(Circular) (Six-base) MAPSORT of: pFN21AE3530.seq Check: 5803 from: 1 to: 8425 pFN21AE3530 8425 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2818 3202 3703 4704 5698 2818 Size: 384 501 1001 994 5545 Fragments arranged by size: 5545 1001 994 501 384 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2406 2718 5544 6235 Size: 53 83 186 936 870 312 2826 691 Cuts at: 6235 278 Size: 2468 Fragments arranged by size: 2826 2468 936 870 691 312 186 83 53 AccI GT'mk_AC Cuts at: 1937 5768 1937 Size: 3831 4594 Acc65I G'GTAC_C Cuts at: 5746 5746 Size: 8425 AclI AA'CG_TT Cuts at: 1563 6552 6925 1563 Size: 4989 373 3063 Fragments arranged by size: 4989 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2912 3278 3326 6488 7872 8140 1632 Size: 1280 366 48 3162 1384 268 1917 Fragments arranged by size: 3162 1917 1384 1280 366 268 48 AfeI AGC'GCT Cuts at: 5644 5644 Size: 8425 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8228 AflIII A'CryG_T Cuts at: 1203 7324 8301 1203 Size: 6121 977 1327 Fragments arranged by size: 6121 1327 977 AgeI A'CCGG_T Cuts at: 2358 7256 2358 Size: 4898 3527 AhdI GACnn_n'nnGTC Cuts at: 3337 7154 3337 Size: 3817 4608 AleI CACnn'nnGTG Cuts at: 1166 2261 5085 1166 Size: 1095 2824 4506 Fragments arranged by size: 4506 2824 1095 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2279 3674 5420 1184 Size: 1095 1395 1746 4189 Fragments arranged by size: 4189 1746 1395 1095 AlwNI CAG_nnn'CTG Cuts at: 2243 3433 3853 3862 3934 3979 4466 4538 4633 Size: 1190 420 9 72 45 487 72 95 Cuts at: 4633 5116 6168 7740 2243 Size: 483 1052 1572 2928 Fragments arranged by size: 2928 1572 1190 1052 487 483 420 95 72 72 45 9 ApaI G_GGCC'C Cuts at: 2317 2485 2801 3672 4291 4800 4857 5310 5444 Size: 168 316 871 619 509 57 453 134 Cuts at: 5444 2317 Size: 5298 Fragments arranged by size: 5298 871 619 509 453 316 168 134 57 ApaLI G'TGCA_C Cuts at: 6482 7638 6482 Size: 1156 7269 ApoI r'AATT_y Cuts at: 1493 5734 5960 6112 8221 1493 Size: 4241 226 152 2109 1697 Fragments arranged by size: 4241 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6979 160 Size: 872 5947 1606 Fragments arranged by size: 5947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8425 AvaI C'yCGr_G Cuts at: 1870 1943 2435 2545 3191 4263 4574 4799 4857 Size: 73 492 110 646 1072 311 225 58 Cuts at: 4857 5310 5437 5750 1870 Size: 453 127 313 4545 Fragments arranged by size: 4545 1072 646 492 453 313 311 225 127 110 73 58 AvrII C'CTAG_G Cuts at: 2503 2503 Size: 8425 BamHI G'GATC_C Cuts at: 5755 6122 5755 Size: 367 8058 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2263 3658 3740 3779 3920 Size: 325 217 627 476 1395 82 39 141 Cuts at: 3920 3960 4224 4622 4985 5467 5710 5746 7201 Size: 40 264 398 363 482 243 36 1455 Cuts at: 7201 618 Size: 1842 Fragments arranged by size: 1842 1455 1395 627 482 476 398 363 325 264 243 217 141 82 40 39 36 BanII G_rGCy'C Cuts at: 1391 2317 2420 2485 2516 2583 2751 2801 2822 Size: 926 103 65 31 67 168 50 21 Cuts at: 2822 2855 2940 3672 4291 4529 4581 4652 4775 Size: 33 85 732 619 238 52 71 123 Cuts at: 4775 4800 4857 5310 5352 5444 5744 1391 Size: 25 57 453 42 92 300 4072 Fragments arranged by size: 4072 926 732 619 453 300 238 168 123 103 92 85 71 67 65 57 52 50 42 33 31 25 21 BbeI G_GCGC'C Cuts at: 3783 5471 3783 Size: 1688 6737 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2672 3565 928 Size: 971 773 893 5788 Fragments arranged by size: 5788 971 893 773 BbvCI CC'TCA_GC Cuts at: 2823 4893 5301 5316 2823 Size: 2070 408 15 5932 Fragments arranged by size: 5932 2070 408 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6616 6650 1903 Size: 34 4679 34 3678 Fragments arranged by size: 4679 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2892 6317 7527 2892 Size: 3425 1210 3790 Fragments arranged by size: 3790 3425 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3667 3788 4180 Size: 107 122 71 631 605 1995 121 392 Cuts at: 4180 4841 5243 5335 5370 7036 136 Size: 661 402 92 35 1666 1525 Fragments arranged by size: 1995 1666 1525 661 631 605 402 392 122 121 107 92 71 35 BglII A'GATC_T Cuts at: 8420 8420 Size: 8425 BlpI GC'TnA_GC Cuts at: 2948 5842 2948 Size: 2894 5531 Bme1580I G_kGCm'C Cuts at: 1790 2317 2485 2801 3663 3672 4229 4270 4291 Size: 527 168 316 862 9 557 41 21 Cuts at: 4291 4800 4857 5132 5223 5310 5444 5715 6486 Size: 509 57 275 91 87 134 271 771 Cuts at: 6486 7642 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 862 771 557 527 509 316 275 271 168 134 91 87 57 41 21 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8425 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4621 6156 7114 Size: 416 53 83 399 24 3204 1535 958 Cuts at: 7114 442 Size: 1753 Fragments arranged by size: 3204 1753 1535 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8425 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4024 5527 5611 4024 Size: 1503 84 6838 Fragments arranged by size: 6838 1503 84 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2308 2500 2530 2681 2774 2888 3386 3662 Size: 1069 192 30 151 93 114 498 276 Cuts at: 3662 4517 4565 4787 4847 5039 7085 1239 Size: 855 48 222 60 192 2046 2579 Fragments arranged by size: 2579 2046 1069 855 498 276 222 192 192 151 114 93 60 48 30 Bpu10I CC'TnA_GC Cuts at: 2816 2823 4109 4893 5301 5316 2816 Size: 7 1286 784 408 15 5925 Fragments arranged by size: 5925 1286 784 408 15 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3185 3936 6550 7415 7713 3185 Size: 751 2614 865 298 3897 Fragments arranged by size: 3897 2614 865 751 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3515 5006 5212 7088 882 Size: 617 2016 1491 206 1876 2219 Fragments arranged by size: 2219 2016 1876 1491 617 206 BsaAI yAC'GTr Cuts at: 493 1204 2881 3127 5368 6216 493 Size: 711 1677 246 2241 848 2702 Fragments arranged by size: 2702 2241 1677 848 711 246 BsaBI GATnn'nnATC Cuts at: 2427 5039 8419 2427 Size: 2612 3380 2433 Fragments arranged by size: 3380 2612 2433 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2157 2403 2715 3780 Size: 53 83 186 936 624 246 312 1065 Cuts at: 3780 4962 5468 5541 5574 6232 6614 7249 275 Size: 1182 506 73 33 658 382 635 1451 Fragments arranged by size: 1451 1182 1065 936 658 635 624 506 382 312 246 186 83 73 53 33 BsaWI w'CCGG_w Cuts at: 2358 2835 3328 6858 7256 7530 7677 8088 2358 Size: 477 493 3530 398 274 147 411 2695 Fragments arranged by size: 3530 2695 493 477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2195 2225 2503 2533 2990 Size: 30 165 30 792 30 278 30 457 Cuts at: 2990 3020 3512 3542 4639 4669 1178 Size: 30 492 30 1097 30 4934 Fragments arranged by size: 4934 1097 792 492 457 278 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2013 2065 2567 2747 2750 4178 4249 4514 Size: 827 52 502 180 3 1428 71 265 Cuts at: 4514 4517 4589 4969 5309 5333 5516 5687 1186 Size: 3 72 380 340 24 183 171 3924 Fragments arranged by size: 3924 1428 827 502 380 340 265 183 180 171 72 71 52 24 3 3 BseYI C'CCAG_C Cuts at: 2162 2236 2477 2619 2782 2794 4321 7628 8124 Size: 74 241 142 163 12 1527 3307 496 Cuts at: 8124 2162 Size: 2463 Fragments arranged by size: 3307 2463 1527 496 241 163 142 74 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2687 2882 2981 3238 3731 3899 1163 Size: 573 951 195 99 257 493 168 5689 Fragments arranged by size: 5689 951 573 493 257 195 168 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4066 5875 6636 7664 1540 Size: 275 186 2065 1809 761 1028 2301 Fragments arranged by size: 2301 2065 1809 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2516 3765 4473 4671 5496 6486 6571 7642 2516 Size: 1249 708 198 825 990 85 1071 3299 Fragments arranged by size: 3299 1249 1071 990 825 708 198 85 BsmI GAATG_Cn' Cuts at: 5936 6029 5936 Size: 93 8332 BsmBI CGTCTCn'nnnn_ Cuts at: 3362 3503 4031 4512 5675 3362 Size: 141 528 481 1163 6112 Fragments arranged by size: 6112 1163 528 481 141 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2317 2420 2485 2516 2583 2751 2801 Size: 399 527 103 65 31 67 168 50 Cuts at: 2801 2822 2855 2940 3663 3672 3765 4229 4270 Size: 21 33 85 723 9 93 464 41 Cuts at: 4270 4291 4473 4529 4581 4652 4671 4775 4800 Size: 21 182 56 52 71 19 104 25 Cuts at: 4800 4857 5132 5223 5310 5352 5444 5496 5715 Size: 57 275 91 87 42 92 52 219 Cuts at: 5715 5744 6486 6571 7642 1391 Size: 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 723 527 464 399 275 219 182 168 104 103 93 92 91 87 85 85 71 67 65 57 56 52 52 50 42 41 33 31 29 25 21 21 19 9 BspEI T'CCGG_A Cuts at: 2835 3328 8088 2835 Size: 493 4760 3172 Fragments arranged by size: 4760 3172 493 BspHI T'CATG_A Cuts at: 2625 6314 8044 2625 Size: 3689 1730 3006 Fragments arranged by size: 3689 3006 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2460 2818 3202 3703 3870 4704 5698 5780 Size: 1616 358 384 501 167 834 994 82 Cuts at: 5780 844 Size: 3489 Fragments arranged by size: 3489 1616 994 834 501 384 358 167 82 BsrBI CCG'CTC Cuts at: 1394 1823 2643 6312 1394 Size: 429 820 3669 3507 Fragments arranged by size: 3669 3507 820 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2287 6920 7094 65 Size: 1183 1039 4633 174 1396 Fragments arranged by size: 4633 1396 1183 1039 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2358 4422 5464 5556 7069 7256 1700 Size: 110 548 2064 1042 92 1513 187 2869 Fragments arranged by size: 2869 2064 1513 1042 548 187 110 92 BsrGI T'GTAC_A Cuts at: 96 3114 96 Size: 3018 5407 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8425 BssSI C'ACGA_G Cuts at: 5345 6485 7497 5345 Size: 1140 1012 6273 Fragments arranged by size: 6273 1140 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3862 4629 4666 794 Size: 3068 767 37 4553 Fragments arranged by size: 4553 3068 767 37 BstBI TT'CG_AA Cuts at: 7229 7229 Size: 8425 BstEII G'GTnAC_C Cuts at: 1184 3533 1184 Size: 2349 6076 BstXI CCAn_nnnn'nTGG Cuts at: 3100 3679 3737 5263 3100 Size: 579 58 1526 6262 Fragments arranged by size: 6262 1526 579 58 BstYI r'GATC_y Cuts at: 1972 2991 3423 4002 5755 6122 6507 6524 7976 Size: 1019 432 579 1753 367 385 17 1452 Cuts at: 7976 8062 8074 8420 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1753 1452 1019 579 432 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3481 3799 3481 Size: 318 8107 BtgI C'CryG_G Cuts at: 513 1065 2004 3099 3538 3879 4551 513 Size: 552 939 1095 439 341 672 4387 Fragments arranged by size: 4387 1095 939 672 552 439 341 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5026 487 Size: 683 56 3800 3886 Fragments arranged by size: 3886 3800 683 56 BtsI GCAGTG_nn' Cuts at: 2998 4615 4932 5662 5941 6735 6755 2998 Size: 1617 317 730 279 794 20 4668 Fragments arranged by size: 4668 1617 794 730 317 279 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8390 DraI TTT'AAA Cuts at: 5729 5729 Size: 8425 DraIII CAC_nnn'GTG Cuts at: 2596 4629 4945 2596 Size: 2033 316 6076 Fragments arranged by size: 6076 2033 316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3593 5141 5540 7432 809 Size: 507 2277 1548 399 1892 1802 Fragments arranged by size: 2277 1892 1802 1548 507 399 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2676 3876 4011 Size: 54 1425 50 275 18 846 1200 135 Cuts at: 4011 4063 4143 4548 5526 5601 5872 6761 8390 Size: 52 80 405 978 75 271 889 1629 Cuts at: 8390 8 Size: 43 Fragments arranged by size: 1629 1425 1200 978 889 846 405 275 271 135 80 75 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4063 5872 1537 Size: 275 2251 1809 4090 Fragments arranged by size: 4090 2251 1809 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2333 2376 3080 3848 3993 4199 5006 Size: 774 206 43 704 768 145 206 807 Cuts at: 5006 6355 1353 Size: 1349 3423 Fragments arranged by size: 3423 1349 807 774 768 704 206 206 145 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2510 4392 4523 4985 7012 7386 Size: 1064 138 921 1882 131 462 2027 374 Cuts at: 7386 7532 387 Size: 146 1280 Fragments arranged by size: 2027 1882 1280 1064 921 462 374 146 138 131 EcoICRI GAG'CTC Cuts at: 2514 2514 Size: 8425 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2308 2500 2530 2681 2774 2888 2912 Size: 393 676 192 30 151 93 114 24 Cuts at: 2912 3278 3326 3386 3662 4517 4565 4787 4847 Size: 366 48 60 276 855 48 222 60 Cuts at: 4847 5039 6488 7085 7872 8140 1239 Size: 192 1449 597 787 268 1524 Fragments arranged by size: 1524 1449 855 787 676 597 393 366 276 268 222 192 192 151 114 93 60 60 48 48 30 24 EcoNI CCTnn'n_nnAGG Cuts at: 2037 2381 3293 3845 3866 4352 2037 Size: 344 912 552 21 486 6110 Fragments arranged by size: 6110 912 552 486 344 21 EcoO109I rG'GnC_Cy Cuts at: 2069 2313 2481 2482 2500 2798 2812 3070 3668 Size: 244 168 1 18 298 14 258 598 Cuts at: 3668 3859 3871 4287 4288 4719 4762 4796 4854 Size: 191 12 416 1 431 43 34 58 Cuts at: 4854 5306 5307 5668 2069 Size: 452 1 361 4826 Fragments arranged by size: 4826 598 452 431 416 361 298 258 244 191 168 58 43 34 18 14 12 1 1 1 EcoRI G'AATT_C Cuts at: 5734 6112 5734 Size: 378 8047 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3202 3202 Size: 8425 FspI TGC'GCA Cuts at: 2427 2469 2687 5552 6931 2427 Size: 42 218 2865 1379 3921 Fragments arranged by size: 3921 2865 1379 218 42 FspAI rTGC'GCAy Cuts at: 2427 2469 2427 Size: 42 8383 HaeII r_GCGC'y Cuts at: 1678 2948 2955 3701 3783 5471 5646 7572 1678 Size: 1270 7 746 82 1688 175 1926 2531 Fragments arranged by size: 2531 1926 1688 1270 746 175 82 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2332 2364 3323 3355 3934 Size: 32 135 32 760 32 959 32 579 Cuts at: 3934 3966 4639 4671 7066 7098 7140 7172 1373 Size: 32 673 32 2395 32 42 32 2626 Fragments arranged by size: 2626 2395 959 760 673 579 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3477 3516 4129 4309 5769 6015 Size: 988 281 1539 39 613 180 1460 246 Cuts at: 6015 669 Size: 3079 Fragments arranged by size: 3079 1539 1460 988 613 281 246 180 39 HindIII A'AGCT_T Cuts at: 748 4900 748 Size: 4152 4273 HpaI GTT'AAC Cuts at: 6015 6015 Size: 8425 KasI G'GCGC_C Cuts at: 3779 5467 3779 Size: 1688 6737 KpnI G_GTAC'C Cuts at: 5750 5750 Size: 8425 MfeI C'AATT_G Cuts at: 6024 6024 Size: 8425 MluI A'CGCG_T Cuts at: 8301 8301 Size: 8425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2040 4586 7539 7723 1305 Size: 735 2546 2953 184 2007 Fragments arranged by size: 2953 2546 2007 735 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4145 5603 10 Size: 54 1425 343 2313 1458 2832 Fragments arranged by size: 2832 2313 1458 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 3944 4222 5085 6383 6742 6901 Size: 648 1095 1683 278 863 1298 359 159 Cuts at: 6901 518 Size: 2042 Fragments arranged by size: 2042 1683 1298 1095 863 648 359 278 159 MspA1I CmG'CkG Cuts at: 1606 2050 2243 2294 2328 3349 3433 3490 3496 Size: 444 193 51 34 1021 84 57 6 Cuts at: 3496 3540 3640 4746 4848 4959 5446 5841 6518 Size: 44 100 1106 102 111 487 395 677 Cuts at: 6518 7666 7911 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1106 1021 677 487 444 395 245 193 111 102 100 84 57 51 44 34 6 NaeI GCC'GGC Cuts at: 5466 5558 5466 Size: 92 8333 NarI GG'CG_CC Cuts at: 3780 5468 3780 Size: 1688 6737 NcoI C'CATG_G Cuts at: 513 1065 2004 3099 513 Size: 552 939 1095 5839 Fragments arranged by size: 5839 1095 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8425 NgoMIV G'CCGG_C Cuts at: 5464 5556 5464 Size: 92 8333 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8425 NotI GC'GGCC_GC Cuts at: 5872 5872 Size: 8425 NspI r_CATG'y Cuts at: 1132 1229 3304 3592 5783 7328 1132 Size: 97 2075 288 2191 1545 2229 Fragments arranged by size: 2229 2191 2075 1545 288 97 PasI CC'CwG_GG Cuts at: 1348 2152 3318 3664 1348 Size: 804 1166 346 6109 Fragments arranged by size: 6109 1166 804 346 PciI A'CATG_T Cuts at: 7324 7324 Size: 8425 PflMI CCAn_nnn'nTGG Cuts at: 1108 4633 1108 Size: 3525 4900 PfoI T'CCnGG_A Cuts at: 2056 2751 2904 3846 4158 4488 4581 4869 2056 Size: 695 153 942 312 330 93 288 5612 Fragments arranged by size: 5612 942 695 330 312 288 153 93 PmeI GTTT'AAAC Cuts at: 5729 5729 Size: 8425 PmlI CAC'GTG Cuts at: 5368 5368 Size: 8425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4412 4444 6494 6526 8007 8039 4412 Size: 32 2050 32 1481 32 4798 Fragments arranged by size: 4798 2050 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2500 3070 3859 3871 4719 4762 2500 Size: 570 789 12 848 43 6163 Fragments arranged by size: 6163 848 789 570 43 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8425 PsiI TTA'TAA Cuts at: 5995 5995 Size: 8425 PspOMI G'GGCC_C Cuts at: 2313 2481 2797 3668 4287 4796 4853 5306 5440 Size: 168 316 871 619 509 57 453 134 Cuts at: 5440 2313 Size: 5298 Fragments arranged by size: 5298 871 619 509 453 316 168 134 57 PspXI vC'TCGA_Gb Cuts at: 3191 3191 Size: 8425 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2441 2473 2441 Size: 32 8393 PstI C_TGCA'G Cuts at: 830 3348 3858 3867 3975 4281 4646 4662 4713 Size: 2518 510 9 108 306 365 16 51 Cuts at: 4713 4890 5777 830 Size: 177 887 3478 Fragments arranged by size: 3478 2518 887 510 365 306 177 108 51 16 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8425 PvuII CAG'CTG Cuts at: 2050 2243 2328 3349 3433 3496 3640 4848 2050 Size: 193 85 1021 84 63 144 1208 5627 Fragments arranged by size: 5627 1208 1021 193 144 85 84 63 SacI G_AGCT'C Cuts at: 2516 2516 Size: 8425 SacII CC_GC'GG Cuts at: 3541 3541 Size: 8425 SalI G'TCGA_C Cuts at: 1936 5767 1936 Size: 3831 4594 SanDI GG'GwC_CC Cuts at: 4719 4719 Size: 8425 SapI GCTCTTCn'nnn_ Cuts at: 4199 4199 Size: 8425 SbfI CC_TGCA'GG Cuts at: 4713 5777 4713 Size: 1064 7361 ScaI AGT'ACT Cuts at: 6673 8385 6673 Size: 1712 6713 SexAI A'CCwGG_T Cuts at: 3411 3411 Size: 8425 SfcI C'TryA_G Cuts at: 826 1045 2533 3344 3854 3863 3971 4277 4603 Size: 219 1488 811 510 9 108 306 326 Cuts at: 4603 4642 4658 4709 4886 5773 6908 7589 7780 Size: 39 16 51 177 887 1135 681 191 Cuts at: 7780 826 Size: 1471 Fragments arranged by size: 1488 1471 1135 887 811 681 510 326 306 219 191 177 108 51 39 16 9 SfoI GGC'GCC Cuts at: 3781 5469 3781 Size: 1688 6737 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8425 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8425 SmaI CCC'GGG Cuts at: 1872 2437 4265 4801 4859 5312 5439 5752 1872 Size: 565 1828 536 58 453 127 313 4545 Fragments arranged by size: 4545 1828 565 536 453 313 127 58 SmlI C'TyrA_G Cuts at: 820 1017 1943 3191 3200 3951 6529 7430 7692 Size: 197 926 1248 9 751 2578 901 262 Cuts at: 7692 820 Size: 1553 Fragments arranged by size: 2578 1553 1248 926 901 751 262 197 9 SnaBI TAC'GTA Cuts at: 493 6216 493 Size: 5723 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8425 SphI G_CATG'C Cuts at: 1132 5783 1132 Size: 4651 3774 SrfI GCCC'GGGC Cuts at: 4801 4801 Size: 8425 SspI AAT'ATT Cuts at: 5 52 6349 5 Size: 47 6297 2081 Fragments arranged by size: 6297 2081 47 StuI AGG'CCT Cuts at: 1517 3167 3791 1517 Size: 1650 624 6151 Fragments arranged by size: 6151 1650 624 StyI C'CwwG_G Cuts at: 513 1065 2004 2503 2649 2940 3099 3558 5864 Size: 552 939 499 146 291 159 459 2306 Cuts at: 5864 513 Size: 3074 Fragments arranged by size: 3074 2306 939 552 499 459 291 159 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2263 4640 5113 5536 6452 6469 6622 Size: 1255 683 2377 473 423 916 17 153 Cuts at: 6622 6807 7252 325 Size: 185 445 1498 Fragments arranged by size: 2377 1498 1255 916 683 473 445 423 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3114 6671 8383 Size: 274 80 33 51 1443 1137 3557 1712 Cuts at: 8383 96 Size: 138 Fragments arranged by size: 3557 1712 1443 1137 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2195 2227 2503 2535 2582 2614 2990 3022 3828 Size: 32 276 32 47 32 376 32 806 Cuts at: 3828 3860 4333 4365 4543 4575 4823 4855 8385 Size: 32 473 32 178 32 248 32 3530 Cuts at: 8385 8417 2195 Size: 32 2203 Fragments arranged by size: 3530 2203 806 473 376 276 248 178 47 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2402 3230 1316 Size: 1086 828 6511 Fragments arranged by size: 6511 1086 828 XbaI T'CTAG_A Cuts at: 5761 5761 Size: 8425 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2656 4078 4700 5521 2149 Size: 507 1422 622 821 5053 Fragments arranged by size: 5053 1422 821 622 507 XhoI C'TCGA_G Cuts at: 1943 3191 1943 Size: 1248 7177 XmaI C'CCGG_G Cuts at: 1870 2435 4263 4799 4857 5310 5437 5750 1870 Size: 565 1828 536 58 453 127 313 4545 Fragments arranged by size: 4545 1828 565 536 453 313 127 58 XmnI GAAnn'nnTTC Cuts at: 3134 6554 3134 Size: 3420 5005 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2404 2716 5542 6233 Size: 53 83 186 936 870 312 2826 691 Cuts at: 6233 276 Size: 2468 Fragments arranged by size: 2826 2468 936 870 691 312 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI NruI NsiI PacI RsrII SfiI SwaI