(Circular) (Six-base) MAPSORT of: pFN21AB4264.seq Check: 8372 from: 1 to: 6838 pFN21AB4264 6838 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4648 278 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2800 2834 4181 1937 Size: 863 34 1347 4594 Fragments arranged by size: 4594 1347 863 34 Acc65I G'GTAC_C Cuts at: 4159 4159 Size: 6838 AclI AA'CG_TT Cuts at: 1563 4965 5338 1563 Size: 3402 373 3063 Fragments arranged by size: 3402 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2142 2742 2796 3122 3459 3800 4901 6285 Size: 510 600 54 326 337 341 1101 1384 Cuts at: 6285 6553 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1101 600 510 341 337 326 268 54 AflII C'TTAA_G Cuts at: 820 1017 2012 2639 820 Size: 197 995 627 5019 Fragments arranged by size: 5019 995 627 197 AflIII A'CryG_T Cuts at: 1203 5737 6714 1203 Size: 4534 977 1327 Fragments arranged by size: 4534 1327 977 AgeI A'CCGG_T Cuts at: 5669 5669 Size: 6838 AhdI GACnn_n'nnGTC Cuts at: 5567 5567 Size: 6838 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6838 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2527 3277 1184 Size: 1343 750 4745 Fragments arranged by size: 4745 1343 750 AlwNI CAG_nnn'CTG Cuts at: 3437 4581 6153 3437 Size: 1144 1572 4122 Fragments arranged by size: 4122 1572 1144 ApaI G_GGCC'C Cuts at: 3337 3337 Size: 6838 ApaLI G'TGCA_C Cuts at: 4895 6051 4895 Size: 1156 5682 ApoI r'AATT_y Cuts at: 1493 3144 3677 3960 4002 4147 4373 4525 6634 Size: 1651 533 283 42 145 226 152 2109 Cuts at: 6634 1493 Size: 1697 Fragments arranged by size: 2109 1697 1651 533 283 226 152 145 42 AseI AT'TA_AT Cuts at: 160 1032 3531 5392 160 Size: 872 2499 1861 1606 Fragments arranged by size: 2499 1861 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 AvaI C'yCGr_G Cuts at: 1870 1943 4163 1870 Size: 73 2220 4545 Fragments arranged by size: 4545 2220 73 BamHI G'GATC_C Cuts at: 4168 4535 4168 Size: 367 6471 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4159 5614 618 Size: 325 217 627 2372 1455 1842 Fragments arranged by size: 2372 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3337 4157 1391 Size: 1946 820 4072 Fragments arranged by size: 4072 1946 820 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5867 BbvCI CC'TCA_GC Cuts at: 4091 4091 Size: 6838 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5029 5063 1903 Size: 34 3092 34 3678 Fragments arranged by size: 3678 3092 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2275 4730 5940 2275 Size: 2455 1210 3173 Fragments arranged by size: 3173 2455 1210 BclI T'GATC_A Cuts at: 1548 3826 3840 1548 Size: 2278 14 4546 Fragments arranged by size: 4546 2278 14 BfrBI ATG'CAT Cuts at: 2875 2875 Size: 6838 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3390 5449 136 Size: 107 122 71 631 605 1718 2059 1525 Fragments arranged by size: 2059 1718 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3721 6833 3721 Size: 3112 3726 BlpI GC'TnA_GC Cuts at: 2148 4255 2148 Size: 2107 4731 Bme1580I G_kGCm'C Cuts at: 1790 2077 3337 4899 6055 1790 Size: 287 1260 1562 1156 2573 Fragments arranged by size: 2573 1562 1260 1156 287 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6838 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2447 2939 4569 Size: 416 53 83 399 24 1030 492 1630 Cuts at: 4569 5527 442 Size: 958 1753 Fragments arranged by size: 1753 1630 1030 958 492 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2247 3348 5498 1239 Size: 1008 1101 2150 2579 Fragments arranged by size: 2579 2150 1101 1008 Bpu10I CC'TnA_GC Cuts at: 4091 4091 Size: 6838 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4963 5828 6126 4963 Size: 865 298 5675 Fragments arranged by size: 5675 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2471 5501 882 Size: 617 972 3030 2219 Fragments arranged by size: 3030 2219 972 617 BsaAI yAC'GTr Cuts at: 493 1204 4629 493 Size: 711 3425 2702 Fragments arranged by size: 3425 2702 711 BsaBI GATnn'nnATC Cuts at: 6832 6832 Size: 6838 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4645 5027 5662 275 Size: 53 83 186 936 3112 382 635 1451 Fragments arranged by size: 3112 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3127 5271 5669 5943 6090 6501 3127 Size: 2144 398 274 147 411 3464 Fragments arranged by size: 3464 2144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3294 3324 3735 3765 1178 Size: 30 165 30 1891 30 411 30 4251 Fragments arranged by size: 4251 1891 411 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3336 1186 Size: 2150 4688 BseYI C'CCAG_C Cuts at: 3298 3425 6041 6537 3298 Size: 127 2616 496 3599 Fragments arranged by size: 3599 2616 496 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2418 3759 1163 Size: 573 682 1341 4242 Fragments arranged by size: 4242 1341 682 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4288 5049 6077 1540 Size: 275 186 2287 761 1028 2301 Fragments arranged by size: 2301 2287 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 3475 4899 4984 6055 2028 Size: 1447 1424 85 1071 2811 Fragments arranged by size: 2811 1447 1424 1071 85 BsmI GAATG_Cn' Cuts at: 2787 4349 4442 2787 Size: 1562 93 5183 Fragments arranged by size: 5183 1562 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2077 3337 3475 4157 4899 4984 Size: 399 238 49 1260 138 682 742 85 Cuts at: 4984 6055 1391 Size: 1071 2174 Fragments arranged by size: 2174 1260 1071 742 682 399 238 138 85 49 BspEI T'CCGG_A Cuts at: 3127 6501 3127 Size: 3374 3464 BspHI T'CATG_A Cuts at: 3363 4727 6457 3363 Size: 1364 1730 3744 Fragments arranged by size: 3744 1730 1364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2519 4193 844 Size: 1675 1674 3489 Fragments arranged by size: 3489 1675 1674 BsrBI CCG'CTC Cuts at: 1394 1823 4725 1394 Size: 429 2902 3507 Fragments arranged by size: 3507 2902 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2460 5333 5507 65 Size: 1183 1212 2873 174 1396 Fragments arranged by size: 2873 1396 1212 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5482 5669 1700 Size: 110 3672 187 2869 Fragments arranged by size: 3672 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6838 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6838 BssSI C'ACGA_G Cuts at: 4898 5910 4898 Size: 1012 5826 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6838 BstBI TT'CG_AA Cuts at: 5642 5642 Size: 6838 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6838 BstXI CCAn_nnnn'nTGG Cuts at: 3115 3401 3689 3988 4035 3115 Size: 286 288 299 47 5918 Fragments arranged by size: 5918 299 288 286 47 BstYI r'GATC_y Cuts at: 1972 2765 3721 4168 4535 4920 4937 6389 6475 Size: 793 956 447 367 385 17 1452 86 Cuts at: 6475 6487 6833 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 956 793 447 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2835 2835 Size: 6838 Bsu36I CC'TnA_GG Cuts at: 2859 2859 Size: 6838 BtgI C'CryG_G Cuts at: 513 1065 2090 3585 4034 513 Size: 552 1025 1495 449 3317 Fragments arranged by size: 3317 1495 1025 552 449 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6099 Fragments arranged by size: 6099 683 56 BtsI GCAGTG_nn' Cuts at: 2430 4354 5148 5168 2430 Size: 1924 794 20 4100 Fragments arranged by size: 4100 1924 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6803 DraI TTT'AAA Cuts at: 3280 3598 4142 3280 Size: 318 544 5976 Fragments arranged by size: 5976 544 318 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5845 809 Size: 507 4529 1802 Fragments arranged by size: 4529 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2820 2851 3391 Size: 54 1425 50 275 18 990 31 540 Cuts at: 3391 4285 5174 6803 8 Size: 894 889 1629 43 Fragments arranged by size: 1629 1425 990 894 889 540 275 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 4285 1537 Size: 275 2473 4090 Fragments arranged by size: 4090 2473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2751 3600 4768 1353 Size: 1398 849 1168 3423 Fragments arranged by size: 3423 1398 1168 849 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5425 5799 5945 387 Size: 1064 138 3836 374 146 1280 Fragments arranged by size: 3836 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2247 2742 2796 3122 3348 3459 Size: 393 510 105 495 54 326 226 111 Cuts at: 3459 3800 4901 5498 6285 6553 1239 Size: 341 1101 597 787 268 1524 Fragments arranged by size: 1524 1101 787 597 510 495 393 341 326 268 226 111 105 54 EcoO109I rG'GnC_Cy Cuts at: 3292 3333 3334 3292 Size: 41 1 6796 Fragments arranged by size: 6796 41 1 EcoRI G'AATT_C Cuts at: 3677 4147 4525 3677 Size: 470 378 5990 Fragments arranged by size: 5990 470 378 EcoRV GAT'ATC Cuts at: 2659 2997 3919 2659 Size: 338 922 5578 Fragments arranged by size: 5578 922 338 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2542 2542 Size: 6838 FspI TGC'GCA Cuts at: 5344 5344 Size: 6838 HaeII r_GCGC'y Cuts at: 1678 5985 1678 Size: 4307 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2371 2403 2750 2782 2860 Size: 32 135 32 799 32 347 32 78 Cuts at: 2860 2892 3294 3326 3543 3564 3575 3596 3694 Size: 32 402 32 217 21 11 21 98 Cuts at: 3694 3726 3737 3769 5479 5511 5553 5585 1373 Size: 32 11 32 1710 32 42 32 2626 Fragments arranged by size: 2626 1710 799 402 347 217 135 98 78 42 32 32 32 32 32 32 32 32 32 32 21 21 11 11 HincII GTy'rAC Cuts at: 669 1657 1938 3873 4182 4428 669 Size: 988 281 1935 309 246 3079 Fragments arranged by size: 3079 1935 988 309 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6838 HpaI GTT'AAC Cuts at: 4428 4428 Size: 6838 KpnI G_GTAC'C Cuts at: 4163 4163 Size: 6838 MfeI C'AATT_G Cuts at: 3387 4437 3387 Size: 1050 5788 MluI A'CGCG_T Cuts at: 6714 6714 Size: 6838 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3448 5952 6136 1305 Size: 2143 2504 184 2007 Fragments arranged by size: 2504 2143 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2822 3393 10 Size: 54 1425 343 990 571 3455 Fragments arranged by size: 3455 1425 990 571 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2684 2828 3113 3584 3687 4796 5155 Size: 648 1518 144 285 471 103 1109 359 Cuts at: 5155 5314 518 Size: 159 2042 Fragments arranged by size: 2042 1518 1109 648 471 359 285 159 144 103 MspA1I CmG'CkG Cuts at: 1606 4254 4931 6079 6324 1606 Size: 2648 677 1148 245 2120 Fragments arranged by size: 2648 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2090 3585 4034 513 Size: 552 1025 1495 449 3317 Fragments arranged by size: 3317 1495 1025 552 449 NdeI CA'TA_TG Cuts at: 387 387 Size: 6838 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6838 NotI GC'GGCC_GC Cuts at: 4285 4285 Size: 6838 NsiI A_TGCA'T Cuts at: 2877 2877 Size: 6838 NspI r_CATG'y Cuts at: 1132 1229 2746 4196 5741 1132 Size: 97 1517 1450 1545 2229 Fragments arranged by size: 2229 1545 1517 1450 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6838 PciI A'CATG_T Cuts at: 5737 5737 Size: 6838 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6838 PmeI GTTT'AAAC Cuts at: 4142 4142 Size: 6838 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4907 4939 6420 6452 4907 Size: 32 1481 32 5293 Fragments arranged by size: 5293 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6838 PsiI TTA'TAA Cuts at: 2373 3150 4408 2373 Size: 777 1258 4803 Fragments arranged by size: 4803 1258 777 PspOMI G'GGCC_C Cuts at: 3333 3333 Size: 6838 PstI C_TGCA'G Cuts at: 830 3020 4190 830 Size: 2190 1170 3478 Fragments arranged by size: 3478 2190 1170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6838 SalI G'TCGA_C Cuts at: 1936 4180 1936 Size: 2244 4594 SbfI CC_TGCA'GG Cuts at: 4190 4190 Size: 6838 ScaI AGT'ACT Cuts at: 5086 6798 5086 Size: 1712 5126 SfcI C'TryA_G Cuts at: 826 1045 3016 4186 5321 6002 6193 826 Size: 219 1971 1170 1135 681 191 1471 Fragments arranged by size: 1971 1471 1170 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6838 SmaI CCC'GGG Cuts at: 1872 4165 1872 Size: 2293 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 2639 4942 5843 6105 820 Size: 197 926 69 627 2303 901 262 1553 Fragments arranged by size: 2303 1553 926 901 627 262 197 69 SnaBI TAC'GTA Cuts at: 493 4629 493 Size: 4136 2702 SpeI A'CTAG_T Cuts at: 152 2837 152 Size: 2685 4153 SphI G_CATG'C Cuts at: 1132 2746 4196 1132 Size: 1614 1450 3774 Fragments arranged by size: 3774 1614 1450 SspI AAT'ATT Cuts at: 5 52 4762 5 Size: 47 4710 2081 Fragments arranged by size: 4710 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6838 StyI C'CwwG_G Cuts at: 513 1065 2090 2290 3585 4034 4277 513 Size: 552 1025 200 1295 449 243 3074 Fragments arranged by size: 3074 1295 1025 552 449 243 200 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2950 4865 4882 5035 5220 5665 325 Size: 1255 1370 1915 17 153 185 445 1498 Fragments arranged by size: 1915 1498 1370 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 3092 3097 Size: 274 80 33 51 1443 543 572 5 Cuts at: 3097 5084 6796 96 Size: 1987 1712 138 Fragments arranged by size: 1987 1712 1443 572 543 274 138 80 51 33 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6798 6830 6798 Size: 32 6806 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6838 XbaI T'CTAG_A Cuts at: 4174 4174 Size: 6838 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6838 XmaI C'CCGG_G Cuts at: 1870 4163 1870 Size: 2293 4545 XmnI GAAnn'nnTTC Cuts at: 4967 4967 Size: 6838 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4646 276 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI