(Circular) (Six-base) MAPSORT of: pFN21AE1026.seq Check: 7344 from: 1 to: 7642 pFN21AE1026 7642 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2404 2596 2845 2404 Size: 192 249 7201 Fragments arranged by size: 7201 249 192 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3870 5452 278 Size: 53 83 186 936 2334 1582 2468 Fragments arranged by size: 2468 2334 1582 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2021 3864 4985 1937 Size: 84 1843 1121 4594 Fragments arranged by size: 4594 1843 1121 84 Acc65I G'GTAC_C Cuts at: 2597 4963 2597 Size: 2366 5276 AclI AA'CG_TT Cuts at: 1563 5769 6142 1563 Size: 4206 373 3063 Fragments arranged by size: 4206 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2343 5705 7089 7357 1632 Size: 711 3362 1384 268 1917 Fragments arranged by size: 3362 1917 1384 711 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7445 AflIII A'CryG_T Cuts at: 1203 2475 2869 2871 3081 4257 4659 6541 7518 Size: 1272 394 2 210 1176 402 1882 977 Cuts at: 7518 1203 Size: 1327 Fragments arranged by size: 1882 1327 1272 1176 977 402 394 210 2 AgeI A'CCGG_T Cuts at: 2267 6473 2267 Size: 4206 3436 AhdI GACnn_n'nnGTC Cuts at: 6371 6371 Size: 7642 AleI CACnn'nnGTG Cuts at: 1166 3242 3893 1166 Size: 2076 651 4915 Fragments arranged by size: 4915 2076 651 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3806 1184 Size: 2622 5020 AlwNI CAG_nnn'CTG Cuts at: 2437 2536 2698 2797 3418 3835 4075 4246 4327 Size: 99 162 99 621 417 240 171 81 Cuts at: 4327 4414 4930 5385 6957 2437 Size: 87 516 455 1572 3122 Fragments arranged by size: 3122 1572 621 516 455 417 240 171 162 99 99 87 81 ApaI G_GGCC'C Cuts at: 2674 3810 4865 2674 Size: 1136 1055 5451 Fragments arranged by size: 5451 1136 1055 ApaLI G'TGCA_C Cuts at: 2271 2609 5699 6855 2271 Size: 338 3090 1156 3058 Fragments arranged by size: 3090 3058 1156 338 ApoI r'AATT_y Cuts at: 1493 4951 5177 5329 7438 1493 Size: 3458 226 152 2109 1697 Fragments arranged by size: 3458 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6196 160 Size: 872 5164 1606 Fragments arranged by size: 5164 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 AvaI C'yCGr_G Cuts at: 1870 1943 2957 3809 4193 4967 1870 Size: 73 1014 852 384 774 4545 Fragments arranged by size: 4545 1014 852 774 384 73 BamHI G'GATC_C Cuts at: 4972 5339 4972 Size: 367 7275 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2113 2327 2423 2597 2857 Size: 325 217 627 326 214 96 174 260 Cuts at: 2857 3236 3494 3949 4229 4963 6418 618 Size: 379 258 455 280 734 1455 1842 Fragments arranged by size: 1842 1455 734 627 455 379 326 325 280 260 258 217 214 174 96 BanII G_rGCy'C Cuts at: 1391 2186 2238 2674 3426 3771 3810 4029 4865 Size: 795 52 436 752 345 39 219 836 Cuts at: 4865 4961 1391 Size: 96 4072 Fragments arranged by size: 4072 836 795 752 436 345 219 96 52 39 BbeI G_GCGC'C Cuts at: 3953 4233 3953 Size: 280 7362 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3457 3917 928 Size: 971 1558 460 4653 Fragments arranged by size: 4653 1558 971 460 BbvCI CC'TCA_GC Cuts at: 2154 3351 3932 2154 Size: 1197 581 5864 Fragments arranged by size: 5864 1197 581 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3079 3113 5833 5867 1903 Size: 34 1142 34 2720 34 3678 Fragments arranged by size: 3678 2720 1142 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5534 6744 5534 Size: 1210 6432 BclI T'GATC_A Cuts at: 1548 1548 Size: 7642 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2336 2388 3492 Size: 107 122 71 631 605 664 52 1104 Cuts at: 3492 4739 4892 6253 136 Size: 1247 153 1361 1525 Fragments arranged by size: 1525 1361 1247 1104 664 631 605 153 122 107 71 52 BglII A'GATC_T Cuts at: 2744 7637 2744 Size: 4893 2749 BlpI GC'TnA_GC Cuts at: 4328 5059 4328 Size: 731 6911 Bme1580I G_kGCm'C Cuts at: 1790 2275 2426 2511 2613 2674 2684 2862 3497 Size: 485 151 85 102 61 10 178 635 Cuts at: 3497 3810 4865 5703 6859 1790 Size: 313 1055 838 1156 2573 Fragments arranged by size: 2573 1156 1055 838 635 485 313 178 151 102 85 61 10 BmgBI CAC'GTC Cuts at: 1318 4099 4260 1318 Size: 2781 161 4700 Fragments arranged by size: 4700 2781 161 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3129 3936 4110 Size: 416 53 83 399 24 1712 807 174 Cuts at: 4110 5373 6331 442 Size: 1263 958 1753 Fragments arranged by size: 1753 1712 1263 958 807 416 399 174 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7642 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4390 4390 Size: 7642 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2471 2798 2834 2863 3440 3761 3940 4178 Size: 1232 327 36 29 577 321 179 238 Cuts at: 4178 4436 4478 4895 4921 6302 1239 Size: 258 42 417 26 1381 2579 Fragments arranged by size: 2579 1381 1232 577 417 327 321 258 238 179 42 36 29 26 Bpu10I CC'TnA_GC Cuts at: 2154 3351 3932 2154 Size: 1197 581 5864 Fragments arranged by size: 5864 1197 581 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2817 3608 4487 4541 5767 6632 6930 2817 Size: 791 879 54 1226 865 298 3529 Fragments arranged by size: 3529 1226 879 865 791 298 54 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2584 4697 4862 6305 882 Size: 617 1085 2113 165 1443 2219 Fragments arranged by size: 2219 2113 1443 1085 617 165 BsaAI yAC'GTr Cuts at: 493 1204 2227 2476 2827 2872 3082 3244 5433 Size: 711 1023 249 351 45 210 162 2189 Cuts at: 5433 493 Size: 2702 Fragments arranged by size: 2702 2189 1023 711 351 249 210 162 45 BsaBI GATnn'nnATC Cuts at: 7636 7636 Size: 7642 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2712 2963 3801 3867 Size: 53 83 186 936 1179 251 838 66 Cuts at: 3867 3950 4230 5449 5831 6466 275 Size: 83 280 1219 382 635 1451 Fragments arranged by size: 1451 1219 1179 936 838 635 382 280 251 186 83 83 66 53 BsaWI w'CCGG_w Cuts at: 2267 3212 4137 6075 6473 6747 6894 7305 2267 Size: 945 925 1938 398 274 147 411 2604 Fragments arranged by size: 2604 1938 945 925 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2861 2891 3422 3452 3890 Size: 30 165 30 1458 30 531 30 438 Cuts at: 3890 3920 1178 Size: 30 4900 Fragments arranged by size: 4900 1458 531 438 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4001 4211 4544 4793 4805 4808 4811 1186 Size: 2815 210 333 249 12 3 3 4017 Fragments arranged by size: 4017 2815 333 249 210 12 3 3 BseYI C'CCAG_C Cuts at: 2052 2079 2085 2107 2118 2187 2295 2427 2511 Size: 27 6 22 11 69 108 132 84 Cuts at: 2511 2601 3125 3265 4858 6845 7341 2052 Size: 90 524 140 1593 1987 496 2353 Fragments arranged by size: 2353 1987 1593 524 496 140 132 108 90 84 69 27 22 11 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2274 2438 2477 2564 2657 2810 2825 Size: 573 538 164 39 87 93 153 15 Cuts at: 2825 2861 2909 3383 3734 4382 4427 4943 1163 Size: 36 48 474 351 648 45 516 3862 Fragments arranged by size: 3862 648 573 538 516 474 351 164 153 93 87 48 45 39 36 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5092 5853 6881 1540 Size: 275 186 3091 761 1028 2301 Fragments arranged by size: 3091 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2238 2275 2613 3377 3686 3771 4011 4035 4503 Size: 37 338 764 309 85 240 24 468 Cuts at: 4503 4680 5703 5788 6859 2238 Size: 177 1023 85 1071 3021 Fragments arranged by size: 3021 1071 1023 764 468 338 309 240 177 85 85 37 24 BsmI GAATG_Cn' Cuts at: 5153 5246 5153 Size: 93 7549 BsmBI CGTCTCn'nnnn_ Cuts at: 2954 3338 3893 2954 Size: 384 555 6703 Fragments arranged by size: 6703 555 384 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 2238 2275 2426 2511 2613 2674 Size: 399 396 52 37 151 85 102 61 Cuts at: 2674 2684 2862 3377 3426 3497 3686 3771 3810 Size: 10 178 515 49 71 189 85 39 Cuts at: 3810 4011 4029 4035 4503 4680 4865 4961 5703 Size: 201 18 6 468 177 185 96 742 Cuts at: 5703 5788 6859 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 515 468 399 396 201 189 185 178 177 151 102 96 85 85 85 71 61 52 49 39 37 18 10 6 BspEI T'CCGG_A Cuts at: 4137 7305 4137 Size: 3168 4474 BspHI T'CATG_A Cuts at: 5531 7261 5531 Size: 1730 5912 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2404 2596 2692 2845 3888 4997 844 Size: 1560 192 96 153 1043 1109 3489 Fragments arranged by size: 3489 1560 1109 1043 192 153 96 BsrBI CCG'CTC Cuts at: 1394 1823 3459 5529 1394 Size: 429 1636 2070 3507 Fragments arranged by size: 3507 2070 1636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6137 6311 65 Size: 1183 4889 174 1396 Fragments arranged by size: 4889 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2267 2387 2632 4523 4882 6286 6473 Size: 110 457 120 245 1891 359 1404 187 Cuts at: 6473 1700 Size: 2869 Fragments arranged by size: 2869 1891 1404 457 359 245 187 120 110 BsrGI T'GTAC_A Cuts at: 96 2970 3964 96 Size: 2874 994 3774 Fragments arranged by size: 3774 2874 994 BssHII G'CGCG_C Cuts at: 1925 4286 4288 4907 1925 Size: 2361 2 619 4660 Fragments arranged by size: 4660 2361 619 2 BssSI C'ACGA_G Cuts at: 4375 5702 6714 4375 Size: 1327 1012 5303 Fragments arranged by size: 5303 1327 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2437 4246 4930 794 Size: 1643 1809 684 3506 Fragments arranged by size: 3506 1809 1643 684 BstBI TT'CG_AA Cuts at: 6446 6446 Size: 7642 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7642 BstXI CCAn_nnnn'nTGG Cuts at: 3524 3661 4156 3524 Size: 137 495 7010 Fragments arranged by size: 7010 495 137 BstYI r'GATC_y Cuts at: 1972 2744 4972 5339 5724 5741 7193 7279 7291 Size: 772 2228 367 385 17 1452 86 12 Cuts at: 7291 7637 1972 Size: 346 1977 Fragments arranged by size: 2228 1977 1452 772 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2022 2022 Size: 7642 Bsu36I CC'TnA_GG Cuts at: 3795 3795 Size: 7642 BtgI C'CryG_G Cuts at: 513 1065 2004 2593 2676 2707 3315 3368 4179 Size: 552 939 589 83 31 608 53 811 Cuts at: 4179 4221 4578 513 Size: 42 357 3577 Fragments arranged by size: 3577 939 811 608 589 552 357 83 53 42 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2922 4696 487 Size: 683 56 1696 1774 3433 Fragments arranged by size: 3433 1774 1696 683 56 BtsI GCAGTG_nn' Cuts at: 3067 3163 3208 3277 3400 4244 4469 5158 5952 Size: 96 45 69 123 844 225 689 794 Cuts at: 5952 5972 3067 Size: 20 4737 Fragments arranged by size: 4737 844 794 689 225 123 96 69 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7642 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7607 DraI TTT'AAA Cuts at: 4946 4946 Size: 7642 DraIII CAC_nnn'GTG Cuts at: 2210 2210 Size: 7642 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6649 809 Size: 507 5333 1802 Fragments arranged by size: 5333 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2212 2572 2922 Size: 54 1425 50 275 18 382 360 350 Cuts at: 2922 3015 3054 3585 4218 4740 4884 5089 5978 Size: 93 39 531 633 522 144 205 889 Cuts at: 5978 7607 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 633 531 522 382 360 350 275 205 144 93 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 5089 1537 Size: 275 3277 4090 Fragments arranged by size: 4090 3277 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2526 2656 4553 4782 4922 5572 1353 Size: 1173 130 1897 229 140 650 3423 Fragments arranged by size: 3423 1897 1173 650 229 140 130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3100 6229 6603 6749 387 Size: 1064 138 1511 3129 374 146 1280 Fragments arranged by size: 3129 1511 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2236 3769 2236 Size: 1533 6109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2343 2471 2798 2834 2863 3440 3761 Size: 393 711 128 327 36 29 577 321 Cuts at: 3761 3940 4178 4436 4478 4895 4921 5705 6302 Size: 179 238 258 42 417 26 784 597 Cuts at: 6302 7089 7357 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 784 711 597 577 417 393 327 321 268 258 238 179 128 42 36 29 26 EcoNI CCTnn'n_nnAGG Cuts at: 3919 4277 3919 Size: 358 7284 EcoO109I rG'GnC_Cy Cuts at: 2065 2670 2671 2809 3832 4072 4591 2065 Size: 605 1 138 1023 240 519 5116 Fragments arranged by size: 5116 1023 605 519 240 138 1 EcoRI G'AATT_C Cuts at: 4951 5329 4951 Size: 378 7264 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4582 4582 Size: 7642 FspI TGC'GCA Cuts at: 3533 4604 6148 3533 Size: 1071 1544 5027 Fragments arranged by size: 5027 1544 1071 HaeII r_GCGC'y Cuts at: 1678 2774 3708 3953 4233 4255 4524 4611 4931 Size: 1096 934 245 280 22 269 87 320 Cuts at: 4931 6789 1678 Size: 1858 2531 Fragments arranged by size: 2531 1858 1096 934 320 280 269 245 87 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2336 2368 2736 2768 6283 Size: 32 135 32 764 32 368 32 3515 Cuts at: 6283 6315 6357 6389 1373 Size: 32 42 32 2626 Fragments arranged by size: 3515 2626 764 368 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3430 3865 4186 4986 5232 669 Size: 988 281 1492 435 321 800 246 3079 Fragments arranged by size: 3079 1492 988 800 435 321 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7642 HpaI GTT'AAC Cuts at: 5232 5232 Size: 7642 KasI G'GCGC_C Cuts at: 3949 4229 3949 Size: 280 7362 KpnI G_GTAC'C Cuts at: 2601 4967 2601 Size: 2366 5276 MfeI C'AATT_G Cuts at: 5241 5241 Size: 7642 MluI A'CGCG_T Cuts at: 7518 7518 Size: 7642 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2334 3675 6756 6940 1305 Size: 1029 1341 3081 184 2007 Fragments arranged by size: 3081 2007 1341 1029 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2214 2574 3056 3587 4742 Size: 54 1425 343 382 360 482 531 1155 Cuts at: 4742 10 Size: 2910 Fragments arranged by size: 2910 1425 1155 531 482 382 360 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2504 3074 3080 3242 3522 3587 3893 Size: 648 1338 570 6 162 280 65 306 Cuts at: 3893 4004 5600 5959 6118 518 Size: 111 1596 359 159 2042 Fragments arranged by size: 2042 1596 1338 648 570 359 306 280 162 159 111 65 6 MspA1I CmG'CkG Cuts at: 1606 2111 2141 2434 2491 2698 2797 2968 3144 Size: 505 30 293 57 207 99 171 176 Cuts at: 3144 3228 3958 4223 4389 4411 5058 5735 6883 Size: 84 730 265 166 22 647 677 1148 Cuts at: 6883 7128 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 730 677 647 505 293 265 245 207 176 171 166 99 84 57 30 22 NaeI GCC'GGC Cuts at: 4525 4884 4525 Size: 359 7283 NarI GG'CG_CC Cuts at: 3950 4230 3950 Size: 280 7362 NcoI C'CATG_G Cuts at: 513 1065 2004 2593 4179 4578 513 Size: 552 939 589 1586 399 3577 Fragments arranged by size: 3577 1586 939 589 552 399 NdeI CA'TA_TG Cuts at: 387 387 Size: 7642 NgoMIV G'CCGG_C Cuts at: 4523 4882 4523 Size: 359 7283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7642 NotI GC'GGCC_GC Cuts at: 5089 5089 Size: 7642 NspI r_CATG'y Cuts at: 1132 1229 3265 4663 5000 6545 1132 Size: 97 2036 1398 337 1545 2229 Fragments arranged by size: 2229 2036 1545 1398 337 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7642 PciI A'CATG_T Cuts at: 4659 6541 4659 Size: 1882 5760 PflMI CCAn_nnn'nTGG Cuts at: 1108 2210 3052 3640 4075 4180 1108 Size: 1102 842 588 435 105 4570 Fragments arranged by size: 4570 1102 842 588 435 105 PfoI T'CCnGG_A Cuts at: 3330 4587 3330 Size: 1257 6385 PmeI GTTT'AAAC Cuts at: 4946 4946 Size: 7642 PmlI CAC'GTG Cuts at: 2476 2827 2872 2476 Size: 351 45 7246 Fragments arranged by size: 7246 351 45 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5711 5743 7224 7256 5711 Size: 32 1481 32 6097 Fragments arranged by size: 6097 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3832 4591 3832 Size: 759 6883 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7642 PsiI TTA'TAA Cuts at: 5212 5212 Size: 7642 PspOMI G'GGCC_C Cuts at: 2670 3806 4861 2670 Size: 1136 1055 5451 Fragments arranged by size: 5451 1136 1055 PstI C_TGCA'G Cuts at: 830 2181 2703 3885 4302 4443 4994 830 Size: 1351 522 1182 417 141 551 3478 Fragments arranged by size: 3478 1351 1182 551 522 417 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7642 PvuII CAG'CTG Cuts at: 2491 2698 2491 Size: 207 7435 SacI G_AGCT'C Cuts at: 2238 3771 2238 Size: 1533 6109 SacII CC_GC'GG Cuts at: 4224 4224 Size: 7642 SalI G'TCGA_C Cuts at: 1936 3863 4984 1936 Size: 1927 1121 4594 Fragments arranged by size: 4594 1927 1121 SapI GCTCTTCn'nnn_ Cuts at: 2526 2656 4553 2526 Size: 130 1897 5615 Fragments arranged by size: 5615 1897 130 SbfI CC_TGCA'GG Cuts at: 4994 4994 Size: 7642 ScaI AGT'ACT Cuts at: 5890 7602 5890 Size: 1712 5930 SfcI C'TryA_G Cuts at: 826 1045 2030 2177 2699 3881 4298 4439 4570 Size: 219 985 147 522 1182 417 141 131 Cuts at: 4570 4990 6125 6806 6997 826 Size: 420 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 985 681 522 420 417 219 191 147 141 131 SfoI GGC'GCC Cuts at: 3951 4231 3951 Size: 280 7362 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 SgrAI Cr'CCGG_yG Cuts at: 1700 2632 1700 Size: 932 6710 SmaI CCC'GGG Cuts at: 1872 3811 4969 1872 Size: 1939 1158 4545 Fragments arranged by size: 4545 1939 1158 SmlI C'TyrA_G Cuts at: 820 1017 1943 2832 3623 4502 4556 5746 6647 Size: 197 926 889 791 879 54 1190 901 Cuts at: 6647 6909 820 Size: 262 1553 Fragments arranged by size: 1553 1190 926 901 889 879 791 262 197 54 SnaBI TAC'GTA Cuts at: 493 5433 493 Size: 4940 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7642 SphI G_CATG'C Cuts at: 1132 5000 1132 Size: 3868 3774 SspI AAT'ATT Cuts at: 5 52 5566 5 Size: 47 5514 2081 Fragments arranged by size: 5514 2081 47 StuI AGG'CCT Cuts at: 1517 4373 1517 Size: 2856 4786 StyI C'CwwG_G Cuts at: 513 1065 2004 2593 3250 4179 4533 4578 4695 Size: 552 939 589 657 929 354 45 117 Cuts at: 4695 5081 513 Size: 386 3074 Fragments arranged by size: 3074 939 929 657 589 552 386 354 117 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2149 2290 2441 2750 3035 4187 4369 Size: 1255 569 141 151 309 285 1152 182 Cuts at: 4369 5669 5686 5839 6024 6469 325 Size: 1300 17 153 185 445 1498 Fragments arranged by size: 1498 1300 1255 1152 569 445 309 285 185 182 153 151 141 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 2874 2970 Size: 274 80 33 51 1443 324 573 96 Cuts at: 2970 3964 4656 4662 5888 7600 96 Size: 994 692 6 1226 1712 138 Fragments arranged by size: 1712 1443 1226 994 692 573 324 274 138 96 80 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2861 2893 3890 3922 7602 7634 2861 Size: 32 997 32 3680 32 2869 Fragments arranged by size: 3680 2869 997 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2350 1316 Size: 1034 6608 XbaI T'CTAG_A Cuts at: 4978 4978 Size: 7642 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2533 3637 4000 4717 2533 Size: 1104 363 717 5458 Fragments arranged by size: 5458 1104 717 363 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7642 XmaI C'CCGG_G Cuts at: 1870 3809 4967 1870 Size: 1939 1158 4545 Fragments arranged by size: 4545 1939 1158 XmnI GAAnn'nnTTC Cuts at: 3914 5771 3914 Size: 1857 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3868 5450 276 Size: 53 83 186 936 2334 1582 2468 Fragments arranged by size: 2468 2334 1582 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI