Restriction map for
pFN21AB4146
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FseI FspI
HaeII Hin1I HpaI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 604 GGTACC G^GTACC
AlwNI 1232 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 604 GGTACC G^GTACC
AvaI 55 CCCGAG C^[CT]CG[GA]G
AvaII 675 GGACC G^G[AT]CC
BalI 1521 TGGCCA TGG^CCA
BamHI 800 GGATCC G^GATCC
BanI 604 GGTACC G^G[CT][GA]CC
BciVI 1565 GGATAC GTATCC(6/5)
BclI 522 TGATCA T^GATCA
BfaI 679 CTAG C^TAG
BglII 579 AGATCT A^GATCT
BseRI 1345 CTCCTC GAGGAG(10/8)
BsgI 310 CTGCAC GTGCAG(16/14)
BsmBI 913 CGTCTC CGTCTC(1/5)
BsoBI 55 CCCGAG C^[CT]CG[GA]G
BspHI 980 TCATGA T^CATGA
CfoI 1047 GCGC GCG^C
DraII 674 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 676 GACCTAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1521 TGGCCA [CT]^GGCC[GA]
EarI 562 GAAGAG CTCTTC(1/4)
EcoO109I 674 GGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 522 TGATCA T^GATCA
HgaI 1227 GCGTC GACGC(5/10)
HhaI 1047 GCGC GCG^C
HinP1I 1047 GCGC G^CGC
HindIII 644 AAGCTT A^AGCTT
KpnI 604 GGTACC GGTAC^C
Ksp632I 562 GAAGAG CTCTTC(1/4)
MaeI 679 CTAG C^TAG
MluNI 1521 TGGCCA TGG^CCA
MscI 1521 TGGCCA TGG^CCA
MslI 1703 CATACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1308 GCATGC [GA]CATG^[CT]
PflMI 1098 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 674 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 903 CAGCTG CAG^CTG
RcaI 980 TCATGA T^CATGA
SmlI 916 CTCAAG C^T[CT][GA]AG
SphI 1308 GCATGC GCATG^C
SspI 663 AATATT AAT^ATT
TaqI 1614 TCGA T^CGA
TthHB8I 1614 TCGA T^CGA
Van91I 1098 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 1046 CGCG CG^CG
1048 CGCG
AcsI 286 GAATTT [GA]^AATT[CT]
1728 AAATTC
AfaI 3 GTAC GT^AC
605 GTAC
Alw44I 347 GTGCAC G^TGCAC
363 GTGCAC
ApaLI 347 GTGCAC G^TGCAC
363 GTGCAC
ApoI 286 GAATTT [GA]^AATT[CT]
1728 AAATTC
Asp700I 418 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1543 GAATCTGTTC
BanII 270 GGGCTC G[GA]GC[CT]^C
1142 GAGCCC
BbsI 67 GAAGAC GAAGAC(2/6)
757 GTCTTC
BcgI 315 CGACCACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1480 CGATCCATCTGC
BcnI 672 CCGGG CC^[GC]GG
1388 CCCGG
BpuAI 67 GAAGAC GAAGAC(2/6)
757 GTCTTC
BsmFI 674 GGGAC GGGAC(10/14)
892 GGGAC
BsmI 341 GAATGC GAATGC(1/-1)
1466 GCATTC
BsrDI 1175 CATTGC GCAATG(2/0)
698 GCAATG
BstUI 1046 CGCG CG^CG
1048 CGCG
BstXI 201 CCAAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
234 CCAAGAATCTGG
DraIII 687 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
971 CACCAAGTG
DsaI 471 CCATGG C^C[GA][CT]GG
795 CCATGG
EcoT22I 1310 ATGCAT ATGCA^T
1464 ATGCAT
HapII 672 CCGG C^CGG
1389 CCGG
HincII 1339 GTCAAC GT[CT]^[GA]AC
1639 GTCAAC
HindII 1339 GTCAAC GT[CT]^[GA]AC
1639 GTCAAC
HpaII 672 CCGG C^CGG
1389 CCGG
MaeII 912 ACGT A^CGT
1135 ACGT
MseI 538 TTAA T^TAA
1138 TTAA
MspA1I 903 CAGCTG C[AC]G^C[GT]G
1603 CAGCGG
MspI 672 CCGG C^CGG
1389 CCGG
MvnI 1046 CGCG CG^CG
1048 CGCG
NciI 672 CCGGG CC^[GC]GG
1388 CCCGG
NcoI 471 CCATGG C^CATGG
795 CCATGG
NsiI 1310 ATGCAT ATGCA^T
1464 ATGCAT
RsaI 3 GTAC GT^AC
605 GTAC
Tru9I 538 TTAA T^TAA
1138 TTAA
XmnI 418 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1543 GAATCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AspHI BbvI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MflI MnlI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI TseI Tsp45I
Tsp509I TspRI XcmI XhoII