 BsrBI CCG'CTC Cuts at: 0 462 891 1205 1287 Size: 462 429 314 82 Fragments arranged by size: 462 429 314 82 BsrFI r'CCGG_y Cuts at: 0 230 573 1113 1287 Size: 230 343 540 174 Fragments arranged by size: 540 343 230 174 BssSI C'ACGA_G Cuts at: 0 214 1287 Size: 214 1073 BstBI TT'CG_AA Cuts at: 0 1062 1287 Size: 1062 225 BstXI CCAn_nnnn'nTGG Cuts at: 0 450 1287 Size: 450 837 BstYI r'GATC_y Cuts at: 0 1016 1023 1052 1287 Size: 1016 7 29 235 Fragments arranged by size: 1016 235 29 7 BtgI C'CryG_G Cuts at: 0 152 449 791 1287 Size: 152 297 342 496 Fragments arranged by size: 496 342 297 152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 423 1287 Size: 423 864 BtsI GCAGTG_nn' Cuts at: 0 939 1195 1207 1232 1287 Size: 939 256 12 25 55 Fragments arranged by size: 939 256 55 25 12 EaeI y'GGCC_r Cuts at: 0 203 452 470 548 749 839 1287 Size: 203 249 18 78 201 90 448 Fragments arranged by size: 448 249 203 201 90 78 18 EarI CTCTTCn'nnn_ Cuts at: 0 666 1287 Size: 666 621 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 78 1287 Size: 78 1209 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 1128 1202 1287 Size: 104 1024 74 85 Fragments arranged by size: 1024 104 85 74 EcoO109I rG'GnC_Cy Cuts at: 0 762 1287 Size: 762 525 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 276 1287 Size: 276 1011 HaeII r_GCGC'y Cuts at: 0 79 237 289 449 469 484 724 1287 Size: 79 158 52 160 20 15 240 563 Fragments arranged by size: 563 240 160 158 79 52 20 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 127 159 906 938 1008 1040 1134 1166 Size: 127 32 747 32 70 32 94 32 Cuts at: 1166 1189 1221 1287 Size: 23 32 66 Fragments arranged by size: 747 127 94 70 66 32 32 32 32 32 23 KasI G'GCGC_C Cuts at: 0 75 233 480 1287 Size: 75 158 247 807 Fragments arranged by size: 807 247 158 75 MluI A'CGCG_T Cuts at: 0 511 1287 Size: 511 776 MscI TGG'CCA Cuts at: 0 205 472 751 1287 Size: 205 267 279 536 Fragments arranged by size: 536 279 267 205 MslI CAynn'nnrTG Cuts at: 0 67 334 491 865 1287 Size: 67 267 157 374 422 Fragments arranged by size: 422 374 267 157 67 MspA1I CmG'CkG Cuts at: 0 776 1287 Size: 776 511 NaeI GCC'GGC Cuts at: 0 1115 1287 Size: 1115 172 NarI GG'CG_CC Cuts at: 0 76 234 481 1287 Size: 76 158 247 806 Fragments arranged by size: 806 247 158 76 NcoI C'CATG_G Cuts at: 0 449 1287 Size: 449 838 NgoMIV G'CCGG_C Cuts at: 0 1113 1287 Size: 1113 174 NspI r_CATG'y Cuts at: 0 72 1287 Size: 72 1215 PflMI CCAn_nnn'nTGG Cuts at: 0 127 1020 1287 Size: 127 893 267 Fragments arranged by size: 893 267 127 SapI GCTCTTCn'nnn_ Cuts at: 0 666 1287 Size: 666 621 SexAI A'CCwGG_T Cuts at: 0 296 1287 Size: 296 991 SfoI GGC'GCC Cuts at: 0 77 235 482 1287 Size: 77 158 247 805 Fragments arranged by size: 805 247 158 77 SmaI CCC'GGG Cuts at: 0 13 612 1287 Size: 13 599 675 Fragments arranged by size: 675 599 13 StyI C'CwwG_G Cuts at: 0 449 785 1027 1287 Size: 449 336 242 260 Fragments arranged by size: 449 336 260 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 847 864 1189 1287 Size: 847 17 325 98 Fragments arranged by size: 847 325 98 17 TatI w'GTAC_w Cuts at: 0 593 1287 Size: 593 694 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 102 134 1287 Size: 102 32 1153 Fragments arranged by size: 1153 102 32 Tth111I GACn'n_nGTC Cuts at: 0 298 1287 Size: 298 989 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 762 849 1287 Size: 762 87 438 Fragments arranged by size: 762 438 87 XmaI C'CCGG_G Cuts at: 0 11 610 1287 Size: 11 599 677 Fragments arranged by size: 677 599 11 XmnI GAAnn'nnTTC Cuts at: 0 259 1287 Size: 259 1028 Enzymes that do cut: AflIII AlfI AlwNI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstBI BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I KasI MluI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI SapI SexAI SfoI SmaI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BlpI BmtI BpuEI BsaAI BsaBI BsaWI BsiWI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MmeI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI ZraI