(Circular) (Six-base) MAPSORT of: pFN21AE3521.seq Check: 4823 from: 1 to: 6070 pFN21AE3521 6070 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3880 278 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2440 2607 3413 1937 Size: 503 167 806 4594 Fragments arranged by size: 4594 806 503 167 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3338 4133 5517 5785 1632 Size: 1706 795 1384 268 1917 Fragments arranged by size: 1917 1706 1384 795 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 4969 5946 1203 Size: 3766 977 1327 Fragments arranged by size: 3766 1327 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 4799 4799 Size: 6070 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AlwNI CAG_nnn'CTG Cuts at: 3813 5385 3813 Size: 1572 4498 ApaLI G'TGCA_C Cuts at: 3037 4127 5283 3037 Size: 1090 1156 3824 Fragments arranged by size: 3824 1156 1090 ApoI r'AATT_y Cuts at: 1493 3379 3605 3757 5866 1493 Size: 1886 226 152 2109 1697 Fragments arranged by size: 2109 1886 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 2241 3395 1870 Size: 73 298 1154 4545 Fragments arranged by size: 4545 1154 298 73 AvrII C'CTAG_G Cuts at: 2038 2038 Size: 6070 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2711 2744 2711 Size: 33 6037 BamHI G'GATC_C Cuts at: 3400 3767 3400 Size: 367 5703 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2678 3391 4846 618 Size: 325 217 627 255 636 713 1455 1842 Fragments arranged by size: 1842 1455 713 636 627 325 255 217 BanII G_rGCy'C Cuts at: 1391 2099 2286 2345 3389 1391 Size: 708 187 59 1044 4072 Fragments arranged by size: 4072 1044 708 187 59 BbeI G_GCGC'C Cuts at: 2046 2046 Size: 6070 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5099 BbvCI CC'TCA_GC Cuts at: 3340 3340 Size: 6070 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4261 4295 1903 Size: 34 50 34 2240 34 3678 Fragments arranged by size: 3678 2240 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2313 3962 5172 2313 Size: 1649 1210 3211 Fragments arranged by size: 3211 1649 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6070 BfrBI ATG'CAT Cuts at: 2990 2990 Size: 6070 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4681 136 Size: 107 122 71 631 605 3009 1525 Fragments arranged by size: 3009 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6065 6065 Size: 6070 BlpI GC'TnA_GC Cuts at: 3487 3487 Size: 6070 Bme1580I G_kGCm'C Cuts at: 1790 3041 4131 5287 1790 Size: 1251 1090 1156 2573 Fragments arranged by size: 2573 1251 1156 1090 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3801 4759 442 Size: 416 53 83 399 24 2384 958 1753 Fragments arranged by size: 2384 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2113 2113 Size: 6070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2375 2417 2628 4730 1239 Size: 1136 42 211 2102 2579 Fragments arranged by size: 2579 2102 1136 211 42 Bpu10I CC'TnA_GC Cuts at: 3340 3340 Size: 6070 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2435 4195 5060 5358 2435 Size: 1760 865 298 3147 Fragments arranged by size: 3147 1760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3227 4733 882 Size: 617 1728 1506 2219 Fragments arranged by size: 2219 1728 1506 617 BsaAI yAC'GTr Cuts at: 493 1204 2550 3861 493 Size: 711 1346 1311 2702 Fragments arranged by size: 2702 1346 1311 711 BsaBI GATnn'nnATC Cuts at: 6064 6064 Size: 6070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 3877 4259 4894 Size: 53 83 186 936 510 1834 382 635 Cuts at: 4894 275 Size: 1451 Fragments arranged by size: 1834 1451 936 635 510 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4503 4901 5175 5322 5733 4503 Size: 398 274 147 411 4840 Fragments arranged by size: 4840 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2195 1178 Size: 30 165 30 762 30 5053 Fragments arranged by size: 5053 762 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 2102 2170 1186 Size: 912 4 68 5086 Fragments arranged by size: 5086 912 68 4 BseYI C'CCAG_C Cuts at: 5273 5769 5273 Size: 496 5574 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2528 1163 Size: 573 792 4705 Fragments arranged by size: 4705 792 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3520 4281 5309 1540 Size: 275 186 1519 761 1028 2301 Fragments arranged by size: 2301 1519 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2099 3041 4131 4216 5287 2099 Size: 942 1090 85 1071 2882 Fragments arranged by size: 2882 1090 1071 942 85 BsmI GAATG_Cn' Cuts at: 2847 2990 3581 3674 2847 Size: 143 591 93 5243 Fragments arranged by size: 5243 591 143 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2148 2148 Size: 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 2286 2345 3041 3389 4131 4216 Size: 399 309 187 59 696 348 742 85 Cuts at: 4216 5287 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 696 399 348 309 187 85 59 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 2322 3959 5689 2322 Size: 1637 1730 2703 Fragments arranged by size: 2703 1730 1637 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3425 844 Size: 2581 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3957 1394 Size: 429 2134 3507 Fragments arranged by size: 3507 2134 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2563 4565 4739 65 Size: 1183 1315 2002 174 1396 Fragments arranged by size: 2002 1396 1315 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2473 4714 4901 1700 Size: 110 663 2241 187 2869 Fragments arranged by size: 2869 2241 663 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6070 BssSI C'ACGA_G Cuts at: 4130 5142 4130 Size: 1012 5058 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6070 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6070 BstXI CCAn_nnnn'nTGG Cuts at: 3074 3074 Size: 6070 BstYI r'GATC_y Cuts at: 1972 3069 3400 3767 4152 4169 5621 5707 5719 Size: 1097 331 367 385 17 1452 86 12 Cuts at: 5719 6065 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1097 385 367 346 331 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2627 513 Size: 552 1562 3956 Fragments arranged by size: 3956 1562 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5331 Fragments arranged by size: 5331 683 56 BtsI GCAGTG_nn' Cuts at: 2405 3586 4380 4400 2405 Size: 1181 794 20 4075 Fragments arranged by size: 4075 1181 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 3374 3374 Size: 6070 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5077 809 Size: 507 3761 1802 Fragments arranged by size: 3761 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 2402 2859 Size: 54 1425 50 275 18 432 140 457 Cuts at: 2859 3057 3517 4406 6035 8 Size: 198 460 889 1629 43 Fragments arranged by size: 1629 1425 889 460 457 432 275 198 140 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3517 1537 Size: 275 1705 4090 Fragments arranged by size: 4090 1705 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 3099 3200 3357 4000 1353 Size: 734 1012 101 157 643 3423 Fragments arranged by size: 3423 1012 734 643 157 101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2199 2210 3094 4657 5031 5177 Size: 1064 138 610 11 884 1563 374 146 Cuts at: 5177 387 Size: 1280 Fragments arranged by size: 1563 1280 1064 884 610 374 146 138 11 EcoICRI GAG'CTC Cuts at: 2097 2097 Size: 6070 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2375 2417 2628 3338 4133 4730 5517 Size: 393 743 42 211 710 795 597 787 Cuts at: 5517 5785 1239 Size: 268 1524 Fragments arranged by size: 1524 795 787 743 710 597 393 268 211 42 EcoRI G'AATT_C Cuts at: 3379 3757 3379 Size: 378 5692 FspI TGC'GCA Cuts at: 4576 4576 Size: 6070 HaeII r_GCGC'y Cuts at: 1678 2046 5217 1678 Size: 368 3171 2531 Fragments arranged by size: 3171 2531 368 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2123 2155 2433 2447 2465 Size: 32 135 32 551 32 278 14 18 Cuts at: 2465 2479 2894 2926 3009 3041 4711 4743 4785 Size: 14 415 32 83 32 1670 32 42 Cuts at: 4785 4817 1373 Size: 32 2626 Fragments arranged by size: 2626 1670 551 415 278 135 83 42 32 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 2720 3414 3660 669 Size: 988 281 782 694 246 3079 Fragments arranged by size: 3079 988 782 694 281 246 HindIII A'AGCT_T Cuts at: 748 3363 748 Size: 2615 3455 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KasI G'GCGC_C Cuts at: 2042 2042 Size: 6070 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5184 5368 1305 Size: 3879 184 2007 Fragments arranged by size: 3879 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2404 10 Size: 54 1425 343 572 3676 Fragments arranged by size: 3676 1425 572 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4028 4387 4546 518 Size: 648 2862 359 159 2042 Fragments arranged by size: 2862 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 3335 3486 4163 5311 5556 1606 Size: 408 1321 151 677 1148 245 2120 Fragments arranged by size: 2120 1321 1148 677 408 245 151 NarI GG'CG_CC Cuts at: 2043 2043 Size: 6070 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5518 NdeI CA'TA_TG Cuts at: 387 2811 3153 387 Size: 2424 342 3304 Fragments arranged by size: 3304 2424 342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NsiI A_TGCA'T Cuts at: 2992 2992 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 3092 3428 4973 1132 Size: 97 1863 336 1545 2229 Fragments arranged by size: 2229 1863 1545 336 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6070 PciI A'CATG_T Cuts at: 4969 4969 Size: 6070 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6070 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6070 PsiI TTA'TAA Cuts at: 3640 3640 Size: 6070 PstI C_TGCA'G Cuts at: 830 3422 830 Size: 2592 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 PvuII CAG'CTG Cuts at: 2014 3335 2014 Size: 1321 4749 SacI G_AGCT'C Cuts at: 2099 2099 Size: 6070 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SapI GCTCTTCn'nnn_ Cuts at: 2087 3099 2087 Size: 1012 5058 SbfI CC_TGCA'GG Cuts at: 3422 3422 Size: 6070 ScaI AGT'ACT Cuts at: 4318 6030 4318 Size: 1712 4358 SfcI C'TryA_G Cuts at: 826 1045 3418 4553 5234 5425 826 Size: 219 2373 1135 681 191 1471 Fragments arranged by size: 2373 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2044 2044 Size: 6070 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 2243 3397 1872 Size: 371 1154 4545 Fragments arranged by size: 4545 1154 371 SmlI C'TyrA_G Cuts at: 820 1017 1943 2450 4174 5075 5337 820 Size: 197 926 507 1724 901 262 1553 Fragments arranged by size: 1724 1553 926 901 507 262 197 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3092 3428 1132 Size: 1960 336 3774 Fragments arranged by size: 3774 1960 336 SspI AAT'ATT Cuts at: 5 52 3994 5 Size: 47 3942 2081 Fragments arranged by size: 3942 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6070 StyI C'CwwG_G Cuts at: 513 1065 2038 3073 3509 513 Size: 552 973 1035 436 3074 Fragments arranged by size: 3074 1035 973 552 436 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4097 4114 4267 4452 4897 325 Size: 1255 2517 17 153 185 445 1498 Fragments arranged by size: 2517 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4316 6028 96 Size: 274 80 33 51 1443 2339 1712 138 Fragments arranged by size: 2339 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6030 6062 6030 Size: 32 6038 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 2241 3395 1870 Size: 371 1154 4545 Fragments arranged by size: 4545 1154 371 XmnI GAAnn'nnTTC Cuts at: 4199 4199 Size: 6070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3878 276 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI