################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AccIII AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BlnI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KspI MfeI MluI MroI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NotI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 640 GGTACC G^GTACC AclI 1590 AACGTT AA^CGTT AcsI 1120 GAATTT [GA]^AATT[CT] AlwNI 1727 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 870 CCTGAGG CC^T[ACGT]AGG ApaI 1720 GGGCCC GGGCC^C ApoI 1120 GAATTT [GA]^AATT[CT] Asp700I 1656 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 640 GGTACC G^GTACC AspI 254 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 611 TGGCCA TGG^CCA BanI 640 GGTACC G^G[CT][GA]CC BbvCI 1566 CCTCAGC CCTCAGC(-5/-2) BglI 1325 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 803 AGATCT A^GATCT BlpI 1263 GCTGAGC GC^T[ACGT]AGC BpmI 284 CTGGAG CTGGAG(16/14) Bpu1102I 1263 GCTGAGC GC^T[ACGT]AGC BsaAI 451 TACGTG [CT]AC^GT[GA] BsaBI 108 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 237 GAGACG CGTCTC(1/5) BstAPI 741 GCAGATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 870 CCTGAGG CC^T[ACGT]AGG CelII 1263 GCTGAGC GC^T[ACGT]AGC DrdI 239 GACGCATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 399 CCACGG C^C[GA][CT]GG Eco81I 870 CCTGAGG CC^T[ACGT]AGG HgaI 239 GACGC GACGC(5/10) KpnI 640 GGTACC GGTAC^C MamI 108 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 611 TGGCCA TGG^CCA MscI 611 TGGCCA TGG^CCA NheI 393 GCTAGC G^CTAGC NruI 996 TCGCGA TCG^CGA NspI 1682 ACATGC [GA]CATG^[CT] PflFI 254 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1251 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1590 AACGTT AA^CGTT PvuII 862 CAGCTG CAG^CTG SacI 1272 GAGCTC GAGCT^C SmaI 1534 CCCGGG CCC^GGG StuI 1550 AGGCCT AGG^CCT TfiI 1130 GAATC G^A[AT]TC Tsp509I 1121 AATT ^AATT Tth111I 254 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1251 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1478 CCAGCAGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1534 CCCGGG C^CCGGG XmaI 1534 CCCGGG C^CCGGG XmnI 1656 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 641 GTAC GT^AC 1563 GTAC AvaI 617 CTCGGG C^[CT]CG[GA]G 1534 CCCGGG BbsI 436 GTCTTC GAAGAC(2/6) 703 GTCTTC BpuAI 436 GTCTTC GAAGAC(2/6) 703 GTCTTC BsoBI 617 CTCGGG C^[CT]CG[GA]G 1534 CCCGGG BsrI 117 CCAGT ACTGG(1/-1) 163 CCAGT BstXI 342 CCAGGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1478 CCAGCAGGGTGG CfoI 264 GCGC GCG^C 903 GCGC DraIII 208 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1230 CACCCTGTG EcoNI 786 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 870 CCTGAGGAAGG HhaI 264 GCGC GCG^C 903 GCGC HinP1I 264 GCGC G^CGC 903 GCGC RsaI 641 GTAC GT^AC 1563 GTAC TspRI 508 CAGTG CA[GC]TG[ACGT][ACGT]^ 1061 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AluI AlwI AspHI AvaII BanII BbvI BcnI BfaI BmyI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I XhoII