Restriction map for
pFN21AB5446
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII AccI AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbsI BciVI BclI
BfrI BglI BlnI BpuAI BsaAI
BsaHI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KspI MfeI MluI MluNI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1048 GGTACC G^GTACC
AccIII 1373 TCCGGA T^CCGGA
AcsI 1115 AAATTC [GA]^AATT[CT]
ApoI 1115 AAATTC [GA]^AATT[CT]
Asp700I 1681 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1048 GGTACC G^GTACC
AvaII 1212 GGACC G^G[AT]CC
BamHI 1838 GGATCC G^GATCC
BglII 1359 AGATCT A^GATCT
BlpI 133 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 133 GCTCAGC GC^T[ACGT]AGC
BsaBI 897 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 388 GAGACC GGTCTC(1/5)
BsaWI 1373 TCCGGA [AT]^CCGG[AT]
BseAI 1373 TCCGGA T^CCGGA
BseRI 385 GAGGAG GAGGAG(10/8)
BspEI 1373 TCCGGA T^CCGGA
BspHI 1325 TCATGA T^CATGA
BspMI 1045 GCAGGT ACCTGC(4/8)
BsrDI 1065 CATTGC GCAATG(2/0)
BsrFI 192 ACCGGC [GA]^CCGG[CT]
BstEII 1941 GGTCACC G^GT[ACGT]ACC
BstPI 1941 GGTCACC G^GT[ACGT]ACC
CelII 133 GCTCAGC GC^T[ACGT]AGC
CfoI 76 GCGC GCG^C
Cfr10I 192 ACCGGC [GA]^CCGG[CT]
DraIII 1812 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 286 GAAGAG CTCTTC(1/4)
EcoNI 1764 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1941 GGTCACC G^GT[ACGT]ACC
HaeII 75 GGCGCT [GA]GCGC^[CT]
HhaI 76 GCGC GCG^C
HinP1I 76 GCGC G^CGC
HindIII 739 AAGCTT A^AGCTT
KpnI 1048 GGTACC GGTAC^C
Ksp632I 286 GAAGAG CTCTTC(1/4)
MaeII 962 ACGT A^CGT
MamI 897 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1373 TCCGGA T^CCGGA
MseI 305 TTAA T^TAA
NspI 503 GCATGC [GA]CATG^[CT]
PaeR7I 1746 CTCGAG C^TCGAG
PflMI 1302 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1325 TCATGA T^CATGA
SapI 286 GAAGAGC GCTCTTC(1/4)
SexAI 1813 ACCTGGT A^CC[AT]GGT
SphI 503 GCATGC GCATG^C
StuI 1916 AGGCCT AGG^CCT
Tru9I 305 TTAA T^TAA
Tsp509I 1116 AATT ^AATT
Van91I 1302 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1746 CTCGAG C^TCGAG
XmnI 1681 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 21 CGCG CG^CG
65 CGCG
ApaI 536 GGGCCC GGGCC^C
880 GGGCCC
AvaI 49 CTCGGG C^[CT]CG[GA]G
1746 CTCGAG
BbvCI 42 GCTGAGG CCTCAGC(-5/-2)
1524 GCTGAGG
BcgI 1719 CGACCTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3 GCAGGCGGCTCG
BfaI 763 CTAG C^TAG
1504 CTAG
BsmFI 1387 GGGAC GGGAC(10/14)
327 GTCCC
BsmI 1294 GAATGC GAATGC(1/-1)
1488 GAATGC
BsoBI 49 CTCGGG C^[CT]CG[GA]G
1746 CTCGAG
BsrBI 131 CCGCTC CCGCTC(-3/-3)
1927 GAGCGG
BstUI 21 CGCG CG^CG
65 CGCG
EcoT22I 505 ATGCAT ATGCA^T
1296 ATGCAT
HgaI 172 GACGC GACGC(5/10)
367 GACGC
MaeI 763 CTAG C^TAG
1504 CTAG
MslI 1632 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1731 CATGGTGGTG
MvnI 21 CGCG CG^CG
65 CGCG
NsiI 505 ATGCAT ATGCA^T
1296 ATGCAT
PvuII 136 CAGCTG CAG^CTG
1522 CAGCTG
SacI 1447 GAGCTC GAGCT^C
1885 GAGCTC
XcmI 1184 CCATGGCCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1913 CCAAGGCCTTGGTGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII