(Circular) (Six-base) MAPSORT of: pFN21AE1031.seq Check: 7972 from: 1 to: 8884 pFN21AE1031 8884 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4102 4872 4102 Size: 770 8114 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5621 6694 278 Size: 53 83 186 936 4085 1073 2468 Fragments arranged by size: 4085 2468 1073 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6227 1937 Size: 4290 4594 Acc65I G'GTAC_C Cuts at: 3459 6205 3459 Size: 2746 6138 AclI AA'CG_TT Cuts at: 1563 7011 7384 1563 Size: 5448 373 3063 Fragments arranged by size: 5448 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2511 3071 3585 5328 5840 5856 6947 8331 Size: 879 560 514 1743 512 16 1091 1384 Cuts at: 8331 8599 1632 Size: 268 1917 Fragments arranged by size: 1917 1743 1384 1091 879 560 514 512 268 16 AfeI AGC'GCT Cuts at: 4905 4905 Size: 8884 AflII C'TTAA_G Cuts at: 820 1017 3307 820 Size: 197 2290 6397 Fragments arranged by size: 6397 2290 197 AflIII A'CryG_T Cuts at: 1203 5758 7783 8760 1203 Size: 4555 2025 977 1327 Fragments arranged by size: 4555 2025 1327 977 AgeI A'CCGG_T Cuts at: 7715 7715 Size: 8884 AhdI GACnn_n'nnGTC Cuts at: 3605 5089 7613 3605 Size: 1484 2524 4876 Fragments arranged by size: 4876 2524 1484 AleI CACnn'nnGTG Cuts at: 1166 5902 1166 Size: 4736 4148 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2328 5408 6020 1184 Size: 1144 3080 612 4048 Fragments arranged by size: 4048 3080 1144 612 AlwNI CAG_nnn'CTG Cuts at: 2834 3187 3680 4873 4915 5230 5536 6145 6627 Size: 353 493 1193 42 315 306 609 482 Cuts at: 6627 8199 2834 Size: 1572 3519 Fragments arranged by size: 3519 1572 1193 609 493 482 353 315 306 42 ApaI G_GGCC'C Cuts at: 2504 2537 3268 4382 4846 5695 2504 Size: 33 731 1114 464 849 5693 Fragments arranged by size: 5693 1114 849 731 464 33 ApaLI G'TGCA_C Cuts at: 6941 8097 6941 Size: 1156 7728 ApoI r'AATT_y Cuts at: 1493 6193 6419 6571 8680 1493 Size: 4700 226 152 2109 1697 Fragments arranged by size: 4700 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7438 160 Size: 872 6406 1606 Fragments arranged by size: 6406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8884 AvaI C'yCGr_G Cuts at: 1870 1943 4630 4768 5202 5356 6209 1870 Size: 73 2687 138 434 154 853 4545 Fragments arranged by size: 4545 2687 853 434 154 138 73 AvrII C'CTAG_G Cuts at: 2573 2573 Size: 8884 BamHI G'GATC_C Cuts at: 6214 6581 6214 Size: 367 8517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2546 3459 4117 5430 5904 Size: 325 217 627 759 913 658 1313 474 Cuts at: 5904 6205 7660 618 Size: 301 1455 1842 Fragments arranged by size: 1842 1455 1313 913 759 658 627 474 325 301 217 BanII G_rGCy'C Cuts at: 1391 2504 2537 2649 2913 3268 3409 3438 3621 Size: 1113 33 112 264 355 141 29 183 Cuts at: 3621 4086 4131 4305 4382 4846 5695 5779 5975 Size: 465 45 174 77 464 849 84 196 Cuts at: 5975 6102 6203 1391 Size: 127 101 4072 Fragments arranged by size: 4072 1113 849 465 464 355 264 196 183 174 141 127 112 101 84 77 45 33 29 BbeI G_GCGC'C Cuts at: 5434 5434 Size: 8884 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3077 5844 928 Size: 971 1178 2767 3968 Fragments arranged by size: 3968 2767 1178 971 BbvCI CC'TCA_GC Cuts at: 2288 3892 3966 4248 2288 Size: 1604 74 282 6924 Fragments arranged by size: 6924 1604 282 74 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 7075 7109 1903 Size: 34 50 34 5054 34 3678 Fragments arranged by size: 5054 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6776 7986 6776 Size: 1210 7674 BclI T'GATC_A Cuts at: 1548 2299 1548 Size: 751 8133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4760 5225 5471 Size: 107 122 71 631 605 3088 465 246 Cuts at: 5471 7495 136 Size: 2024 1525 Fragments arranged by size: 3088 2024 1525 631 605 465 246 122 107 71 BglII A'GATC_T Cuts at: 2792 8879 2792 Size: 6087 2797 BlpI GC'TnA_GC Cuts at: 2664 4013 4130 4663 4964 5012 6301 2664 Size: 1349 117 533 301 48 1289 5247 Fragments arranged by size: 5247 1349 1289 533 301 117 48 Bme1580I G_kGCm'C Cuts at: 1790 2376 2504 2526 2537 3268 3764 4382 4389 Size: 586 128 22 11 731 496 618 7 Cuts at: 4389 4846 5387 5457 5695 5909 6945 8101 1790 Size: 457 541 70 238 214 1036 1156 2573 Fragments arranged by size: 2573 1156 1036 731 618 586 541 496 457 238 214 128 70 22 11 7 BmgBI CAC'GTC Cuts at: 1318 3112 1318 Size: 1794 7090 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2370 4085 4376 Size: 416 53 83 399 24 953 1715 291 Cuts at: 4376 4535 4631 5519 5791 6615 7573 442 Size: 159 96 888 272 824 958 1753 Fragments arranged by size: 1753 1715 958 953 888 824 416 399 291 272 159 96 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3296 1056 Size: 2240 6644 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2848 5683 5805 2848 Size: 2835 122 5927 Fragments arranged by size: 5927 2835 122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2951 3026 3444 3680 3776 4289 4450 Size: 899 813 75 418 236 96 513 161 Cuts at: 4450 4451 4760 4775 5051 5834 6122 7544 1239 Size: 1 309 15 276 783 288 1422 2579 Fragments arranged by size: 2579 1422 899 813 783 513 418 309 288 276 236 161 96 75 15 1 Bpu10I CC'TnA_GC Cuts at: 2288 2648 3892 3966 4248 5142 2288 Size: 360 1244 74 282 894 6030 Fragments arranged by size: 6030 1244 894 360 282 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2809 5556 7009 7874 8172 2809 Size: 2747 1453 865 298 3521 Fragments arranged by size: 3521 2747 1453 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2008 3816 6044 6173 7547 882 Size: 617 509 1808 2228 129 1374 2219 Fragments arranged by size: 2228 2219 1808 1374 617 509 129 BsaAI yAC'GTr Cuts at: 493 1204 3655 5761 6675 493 Size: 711 2451 2106 914 2702 Fragments arranged by size: 2702 2451 2106 914 711 BsaBI GATnn'nnATC Cuts at: 8878 8878 Size: 8884 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5431 5618 6691 7073 Size: 53 83 186 936 3898 187 1073 382 Cuts at: 7073 7708 275 Size: 635 1451 Fragments arranged by size: 3898 1451 1073 936 635 382 187 186 83 53 BsaWI w'CCGG_w Cuts at: 3939 5868 7317 7715 7989 8136 8547 3939 Size: 1929 1449 398 274 147 411 4276 Fragments arranged by size: 4276 1929 1449 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2554 2584 4469 4499 5815 Size: 30 165 30 1151 30 1885 30 1316 Cuts at: 5815 5845 1178 Size: 30 4217 Fragments arranged by size: 4217 1885 1316 1151 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2211 2305 2741 2852 3167 3428 3446 4178 Size: 1025 94 436 111 315 261 18 732 Cuts at: 4178 4505 4685 5540 1186 Size: 327 180 855 4530 Fragments arranged by size: 4530 1025 855 732 436 327 315 261 180 111 94 18 BseYI C'CCAG_C Cuts at: 2086 2203 2240 2760 2771 2944 3216 3491 3535 Size: 117 37 520 11 173 272 275 44 Cuts at: 3535 3896 4194 4607 5162 5556 8087 8583 2086 Size: 361 298 413 555 394 2531 496 2387 Fragments arranged by size: 2531 2387 555 520 496 413 394 361 298 275 272 173 117 44 37 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 2100 2114 2969 3122 5216 6041 Size: 573 297 67 14 855 153 2094 825 Cuts at: 6041 1163 Size: 4006 Fragments arranged by size: 4006 2094 855 825 573 297 153 67 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6334 7095 8123 1540 Size: 275 186 4333 761 1028 2301 Fragments arranged by size: 4333 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3229 3400 3438 3711 4019 4086 4131 5779 5916 Size: 171 38 273 308 67 45 1648 137 Cuts at: 5916 5975 6102 6162 6945 7030 8101 3229 Size: 59 127 60 783 85 1071 4012 Fragments arranged by size: 4012 1648 1071 783 308 273 171 137 127 85 67 60 59 45 38 BsmI GAATG_Cn' Cuts at: 3536 3727 6395 6488 3536 Size: 191 2668 93 5932 Fragments arranged by size: 5932 2668 191 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4652 4652 Size: 8884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2376 2504 2526 2537 2649 2913 3229 Size: 399 586 128 22 11 112 264 316 Cuts at: 3229 3268 3400 3409 3438 3621 3711 3764 4019 Size: 39 132 9 29 183 90 53 255 Cuts at: 4019 4086 4131 4305 4382 4389 4846 5387 5457 Size: 67 45 174 77 7 457 541 70 Cuts at: 5457 5695 5779 5909 5916 5975 6102 6162 6203 Size: 238 84 130 7 59 127 60 41 Cuts at: 6203 6945 7030 8101 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 586 541 457 399 316 264 255 238 183 174 132 130 128 127 112 90 85 84 77 70 67 60 59 53 45 41 39 29 22 11 9 7 7 BspEI T'CCGG_A Cuts at: 3939 5868 8547 3939 Size: 1929 2679 4276 Fragments arranged by size: 4276 2679 1929 BspHI T'CATG_A Cuts at: 6773 8503 6773 Size: 1730 7154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 3126 3688 4102 4872 6239 844 Size: 1208 1074 562 414 770 1367 3489 Fragments arranged by size: 3489 1367 1208 1074 770 562 414 BsrBI CCG'CTC Cuts at: 1394 1823 2152 3167 6771 1394 Size: 429 329 1015 3604 3507 Fragments arranged by size: 3604 3507 1015 429 329 BsrDI GCAATG_nn' Cuts at: 65 1248 2954 3378 5188 7379 7553 65 Size: 1183 1706 424 1810 2191 174 1396 Fragments arranged by size: 2191 1810 1706 1396 1183 424 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2142 3288 3576 4211 4968 7528 7715 Size: 110 332 1146 288 635 757 2560 187 Cuts at: 7715 1700 Size: 2869 Fragments arranged by size: 2869 2560 1146 757 635 332 288 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8884 BssSI C'ACGA_G Cuts at: 4613 6944 7956 4613 Size: 2331 1012 5541 Fragments arranged by size: 5541 2331 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2078 2752 4117 4915 5410 6055 794 Size: 1284 674 1365 798 495 645 3623 Fragments arranged by size: 3623 1365 1284 798 674 645 495 BstBI TT'CG_AA Cuts at: 7688 7688 Size: 8884 BstEII G'GTnAC_C Cuts at: 1184 4067 1184 Size: 2883 6001 BstXI CCAn_nnnn'nTGG Cuts at: 2597 3130 5215 5647 5687 2597 Size: 533 2085 432 40 5794 Fragments arranged by size: 5794 2085 533 432 40 BstYI r'GATC_y Cuts at: 1972 2730 2792 4722 6214 6581 6966 6983 8435 Size: 758 62 1930 1492 367 385 17 1452 Cuts at: 8435 8521 8533 8879 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1930 1492 1452 758 385 367 346 86 62 17 12 Bsu36I CC'TnA_GG Cuts at: 2262 2724 4547 2262 Size: 462 1823 6599 Fragments arranged by size: 6599 1823 462 BtgI C'CryG_G Cuts at: 513 1065 2715 3145 3463 4121 4374 5379 513 Size: 552 1650 430 318 658 253 1005 4018 Fragments arranged by size: 4018 1650 1005 658 552 430 318 253 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8145 Fragments arranged by size: 8145 683 56 BtsI GCAGTG_nn' Cuts at: 2097 2251 2310 3233 3307 4175 4505 4552 4686 Size: 154 59 923 74 868 330 47 134 Cuts at: 4686 4913 5411 6400 7194 7214 2097 Size: 227 498 989 794 20 3767 Fragments arranged by size: 3767 989 923 868 794 498 330 227 154 134 74 59 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2334 2369 529 Size: 35 1770 35 7044 Fragments arranged by size: 7044 1770 35 35 DraI TTT'AAA Cuts at: 6188 6188 Size: 8884 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4294 7891 809 Size: 507 2978 3597 1802 Fragments arranged by size: 3597 2978 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2248 2355 4057 Size: 54 1425 50 275 18 418 107 1702 Cuts at: 4057 4263 4741 6331 7220 8849 8 Size: 206 478 1590 889 1629 43 Fragments arranged by size: 1702 1629 1590 1425 889 478 418 275 206 107 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6331 1537 Size: 275 4519 4090 Fragments arranged by size: 4519 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2274 2685 2912 3341 3405 4707 6814 1353 Size: 921 411 227 429 64 1302 2107 3423 Fragments arranged by size: 3423 2107 1302 921 429 411 227 64 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3704 5882 7471 7845 7991 387 Size: 1064 138 2115 2178 1589 374 146 1280 Fragments arranged by size: 2178 2115 1589 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3436 4084 4129 5777 5973 6100 3436 Size: 648 45 1648 196 127 6220 Fragments arranged by size: 6220 1648 648 196 127 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2511 2951 3026 3071 3444 3585 Size: 393 506 373 440 75 45 373 141 Cuts at: 3585 3680 3776 4289 4450 4451 4760 4775 5051 Size: 95 96 513 161 1 309 15 276 Cuts at: 5051 5328 5834 5840 5856 6122 6947 7544 8331 Size: 277 506 6 16 266 825 597 787 Cuts at: 8331 8599 1239 Size: 268 1524 Fragments arranged by size: 1524 825 787 597 513 506 506 440 393 373 373 309 277 276 268 266 161 141 96 95 75 45 16 15 6 1 EcoNI CCTnn'n_nnAGG Cuts at: 2261 4487 2261 Size: 2226 6658 EcoO109I rG'GnC_Cy Cuts at: 2272 2500 2621 2855 3264 3677 3751 3827 4024 Size: 228 121 234 409 413 74 76 197 Cuts at: 4024 4843 5155 5398 5691 5692 5962 2272 Size: 819 312 243 293 1 270 5194 Fragments arranged by size: 5194 819 413 409 312 293 270 243 234 228 197 121 76 74 1 EcoRI G'AATT_C Cuts at: 6193 6571 6193 Size: 378 8506 FspI TGC'GCA Cuts at: 7390 7390 Size: 8884 HaeII r_GCGC'y Cuts at: 1678 4483 4907 5434 8031 1678 Size: 2805 424 527 2597 2531 Fragments arranged by size: 2805 2597 2531 527 424 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2284 2316 2554 Size: 32 135 32 548 32 132 32 238 Cuts at: 2554 2586 3058 3090 3466 3498 3591 3623 4469 Size: 32 472 32 376 32 93 32 846 Cuts at: 4469 4501 5075 5107 5339 5371 7525 7557 7599 Size: 32 574 32 232 32 2154 32 42 Cuts at: 7599 7631 1373 Size: 32 2626 Fragments arranged by size: 2626 2154 846 574 548 472 376 238 232 135 132 93 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6228 6474 669 Size: 988 281 4290 246 3079 Fragments arranged by size: 4290 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 6065 748 Size: 5317 3567 HpaI GTT'AAC Cuts at: 6474 6474 Size: 8884 KasI G'GCGC_C Cuts at: 5430 5430 Size: 8884 KpnI G_GTAC'C Cuts at: 3463 6209 3463 Size: 2746 6138 MfeI C'AATT_G Cuts at: 6483 6483 Size: 8884 MluI A'CGCG_T Cuts at: 8760 8760 Size: 8884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2160 7998 8182 1305 Size: 855 5838 184 2007 Fragments arranged by size: 5838 2007 855 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2250 4059 4743 10 Size: 54 1425 343 418 1809 684 4151 Fragments arranged by size: 4151 1809 1425 684 418 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2166 3144 5390 5486 5902 6842 7201 Size: 648 1000 978 2246 96 416 940 359 Cuts at: 7201 7360 518 Size: 159 2042 Fragments arranged by size: 2246 2042 1000 978 940 648 416 359 159 96 MspA1I CmG'CkG Cuts at: 1606 2075 2098 2749 3896 4123 4165 4576 4747 Size: 469 23 651 1147 227 42 411 171 Cuts at: 4747 5089 5290 6300 6977 8125 8370 1606 Size: 342 201 1010 677 1148 245 2120 Fragments arranged by size: 2120 1148 1147 1010 677 651 469 411 342 245 227 201 171 42 23 NaeI GCC'GGC Cuts at: 3578 4213 4970 3578 Size: 635 757 7492 Fragments arranged by size: 7492 757 635 NarI GG'CG_CC Cuts at: 5431 5431 Size: 8884 NcoI C'CATG_G Cuts at: 513 1065 2715 3145 3463 5379 513 Size: 552 1650 430 318 1916 4018 Fragments arranged by size: 4018 1916 1650 552 430 318 NdeI CA'TA_TG Cuts at: 387 387 Size: 8884 NgoMIV G'CCGG_C Cuts at: 3576 4211 4968 3576 Size: 635 757 7492 Fragments arranged by size: 7492 757 635 NheI G'CTAG_C Cuts at: 1052 3292 1052 Size: 2240 6644 NotI GC'GGCC_GC Cuts at: 6331 6331 Size: 8884 NspI r_CATG'y Cuts at: 1132 1229 3242 6242 7787 1132 Size: 97 2013 3000 1545 2229 Fragments arranged by size: 3000 2229 2013 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8884 PciI A'CATG_T Cuts at: 7783 7783 Size: 8884 PflMI CCAn_nnn'nTGG Cuts at: 1108 2510 3807 3982 4474 4798 5304 1108 Size: 1402 1297 175 492 324 506 4688 Fragments arranged by size: 4688 1402 1297 506 492 324 175 PfoI T'CCnGG_A Cuts at: 2154 3421 3753 4467 5040 5514 5646 2154 Size: 1267 332 714 573 474 132 5392 Fragments arranged by size: 5392 1267 714 573 474 332 132 PmeI GTTT'AAAC Cuts at: 6188 6188 Size: 8884 PmlI CAC'GTG Cuts at: 5761 5761 Size: 8884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6953 6985 8466 8498 6953 Size: 32 1481 32 7339 Fragments arranged by size: 7339 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2272 2621 2855 3677 3751 5155 5398 5962 2272 Size: 349 234 822 74 1404 243 564 5194 Fragments arranged by size: 5194 1404 822 564 349 243 234 74 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8884 PsiI TTA'TAA Cuts at: 6454 6454 Size: 8884 PspOMI G'GGCC_C Cuts at: 2500 2533 3264 4378 4842 5691 2500 Size: 33 731 1114 464 849 5693 Fragments arranged by size: 5693 1114 849 731 464 33 PstI C_TGCA'G Cuts at: 830 2712 2739 4869 4911 5406 5715 6012 6236 Size: 1882 27 2130 42 495 309 297 224 Cuts at: 6236 830 Size: 3478 Fragments arranged by size: 3478 2130 1882 495 309 297 224 42 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8884 PvuII CAG'CTG Cuts at: 2075 2098 3896 4747 5089 2075 Size: 23 1798 851 342 5870 Fragments arranged by size: 5870 1798 851 342 23 SacI G_AGCT'C Cuts at: 3438 4086 4131 5779 5975 6102 3438 Size: 648 45 1648 196 127 6220 Fragments arranged by size: 6220 1648 648 196 127 45 SacII CC_GC'GG Cuts at: 4124 4124 Size: 8884 SalI G'TCGA_C Cuts at: 1936 6226 1936 Size: 4290 4594 SanDI GG'GwC_CC Cuts at: 2621 2621 Size: 8884 SapI GCTCTTCn'nnn_ Cuts at: 3405 3405 Size: 8884 SbfI CC_TGCA'GG Cuts at: 2739 6236 2739 Size: 3497 5387 ScaI AGT'ACT Cuts at: 7132 8844 7132 Size: 1712 7172 SfcI C'TryA_G Cuts at: 826 1045 2708 2735 3179 3780 4865 4907 5246 Size: 219 1663 27 444 601 1085 42 339 Cuts at: 5246 5402 5711 6008 6232 7367 8048 8239 826 Size: 156 309 297 224 1135 681 191 1471 Fragments arranged by size: 1663 1471 1135 1085 681 601 444 339 309 297 224 219 191 156 42 27 SfoI GGC'GCC Cuts at: 5432 5432 Size: 8884 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8884 SmaI CCC'GGG Cuts at: 1872 4770 6211 1872 Size: 2898 1441 4545 Fragments arranged by size: 4545 2898 1441 SmlI C'TyrA_G Cuts at: 820 1017 1943 2788 3307 5571 6988 7889 8151 Size: 197 926 845 519 2264 1417 901 262 Cuts at: 8151 820 Size: 1553 Fragments arranged by size: 2264 1553 1417 926 901 845 519 262 197 SnaBI TAC'GTA Cuts at: 493 6675 493 Size: 6182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8884 SphI G_CATG'C Cuts at: 1132 6242 1132 Size: 5110 3774 SspI AAT'ATT Cuts at: 5 52 3907 6808 5 Size: 47 3855 2901 2081 Fragments arranged by size: 3855 2901 2081 47 StuI AGG'CCT Cuts at: 1517 4427 5147 1517 Size: 2910 720 5254 Fragments arranged by size: 5254 2910 720 StyI C'CwwG_G Cuts at: 513 1065 2496 2573 2715 2874 3145 3354 3463 Size: 552 1431 77 142 159 271 209 109 Cuts at: 3463 4953 5379 6323 513 Size: 1490 426 944 3074 Fragments arranged by size: 3074 1490 1431 944 552 426 271 209 159 142 109 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2032 2581 3140 4151 4155 4208 4940 Size: 1255 452 549 559 1011 4 53 732 Cuts at: 4940 5012 5398 6911 6928 7081 7266 7711 325 Size: 72 386 1513 17 153 185 445 1498 Fragments arranged by size: 1513 1498 1255 1011 732 559 549 452 445 386 185 153 72 53 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5527 7130 8842 Size: 274 80 33 51 1443 3550 1603 1712 Cuts at: 8842 96 Size: 138 Fragments arranged by size: 3550 1712 1603 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3669 3701 5815 5847 8844 8876 3669 Size: 32 2114 32 2997 32 3677 Fragments arranged by size: 3677 2997 2114 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2996 1316 Size: 1680 7204 XbaI T'CTAG_A Cuts at: 6220 6220 Size: 8884 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3751 5377 3751 Size: 1626 7258 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8884 XmaI C'CCGG_G Cuts at: 1870 4768 6209 1870 Size: 2898 1441 4545 Fragments arranged by size: 4545 2898 1441 XmnI GAAnn'nnTTC Cuts at: 4784 7013 4784 Size: 2229 6655 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5619 6692 276 Size: 53 83 186 936 4085 1073 2468 Fragments arranged by size: 4085 2468 1073 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI SrfI SwaI