(Linear) (Six-base) MAPSORT of: KIEE3530.seq Check: 5983 from: 1 to: 3720 KIEE3530.orf With 182 enzymes: SgfI * July 3, 2012 18:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 813 1197 1698 2699 3693 3720 Size: 813 384 501 1001 994 27 Fragments arranged by size: 1001 994 813 501 384 27 AatII G_ACGT'C Cuts at: 0 401 713 3539 3720 Size: 401 312 2826 181 Fragments arranged by size: 2826 401 312 181 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 907 1273 1321 3720 Size: 907 366 48 2399 Fragments arranged by size: 2399 907 366 48 AfeI AGC'GCT Cuts at: 0 3639 3720 Size: 3639 81 AgeI A'CCGG_T Cuts at: 0 353 3720 Size: 353 3367 AhdI GACnn_n'nnGTC Cuts at: 0 1332 3720 Size: 1332 2388 AleI CACnn'nnGTG Cuts at: 0 256 3080 3720 Size: 256 2824 640 Fragments arranged by size: 2824 640 256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 274 1669 3415 3720 Size: 274 1395 1746 305 Fragments arranged by size: 1746 1395 305 274 AlwNI CAG_nnn'CTG Cuts at: 0 238 1428 1848 1857 1929 1974 2461 2533 Size: 238 1190 420 9 72 45 487 72 Cuts at: 2533 2628 3111 3720 Size: 95 483 609 Fragments arranged by size: 1190 609 487 483 420 238 95 72 72 45 9 ApaI G_GGCC'C Cuts at: 0 312 480 796 1667 2286 2795 2852 3305 Size: 312 168 316 871 619 509 57 453 Cuts at: 3305 3439 3720 Size: 134 281 Fragments arranged by size: 871 619 509 453 316 312 281 168 134 57 AvaI C'yCGr_G Cuts at: 0 430 540 1186 2258 2569 2794 2852 3305 Size: 430 110 646 1072 311 225 58 453 Cuts at: 3305 3432 3720 Size: 127 288 Fragments arranged by size: 1072 646 453 430 311 288 225 127 110 58 AvrII C'CTAG_G Cuts at: 0 498 3720 Size: 498 3222 BanI G'GyrC_C Cuts at: 0 258 1653 1735 1774 1915 1955 2219 2617 Size: 258 1395 82 39 141 40 264 398 Cuts at: 2617 2980 3462 3705 3720 Size: 363 482 243 15 Fragments arranged by size: 1395 482 398 363 264 258 243 141 82 40 39 15 BanII G_rGCy'C Cuts at: 0 312 415 480 511 578 746 796 817 Size: 312 103 65 31 67 168 50 21 Cuts at: 817 850 935 1667 2286 2524 2576 2647 2770 Size: 33 85 732 619 238 52 71 123 Cuts at: 2770 2795 2852 3305 3347 3439 3720 Size: 25 57 453 42 92 281 Fragments arranged by size: 732 619 453 312 281 238 168 123 103 92 85 71 67 65 57 52 50 42 33 31 25 21 BbeI G_GCGC'C Cuts at: 0 1778 3466 3720 Size: 1778 1688 254 Fragments arranged by size: 1778 1688 254 BbsI GAAGACnn'nnnn_ Cuts at: 0 667 1560 3720 Size: 667 893 2160 Fragments arranged by size: 2160 893 667 BbvCI CC'TCA_GC Cuts at: 0 818 2888 3296 3311 3720 Size: 818 2070 408 15 409 Fragments arranged by size: 2070 818 409 408 15 BciVI GTATCCnnnnn_n' Cuts at: 0 887 3720 Size: 887 2833 BglI GCCn_nnn'nGGC Cuts at: 0 1662 1783 2175 2836 3238 3330 3365 3720 Size: 1662 121 392 661 402 92 35 355 Fragments arranged by size: 1662 661 402 392 355 121 92 35 BlpI GC'TnA_GC Cuts at: 0 943 3720 Size: 943 2777 Bme1580I G_kGCm'C Cuts at: 0 312 480 796 1658 1667 2224 2265 2286 Size: 312 168 316 862 9 557 41 21 Cuts at: 2286 2795 2852 3127 3218 3305 3439 3710 3720 Size: 509 57 275 91 87 134 271 10 Fragments arranged by size: 862 557 509 316 312 275 271 168 134 91 87 57 41 21 10 9 BmrI ACTGGGnnnn_n' Cuts at: 0 2616 3720 Size: 2616 1104 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2019 3522 3606 3720 Size: 2019 1503 84 114 Fragments arranged by size: 2019 1503 114 84 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 303 495 525 676 769 883 1381 1657 Size: 303 192 30 151 93 114 498 276 Cuts at: 1657 2512 2560 2782 2842 3034 3720 Size: 855 48 222 60 192 686 Fragments arranged by size: 855 686 498 303 276 222 192 192 151 114 93 60 48 30 Bpu10I CC'TnA_GC Cuts at: 0 811 818 2104 2888 3296 3311 3720 Size: 811 7 1286 784 408 15 409 Fragments arranged by size: 1286 811 784 409 408 15 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1180 1931 3720 Size: 1180 751 1789 Fragments arranged by size: 1789 1180 751 BsaI GGTCTCn'nnnn_ Cuts at: 0 1510 3001 3207 3720 Size: 1510 1491 206 513 Fragments arranged by size: 1510 1491 513 206 BsaAI yAC'GTr Cuts at: 0 876 1122 3363 3720 Size: 876 246 2241 357 Fragments arranged by size: 2241 876 357 246 BsaBI GATnn'nnATC Cuts at: 0 422 3034 3720 Size: 422 2612 686 Fragments arranged by size: 2612 686 422 BsaHI Gr'CG_yC Cuts at: 0 152 398 710 1775 2957 3463 3536 3569 Size: 152 246 312 1065 1182 506 73 33 Cuts at: 3569 3720 Size: 151 Fragments arranged by size: 1182 1065 506 312 246 152 151 73 33 BsaWI w'CCGG_w Cuts at: 0 353 830 1323 3720 Size: 353 477 493 2397 Fragments arranged by size: 2397 493 477 353 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 190 220 498 528 985 1015 1507 1537 Size: 190 30 278 30 457 30 492 30 Cuts at: 1537 2634 2664 3720 Size: 1097 30 1056 Fragments arranged by size: 1097 1056 492 457 278 190 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 8 60 562 742 745 2173 2244 2509 Size: 8 52 502 180 3 1428 71 265 Cuts at: 2509 2512 2584 2964 3304 3328 3511 3682 3720 Size: 3 72 380 340 24 183 171 38 Fragments arranged by size: 1428 502 380 340 265 183 180 171 72 71 52 38 24 8 3 3 BseYI C'CCAG_C Cuts at: 0 157 231 472 614 777 789 2316 3720 Size: 157 74 241 142 163 12 1527 1404 Fragments arranged by size: 1527 1404 241 163 157 142 74 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 682 877 976 1233 1726 1894 3720 Size: 682 195 99 257 493 168 1826 Fragments arranged by size: 1826 682 493 257 195 168 99 BsiEI CG_ry'CG Cuts at: 0 2061 3720 Size: 2061 1659 BsiHKAI G_wGCw'C Cuts at: 0 511 1760 2468 2666 3491 3720 Size: 511 1249 708 198 825 229 Fragments arranged by size: 1249 825 708 511 229 198 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1357 1498 2026 2507 3670 3720 Size: 1357 141 528 481 1163 50 Fragments arranged by size: 1357 1163 528 481 141 50 Bsp1286I G_dGCh'C Cuts at: 0 312 415 480 511 578 746 796 817 Size: 312 103 65 31 67 168 50 21 Cuts at: 817 850 935 1658 1667 1760 2224 2265 2286 Size: 33 85 723 9 93 464 41 21 Cuts at: 2286 2468 2524 2576 2647 2666 2770 2795 2852 Size: 182 56 52 71 19 104 25 57 Cuts at: 2852 3127 3218 3305 3347 3439 3491 3710 3720 Size: 275 91 87 42 92 52 219 10 Fragments arranged by size: 723 464 312 275 219 182 168 104 103 93 92 91 87 85 71 67 65 57 56 52 52 50 42 41 33 31 25 21 21 19 10 9 BspEI T'CCGG_A Cuts at: 0 830 1323 3720 Size: 830 493 2397 Fragments arranged by size: 2397 830 493 BspHI T'CATG_A Cuts at: 0 620 3720 Size: 620 3100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 455 813 1197 1698 1865 2699 3693 3720 Size: 455 358 384 501 167 834 994 27 Fragments arranged by size: 994 834 501 455 384 358 167 27 BsrBI CCG'CTC Cuts at: 0 638 3720 Size: 638 3082 BsrDI GCAATG_nn' Cuts at: 0 282 3720 Size: 282 3438 BsrFI r'CCGG_y Cuts at: 0 353 2417 3459 3551 3720 Size: 353 2064 1042 92 169 Fragments arranged by size: 2064 1042 353 169 92 BsrGI T'GTAC_A Cuts at: 0 1109 3720 Size: 1109 2611 BssSI C'ACGA_G Cuts at: 0 3340 3720 Size: 3340 380 BstAPI GCAn_nnn'nTGC Cuts at: 0 1857 2624 2661 3720 Size: 1857 767 37 1059 Fragments arranged by size: 1857 1059 767 37 BstEII G'GTnAC_C Cuts at: 0 1528 3720 Size: 1528 2192 BstXI CCAn_nnnn'nTGG Cuts at: 0 1095 1674 1732 3258 3720 Size: 1095 579 58 1526 462 Fragments arranged by size: 1526 1095 579 462 58 BstYI r'GATC_y Cuts at: 0 986 1418 1997 3720 Size: 986 432 579 1723 Fragments arranged by size: 1723 986 579 432 Bsu36I CC'TnA_GG Cuts at: 0 1476 1794 3720 Size: 1476 318 1926 Fragments arranged by size: 1926 1476 318 BtgI C'CryG_G Cuts at: 0 1094 1533 1874 2546 3720 Size: 1094 439 341 672 1174 Fragments arranged by size: 1174 1094 672 439 341 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3021 3720 Size: 3021 699 BtsI GCAGTG_nn' Cuts at: 0 993 2610 2927 3657 3720 Size: 993 1617 317 730 63 Fragments arranged by size: 1617 993 730 317 63 DraIII CAC_nnn'GTG Cuts at: 0 591 2624 2940 3720 Size: 591 2033 316 780 Fragments arranged by size: 2033 780 591 316 DrdI GACnn_nn'nnGTC Cuts at: 0 1588 3136 3535 3720 Size: 1588 1548 399 185 Fragments arranged by size: 1588 1548 399 185 EaeI y'GGCC_r Cuts at: 0 671 1871 2006 2058 2138 2543 3521 3596 Size: 671 1200 135 52 80 405 978 75 Cuts at: 3596 3720 Size: 124 Fragments arranged by size: 1200 978 671 405 135 124 80 75 52 EagI C'GGCC_G Cuts at: 0 2058 3720 Size: 2058 1662 EarI CTCTTCn'nnn_ Cuts at: 0 122 328 371 1075 1843 1988 2194 3001 Size: 122 206 43 704 768 145 206 807 Cuts at: 3001 3720 Size: 719 Fragments arranged by size: 807 768 719 704 206 206 145 122 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 505 2387 2518 2980 3720 Size: 505 1882 131 462 740 Fragments arranged by size: 1882 740 505 462 131 EcoICRI GAG'CTC Cuts at: 0 509 3720 Size: 509 3211 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 303 495 525 676 769 883 907 1273 Size: 303 192 30 151 93 114 24 366 Cuts at: 1273 1321 1381 1657 2512 2560 2782 2842 3034 Size: 48 60 276 855 48 222 60 192 Cuts at: 3034 3720 Size: 686 Fragments arranged by size: 855 686 366 303 276 222 192 192 151 114 93 60 60 48 48 30 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 32 376 1288 1840 1861 2347 3720 Size: 32 344 912 552 21 486 1373 Fragments arranged by size: 1373 912 552 486 344 32 21 EcoO109I rG'GnC_Cy Cuts at: 0 64 308 476 477 495 793 807 1065 Size: 64 244 168 1 18 298 14 258 Cuts at: 1065 1663 1854 1866 2282 2283 2714 2757 2791 Size: 598 191 12 416 1 431 43 34 Cuts at: 2791 2849 3301 3302 3663 3720 Size: 58 452 1 361 57 Fragments arranged by size: 598 452 431 416 361 298 258 244 191 168 64 58 57 43 34 18 14 12 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1197 3720 Size: 1197 2523 FspI TGC'GCA Cuts at: 0 422 464 682 3547 3720 Size: 422 42 218 2865 173 Fragments arranged by size: 2865 422 218 173 42 FspAI rTGC'GCAy Cuts at: 0 422 464 3720 Size: 422 42 3256 Fragments arranged by size: 3256 422 42 HaeII r_GCGC'y Cuts at: 0 943 950 1696 1778 3466 3641 3720 Size: 943 7 746 82 1688 175 79 Fragments arranged by size: 1688 943 746 175 82 79 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 327 359 1318 1350 1929 1961 2634 2666 Size: 327 32 959 32 579 32 673 32 Cuts at: 2666 3720 Size: 1054 Fragments arranged by size: 1054 959 673 579 327 32 32 32 32 HincII GTy'rAC Cuts at: 0 1472 1511 2124 2304 3720 Size: 1472 39 613 180 1416 Fragments arranged by size: 1472 1416 613 180 39 HindIII A'AGCT_T Cuts at: 0 2895 3720 Size: 2895 825 KasI G'GCGC_C Cuts at: 0 1774 3462 3720 Size: 1774 1688 258 Fragments arranged by size: 1774 1688 258 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 35 2581 3720 Size: 35 2546 1139 Fragments arranged by size: 2546 1139 35 MscI TGG'CCA Cuts at: 0 2140 3598 3720 Size: 2140 1458 122 Fragments arranged by size: 2140 1458 122 MslI CAynn'nnrTG Cuts at: 0 256 1939 2217 3080 3720 Size: 256 1683 278 863 640 Fragments arranged by size: 1683 863 640 278 256 MspA1I CmG'CkG Cuts at: 0 45 238 289 323 1344 1428 1485 1491 Size: 45 193 51 34 1021 84 57 6 Cuts at: 1491 1535 1635 2741 2843 2954 3441 3720 Size: 44 100 1106 102 111 487 279 Fragments arranged by size: 1106 1021 487 279 193 111 102 100 84 57 51 45 44 34 6 NaeI GCC'GGC Cuts at: 0 3461 3553 3720 Size: 3461 92 167 Fragments arranged by size: 3461 167 92 NarI GG'CG_CC Cuts at: 0 1775 3463 3720 Size: 1775 1688 257 Fragments arranged by size: 1775 1688 257 NcoI C'CATG_G Cuts at: 0 1094 3720 Size: 1094 2626 NgoMIV G'CCGG_C Cuts at: 0 3459 3551 3720 Size: 3459 92 169 Fragments arranged by size: 3459 169 92 NspI r_CATG'y Cuts at: 0 1299 1587 3720 Size: 1299 288 2133 Fragments arranged by size: 2133 1299 288 PasI CC'CwG_GG Cuts at: 0 147 1313 1659 3720 Size: 147 1166 346 2061 Fragments arranged by size: 2061 1166 346 147 PflMI CCAn_nnn'nTGG Cuts at: 0 2628 3720 Size: 2628 1092 PfoI T'CCnGG_A Cuts at: 0 51 746 899 1841 2153 2483 2576 2864 Size: 51 695 153 942 312 330 93 288 Cuts at: 2864 3720 Size: 856 Fragments arranged by size: 942 856 695 330 312 288 153 93 51 PmlI CAC'GTG Cuts at: 0 3363 3720 Size: 3363 357 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2407 2439 3720 Size: 2407 32 1281 Fragments arranged by size: 2407 1281 32 PpuMI rG'GwC_Cy Cuts at: 0 495 1065 1854 1866 2714 2757 3720 Size: 495 570 789 12 848 43 963 Fragments arranged by size: 963 848 789 570 495 43 12 PspOMI G'GGCC_C Cuts at: 0 308 476 792 1663 2282 2791 2848 3301 Size: 308 168 316 871 619 509 57 453 Cuts at: 3301 3435 3720 Size: 134 285 Fragments arranged by size: 871 619 509 453 316 308 285 168 134 57 PspXI vC'TCGA_Gb Cuts at: 0 1186 3720 Size: 1186 2534 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 436 468 3720 Size: 436 32 3252 Fragments arranged by size: 3252 436 32 PstI C_TGCA'G Cuts at: 0 1343 1853 1862 1970 2276 2641 2657 2708 Size: 1343 510 9 108 306 365 16 51 Cuts at: 2708 2885 3720 Size: 177 835 Fragments arranged by size: 1343 835 510 365 306 177 108 51 16 9 PvuII CAG'CTG Cuts at: 0 45 238 323 1344 1428 1491 1635 2843 Size: 45 193 85 1021 84 63 144 1208 Cuts at: 2843 3720 Size: 877 Fragments arranged by size: 1208 1021 877 193 144 85 84 63 45 SacI G_AGCT'C Cuts at: 0 511 3720 Size: 511 3209 SacII CC_GC'GG Cuts at: 0 1536 3720 Size: 1536 2184 SanDI GG'GwC_CC Cuts at: 0 2714 3720 Size: 2714 1006 SapI GCTCTTCn'nnn_ Cuts at: 0 2194 3720 Size: 2194 1526 SbfI CC_TGCA'GG Cuts at: 0 2708 3720 Size: 2708 1012 SexAI A'CCwGG_T Cuts at: 0 1406 3720 Size: 1406 2314 SfcI C'TryA_G Cuts at: 0 528 1339 1849 1858 1966 2272 2598 2637 Size: 528 811 510 9 108 306 326 39 Cuts at: 2637 2653 2704 2881 3720 Size: 16 51 177 839 Fragments arranged by size: 839 811 528 510 326 306 177 108 51 39 16 9 SfoI GGC'GCC Cuts at: 0 1776 3464 3720 Size: 1776 1688 256 Fragments arranged by size: 1776 1688 256 SmaI CCC'GGG Cuts at: 0 432 2260 2796 2854 3307 3434 3720 Size: 432 1828 536 58 453 127 286 Fragments arranged by size: 1828 536 453 432 286 127 58 SmlI C'TyrA_G Cuts at: 0 1186 1195 1946 3720 Size: 1186 9 751 1774 Fragments arranged by size: 1774 1186 751 9 SrfI GCCC'GGGC Cuts at: 0 2796 3720 Size: 2796 924 StuI AGG'CCT Cuts at: 0 1162 1786 3720 Size: 1162 624 1934 Fragments arranged by size: 1934 1162 624 StyI C'CwwG_G Cuts at: 0 498 644 935 1094 1553 3720 Size: 498 146 291 159 459 2167 Fragments arranged by size: 2167 498 459 291 159 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 258 2635 3108 3531 3720 Size: 258 2377 473 423 189 Fragments arranged by size: 2377 473 423 258 189 TatI w'GTAC_w Cuts at: 0 1109 3720 Size: 1109 2611 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 190 222 498 530 577 609 985 1017 Size: 190 32 276 32 47 32 376 32 Cuts at: 1017 1823 1855 2328 2360 2538 2570 2818 2850 Size: 806 32 473 32 178 32 248 32 Cuts at: 2850 3720 Size: 870 Fragments arranged by size: 870 806 473 376 276 248 190 178 47 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 397 1225 3720 Size: 397 828 2495 Fragments arranged by size: 2495 828 397 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 144 651 2073 2695 3516 3720 Size: 144 507 1422 622 821 204 Fragments arranged by size: 1422 821 622 507 204 144 XhoI C'TCGA_G Cuts at: 0 1186 3720 Size: 1186 2534 XmaI C'CCGG_G Cuts at: 0 430 2258 2794 2852 3305 3432 3720 Size: 430 1828 536 58 453 127 288 Fragments arranged by size: 1828 536 453 430 288 127 58 XmnI GAAnn'nnTTC Cuts at: 0 1129 3720 Size: 1129 2591 ZraI GAC'GTC Cuts at: 0 399 711 3537 3720 Size: 399 312 2826 183 Fragments arranged by size: 2826 399 312 183 Enzymes that do cut: AarI AatII AcuI AfeI AgeI AhdI AleI AlfI AlwNI ApaI AvaI AvrII BanI BanII BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PsrI PstI PvuII SacI SacII SanDI SapI SbfI SexAI SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AflIII AloI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BcgI BclI BfrBI BglII BmgBI BmtI BsiWI BsmI BssHII BstBI BstZ17I ClaI CspCI DraI EcoRI EcoRV FseI HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PvuI RsrII SalI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI XbaI