(Circular) (Six-base) MAPSORT of: pFN21AB5623.seq Check: 4351 from: 1 to: 7642 pFN21AB5623 7642 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5452 278 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4985 1937 Size: 3048 4594 Acc65I G'GTAC_C Cuts at: 3981 4963 3981 Size: 982 6660 AclI AA'CG_TT Cuts at: 1563 2089 5769 6142 1563 Size: 526 3680 373 3063 Fragments arranged by size: 3680 3063 526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3784 3864 5705 7089 7357 1632 Size: 2152 80 1841 1384 268 1917 Fragments arranged by size: 2152 1917 1841 1384 268 80 AflII C'TTAA_G Cuts at: 820 1017 2950 3175 820 Size: 197 1933 225 5287 Fragments arranged by size: 5287 1933 225 197 AflIII A'CryG_T Cuts at: 1203 2135 2584 6541 7518 1203 Size: 932 449 3957 977 1327 Fragments arranged by size: 3957 1327 977 932 449 AgeI A'CCGG_T Cuts at: 6473 6473 Size: 7642 AhdI GACnn_n'nnGTC Cuts at: 6371 6371 Size: 7642 AleI CACnn'nnGTG Cuts at: 1166 2267 1166 Size: 1101 6541 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7642 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2435 2467 2435 Size: 32 7610 AlwNI CAG_nnn'CTG Cuts at: 2287 4407 4601 5385 6957 2287 Size: 2120 194 784 1572 2972 Fragments arranged by size: 2972 2120 1572 784 194 ApaLI G'TGCA_C Cuts at: 3068 5699 6855 3068 Size: 2631 1156 3855 Fragments arranged by size: 3855 2631 1156 ApoI r'AATT_y Cuts at: 1493 2383 3804 4364 4951 5177 5329 7438 1493 Size: 890 1421 560 587 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 890 587 560 226 152 AseI AT'TA_AT Cuts at: 160 1032 4358 6196 160 Size: 872 3326 1838 1606 Fragments arranged by size: 3326 1838 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 AvaI C'yCGr_G Cuts at: 1870 1943 4967 1870 Size: 73 3024 4545 Fragments arranged by size: 4545 3024 73 AvrII C'CTAG_G Cuts at: 4465 4465 Size: 7642 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2091 2124 3965 3998 2091 Size: 33 1841 33 5735 Fragments arranged by size: 5735 1841 33 33 BamHI G'GATC_C Cuts at: 2776 4972 5339 2776 Size: 2196 367 5079 Fragments arranged by size: 5079 2196 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2486 3981 4819 4963 6418 Size: 325 217 627 699 1495 838 144 1455 Cuts at: 6418 618 Size: 1842 Fragments arranged by size: 1842 1495 1455 838 699 627 325 217 144 BanII G_rGCy'C Cuts at: 1391 2313 2724 3453 4961 1391 Size: 922 411 729 1508 4072 Fragments arranged by size: 4072 1508 922 729 411 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6671 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5833 5867 1903 Size: 34 3896 34 3678 Fragments arranged by size: 3896 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2545 3851 3898 4421 4642 5534 6744 2545 Size: 1306 47 523 221 892 1210 3443 Fragments arranged by size: 3443 1306 1210 892 523 221 47 BclI T'GATC_A Cuts at: 1548 1548 Size: 7642 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6253 136 Size: 107 122 71 631 605 4581 1525 Fragments arranged by size: 4581 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7637 7637 Size: 7642 BlpI GC'TnA_GC Cuts at: 5059 5059 Size: 7642 Bme1580I G_kGCm'C Cuts at: 1790 3072 5703 6859 1790 Size: 1282 2631 1156 2573 Fragments arranged by size: 2631 2573 1282 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7642 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4170 4756 4805 Size: 416 53 83 399 24 2753 586 49 Cuts at: 4805 5373 6331 442 Size: 568 958 1753 Fragments arranged by size: 2753 1753 958 586 568 416 399 83 53 49 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7642 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2410 2419 3334 3678 4755 6302 1239 Size: 1171 9 915 344 1077 1547 2579 Fragments arranged by size: 2579 1547 1171 1077 915 344 9 Bpu10I CC'TnA_GC Cuts at: 2376 2376 Size: 7642 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2077 2735 2886 5767 6632 6930 2077 Size: 658 151 2881 865 298 2789 Fragments arranged by size: 2881 2789 865 658 298 151 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4301 6305 882 Size: 617 2802 2004 2219 Fragments arranged by size: 2802 2219 2004 617 BsaAI yAC'GTr Cuts at: 493 1204 3895 5433 493 Size: 711 2691 1538 2702 Fragments arranged by size: 2702 2691 1538 711 BsaBI GATnn'nnATC Cuts at: 2213 4232 7636 2213 Size: 2019 3404 2219 Fragments arranged by size: 3404 2219 2019 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5449 5831 6466 275 Size: 53 83 186 936 3916 382 635 1451 Fragments arranged by size: 3916 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4204 6075 6473 6747 6894 7305 4204 Size: 1871 398 274 147 411 4541 Fragments arranged by size: 4541 1871 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4697 4727 1178 Size: 30 165 30 3294 30 4093 Fragments arranged by size: 4093 3294 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2732 4207 4894 1186 Size: 1546 1475 687 3934 Fragments arranged by size: 3934 1546 1475 687 BseYI C'CCAG_C Cuts at: 2298 4654 4729 6845 7341 2298 Size: 2356 75 2116 496 2599 Fragments arranged by size: 2599 2356 2116 496 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7069 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4887 5092 5853 6881 1540 Size: 275 186 2886 205 761 1028 2301 Fragments arranged by size: 2886 2301 1028 761 275 205 186 BsiHKAI G_wGCw'C Cuts at: 2313 3072 3453 3711 5703 5788 6859 2313 Size: 759 381 258 1992 85 1071 3096 Fragments arranged by size: 3096 1992 1071 759 381 258 85 BsmI GAATG_Cn' Cuts at: 2594 5153 5246 2594 Size: 2559 93 4990 Fragments arranged by size: 4990 2559 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2313 2724 3072 3453 3711 4961 5703 Size: 399 523 411 348 381 258 1250 742 Cuts at: 5703 5788 6859 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1250 1071 742 523 411 399 381 348 258 85 BspEI T'CCGG_A Cuts at: 4204 7305 4204 Size: 3101 4541 BspHI T'CATG_A Cuts at: 3054 5531 7261 3054 Size: 2477 1730 3435 Fragments arranged by size: 3435 2477 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3199 4997 844 Size: 2355 1798 3489 Fragments arranged by size: 3489 2355 1798 BsrBI CCG'CTC Cuts at: 1394 1823 5529 1394 Size: 429 3706 3507 Fragments arranged by size: 3706 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2045 6137 6311 65 Size: 1183 797 4092 174 1396 Fragments arranged by size: 4092 1396 1183 797 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6286 6473 1700 Size: 110 4476 187 2869 Fragments arranged by size: 4476 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2422 2867 96 Size: 2326 445 4871 Fragments arranged by size: 4871 2326 445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7642 BssSI C'ACGA_G Cuts at: 5702 6714 5702 Size: 1012 6630 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7642 BstBI TT'CG_AA Cuts at: 6446 6446 Size: 7642 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7642 BstXI CCAn_nnnn'nTGG Cuts at: 2388 2388 Size: 7642 BstYI r'GATC_y Cuts at: 1972 2208 2776 3396 4921 4972 5339 5724 5741 Size: 236 568 620 1525 51 367 385 17 Cuts at: 5741 7193 7279 7291 7637 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1525 1452 620 568 385 367 346 236 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3115 4245 513 Size: 552 939 1111 1130 3910 Fragments arranged by size: 3910 1130 1111 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6903 Fragments arranged by size: 6903 683 56 BtsI GCAGTG_nn' Cuts at: 2790 4019 5158 5952 5972 2790 Size: 1229 1139 794 20 4460 Fragments arranged by size: 4460 1229 1139 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7642 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2317 2352 529 Size: 35 1753 35 5819 Fragments arranged by size: 5819 1753 35 35 DraI TTT'AAA Cuts at: 4946 4946 Size: 7642 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6649 809 Size: 507 5333 1802 Fragments arranged by size: 5333 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4618 5089 5978 Size: 54 1425 50 275 18 2788 471 889 Cuts at: 5978 7607 8 Size: 1629 43 Fragments arranged by size: 2788 1629 1425 889 471 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5089 1537 Size: 275 3277 4090 Fragments arranged by size: 4090 3277 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2951 5572 1353 Size: 1598 2621 3423 Fragments arranged by size: 3423 2621 1598 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6229 6603 6749 387 Size: 1064 138 4640 374 146 1280 Fragments arranged by size: 4640 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2311 3451 2311 Size: 1140 6502 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2410 2419 3334 3678 3784 3864 4755 Size: 393 778 9 915 344 106 80 891 Cuts at: 4755 5705 6302 7089 7357 1239 Size: 950 597 787 268 1524 Fragments arranged by size: 1524 950 915 891 787 778 597 393 344 268 106 80 9 EcoO109I rG'GnC_Cy Cuts at: 3301 3737 4172 3301 Size: 436 435 6771 Fragments arranged by size: 6771 436 435 EcoRI G'AATT_C Cuts at: 4951 5329 4951 Size: 378 7264 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2847 3849 2847 Size: 1002 6640 FspI TGC'GCA Cuts at: 6148 6148 Size: 7642 HaeII r_GCGC'y Cuts at: 1678 6789 1678 Size: 5111 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3422 3454 4199 4231 4300 Size: 32 135 32 1850 32 745 32 69 Cuts at: 4300 4332 4595 4627 6283 6315 6357 6389 1373 Size: 32 263 32 1656 32 42 32 2626 Fragments arranged by size: 2626 1850 1656 745 263 135 69 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3022 4500 4986 5232 669 Size: 988 281 1084 1478 486 246 3079 Fragments arranged by size: 3079 1478 1084 988 486 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7642 HpaI GTT'AAC Cuts at: 5232 5232 Size: 7642 KpnI G_GTAC'C Cuts at: 3985 4967 3985 Size: 982 6660 MfeI C'AATT_G Cuts at: 5241 5241 Size: 7642 MluI A'CGCG_T Cuts at: 2135 7518 2135 Size: 5383 2259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3917 4553 6756 6940 1305 Size: 2612 636 2203 184 2007 Fragments arranged by size: 2612 2203 2007 636 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4620 10 Size: 54 1425 343 2788 3032 Fragments arranged by size: 3032 2788 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2267 3097 3155 3387 4274 5600 Size: 648 1080 21 830 58 232 887 1326 Cuts at: 5600 5959 6118 518 Size: 359 159 2042 Fragments arranged by size: 2042 1326 1080 887 830 648 359 232 159 58 21 MspA1I CmG'CkG Cuts at: 1606 4733 5058 5735 6883 7128 1606 Size: 3127 325 677 1148 245 2120 Fragments arranged by size: 3127 2120 1148 677 325 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3115 4245 513 Size: 552 939 1111 1130 3910 Fragments arranged by size: 3910 1130 1111 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7642 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7642 NotI GC'GGCC_GC Cuts at: 5089 5089 Size: 7642 NspI r_CATG'y Cuts at: 1132 1229 2588 3386 5000 6545 1132 Size: 97 1359 798 1614 1545 2229 Fragments arranged by size: 2229 1614 1545 1359 798 97 PasI CC'CwG_GG Cuts at: 1348 4717 1348 Size: 3369 4273 PciI A'CATG_T Cuts at: 2584 6541 2584 Size: 3957 3685 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7642 PmeI GTTT'AAAC Cuts at: 4946 4946 Size: 7642 PmlI CAC'GTG Cuts at: 3895 3895 Size: 7642 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5711 5743 7224 7256 5711 Size: 32 1481 32 6097 Fragments arranged by size: 6097 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3737 3737 Size: 7642 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7642 PsiI TTA'TAA Cuts at: 5212 5212 Size: 7642 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3152 3184 3152 Size: 32 7610 PstI C_TGCA'G Cuts at: 830 3577 4994 830 Size: 2747 1417 3478 Fragments arranged by size: 3478 2747 1417 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7642 PvuII CAG'CTG Cuts at: 4733 4733 Size: 7642 SacI G_AGCT'C Cuts at: 2313 3453 2313 Size: 1140 6502 SalI G'TCGA_C Cuts at: 1936 4984 1936 Size: 3048 4594 SbfI CC_TGCA'GG Cuts at: 4994 4994 Size: 7642 ScaI AGT'ACT Cuts at: 2712 3170 5890 7602 2712 Size: 458 2720 1712 2752 Fragments arranged by size: 2752 2720 1712 458 SexAI A'CCwGG_T Cuts at: 2280 3964 2280 Size: 1684 5958 SfcI C'TryA_G Cuts at: 826 1045 3573 4990 6125 6806 6997 826 Size: 219 2528 1417 1135 681 191 1471 Fragments arranged by size: 2528 1471 1417 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7642 SmaI CCC'GGG Cuts at: 1872 4969 1872 Size: 3097 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2056 2714 2901 2950 3175 5746 Size: 197 926 113 658 187 49 225 2571 Cuts at: 5746 6647 6909 820 Size: 901 262 1553 Fragments arranged by size: 2571 1553 926 901 658 262 225 197 187 113 49 SnaBI TAC'GTA Cuts at: 493 5433 493 Size: 4940 2702 SpeI A'CTAG_T Cuts at: 152 4157 152 Size: 4005 3637 SphI G_CATG'C Cuts at: 1132 3386 5000 1132 Size: 2254 1614 3774 Fragments arranged by size: 3774 2254 1614 SspI AAT'ATT Cuts at: 5 52 5566 5 Size: 47 5514 2081 Fragments arranged by size: 5514 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7642 StyI C'CwwG_G Cuts at: 513 1065 2004 2724 2796 3115 4002 4245 4465 Size: 552 939 720 72 319 887 243 220 Cuts at: 4465 5081 513 Size: 616 3074 Fragments arranged by size: 3074 939 887 720 616 552 319 243 220 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2236 3530 4035 4789 4901 5669 5686 Size: 1255 656 1294 505 754 112 768 17 Cuts at: 5686 5839 6024 6469 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1294 1255 768 754 656 505 445 185 153 112 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2422 2710 2768 Size: 274 80 33 51 1443 445 288 58 Cuts at: 2768 2867 3168 3201 4681 5888 7600 96 Size: 99 301 33 1480 1207 1712 138 Fragments arranged by size: 1712 1480 1443 1207 445 301 288 274 138 99 80 58 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2947 2979 3235 3267 7602 7634 2947 Size: 32 256 32 4335 32 2955 Fragments arranged by size: 4335 2955 256 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7642 XbaI T'CTAG_A Cuts at: 4978 4978 Size: 7642 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3202 3535 3202 Size: 333 7309 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7642 XmaI C'CCGG_G Cuts at: 1870 4967 1870 Size: 3097 4545 XmnI GAAnn'nnTTC Cuts at: 3086 5771 3086 Size: 2685 4957 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5450 276 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI