(Circular) (Six-base) MAPSORT of: pFN21AB5446.seq Check: 4247 from: 1 to: 6670 pFN21AB5446 6670 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4480 278 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4013 1937 Size: 2076 4594 Acc65I G'GTAC_C Cuts at: 3053 3991 3053 Size: 938 5732 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4733 6117 6385 1632 Size: 3101 1384 268 1917 Fragments arranged by size: 3101 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6473 AflIII A'CryG_T Cuts at: 1203 5569 6546 1203 Size: 4366 977 1327 Fragments arranged by size: 4366 1327 977 AgeI A'CCGG_T Cuts at: 5501 5501 Size: 6670 AhdI GACnn_n'nnGTC Cuts at: 5399 5399 Size: 6670 AleI CACnn'nnGTG Cuts at: 1166 3641 1166 Size: 2475 4195 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6670 AlwNI CAG_nnn'CTG Cuts at: 2047 2712 3694 3706 4413 5985 2047 Size: 665 982 12 707 1572 2732 Fragments arranged by size: 2732 1572 982 707 665 12 ApaI G_GGCC'C Cuts at: 2545 2889 2545 Size: 344 6326 ApaLI G'TGCA_C Cuts at: 4727 5883 4727 Size: 1156 5514 ApoI r'AATT_y Cuts at: 1493 3120 3979 4205 4357 6466 1493 Size: 1627 859 226 152 2109 1697 Fragments arranged by size: 2109 1697 1627 859 226 152 AseI AT'TA_AT Cuts at: 160 1032 5224 160 Size: 872 4192 1606 Fragments arranged by size: 4192 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 2054 3751 3995 1870 Size: 73 111 1697 244 4545 Fragments arranged by size: 4545 1697 244 111 73 BamHI G'GATC_C Cuts at: 3843 4000 4367 3843 Size: 157 367 6146 Fragments arranged by size: 6146 367 157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 2953 3053 3991 5446 Size: 325 217 627 345 821 100 938 1455 Cuts at: 5446 618 Size: 1842 Fragments arranged by size: 1842 1455 938 821 627 345 325 217 100 BanII G_rGCy'C Cuts at: 1391 2545 2661 2889 3456 3622 3894 3989 1391 Size: 1154 116 228 567 166 272 95 4072 Fragments arranged by size: 4072 1154 567 272 228 166 116 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5699 BbvCI CC'TCA_GC Cuts at: 2048 3530 2048 Size: 1482 5188 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 1997 2021 2031 3713 3747 4861 Size: 34 50 10 24 10 1682 34 1114 Cuts at: 4861 4895 1903 Size: 34 3678 Fragments arranged by size: 3678 1682 1114 50 34 34 34 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 4562 5772 4562 Size: 1210 5460 BclI T'GATC_A Cuts at: 1548 1548 Size: 6670 BfrBI ATG'CAT Cuts at: 2512 3303 2512 Size: 791 5879 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5281 136 Size: 107 122 71 631 605 337 3272 1525 Fragments arranged by size: 3272 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3364 6665 3364 Size: 3301 3369 BlpI GC'TnA_GC Cuts at: 2139 4087 2139 Size: 1948 4722 Bme1580I G_kGCm'C Cuts at: 1790 2445 2545 2706 2889 4731 5887 1790 Size: 655 100 161 183 1842 1156 2573 Fragments arranged by size: 2573 1842 1156 655 183 161 100 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6670 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2439 2473 3100 Size: 416 53 83 399 24 1022 34 627 Cuts at: 3100 4401 5359 442 Size: 1301 958 1753 Fragments arranged by size: 1753 1301 1022 958 627 416 399 83 53 34 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2399 3363 3644 5330 1239 Size: 1160 964 281 1686 2579 Fragments arranged by size: 2579 1686 1160 964 281 Bpu10I CC'TnA_GC Cuts at: 2048 3075 3530 2048 Size: 1027 455 5188 Fragments arranged by size: 5188 1027 455 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2396 2647 3745 4795 5660 5958 2396 Size: 251 1098 1050 865 298 3108 Fragments arranged by size: 3108 1098 1050 865 298 251 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2387 5333 882 Size: 617 888 2946 2219 Fragments arranged by size: 2946 2219 888 617 BsaAI yAC'GTr Cuts at: 493 1204 4461 493 Size: 711 3257 2702 Fragments arranged by size: 3257 2702 711 BsaBI GATnn'nnATC Cuts at: 2906 6664 2906 Size: 3758 2912 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4477 4859 5494 275 Size: 53 83 186 936 2944 382 635 1451 Fragments arranged by size: 2944 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3378 5103 5501 5775 5922 6333 3378 Size: 1725 398 274 147 411 3715 Fragments arranged by size: 3715 1725 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 2937 2967 1178 Size: 30 165 30 912 30 592 30 4881 Fragments arranged by size: 4881 912 592 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 1186 Size: 1219 5451 BseYI C'CCAG_C Cuts at: 2419 2452 2705 2971 3547 3792 5873 6369 2419 Size: 33 253 266 576 245 2081 496 2720 Fragments arranged by size: 2720 2081 576 496 266 253 245 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6097 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4120 4881 5909 1540 Size: 275 186 2119 761 1028 2301 Fragments arranged by size: 2301 2119 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2286 3360 3456 3475 3894 4731 4816 5887 2286 Size: 1074 96 19 419 837 85 1071 3069 Fragments arranged by size: 3069 1074 1071 837 419 96 85 19 BsmI GAATG_Cn' Cuts at: 3305 3499 4181 4274 3305 Size: 194 682 93 5701 Fragments arranged by size: 5701 682 194 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2286 2445 2545 2661 2706 2889 3360 Size: 399 496 159 100 116 45 183 471 Cuts at: 3360 3456 3475 3622 3894 3989 4731 4816 5887 Size: 96 19 147 272 95 742 85 1071 Cuts at: 5887 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 496 471 399 272 183 159 147 116 100 96 95 85 45 19 BspEI T'CCGG_A Cuts at: 3378 6333 3378 Size: 2955 3715 BspHI T'CATG_A Cuts at: 3330 4559 6289 3330 Size: 1229 1730 3711 Fragments arranged by size: 3711 1730 1229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3041 4025 844 Size: 2197 984 3489 Fragments arranged by size: 3489 2197 984 BsrBI CCG'CTC Cuts at: 1394 1823 2138 3934 4557 1394 Size: 429 315 1796 623 3507 Fragments arranged by size: 3507 1796 623 429 315 BsrDI GCAATG_nn' Cuts at: 65 1248 3069 5165 5339 65 Size: 1183 1821 2096 174 1396 Fragments arranged by size: 2096 1821 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2197 5314 5501 1700 Size: 110 387 3117 187 2869 Fragments arranged by size: 3117 2869 387 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6670 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6670 BssSI C'ACGA_G Cuts at: 4730 5742 4730 Size: 1012 5658 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6670 BstBI TT'CG_AA Cuts at: 5474 5474 Size: 6670 BstEII G'GTnAC_C Cuts at: 1184 3946 1184 Size: 2762 3908 BstXI CCAn_nnnn'nTGG Cuts at: 2452 3643 3742 3925 2452 Size: 1191 99 183 5197 Fragments arranged by size: 5197 1191 183 99 BstYI r'GATC_y Cuts at: 1972 2809 3364 3381 3764 3843 4000 4367 4752 Size: 837 555 17 383 79 157 367 385 Cuts at: 4752 4769 6221 6307 6319 6665 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 837 555 385 383 367 346 157 86 79 17 17 12 BtgI C'CryG_G Cuts at: 513 1065 2881 3189 3228 3735 3786 513 Size: 552 1816 308 39 507 51 3397 Fragments arranged by size: 3397 1816 552 507 308 51 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5931 Fragments arranged by size: 5931 683 56 BtsI GCAGTG_nn' Cuts at: 3475 4186 4980 5000 3475 Size: 711 794 20 5145 Fragments arranged by size: 5145 794 711 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6670 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6635 DraI TTT'AAA Cuts at: 3974 3974 Size: 6670 DraIII CAC_nnn'GTG Cuts at: 3822 3822 Size: 6670 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5677 809 Size: 507 4361 1802 Fragments arranged by size: 4361 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2199 2637 Size: 54 1425 50 275 18 192 177 438 Cuts at: 2637 3858 4117 5006 6635 8 Size: 1221 259 889 1629 43 Fragments arranged by size: 1629 1425 1221 889 438 275 259 192 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4117 1537 Size: 275 2305 4090 Fragments arranged by size: 4090 2305 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 4600 1353 Size: 933 2314 3423 Fragments arranged by size: 3423 2314 933 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2076 2222 5257 5631 5777 387 Size: 1064 138 487 146 3035 374 146 1280 Fragments arranged by size: 3035 1280 1064 487 374 146 146 138 EcoICRI GAG'CTC Cuts at: 3454 3892 3454 Size: 438 6232 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 3363 3644 4733 5330 6117 6385 Size: 393 767 964 281 1089 597 787 268 Cuts at: 6385 1239 Size: 1524 Fragments arranged by size: 1524 1089 964 787 767 597 393 281 268 EcoNI CCTnn'n_nnAGG Cuts at: 3773 3773 Size: 6670 EcoO109I rG'GnC_Cy Cuts at: 2407 2424 2541 2764 2886 3756 2407 Size: 17 117 223 122 870 5321 Fragments arranged by size: 5321 870 223 122 117 17 EcoRI G'AATT_C Cuts at: 3979 4357 3979 Size: 378 6292 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3502 3502 Size: 6670 FspI TGC'GCA Cuts at: 5176 5176 Size: 6670 HaeII r_GCGC'y Cuts at: 1678 2084 5817 1678 Size: 406 3733 2531 Fragments arranged by size: 3733 2531 406 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2666 2698 5311 Size: 32 135 32 743 32 319 32 2613 Cuts at: 5311 5343 5385 5417 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2613 743 319 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4014 4260 669 Size: 988 281 2076 246 3079 Fragments arranged by size: 3079 2076 988 281 246 HindIII A'AGCT_T Cuts at: 748 2744 748 Size: 1996 4674 HpaI GTT'AAC Cuts at: 4260 4260 Size: 6670 KpnI G_GTAC'C Cuts at: 3057 3995 3057 Size: 938 5732 MfeI C'AATT_G Cuts at: 4269 4269 Size: 6670 MluI A'CGCG_T Cuts at: 6546 6546 Size: 6670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3575 5784 5968 1305 Size: 2270 2209 184 2007 Fragments arranged by size: 2270 2209 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4848 Fragments arranged by size: 4848 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3641 3740 4628 4987 5146 518 Size: 648 2475 99 888 359 159 2042 Fragments arranged by size: 2475 2042 888 648 359 159 99 MspA1I CmG'CkG Cuts at: 1606 2143 3529 3555 3570 3703 4086 4763 5911 Size: 537 1386 26 15 133 383 677 1148 Cuts at: 5911 6156 1606 Size: 245 2120 Fragments arranged by size: 2120 1386 1148 677 537 383 245 133 26 15 NcoI C'CATG_G Cuts at: 513 1065 2881 3189 3735 513 Size: 552 1816 308 546 3448 Fragments arranged by size: 3448 1816 552 546 308 NdeI CA'TA_TG Cuts at: 387 387 Size: 6670 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 4117 4117 Size: 6670 NsiI A_TGCA'T Cuts at: 2514 3305 2514 Size: 791 5879 NspI r_CATG'y Cuts at: 1132 1229 2512 4028 5573 1132 Size: 97 1283 1516 1545 2229 Fragments arranged by size: 2229 1545 1516 1283 97 PasI CC'CwG_GG Cuts at: 1348 2536 1348 Size: 1188 5482 PciI A'CATG_T Cuts at: 5569 5569 Size: 6670 PflMI CCAn_nnn'nTGG Cuts at: 1108 3313 1108 Size: 2205 4465 PfoI T'CCnGG_A Cuts at: 3141 3141 Size: 6670 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4739 4771 6252 6284 4739 Size: 32 1481 32 5125 Fragments arranged by size: 5125 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6670 PsiI TTA'TAA Cuts at: 4240 4240 Size: 6670 PspOMI G'GGCC_C Cuts at: 2541 2885 2541 Size: 344 6326 PstI C_TGCA'G Cuts at: 830 3174 3429 3702 3838 3870 4022 830 Size: 2344 255 273 136 32 152 3478 Fragments arranged by size: 3478 2344 273 255 152 136 32 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 PvuII CAG'CTG Cuts at: 2143 3529 2143 Size: 1386 5284 SacI G_AGCT'C Cuts at: 3456 3894 3456 Size: 438 6232 SalI G'TCGA_C Cuts at: 1936 4012 1936 Size: 2076 4594 SapI GCTCTTCn'nnn_ Cuts at: 2286 2286 Size: 6670 SbfI CC_TGCA'GG Cuts at: 4022 4022 Size: 6670 ScaI AGT'ACT Cuts at: 4918 6630 4918 Size: 1712 4958 SexAI A'CCwGG_T Cuts at: 3818 3818 Size: 6670 SfcI C'TryA_G Cuts at: 826 1045 3170 3425 3698 3834 3866 4018 5153 Size: 219 2125 255 273 136 32 152 1135 Cuts at: 5153 5834 6025 826 Size: 681 191 1471 Fragments arranged by size: 2125 1471 1135 681 273 255 219 191 152 136 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6670 SmaI CCC'GGG Cuts at: 1872 3997 1872 Size: 2125 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 2662 3751 3760 4774 5675 Size: 197 926 468 251 1089 9 1014 901 Cuts at: 5675 5937 820 Size: 262 1553 Fragments arranged by size: 1553 1089 1014 926 901 468 262 251 197 9 SnaBI TAC'GTA Cuts at: 493 4461 493 Size: 3968 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6670 SphI G_CATG'C Cuts at: 1132 2512 4028 1132 Size: 1380 1516 3774 Fragments arranged by size: 3774 1516 1380 SspI AAT'ATT Cuts at: 5 52 4594 5 Size: 47 4542 2081 Fragments arranged by size: 4542 2081 47 StuI AGG'CCT Cuts at: 1517 3923 1517 Size: 2406 4264 StyI C'CwwG_G Cuts at: 513 1065 2202 2518 2881 3189 3735 3918 3924 Size: 552 1137 316 363 308 546 183 6 Cuts at: 3924 4109 513 Size: 185 3074 Fragments arranged by size: 3074 1137 552 546 363 316 308 185 183 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2444 2492 2963 4697 4714 4867 5052 Size: 1255 864 48 471 1734 17 153 185 Cuts at: 5052 5497 325 Size: 445 1498 Fragments arranged by size: 1734 1498 1255 864 471 445 185 153 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4916 6628 96 Size: 274 80 33 51 1443 2939 1712 138 Fragments arranged by size: 2939 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2937 2969 6630 6662 2937 Size: 32 3661 32 2945 Fragments arranged by size: 3661 2945 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6670 XbaI T'CTAG_A Cuts at: 4006 4006 Size: 6670 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3196 3925 3196 Size: 729 5941 XhoI C'TCGA_G Cuts at: 1943 3751 1943 Size: 1808 4862 XmaI C'CCGG_G Cuts at: 1870 3995 1870 Size: 2125 4545 XmnI GAAnn'nnTTC Cuts at: 3690 4799 3690 Size: 1109 5561 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4478 276 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI