Restriction map for
pFN21AE1030
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AvaII AviII AvrII BanII
BbeI BbrPI BbvCI BciVI BcnI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI Bsu36I CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HpaII KasI
KspI MamI MfeI MluI MroI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 555 GGTACC G^GTACC
Asp718I 555 GGTACC G^GTACC
AspI 3795 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 242 CTCGAG C^[CT]CG[GA]G
BalI 1568 TGGCCA TGG^CCA
BamHI 785 GGATCC G^GATCC
BanI 555 GGTACC G^G[CT][GA]CC
BcgI 472 GCAGCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 3028 GGTCTC GGTCTC(1/5)
BsoBI 242 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2895 TGTACA T^GTACA
BsrGI 2895 TGTACA T^GTACA
Bst1107I 1280 GTATAC GTA^TAC
BstXI 789 CCAGAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1280 GTATAC GTA^TAC
Cfr13I 1965 GGCCC G^G[ACGT]CC
DraII 1964 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 1568 TGGCCA [CT]^GGCC[GA]
EcoO109I 1964 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1616 CCTTGG C^C[AT][AT]GG
EcoT22I 2205 ATGCAT ATGCA^T
HgaI 400 GACGC GACGC(5/10)
HincII 1071 GTTAAC GT[CT]^[GA]AC
HindII 1071 GTTAAC GT[CT]^[GA]AC
HpaI 1071 GTTAAC GTT^AAC
KpnI 555 GGTACC GGTAC^C
MaeII 1878 ACGT A^CGT
MluNI 1568 TGGCCA TGG^CCA
MscI 1568 TGGCCA TGG^CCA
NsiI 2205 ATGCAT ATGCA^T
PaeR7I 242 CTCGAG C^TCGAG
PflFI 3795 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 721 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1334 CTGCAG CTGCA^G
Sau96I 1965 GGCCC G^G[ACGT]CC
SexAI 3887 ACCAGGT A^CC[AT]GGT
SspBI 2895 TGTACA T^GTACA
StuI 2762 AGGCCT AGG^CCT
StyI 1616 CCTTGG C^C[AT][AT]GG
TspRI 3695 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 3795 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3264 TCTAGA T^CTAGA
XcmI 2409 CCATGAGAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 242 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 913 GTAGAC GT^[AC][GT]AC
1280 GTATAC
AflII 1909 CTTAAG C^TTAAG
2395 CTTAAG
AflIII 1182 ACATGT A^C[GA][CT]GT
2727 ACATGT
Asp700I 1492 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2440 GAAGAGCTTC
AspHI 3268 GAGCAC G[AT]GC[AT]^C
3880 GAGCAC
BclI 282 TGATCA T^GATCA
2361 TGATCA
BfrI 1909 CTTAAG C^TTAAG
2395 CTTAAG
BlpI 1435 GCTGAGC GC^T[ACGT]AGC
2350 GCTAAGC
BmyI 3268 GAGCAC G[AGT]GC[ACT]^C
3880 GAGCAC
Bpu1102I 1435 GCTGAGC GC^T[ACGT]AGC
2350 GCTAAGC
BseRI 2287 GAGGAG GAGGAG(10/8)
3250 GAGGAG
BsgI 1991 CTGCAC GTGCAG(16/14)
1951 GTGCAG
BsiHKAI 3268 GAGCAC G[AT]GC[AT]^C
3880 GAGCAC
BsiYI 2672 CCAATACAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3768 CCCAGAAACGG
BslI 2672 CCAATACAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3768 CCCAGAAACGG
Bsp1286I 3268 GAGCAC G[AGT]GC[ACT]^C
3880 GAGCAC
BspHI 83 TCATGA T^CATGA
2979 TCATGA
BspLU11I 1182 ACATGT A^CATGT
2727 ACATGT
BspMI 381 GCAGGT ACCTGC(4/8)
1707 GCAGGT
BsrDI 578 CATTGC GCAATG(2/0)
1106 GCAATG
BstYI 785 GGATCC [GA]^GATC[CT]
2694 GGATCT
CelII 1435 GCTGAGC GC^T[ACGT]AGC
2350 GCTAAGC
FbaI 282 TGATCA T^GATCA
2361 TGATCA
MflI 785 GGATCC [GA]^GATC[CT]
2694 GGATCT
NlaIV 555 GGTACC GG[ACGT]^[ACGT]CC
785 GGATCC
NspI 1182 ACATGT [GA]CATG^[CT]
2727 ACATGT
PvuII 473 CAGCTG CAG^CTG
2881 CAGCTG
RcaI 83 TCATGA T^CATGA
2979 TCATGA
Tsp45I 398 GTGAC ^GT[GC]AC
3693 GTCAC
XhoII 785 GGATCC [GA]^GATC[CT]
2694 GGATCT
XmnI 1492 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2440 GAAGAGCTTC
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BbsI BbvI BfaI
BpmI BpuAI BsaJI BsmAI BsmFI
BsmI BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
PleI RsaI SapI Sau3AI ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp509I TthHB8I