(Circular) (Six-base) MAPSORT of: pFN21AE1029.seq Check: 9397 from: 1 to: 8524 pFN21AE1029 8524 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2752 5050 2752 Size: 2298 6226 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6334 278 Size: 53 83 186 936 4798 2468 Fragments arranged by size: 4798 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2433 4650 5620 5867 1937 Size: 496 2217 970 247 4594 Fragments arranged by size: 4594 2217 970 496 247 Acc65I G'GTAC_C Cuts at: 5845 5845 Size: 8524 AclI AA'CG_TT Cuts at: 1563 6651 7024 1563 Size: 5088 373 3063 Fragments arranged by size: 5088 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2387 2679 3365 3960 6587 7971 8239 1632 Size: 755 292 686 595 2627 1384 268 1917 Fragments arranged by size: 2627 1917 1384 755 686 595 292 268 AfeI AGC'GCT Cuts at: 5294 5294 Size: 8524 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8327 AflIII A'CryG_T Cuts at: 1203 7423 8400 1203 Size: 6220 977 1327 Fragments arranged by size: 6220 1327 977 AgeI A'CCGG_T Cuts at: 2035 7355 2035 Size: 5320 3204 AhdI GACnn_n'nnGTC Cuts at: 2029 7253 2029 Size: 5224 3300 AleI CACnn'nnGTG Cuts at: 1166 2858 4569 1166 Size: 1692 1711 5121 Fragments arranged by size: 5121 1711 1692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3042 1184 Size: 1858 6666 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3295 3327 3295 Size: 32 8492 AlwNI CAG_nnn'CTG Cuts at: 2138 2365 2717 6267 7839 2138 Size: 227 352 3550 1572 2823 Fragments arranged by size: 3550 2823 1572 352 227 ApaI G_GGCC'C Cuts at: 2232 2232 Size: 8524 ApaLI G'TGCA_C Cuts at: 6581 7737 6581 Size: 1156 7368 ApoI r'AATT_y Cuts at: 1493 2163 2462 3448 3533 3953 5266 5323 5518 Size: 670 299 986 85 420 1313 57 195 Cuts at: 5518 5833 6059 6211 8320 1493 Size: 315 226 152 2109 1697 Fragments arranged by size: 2109 1697 1313 986 670 420 315 299 226 195 152 85 57 AseI AT'TA_AT Cuts at: 160 1032 7078 160 Size: 872 6046 1606 Fragments arranged by size: 6046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8524 AvaI C'yCGr_G Cuts at: 1870 1943 5849 1870 Size: 73 3906 4545 Fragments arranged by size: 4545 3906 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3744 3777 3744 Size: 33 8491 BamHI G'GATC_C Cuts at: 2061 5400 5854 6221 2061 Size: 3339 454 367 4364 Fragments arranged by size: 4364 3339 454 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5731 5845 7300 618 Size: 325 217 627 3944 114 1455 1842 Fragments arranged by size: 3944 1842 1455 627 325 217 114 BanII G_rGCy'C Cuts at: 1391 2216 2232 3671 5590 5843 1391 Size: 825 16 1439 1919 253 4072 Fragments arranged by size: 4072 1919 1439 825 253 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3815 4356 5471 928 Size: 971 1916 541 1115 3981 Fragments arranged by size: 3981 1916 1115 971 541 BbvCI CC'TCA_GC Cuts at: 2733 2733 Size: 8524 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6715 6749 1903 Size: 34 4778 34 3678 Fragments arranged by size: 4778 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6416 7626 6416 Size: 1210 7314 BclI T'GATC_A Cuts at: 1548 3211 5541 1548 Size: 1663 2330 4531 Fragments arranged by size: 4531 2330 1663 BfrBI ATG'CAT Cuts at: 2822 4453 2822 Size: 1631 6893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3259 3273 4550 Size: 107 122 71 631 605 1587 14 1277 Cuts at: 4550 7135 136 Size: 2585 1525 Fragments arranged by size: 2585 1587 1525 1277 631 605 122 107 71 14 BglII A'GATC_T Cuts at: 5049 8519 5049 Size: 3470 5054 BlpI GC'TnA_GC Cuts at: 3498 5166 5941 3498 Size: 1668 775 6081 Fragments arranged by size: 6081 1668 775 Bme1580I G_kGCm'C Cuts at: 1790 2232 3983 5673 5736 6585 7741 1790 Size: 442 1751 1690 63 849 1156 2573 Fragments arranged by size: 2573 1751 1690 1156 849 442 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2695 6255 7213 Size: 416 53 83 399 24 1278 3560 958 Cuts at: 7213 442 Size: 1753 Fragments arranged by size: 3560 1753 1278 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8524 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2328 3505 4511 2328 Size: 1177 1006 6341 Fragments arranged by size: 6341 1177 1006 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3011 3039 3060 3297 3655 3995 5162 5519 Size: 1772 28 21 237 358 340 1167 357 Cuts at: 5519 5741 7184 1239 Size: 222 1443 2579 Fragments arranged by size: 2579 1772 1443 1167 358 357 340 237 222 28 21 Bpu10I CC'TnA_GC Cuts at: 2733 5343 2733 Size: 2610 5914 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5113 6649 7514 7812 5113 Size: 1536 865 298 5825 Fragments arranged by size: 5825 1536 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2135 3869 5684 5753 7187 882 Size: 617 636 1734 1815 69 1434 2219 Fragments arranged by size: 2219 1815 1734 1434 636 617 69 BsaAI yAC'GTr Cuts at: 493 1204 5308 6315 493 Size: 711 4104 1007 2702 Fragments arranged by size: 4104 2702 1007 711 BsaBI GATnn'nnATC Cuts at: 8518 8518 Size: 8524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6331 6713 7348 275 Size: 53 83 186 936 4798 382 635 1451 Fragments arranged by size: 4798 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2035 5102 5403 6957 7355 7629 7776 8187 2035 Size: 3067 301 1554 398 274 147 411 2372 Fragments arranged by size: 3067 2372 1554 411 398 301 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2280 2310 3210 3240 3295 Size: 30 165 30 877 30 900 30 55 Cuts at: 3295 3325 5135 5165 1178 Size: 30 1810 30 4537 Fragments arranged by size: 4537 1810 900 877 165 55 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2721 3717 4316 5276 1186 Size: 1535 996 599 960 4434 Fragments arranged by size: 4434 1535 996 960 599 BseYI C'CCAG_C Cuts at: 2049 2183 2511 2688 2710 2721 3263 4795 4882 Size: 134 328 177 22 11 542 1532 87 Cuts at: 4882 7727 8223 2049 Size: 2845 496 2350 Fragments arranged by size: 2845 2350 1532 542 496 328 177 134 87 22 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 2316 2360 3453 4682 5078 1163 Size: 573 561 19 44 1093 1229 396 4609 Fragments arranged by size: 4609 1229 1093 573 561 396 44 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5974 6735 7763 1540 Size: 275 186 3973 761 1028 2301 Fragments arranged by size: 3973 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2216 6585 6670 7741 2216 Size: 4369 85 1071 2999 Fragments arranged by size: 4369 2999 1071 85 BsmI GAATG_Cn' Cuts at: 2984 4312 4413 6035 6128 2984 Size: 1328 101 1622 93 5380 Fragments arranged by size: 5380 1622 1328 101 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3101 4847 3101 Size: 1746 6778 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2232 3671 3983 5590 5673 5736 Size: 399 426 16 1439 312 1607 83 63 Cuts at: 5736 5843 6585 6670 7741 1391 Size: 107 742 85 1071 2174 Fragments arranged by size: 2174 1607 1439 1071 742 426 399 312 107 85 83 63 16 BspEI T'CCGG_A Cuts at: 5403 8187 5403 Size: 2784 5740 BspHI T'CATG_A Cuts at: 2454 6413 8143 2454 Size: 3959 1730 2835 Fragments arranged by size: 3959 2835 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2752 5050 5879 844 Size: 1908 2298 829 3489 Fragments arranged by size: 3489 2298 1908 829 BsrBI CCG'CTC Cuts at: 1394 1823 6411 1394 Size: 429 4588 3507 Fragments arranged by size: 4588 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3627 4071 4980 7019 7193 65 Size: 1183 2379 444 909 2039 174 1396 Fragments arranged by size: 2379 2039 1396 1183 909 444 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2035 2839 7168 7355 1700 Size: 110 225 804 4329 187 2869 Fragments arranged by size: 4329 2869 804 225 187 110 BsrGI T'GTAC_A Cuts at: 96 3586 3713 96 Size: 3490 127 4907 Fragments arranged by size: 4907 3490 127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8524 BssSI C'ACGA_G Cuts at: 3492 6584 7596 3492 Size: 3092 1012 4420 Fragments arranged by size: 4420 3092 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2101 794 Size: 1307 7217 BstBI TT'CG_AA Cuts at: 7328 7328 Size: 8524 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8524 BstXI CCAn_nnnn'nTGG Cuts at: 2599 2599 Size: 8524 BstYI r'GATC_y Cuts at: 1972 2061 3282 5049 5400 5854 6221 6606 6623 Size: 89 1221 1767 351 454 367 385 17 Cuts at: 6623 8075 8161 8173 8519 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1767 1452 1221 454 385 367 351 346 89 86 17 12 BstZ17I GTA'TAC Cuts at: 4651 4651 Size: 8524 Bsu36I CC'TnA_GG Cuts at: 2427 4235 4715 2427 Size: 1808 480 6236 Fragments arranged by size: 6236 1808 480 BtgI C'CryG_G Cuts at: 513 1065 2284 2644 4992 5469 513 Size: 552 1219 360 2348 477 3568 Fragments arranged by size: 3568 2348 1219 552 477 360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7785 Fragments arranged by size: 7785 683 56 BtsI GCAGTG_nn' Cuts at: 2139 6040 6834 6854 2139 Size: 3901 794 20 3809 Fragments arranged by size: 3901 3809 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3361 3396 529 Size: 35 2797 35 5657 Fragments arranged by size: 5657 2797 35 35 DraI TTT'AAA Cuts at: 2467 3103 3559 3747 4213 5828 2467 Size: 636 456 188 466 1615 5163 Fragments arranged by size: 5163 1615 636 466 456 188 DraIII CAC_nnn'GTG Cuts at: 2101 4396 2101 Size: 2295 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2149 4229 7531 809 Size: 507 833 2080 3302 1802 Fragments arranged by size: 3302 2080 1802 833 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2620 2787 5971 Size: 54 1425 50 275 18 790 167 3184 Cuts at: 5971 6860 8489 8 Size: 889 1629 43 Fragments arranged by size: 3184 1629 1425 889 790 275 167 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5971 1537 Size: 275 4159 4090 Fragments arranged by size: 4159 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3429 4394 4506 4518 4664 5253 6454 1353 Size: 2076 965 112 12 146 589 1201 3423 Fragments arranged by size: 3423 2076 1201 965 589 146 112 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2826 5413 7111 7485 7631 387 Size: 1064 138 1237 2587 1698 374 146 1280 Fragments arranged by size: 2587 1698 1280 1237 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2214 2214 Size: 8524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2387 2679 3011 3039 3060 3297 3365 Size: 393 755 292 332 28 21 237 68 Cuts at: 3365 3655 3960 3995 5162 5519 5741 6587 7184 Size: 290 305 35 1167 357 222 846 597 Cuts at: 7184 7971 8239 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1167 846 787 755 597 393 357 332 305 292 290 268 237 222 68 35 28 21 EcoO109I rG'GnC_Cy Cuts at: 4231 5725 4231 Size: 1494 7030 EcoRI G'AATT_C Cuts at: 2163 5266 5323 5518 5833 6211 2163 Size: 3103 57 195 315 378 4476 Fragments arranged by size: 4476 3103 378 315 195 57 FspI TGC'GCA Cuts at: 7030 7030 Size: 8524 HaeII r_GCGC'y Cuts at: 1678 5296 7671 1678 Size: 3618 2375 2531 Fragments arranged by size: 3618 2531 2375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2369 2401 3107 Size: 32 135 32 443 32 322 32 706 Cuts at: 3107 3139 3196 3210 3228 3242 4048 4080 5282 Size: 32 57 14 18 14 806 32 1202 Cuts at: 5282 5314 5321 5353 7165 7197 7239 7271 1373 Size: 32 7 32 1812 32 42 32 2626 Fragments arranged by size: 2626 1812 1202 806 706 443 322 135 57 42 32 32 32 32 32 32 32 32 32 32 18 14 14 7 HincII GTy'rAC Cuts at: 669 1657 1938 4669 5148 5414 5868 6114 669 Size: 988 281 2731 479 266 454 246 3079 Fragments arranged by size: 3079 2731 988 479 454 281 266 246 HindIII A'AGCT_T Cuts at: 748 3920 5198 748 Size: 3172 1278 4074 Fragments arranged by size: 4074 3172 1278 HpaI GTT'AAC Cuts at: 6114 6114 Size: 8524 KpnI G_GTAC'C Cuts at: 5849 5849 Size: 8524 MfeI C'AATT_G Cuts at: 4083 6123 4083 Size: 2040 6484 MluI A'CGCG_T Cuts at: 8400 8400 Size: 8524 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2039 7638 7822 1305 Size: 734 5599 184 2007 Fragments arranged by size: 5599 2007 734 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2622 10 Size: 54 1425 343 790 5912 Fragments arranged by size: 5912 1425 790 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2858 3381 3632 4569 5604 6482 6841 Size: 648 1692 523 251 937 1035 878 359 Cuts at: 6841 7000 518 Size: 159 2042 Fragments arranged by size: 2042 1692 1035 937 878 648 523 359 251 159 MspA1I CmG'CkG Cuts at: 1606 2273 2330 4037 5026 5113 5158 5233 5940 Size: 667 57 1707 989 87 45 75 707 Cuts at: 5940 6617 7765 8010 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1707 1148 989 707 677 667 245 87 75 57 45 NcoI C'CATG_G Cuts at: 513 1065 2644 4992 5469 513 Size: 552 1579 2348 477 3568 Fragments arranged by size: 3568 2348 1579 552 477 NdeI CA'TA_TG Cuts at: 387 387 Size: 8524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8524 NotI GC'GGCC_GC Cuts at: 5971 5971 Size: 8524 NsiI A_TGCA'T Cuts at: 2824 4455 2824 Size: 1631 6893 NspI r_CATG'y Cuts at: 1132 1229 5669 5882 7427 1132 Size: 97 4440 213 1545 2229 Fragments arranged by size: 4440 2229 1545 213 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8524 PciI A'CATG_T Cuts at: 7423 7423 Size: 8524 PflMI CCAn_nnn'nTGG Cuts at: 1108 5700 1108 Size: 4592 3932 PfoI T'CCnGG_A Cuts at: 2988 4890 5139 2988 Size: 1902 249 6373 Fragments arranged by size: 6373 1902 249 PmeI GTTT'AAAC Cuts at: 5828 5828 Size: 8524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3295 3327 6593 6625 8106 8138 3295 Size: 32 3266 32 1481 32 3681 Fragments arranged by size: 3681 3266 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4231 4231 Size: 8524 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8524 PsiI TTA'TAA Cuts at: 6094 6094 Size: 8524 PspOMI G'GGCC_C Cuts at: 2228 2228 Size: 8524 PstI C_TGCA'G Cuts at: 830 2412 5031 5876 830 Size: 1582 2619 845 3478 Fragments arranged by size: 3478 2619 1582 845 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8524 PvuII CAG'CTG Cuts at: 2330 4037 5026 5113 5233 2330 Size: 1707 989 87 120 5621 Fragments arranged by size: 5621 1707 989 120 87 SacI G_AGCT'C Cuts at: 2216 2216 Size: 8524 SalI G'TCGA_C Cuts at: 1936 5866 1936 Size: 3930 4594 SapI GCTCTTCn'nnn_ Cuts at: 3429 3429 Size: 8524 SbfI CC_TGCA'GG Cuts at: 5876 5876 Size: 8524 ScaI AGT'ACT Cuts at: 2171 6772 8484 2171 Size: 4601 1712 2211 Fragments arranged by size: 4601 2211 1712 SexAI A'CCwGG_T Cuts at: 2190 2397 2190 Size: 207 8317 SfcI C'TryA_G Cuts at: 826 1045 2235 2408 3297 5027 5872 7007 7688 Size: 219 1190 173 889 1730 845 1135 681 Cuts at: 7688 7879 826 Size: 191 1471 Fragments arranged by size: 1730 1471 1190 1135 889 845 681 219 191 173 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8524 SgrAI Cr'CCGG_yG Cuts at: 1700 2035 1700 Size: 335 8189 SmaI CCC'GGG Cuts at: 1872 5851 1872 Size: 3979 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5128 6628 7529 7791 820 Size: 197 926 3185 1500 901 262 1553 Fragments arranged by size: 3185 1553 1500 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6315 493 Size: 5822 2702 SpeI A'CTAG_T Cuts at: 152 3511 152 Size: 3359 5165 SphI G_CATG'C Cuts at: 1132 5882 1132 Size: 4750 3774 SspI AAT'ATT Cuts at: 5 52 6448 5 Size: 47 6396 2081 Fragments arranged by size: 6396 2081 47 StuI AGG'CCT Cuts at: 1517 5639 1517 Size: 4122 4402 StyI C'CwwG_G Cuts at: 513 1065 2644 3030 3973 4227 4992 5218 5469 Size: 552 1579 386 943 254 765 226 251 Cuts at: 5469 5658 5963 513 Size: 189 305 3074 Fragments arranged by size: 3074 1579 943 765 552 386 305 254 251 226 189 SwaI ATTT'AAAT Cuts at: 2467 2467 Size: 8524 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2197 2280 2657 5699 6551 6568 6721 Size: 1255 617 83 377 3042 852 17 153 Cuts at: 6721 6906 7351 325 Size: 185 445 1498 Fragments arranged by size: 3042 1498 1255 852 617 445 377 185 153 83 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2169 3586 3713 Size: 274 80 33 51 1443 192 1417 127 Cuts at: 3713 6770 8482 96 Size: 3057 1712 138 Fragments arranged by size: 3057 1712 1443 1417 274 192 138 127 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2280 2312 8484 8516 2280 Size: 32 6172 32 2288 Fragments arranged by size: 6172 2288 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8524 XbaI T'CTAG_A Cuts at: 5860 5860 Size: 8524 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3958 5476 3958 Size: 1518 7006 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8524 XmaI C'CCGG_G Cuts at: 1870 5849 1870 Size: 3979 4545 XmnI GAAnn'nnTTC Cuts at: 6653 6653 Size: 8524 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6332 276 Size: 53 83 186 936 4798 2468 Fragments arranged by size: 4798 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BsiWI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI