(Circular) (Six-base) MAPSORT of: pFN21AB6875.seq Check: 8132 from: 1 to: 5182 pFN21AB6875 5182 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2992 278 Size: 53 83 186 936 1456 2468 Fragments arranged by size: 2468 1456 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2525 1937 Size: 588 4594 Acc65I G'GTAC_C Cuts at: 2170 2503 2170 Size: 333 4849 AclI AA'CG_TT Cuts at: 1563 3309 3682 1563 Size: 1746 373 3063 Fragments arranged by size: 3063 1746 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2047 3245 4629 4897 1632 Size: 415 1198 1384 268 1917 Fragments arranged by size: 1917 1384 1198 415 268 AfeI AGC'GCT Cuts at: 2315 2315 Size: 5182 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4985 AflIII A'CryG_T Cuts at: 1203 4081 5058 1203 Size: 2878 977 1327 Fragments arranged by size: 2878 1327 977 AgeI A'CCGG_T Cuts at: 4013 4013 Size: 5182 AhdI GACnn_n'nnGTC Cuts at: 2141 3911 2141 Size: 1770 3412 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5182 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5182 AlwNI CAG_nnn'CTG Cuts at: 2070 2925 4497 2070 Size: 855 1572 2755 Fragments arranged by size: 2755 1572 855 ApaLI G'TGCA_C Cuts at: 3239 4395 3239 Size: 1156 4026 ApoI r'AATT_y Cuts at: 1493 2459 2491 2717 2869 4978 1493 Size: 966 32 226 152 2109 1697 Fragments arranged by size: 2109 1697 966 226 152 32 AseI AT'TA_AT Cuts at: 160 1032 3736 160 Size: 872 2704 1606 Fragments arranged by size: 2704 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5182 AvaI C'yCGr_G Cuts at: 1870 1943 2507 1870 Size: 73 564 4545 Fragments arranged by size: 4545 564 73 BamHI G'GATC_C Cuts at: 2512 2879 2512 Size: 367 4815 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2170 2503 3958 618 Size: 325 217 627 383 333 1455 1842 Fragments arranged by size: 1842 1455 627 383 333 325 217 BanII G_rGCy'C Cuts at: 1391 2501 1391 Size: 1110 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2093 928 Size: 971 194 4017 Fragments arranged by size: 4017 971 194 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3373 3407 1903 Size: 34 1436 34 3678 Fragments arranged by size: 3678 1436 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2117 3074 4284 2117 Size: 957 1210 3015 Fragments arranged by size: 3015 1210 957 BclI T'GATC_A Cuts at: 1548 1548 Size: 5182 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3793 136 Size: 107 122 71 631 605 2121 1525 Fragments arranged by size: 2121 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5177 5177 Size: 5182 BlpI GC'TnA_GC Cuts at: 2599 2599 Size: 5182 Bme1580I G_kGCm'C Cuts at: 1790 3243 4399 1790 Size: 1453 1156 2573 Fragments arranged by size: 2573 1453 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5182 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2913 3871 442 Size: 416 53 83 399 24 1496 958 1753 Fragments arranged by size: 1753 1496 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5182 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 2285 3842 1239 Size: 1004 42 1557 2579 Fragments arranged by size: 2579 1557 1004 42 Bpu10I CC'TnA_GC Cuts at: 2288 2288 Size: 5182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3307 4172 4470 3307 Size: 865 298 4019 Fragments arranged by size: 4019 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2243 3845 882 Size: 617 744 1602 2219 Fragments arranged by size: 2219 1602 744 617 BsaAI yAC'GTr Cuts at: 493 1204 2973 493 Size: 711 1769 2702 Fragments arranged by size: 2702 1769 711 BsaBI GATnn'nnATC Cuts at: 5176 5176 Size: 5182 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2445 2989 3371 4006 Size: 53 83 186 936 912 544 382 635 Cuts at: 4006 275 Size: 1451 Fragments arranged by size: 1451 936 912 635 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3615 4013 4287 4434 4845 3615 Size: 398 274 147 411 3952 Fragments arranged by size: 3952 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4957 Fragments arranged by size: 4957 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5182 BseYI C'CCAG_C Cuts at: 4385 4881 4385 Size: 496 4686 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2374 1163 Size: 573 638 3971 Fragments arranged by size: 3971 638 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2632 3393 4421 1540 Size: 275 186 631 761 1028 2301 Fragments arranged by size: 2301 1028 761 631 275 186 BsiHKAI G_wGCw'C Cuts at: 3243 3328 4399 3243 Size: 85 1071 4026 Fragments arranged by size: 4026 1071 85 BsmI GAATG_Cn' Cuts at: 2693 2786 2693 Size: 93 5089 BsmBI CGTCTCn'nnnn_ Cuts at: 2009 2009 Size: 5182 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2501 3243 3328 4399 1391 Size: 399 711 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 711 399 85 BspEI T'CCGG_A Cuts at: 4845 4845 Size: 5182 BspHI T'CATG_A Cuts at: 3071 4801 3071 Size: 1730 3452 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2537 844 Size: 1693 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2331 3069 1394 Size: 429 508 738 3507 Fragments arranged by size: 3507 738 508 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3677 3851 65 Size: 1183 2429 174 1396 Fragments arranged by size: 2429 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3826 4013 1700 Size: 110 2016 187 2869 Fragments arranged by size: 2869 2016 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5182 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5182 BssSI C'ACGA_G Cuts at: 3242 4254 3242 Size: 1012 4170 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5182 BstBI TT'CG_AA Cuts at: 3986 3986 Size: 5182 BstEII G'GTnAC_C Cuts at: 1184 2452 1184 Size: 1268 3914 BstYI r'GATC_y Cuts at: 1972 2512 2879 3264 3281 4733 4819 4831 5177 Size: 540 367 385 17 1452 86 12 346 Cuts at: 5177 1972 Size: 1977 Fragments arranged by size: 1977 1452 540 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2206 513 Size: 552 939 202 3489 Fragments arranged by size: 3489 939 552 202 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2411 487 Size: 683 56 1185 3258 Fragments arranged by size: 3258 1185 683 56 BtsI GCAGTG_nn' Cuts at: 2698 3492 3512 2698 Size: 794 20 4368 Fragments arranged by size: 4368 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5182 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2338 2373 529 Size: 35 1774 35 3338 Fragments arranged by size: 3338 1774 35 35 DraI TTT'AAA Cuts at: 2486 2486 Size: 5182 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2414 2444 4189 809 Size: 507 1098 30 1745 1802 Fragments arranged by size: 1802 1745 1098 507 30 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2629 3518 5147 Size: 54 1425 50 275 18 799 889 1629 Cuts at: 5147 8 Size: 43 Fragments arranged by size: 1629 1425 889 799 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2629 1537 Size: 275 817 4090 Fragments arranged by size: 4090 817 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3112 1353 Size: 1759 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3769 4143 4289 387 Size: 1064 138 2180 374 146 1280 Fragments arranged by size: 2180 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2243 2285 3245 3842 4629 4897 Size: 393 415 196 42 960 597 787 268 Cuts at: 4897 1239 Size: 1524 Fragments arranged by size: 1524 960 787 597 415 393 268 196 42 EcoNI CCTnn'n_nnAGG Cuts at: 2439 2439 Size: 5182 EcoRI G'AATT_C Cuts at: 2491 2869 2491 Size: 378 4804 FspI TGC'GCA Cuts at: 3688 3688 Size: 5182 HaeII r_GCGC'y Cuts at: 1678 2317 4329 1678 Size: 639 2012 2531 Fragments arranged by size: 2531 2012 639 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 2127 2159 3823 Size: 32 135 32 508 32 15 32 1664 Cuts at: 3823 3855 3897 3929 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1664 508 135 42 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 2080 2275 2526 2772 669 Size: 988 281 142 195 251 246 3079 Fragments arranged by size: 3079 988 281 251 246 195 142 HindIII A'AGCT_T Cuts at: 748 748 Size: 5182 HpaI GTT'AAC Cuts at: 2080 2275 2772 2080 Size: 195 497 4490 Fragments arranged by size: 4490 497 195 KpnI G_GTAC'C Cuts at: 2174 2507 2174 Size: 333 4849 MfeI C'AATT_G Cuts at: 2781 2781 Size: 5182 MluI A'CGCG_T Cuts at: 5058 5058 Size: 5182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4296 4480 1305 Size: 2991 184 2007 Fragments arranged by size: 2991 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3360 Fragments arranged by size: 3360 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 2429 3140 3499 3658 518 Size: 648 1230 33 711 359 159 2042 Fragments arranged by size: 2042 1230 711 648 359 159 33 MspA1I CmG'CkG Cuts at: 1606 2263 2598 3275 4423 4668 1606 Size: 657 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 657 335 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2206 513 Size: 552 939 202 3489 Fragments arranged by size: 3489 939 552 202 NdeI CA'TA_TG Cuts at: 387 387 Size: 5182 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5182 NotI GC'GGCC_GC Cuts at: 2629 2629 Size: 5182 NspI r_CATG'y Cuts at: 1132 1229 2540 4085 1132 Size: 97 1311 1545 2229 Fragments arranged by size: 2229 1545 1311 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5182 PciI A'CATG_T Cuts at: 4081 4081 Size: 5182 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5182 PfoI T'CCnGG_A Cuts at: 2469 2469 Size: 5182 PmeI GTTT'AAAC Cuts at: 2486 2486 Size: 5182 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3251 3283 4764 4796 3251 Size: 32 1481 32 3637 Fragments arranged by size: 3637 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5182 PsiI TTA'TAA Cuts at: 2752 2752 Size: 5182 PstI C_TGCA'G Cuts at: 830 2534 830 Size: 1704 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5182 PvuII CAG'CTG Cuts at: 2263 2263 Size: 5182 SalI G'TCGA_C Cuts at: 1936 2524 1936 Size: 588 4594 SbfI CC_TGCA'GG Cuts at: 2534 2534 Size: 5182 ScaI AGT'ACT Cuts at: 3430 5142 3430 Size: 1712 3470 SfcI C'TryA_G Cuts at: 826 1045 2530 3665 4346 4537 826 Size: 219 1485 1135 681 191 1471 Fragments arranged by size: 1485 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5182 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5182 SmaI CCC'GGG Cuts at: 1872 2509 1872 Size: 637 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3286 4187 4449 820 Size: 197 926 1343 901 262 1553 Fragments arranged by size: 1553 1343 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2973 493 Size: 2480 2702 SpeI A'CTAG_T Cuts at: 152 2113 152 Size: 1961 3221 SphI G_CATG'C Cuts at: 1132 2540 1132 Size: 1408 3774 SspI AAT'ATT Cuts at: 5 52 3106 5 Size: 47 3054 2081 Fragments arranged by size: 3054 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5182 StyI C'CwwG_G Cuts at: 513 1065 2004 2206 2621 513 Size: 552 939 202 415 3074 Fragments arranged by size: 3074 939 552 415 202 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3209 3226 3379 3564 4009 325 Size: 1255 1629 17 153 185 445 1498 Fragments arranged by size: 1629 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3428 5140 96 Size: 274 80 33 51 1443 1451 1712 138 Fragments arranged by size: 1712 1451 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5142 5174 5142 Size: 32 5150 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5182 XbaI T'CTAG_A Cuts at: 2518 2518 Size: 5182 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2260 2260 Size: 5182 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5182 XmaI C'CCGG_G Cuts at: 1870 2507 1870 Size: 637 4545 XmnI GAAnn'nnTTC Cuts at: 2090 3311 2090 Size: 1221 3961 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2990 276 Size: 53 83 186 936 1456 2468 Fragments arranged by size: 2468 1456 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI