(Circular) (Six-base) MAPSORT of: pFN21AE1030.seq Check: 4773 from: 1 to: 8605 pFN21AE1030 8605 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6415 278 Size: 53 83 186 936 4879 2468 Fragments arranged by size: 4879 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2919 3286 5948 1937 Size: 982 367 2662 4594 Fragments arranged by size: 4594 2662 982 367 Acc65I G'GTAC_C Cuts at: 2560 5926 2560 Size: 3366 5239 AclI AA'CG_TT Cuts at: 1563 6732 7105 1563 Size: 5169 373 3063 Fragments arranged by size: 5169 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2493 2837 2865 2916 3783 3882 3972 4583 Size: 861 344 28 51 867 99 90 611 Cuts at: 4583 5229 5454 5834 6668 8052 8320 1632 Size: 646 225 380 834 1384 268 1917 Fragments arranged by size: 1917 1384 867 861 834 646 611 380 344 268 225 99 90 51 28 AflII C'TTAA_G Cuts at: 820 1017 3914 4400 820 Size: 197 2897 486 5025 Fragments arranged by size: 5025 2897 486 197 AflIII A'CryG_T Cuts at: 1203 3187 4732 7504 8481 1203 Size: 1984 1545 2772 977 1327 Fragments arranged by size: 2772 1984 1545 1327 977 AgeI A'CCGG_T Cuts at: 7436 7436 Size: 8605 AhdI GACnn_n'nnGTC Cuts at: 7334 7334 Size: 8605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4008 1184 Size: 2824 5781 AlwNI CAG_nnn'CTG Cuts at: 3598 3973 5701 6348 7920 3598 Size: 375 1728 647 1572 4283 Fragments arranged by size: 4283 1728 1572 647 375 ApaLI G'TGCA_C Cuts at: 6662 7818 6662 Size: 1156 7449 ApoI r'AATT_y Cuts at: 1493 3566 3756 3934 4080 4100 4318 5361 5914 Size: 2073 190 178 146 20 218 1043 553 Cuts at: 5914 6140 6292 8401 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 2073 1697 1043 553 226 218 190 178 152 146 20 AseI AT'TA_AT Cuts at: 160 1032 7159 160 Size: 872 6127 1606 Fragments arranged by size: 6127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8605 AvaI C'yCGr_G Cuts at: 1870 1943 2247 5930 1870 Size: 73 304 3683 4545 Fragments arranged by size: 4545 3683 304 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3609 3642 3609 Size: 33 8572 BamHI G'GATC_C Cuts at: 2790 5935 6302 2790 Size: 3145 367 5093 Fragments arranged by size: 5093 3145 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2560 5926 7381 618 Size: 325 217 627 773 3366 1455 1842 Fragments arranged by size: 3366 1842 1455 773 627 325 217 BanII G_rGCy'C Cuts at: 1391 5924 1391 Size: 4533 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2659 2825 2885 5778 928 Size: 971 760 166 60 2893 3755 Fragments arranged by size: 3755 2893 971 760 166 60 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2466 2500 6796 6830 1903 Size: 34 529 34 4296 34 3678 Fragments arranged by size: 4296 3678 529 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6497 7707 6497 Size: 1210 7395 BclI T'GATC_A Cuts at: 1548 2287 4366 1548 Size: 739 2079 5787 Fragments arranged by size: 5787 2079 739 BfrBI ATG'CAT Cuts at: 4212 4212 Size: 8605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7216 136 Size: 107 122 71 631 605 5544 1525 Fragments arranged by size: 5544 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8600 8600 Size: 8605 BlpI GC'TnA_GC Cuts at: 3441 4356 6022 3441 Size: 915 1666 6024 Fragments arranged by size: 6024 1666 915 Bme1580I G_kGCm'C Cuts at: 1790 6666 7822 1790 Size: 4876 1156 2573 Fragments arranged by size: 4876 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2438 6336 7294 Size: 416 53 83 399 24 1021 3898 958 Cuts at: 7294 442 Size: 1753 Fragments arranged by size: 3898 1753 1021 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8605 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2262 2262 Size: 8605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4475 5723 5757 7265 1239 Size: 3236 1248 34 1508 2579 Fragments arranged by size: 3236 2579 1508 1248 34 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2713 4817 6730 7595 7893 2713 Size: 2104 1913 865 298 3425 Fragments arranged by size: 3425 2104 1913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5039 7268 882 Size: 617 3540 2229 2219 Fragments arranged by size: 3540 2229 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6396 493 Size: 711 5192 2702 Fragments arranged by size: 5192 2702 711 BsaBI GATnn'nnATC Cuts at: 8599 8599 Size: 8605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6412 6794 7429 275 Size: 53 83 186 936 4879 382 635 1451 Fragments arranged by size: 4879 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7038 7436 7710 7857 8268 7038 Size: 398 274 147 411 7375 Fragments arranged by size: 7375 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5789 5819 1178 Size: 30 165 30 4386 30 3964 Fragments arranged by size: 4386 3964 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4307 5270 1186 Size: 3121 963 4521 Fragments arranged by size: 4521 3121 963 BseYI C'CCAG_C Cuts at: 7808 8304 7808 Size: 496 8109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3977 3981 1163 Size: 573 2241 4 5787 Fragments arranged by size: 5787 2241 573 4 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6055 6816 7844 1540 Size: 275 186 4054 761 1028 2301 Fragments arranged by size: 4054 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5277 5889 6666 6751 7822 5277 Size: 612 777 85 1071 6060 Fragments arranged by size: 6060 1071 777 612 85 BsmI GAATG_Cn' Cuts at: 3442 4212 4306 6116 6209 3442 Size: 770 94 1810 93 5838 Fragments arranged by size: 5838 1810 770 94 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 5277 5889 5924 6666 6751 7822 1391 Size: 399 3487 612 35 742 85 1071 2174 Fragments arranged by size: 3487 2174 1071 742 612 399 85 35 BspEI T'CCGG_A Cuts at: 8268 8268 Size: 8605 BspHI T'CATG_A Cuts at: 2088 4984 6494 8224 2088 Size: 2896 1510 1730 2469 Fragments arranged by size: 2896 2469 1730 1510 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2377 3703 5960 844 Size: 1533 1326 2257 3489 Fragments arranged by size: 3489 2257 1533 1326 BsrBI CCG'CTC Cuts at: 1394 1823 6492 1394 Size: 429 4669 3507 Fragments arranged by size: 4669 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2582 3118 7100 7274 65 Size: 1183 1334 536 3982 174 1396 Fragments arranged by size: 3982 1396 1334 1183 536 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7249 7436 1700 Size: 110 5439 187 2869 Fragments arranged by size: 5439 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4900 96 Size: 4804 3801 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8605 BssSI C'ACGA_G Cuts at: 6665 7677 6665 Size: 1012 7593 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8605 BstBI TT'CG_AA Cuts at: 7409 7409 Size: 8605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8605 BstXI CCAn_nnnn'nTGG Cuts at: 2801 2801 Size: 8605 BstYI r'GATC_y Cuts at: 1972 2790 4699 5935 6302 6687 6704 8156 8242 Size: 818 1909 1236 367 385 17 1452 86 Cuts at: 8242 8254 8600 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1909 1452 1236 818 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3287 3287 Size: 8605 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 7114 Fragments arranged by size: 7114 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2541 487 Size: 683 56 1315 6551 Fragments arranged by size: 6551 1315 683 56 BtsI GCAGTG_nn' Cuts at: 6121 6915 6935 6121 Size: 794 20 7791 Fragments arranged by size: 7791 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2718 2753 529 Size: 35 2154 35 6381 Fragments arranged by size: 6381 2154 35 35 DraI TTT'AAA Cuts at: 2257 2857 2961 4725 5909 2257 Size: 600 104 1764 1184 4953 Fragments arranged by size: 4953 1764 1184 600 104 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7612 809 Size: 507 6296 1802 Fragments arranged by size: 6296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3573 6052 6941 Size: 54 1425 50 275 18 1743 2479 889 Cuts at: 6941 8570 8 Size: 1629 43 Fragments arranged by size: 2479 1743 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6052 1537 Size: 275 4240 4090 Fragments arranged by size: 4240 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2078 2376 3515 3690 3900 4056 4284 4440 Size: 725 298 1139 175 210 156 228 156 Cuts at: 4440 4650 4777 4826 5521 6535 1353 Size: 210 127 49 695 1014 3423 Fragments arranged by size: 3423 1139 1014 725 695 298 228 210 210 175 156 156 127 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7192 7566 7712 387 Size: 1064 138 5603 374 146 1280 Fragments arranged by size: 5603 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2493 2837 2865 2916 3783 3882 3972 Size: 393 861 344 28 51 867 99 90 Cuts at: 3972 4475 4583 5229 5454 5723 5757 5834 6668 Size: 503 108 646 225 269 34 77 834 Cuts at: 6668 7265 8052 8320 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 867 861 834 787 646 597 503 393 344 269 268 225 108 99 90 77 51 34 28 EcoO109I rG'GnC_Cy Cuts at: 3970 3970 Size: 8605 EcoRI G'AATT_C Cuts at: 5914 6292 5914 Size: 378 8227 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2882 3929 4271 2882 Size: 1047 342 7216 Fragments arranged by size: 7216 1047 342 FspI TGC'GCA Cuts at: 7111 7111 Size: 8605 HaeII r_GCGC'y Cuts at: 1678 7752 1678 Size: 6074 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3121 3153 3285 3317 5789 Size: 32 135 32 1549 32 132 32 2472 Cuts at: 5789 5821 7246 7278 7320 7352 1373 Size: 32 1425 32 42 32 2626 Fragments arranged by size: 2626 2472 1549 1425 135 132 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 5949 6195 669 Size: 988 281 1140 2871 246 3079 Fragments arranged by size: 3079 2871 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 2141 2634 3608 748 Size: 1393 493 974 5745 Fragments arranged by size: 5745 1393 974 493 HpaI GTT'AAC Cuts at: 3078 6195 3078 Size: 3117 5488 KpnI G_GTAC'C Cuts at: 2564 5930 2564 Size: 3366 5239 MfeI C'AATT_G Cuts at: 6204 6204 Size: 8605 MluI A'CGCG_T Cuts at: 8481 8481 Size: 8605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2253 3621 5106 7719 7903 1305 Size: 948 1368 1485 2613 184 2007 Fragments arranged by size: 2613 2007 1485 1368 948 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3575 10 Size: 54 1425 343 1743 5040 Fragments arranged by size: 5040 1743 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 6563 6922 7081 518 Size: 648 5397 359 159 2042 Fragments arranged by size: 5397 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2480 3530 4888 6021 6698 7846 8091 1606 Size: 874 1050 1358 1133 677 1148 245 2120 Fragments arranged by size: 2120 1358 1148 1133 1050 874 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7114 Fragments arranged by size: 7114 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8605 NotI GC'GGCC_GC Cuts at: 6052 6052 Size: 8605 NsiI A_TGCA'T Cuts at: 4214 4214 Size: 8605 NspI r_CATG'y Cuts at: 1132 1229 3191 4736 5963 7508 1132 Size: 97 1962 1545 1227 1545 2229 Fragments arranged by size: 2229 1962 1545 1545 1227 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8605 PciI A'CATG_T Cuts at: 3187 4732 7504 3187 Size: 1545 2772 4288 Fragments arranged by size: 4288 2772 1545 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8605 PmeI GTTT'AAAC Cuts at: 5909 5909 Size: 8605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6674 6706 8187 8219 6674 Size: 32 1481 32 7060 Fragments arranged by size: 7060 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2730 1613 Size: 1117 7488 PsiI TTA'TAA Cuts at: 3122 6175 3122 Size: 3053 5552 PstI C_TGCA'G Cuts at: 830 3343 5957 830 Size: 2513 2614 3478 Fragments arranged by size: 3478 2614 2513 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8605 PvuII CAG'CTG Cuts at: 2480 4888 2480 Size: 2408 6197 SalI G'TCGA_C Cuts at: 1936 5947 1936 Size: 4011 4594 SapI GCTCTTCn'nnn_ Cuts at: 2376 4056 4440 2376 Size: 1680 384 6541 Fragments arranged by size: 6541 1680 384 SbfI CC_TGCA'GG Cuts at: 5957 5957 Size: 8605 ScaI AGT'ACT Cuts at: 6853 8565 6853 Size: 1712 6893 SexAI A'CCwGG_T Cuts at: 5892 5892 Size: 8605 SfcI C'TryA_G Cuts at: 826 1045 3339 3410 4154 4440 5375 5953 7088 Size: 219 2294 71 744 286 935 578 1135 Cuts at: 7088 7769 7960 826 Size: 681 191 1471 Fragments arranged by size: 2294 1471 1135 935 744 681 578 286 219 191 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8605 SmaI CCC'GGG Cuts at: 1872 5932 1872 Size: 4060 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2247 2728 3914 4400 4796 6709 Size: 197 926 304 481 1186 486 396 1913 Cuts at: 6709 7610 7872 820 Size: 901 262 1553 Fragments arranged by size: 1913 1553 1186 926 901 486 481 396 304 262 197 SnaBI TAC'GTA Cuts at: 493 6396 493 Size: 5903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8605 SphI G_CATG'C Cuts at: 1132 5963 1132 Size: 4831 3774 SspI AAT'ATT Cuts at: 5 52 6529 5 Size: 47 6477 2081 Fragments arranged by size: 6477 2081 47 StuI AGG'CCT Cuts at: 1517 4769 1517 Size: 3252 5353 StyI C'CwwG_G Cuts at: 513 1065 2004 3621 6044 513 Size: 552 939 1617 2423 3074 Fragments arranged by size: 3074 2423 1617 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2837 6632 6649 6802 6987 7432 325 Size: 1255 1257 3795 17 153 185 445 1498 Fragments arranged by size: 3795 1498 1257 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3589 4900 6851 Size: 274 80 33 51 1443 1612 1311 1951 Cuts at: 6851 8563 96 Size: 1712 138 Fragments arranged by size: 1951 1712 1612 1443 1311 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8565 8597 8565 Size: 32 8573 Tth111I GACn'n_nGTC Cuts at: 1316 5803 1316 Size: 4487 4118 XbaI T'CTAG_A Cuts at: 5269 5941 5269 Size: 672 7933 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4421 4421 Size: 8605 XhoI C'TCGA_G Cuts at: 1943 2247 1943 Size: 304 8301 XmaI C'CCGG_G Cuts at: 1870 5930 1870 Size: 4060 4545 XmnI GAAnn'nnTTC Cuts at: 3501 4449 6734 3501 Size: 948 2285 5372 Fragments arranged by size: 5372 2285 948 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6413 276 Size: 53 83 186 936 4879 2468 Fragments arranged by size: 4879 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI