(Circular) (Six-base) MAPSORT of: pFN21AB8505.seq Check: 3062 from: 1 to: 7456 pFN21AB8505 7456 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5266 278 Size: 53 83 186 936 3730 2468 Fragments arranged by size: 3730 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4799 1937 Size: 2862 4594 Acc65I G'GTAC_C Cuts at: 4777 4777 Size: 7456 AclI AA'CG_TT Cuts at: 1563 3025 3405 5583 5956 1563 Size: 1462 380 2178 373 3063 Fragments arranged by size: 3063 2178 1462 380 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2068 2461 3744 5519 6903 7171 1632 Size: 436 393 1283 1775 1384 268 1917 Fragments arranged by size: 1917 1775 1384 1283 436 393 268 AflII C'TTAA_G Cuts at: 820 1017 3811 820 Size: 197 2794 4465 Fragments arranged by size: 4465 2794 197 AflIII A'CryG_T Cuts at: 1203 3980 6355 7332 1203 Size: 2777 2375 977 1327 Fragments arranged by size: 2777 2375 1327 977 AgeI A'CCGG_T Cuts at: 6287 6287 Size: 7456 AhdI GACnn_n'nnGTC Cuts at: 2592 6185 2592 Size: 3593 3863 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7456 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4403 1184 Size: 3219 4237 AlwNI CAG_nnn'CTG Cuts at: 3383 5199 6771 3383 Size: 1816 1572 4068 Fragments arranged by size: 4068 1816 1572 ApaI G_GGCC'C Cuts at: 4718 4718 Size: 7456 ApaLI G'TGCA_C Cuts at: 4065 5513 6669 4065 Size: 1448 1156 4852 Fragments arranged by size: 4852 1448 1156 ApoI r'AATT_y Cuts at: 1493 2344 3340 4765 4991 5143 7252 1493 Size: 851 996 1425 226 152 2109 1697 Fragments arranged by size: 2109 1697 1425 996 851 226 152 AseI AT'TA_AT Cuts at: 160 1032 2411 6010 160 Size: 872 1379 3599 1606 Fragments arranged by size: 3599 1606 1379 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 AvaI C'yCGr_G Cuts at: 1870 1943 4781 1870 Size: 73 2838 4545 Fragments arranged by size: 4545 2838 73 AvrII C'CTAG_G Cuts at: 3398 3398 Size: 7456 BamHI G'GATC_C Cuts at: 4786 5153 4786 Size: 367 7089 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4777 6232 618 Size: 325 217 627 2990 1455 1842 Fragments arranged by size: 2990 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2426 4440 4718 4775 1391 Size: 1035 2014 278 57 4072 Fragments arranged by size: 4072 2014 1035 278 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2588 3157 3651 4479 928 Size: 971 689 569 494 828 3905 Fragments arranged by size: 3905 971 828 689 569 494 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5647 5681 1903 Size: 34 3710 34 3678 Fragments arranged by size: 3710 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5348 6558 5348 Size: 1210 6246 BclI T'GATC_A Cuts at: 1548 3952 1548 Size: 2404 5052 BfrBI ATG'CAT Cuts at: 4586 4586 Size: 7456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3368 6067 136 Size: 107 122 71 631 605 1696 2699 1525 Fragments arranged by size: 2699 1696 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7451 7451 Size: 7456 BlpI GC'TnA_GC Cuts at: 2669 4873 2669 Size: 2204 5252 Bme1580I G_kGCm'C Cuts at: 1790 2176 4069 4718 5517 6673 1790 Size: 386 1893 649 799 1156 2573 Fragments arranged by size: 2573 1893 1156 799 649 386 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7456 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 2721 3568 Size: 416 53 83 399 24 762 542 847 Cuts at: 3568 3931 4712 5187 6145 442 Size: 363 781 475 958 1753 Fragments arranged by size: 1753 958 847 781 762 542 475 416 399 363 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7456 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3469 3469 Size: 7456 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2411 4762 6116 1239 Size: 1172 2351 1354 2579 Fragments arranged by size: 2579 2351 1354 1172 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2499 4091 4298 5581 6446 6744 2499 Size: 1592 207 1283 865 298 3211 Fragments arranged by size: 3211 1592 1283 865 298 207 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6119 882 Size: 617 4620 2219 Fragments arranged by size: 4620 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5247 493 Size: 711 4043 2702 Fragments arranged by size: 4043 2702 711 BsaBI GATnn'nnATC Cuts at: 3522 7450 3522 Size: 3928 3528 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4424 5263 5645 6280 Size: 53 83 186 936 2891 839 382 635 Cuts at: 6280 275 Size: 1451 Fragments arranged by size: 2891 1451 936 839 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4553 5889 6287 6561 6708 7119 4553 Size: 1336 398 274 147 411 4890 Fragments arranged by size: 4890 1336 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4622 4652 1178 Size: 30 165 30 3219 30 3982 Fragments arranged by size: 3982 3219 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2202 4088 1186 Size: 1016 1886 4554 Fragments arranged by size: 4554 1886 1016 BseYI C'CCAG_C Cuts at: 6659 7155 6659 Size: 496 6960 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4275 1163 Size: 573 2539 4344 Fragments arranged by size: 4344 2539 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4906 5667 6695 1540 Size: 275 186 2905 761 1028 2301 Fragments arranged by size: 2905 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2426 4069 4440 5517 5602 6673 2426 Size: 1643 371 1077 85 1071 3209 Fragments arranged by size: 3209 1643 1077 1071 371 85 BsmI GAATG_Cn' Cuts at: 4967 5060 4967 Size: 93 7363 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2176 2426 4069 4440 4718 4775 5517 Size: 399 386 250 1643 371 278 57 742 Cuts at: 5517 5602 6673 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1643 1071 742 399 386 371 278 250 85 57 BspEI T'CCGG_A Cuts at: 7119 7119 Size: 7456 BspHI T'CATG_A Cuts at: 2977 3019 4726 5345 7075 2977 Size: 42 1707 619 1730 3358 Fragments arranged by size: 3358 1730 1707 619 42 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4811 844 Size: 3967 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5343 1394 Size: 429 3520 3507 Fragments arranged by size: 3520 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2558 2955 3884 4096 4284 5951 6125 Size: 1183 1310 397 929 212 188 1667 174 Cuts at: 6125 65 Size: 1396 Fragments arranged by size: 1667 1396 1310 1183 929 397 212 188 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6100 6287 1700 Size: 110 4290 187 2869 Fragments arranged by size: 4290 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7456 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7456 BssSI C'ACGA_G Cuts at: 5516 6528 5516 Size: 1012 6444 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7456 BstBI TT'CG_AA Cuts at: 3850 6260 3850 Size: 2410 5046 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7456 BstYI r'GATC_y Cuts at: 1972 3292 3889 4077 4786 5153 5538 5555 7007 Size: 1320 597 188 709 367 385 17 1452 Cuts at: 7007 7093 7105 7451 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1320 709 597 385 367 346 188 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2430 2430 Size: 7456 BtgI C'CryG_G Cuts at: 513 1065 2004 2445 513 Size: 552 939 441 5524 Fragments arranged by size: 5524 939 552 441 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6717 Fragments arranged by size: 6717 683 56 BtsI GCAGTG_nn' Cuts at: 3378 4972 5766 5786 3378 Size: 1594 794 20 5048 Fragments arranged by size: 5048 1594 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7456 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7421 DraI TTT'AAA Cuts at: 2014 4760 2014 Size: 2746 4710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6463 809 Size: 507 5147 1802 Fragments arranged by size: 5147 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 4903 5792 Size: 54 1425 50 275 18 552 2521 889 Cuts at: 5792 7421 8 Size: 1629 43 Fragments arranged by size: 2521 1629 1425 889 552 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4903 1537 Size: 275 3091 4090 Fragments arranged by size: 4090 3091 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2202 3501 5386 1353 Size: 734 115 1299 1885 3423 Fragments arranged by size: 3423 1885 1299 734 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6043 6417 6563 387 Size: 1064 138 4454 374 146 1280 Fragments arranged by size: 4454 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2424 4438 2424 Size: 2014 5442 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2411 2461 3744 4762 5519 6116 Size: 393 436 343 50 1283 1018 757 597 Cuts at: 6116 6903 7171 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1283 1018 787 757 597 436 393 343 268 50 EcoO109I rG'GnC_Cy Cuts at: 4122 4714 4122 Size: 592 6864 EcoRI G'AATT_C Cuts at: 4765 5143 4765 Size: 378 7078 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2915 3119 2915 Size: 204 7252 FspI TGC'GCA Cuts at: 5962 5962 Size: 7456 HaeII r_GCGC'y Cuts at: 1678 6603 1678 Size: 4925 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2578 2610 2690 2722 3494 Size: 32 135 32 1006 32 80 32 772 Cuts at: 3494 3526 4409 4441 6097 6129 6171 6203 1373 Size: 32 883 32 1656 32 42 32 2626 Fragments arranged by size: 2626 1656 1006 883 772 135 80 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4402 4800 5046 669 Size: 988 281 2464 398 246 3079 Fragments arranged by size: 3079 2464 988 398 281 246 HindIII A'AGCT_T Cuts at: 748 3615 4026 4207 748 Size: 2867 411 181 3997 Fragments arranged by size: 3997 2867 411 181 HpaI GTT'AAC Cuts at: 4402 5046 4402 Size: 644 6812 KpnI G_GTAC'C Cuts at: 4781 4781 Size: 7456 MfeI C'AATT_G Cuts at: 2070 4263 4359 5055 2070 Size: 2193 96 696 4471 Fragments arranged by size: 4471 2193 696 96 MluI A'CGCG_T Cuts at: 7332 7332 Size: 7456 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 6570 6754 1305 Size: 1008 4257 184 2007 Fragments arranged by size: 4257 2007 1008 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 10 Size: 54 1425 343 552 5082 Fragments arranged by size: 5082 1425 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2539 4731 5414 5773 5932 518 Size: 648 1373 2192 683 359 159 2042 Fragments arranged by size: 2192 2042 1373 683 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3668 4872 5549 6697 6942 1606 Size: 2062 1204 677 1148 245 2120 Fragments arranged by size: 2120 2062 1204 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2445 513 Size: 552 939 441 5524 Fragments arranged by size: 5524 939 552 441 NdeI CA'TA_TG Cuts at: 387 387 Size: 7456 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7456 NotI GC'GGCC_GC Cuts at: 4903 4903 Size: 7456 NsiI A_TGCA'T Cuts at: 4588 4588 Size: 7456 NspI r_CATG'y Cuts at: 1132 1229 2935 3079 3984 4089 4814 6359 1132 Size: 97 1706 144 905 105 725 1545 2229 Fragments arranged by size: 2229 1706 1545 905 725 144 105 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7456 PciI A'CATG_T Cuts at: 3980 6355 3980 Size: 2375 5081 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7456 PfoI T'CCnGG_A Cuts at: 4212 4544 4212 Size: 332 7124 PmeI GTTT'AAAC Cuts at: 4760 4760 Size: 7456 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5525 5557 7038 7070 5525 Size: 32 1481 32 5911 Fragments arranged by size: 5911 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4122 4122 Size: 7456 PshAI GACnn'nnGTC Cuts at: 1613 2946 1613 Size: 1333 6123 PsiI TTA'TAA Cuts at: 3927 5026 3927 Size: 1099 6357 PspOMI G'GGCC_C Cuts at: 4714 4714 Size: 7456 PstI C_TGCA'G Cuts at: 830 3315 4808 830 Size: 2485 1493 3478 Fragments arranged by size: 3478 2485 1493 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7456 PvuII CAG'CTG Cuts at: 3668 3668 Size: 7456 SacI G_AGCT'C Cuts at: 2426 4440 2426 Size: 2014 5442 SalI G'TCGA_C Cuts at: 1936 4798 1936 Size: 2862 4594 SapI GCTCTTCn'nnn_ Cuts at: 2202 2202 Size: 7456 SbfI CC_TGCA'GG Cuts at: 4808 4808 Size: 7456 ScaI AGT'ACT Cuts at: 4560 5704 7416 4560 Size: 1144 1712 4600 Fragments arranged by size: 4600 1712 1144 SfcI C'TryA_G Cuts at: 826 1045 3311 4804 5939 6620 6811 826 Size: 219 2266 1493 1135 681 191 1471 Fragments arranged by size: 2266 1493 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7456 SmaI CCC'GGG Cuts at: 1872 4783 1872 Size: 2911 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2514 3811 4070 4313 5560 6461 Size: 197 926 571 1297 259 243 1247 901 Cuts at: 6461 6723 820 Size: 262 1553 Fragments arranged by size: 1553 1297 1247 926 901 571 262 259 243 197 SnaBI TAC'GTA Cuts at: 493 5247 493 Size: 4754 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7456 SphI G_CATG'C Cuts at: 1132 4814 1132 Size: 3682 3774 SspI AAT'ATT Cuts at: 5 52 2783 3046 3626 5380 5 Size: 47 2731 263 580 1754 2081 Fragments arranged by size: 2731 2081 1754 580 263 47 StuI AGG'CCT Cuts at: 1517 4319 1517 Size: 2802 4654 StyI C'CwwG_G Cuts at: 513 1065 2004 2445 3108 3398 4895 513 Size: 552 939 441 663 290 1497 3074 Fragments arranged by size: 3074 1497 939 663 552 441 290 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5483 5500 5653 5838 6283 325 Size: 1255 3903 17 153 185 445 1498 Fragments arranged by size: 3903 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2545 4558 5702 Size: 274 80 33 51 1443 568 2013 1144 Cuts at: 5702 7414 96 Size: 1712 138 Fragments arranged by size: 2013 1712 1443 1144 568 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4208 4240 4622 4654 7416 7448 4208 Size: 32 382 32 2762 32 4216 Fragments arranged by size: 4216 2762 382 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7456 XbaI T'CTAG_A Cuts at: 3583 4009 4792 3583 Size: 426 783 6247 Fragments arranged by size: 6247 783 426 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2758 3370 2758 Size: 612 6844 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7456 XmaI C'CCGG_G Cuts at: 1870 4781 1870 Size: 2911 4545 XmnI GAAnn'nnTTC Cuts at: 2367 3099 3407 3657 4027 5585 2367 Size: 732 308 250 370 1558 4238 Fragments arranged by size: 4238 1558 732 370 308 250 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5264 276 Size: 53 83 186 936 3730 2468 Fragments arranged by size: 3730 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI