(Circular) (Six-base) MAPSORT of: pFN21AB4146.seq Check: 5589 from: 1 to: 6562 pFN21AB4146 6562 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4372 278 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3905 1937 Size: 1968 4594 Acc65I G'GTAC_C Cuts at: 2609 3883 2609 Size: 1274 5288 AclI AA'CG_TT Cuts at: 1563 4689 5062 1563 Size: 3126 373 3063 Fragments arranged by size: 3126 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2414 2967 4625 6009 6277 1632 Size: 782 553 1658 1384 268 1917 Fragments arranged by size: 1917 1658 1384 782 553 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6365 AflIII A'CryG_T Cuts at: 1203 5461 6438 1203 Size: 4258 977 1327 Fragments arranged by size: 4258 1327 977 AgeI A'CCGG_T Cuts at: 5393 5393 Size: 6562 AhdI GACnn_n'nnGTC Cuts at: 5291 5291 Size: 6562 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6562 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6562 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3099 3131 3099 Size: 32 6530 AlwNI CAG_nnn'CTG Cuts at: 3242 4305 5877 3242 Size: 1063 1572 3927 Fragments arranged by size: 3927 1572 1063 ApaLI G'TGCA_C Cuts at: 2352 2368 4619 5775 2352 Size: 16 2251 1156 3139 Fragments arranged by size: 3139 2251 1156 16 ApoI r'AATT_y Cuts at: 1493 2291 3733 3871 4097 4249 6358 1493 Size: 798 1442 138 226 152 2109 1697 Fragments arranged by size: 2109 1697 1442 798 226 152 138 AseI AT'TA_AT Cuts at: 160 1032 5116 160 Size: 872 4084 1606 Fragments arranged by size: 4084 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 AvaI C'yCGr_G Cuts at: 1870 1943 2060 3887 1870 Size: 73 117 1827 4545 Fragments arranged by size: 4545 1827 117 73 BamHI G'GATC_C Cuts at: 2805 3892 4259 2805 Size: 1087 367 5108 Fragments arranged by size: 5108 1087 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2609 3883 5338 618 Size: 325 217 627 822 1274 1455 1842 Fragments arranged by size: 1842 1455 1274 822 627 325 217 BanII G_rGCy'C Cuts at: 1391 2279 3151 3881 1391 Size: 888 872 730 4072 Fragments arranged by size: 4072 888 872 730 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2079 2755 928 Size: 971 180 676 4735 Fragments arranged by size: 4735 971 676 180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2309 2343 3474 3508 4753 4787 1903 Size: 34 372 34 1131 34 1245 34 3678 Fragments arranged by size: 3678 1245 1131 372 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3564 4454 5664 3564 Size: 890 1210 4462 Fragments arranged by size: 4462 1210 890 BclI T'GATC_A Cuts at: 1548 2527 1548 Size: 979 5583 BfrBI ATG'CAT Cuts at: 3317 3471 3317 Size: 154 6408 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5173 136 Size: 107 122 71 631 605 3501 1525 Fragments arranged by size: 3501 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2584 6557 2584 Size: 3973 2589 BlpI GC'TnA_GC Cuts at: 3979 3979 Size: 6562 Bme1580I G_kGCm'C Cuts at: 1790 2356 2372 4623 5779 1790 Size: 566 16 2251 1156 2573 Fragments arranged by size: 2573 2251 1156 566 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6562 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2904 3764 4293 Size: 416 53 83 399 24 1487 860 529 Cuts at: 4293 5251 442 Size: 958 1753 Fragments arranged by size: 1753 1487 958 860 529 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6562 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2219 2219 Size: 6562 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2214 2717 3342 3600 3711 5222 1239 Size: 812 163 503 625 258 111 1511 2579 Fragments arranged by size: 2579 1511 812 625 503 258 163 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2906 4687 5552 5850 2906 Size: 1781 865 298 3618 Fragments arranged by size: 3618 1781 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5225 882 Size: 617 3726 2219 Fragments arranged by size: 3726 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4353 493 Size: 711 3149 2702 Fragments arranged by size: 3149 2702 711 BsaBI GATnn'nnATC Cuts at: 6556 6556 Size: 6562 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4369 4751 5386 275 Size: 53 83 186 936 2836 382 635 1451 Fragments arranged by size: 2836 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4995 5393 5667 5814 6225 4995 Size: 398 274 147 411 5332 Fragments arranged by size: 5332 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3573 3603 1178 Size: 30 165 30 2170 30 4137 Fragments arranged by size: 4137 2170 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3341 1186 Size: 2155 4407 BseYI C'CCAG_C Cuts at: 2727 5765 6261 2727 Size: 3038 496 3028 Fragments arranged by size: 3038 3028 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 1163 Size: 573 564 5425 Fragments arranged by size: 5425 573 564 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4012 4773 5801 1540 Size: 275 186 2011 761 1028 2301 Fragments arranged by size: 2301 2011 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2356 2372 3762 4623 4708 5779 2356 Size: 16 1390 861 85 1071 3139 Fragments arranged by size: 3139 1390 1071 861 85 16 BsmI GAATG_Cn' Cuts at: 2352 3471 4073 4166 2352 Size: 1119 602 93 4748 Fragments arranged by size: 4748 1119 602 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2924 2924 Size: 6562 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2279 2356 2372 3151 3762 3881 4623 Size: 399 489 77 16 779 611 119 742 Cuts at: 4623 4708 5779 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 779 742 611 489 399 119 85 77 16 BspEI T'CCGG_A Cuts at: 6225 6225 Size: 6562 BspHI T'CATG_A Cuts at: 2985 4451 6181 2985 Size: 1466 1730 3366 Fragments arranged by size: 3366 1730 1466 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3917 844 Size: 3073 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4449 1394 Size: 429 2626 3507 Fragments arranged by size: 3507 2626 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2710 3179 5057 5231 65 Size: 1183 1462 469 1878 174 1396 Fragments arranged by size: 1878 1462 1396 1183 469 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5206 5393 1700 Size: 110 3396 187 2869 Fragments arranged by size: 3396 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6562 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6562 BssSI C'ACGA_G Cuts at: 4622 5634 4622 Size: 1012 5550 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6562 BstBI TT'CG_AA Cuts at: 5366 5366 Size: 6562 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6562 BstXI CCAn_nnnn'nTGG Cuts at: 2213 2246 2213 Size: 33 6529 BstYI r'GATC_y Cuts at: 1972 2584 2805 3739 3892 4259 4644 4661 6113 Size: 612 221 934 153 367 385 17 1452 Cuts at: 6113 6199 6211 6557 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 934 612 385 367 346 221 153 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2476 2800 513 Size: 552 1411 324 4275 Fragments arranged by size: 4275 1411 552 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5823 Fragments arranged by size: 5823 683 56 BtsI GCAGTG_nn' Cuts at: 4078 4872 4892 4078 Size: 794 20 5748 Fragments arranged by size: 5748 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6562 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6527 DraI TTT'AAA Cuts at: 3866 3866 Size: 6562 DraIII CAC_nnn'GTG Cuts at: 2697 2981 2697 Size: 284 6278 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2687 5569 809 Size: 507 1371 2882 1802 Fragments arranged by size: 2882 1802 1371 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3526 4009 4898 Size: 54 1425 50 275 18 1696 483 889 Cuts at: 4898 6527 8 Size: 1629 43 Fragments arranged by size: 1696 1629 1425 889 483 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4009 1537 Size: 275 2197 4090 Fragments arranged by size: 4090 2197 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2562 4492 1353 Size: 1209 1930 3423 Fragments arranged by size: 3423 1930 1209 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5149 5523 5669 387 Size: 1064 138 3560 374 146 1280 Fragments arranged by size: 3560 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2214 2414 2717 2967 3342 3600 Size: 393 419 163 200 303 250 375 258 Cuts at: 3600 3711 4625 5222 6009 6277 1239 Size: 111 914 597 787 268 1524 Fragments arranged by size: 1524 914 787 597 419 393 375 303 268 258 250 200 163 111 EcoO109I rG'GnC_Cy Cuts at: 2680 2680 Size: 6562 EcoRI G'AATT_C Cuts at: 3871 4249 3871 Size: 378 6184 FspI TGC'GCA Cuts at: 5068 5068 Size: 6562 HaeII r_GCGC'y Cuts at: 1678 5709 1678 Size: 4031 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2797 2829 3533 3565 5203 Size: 32 135 32 1225 32 704 32 1638 Cuts at: 5203 5235 5277 5309 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1638 1225 704 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3346 3646 3906 4152 669 Size: 988 281 1408 300 260 246 3079 Fragments arranged by size: 3079 1408 988 300 281 260 246 HindIII A'AGCT_T Cuts at: 748 2649 748 Size: 1901 4661 HpaI GTT'AAC Cuts at: 4152 4152 Size: 6562 KpnI G_GTAC'C Cuts at: 2613 3887 2613 Size: 1274 5288 MfeI C'AATT_G Cuts at: 4161 4161 Size: 6562 MluI A'CGCG_T Cuts at: 6438 6438 Size: 6562 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2838 5676 5860 1305 Size: 1533 2838 184 2007 Fragments arranged by size: 2838 2007 1533 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3528 10 Size: 54 1425 343 1696 3044 Fragments arranged by size: 3044 1696 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3712 4520 4879 5038 518 Size: 648 2546 808 359 159 2042 Fragments arranged by size: 2546 2042 808 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2910 3610 3978 4655 5803 6048 1606 Size: 1304 700 368 677 1148 245 2120 Fragments arranged by size: 2120 1304 1148 700 677 368 245 NcoI C'CATG_G Cuts at: 513 1065 2476 2800 513 Size: 552 1411 324 4275 Fragments arranged by size: 4275 1411 552 324 NdeI CA'TA_TG Cuts at: 387 387 Size: 6562 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6562 NotI GC'GGCC_GC Cuts at: 4009 4009 Size: 6562 NsiI A_TGCA'T Cuts at: 3319 3473 3319 Size: 154 6408 NspI r_CATG'y Cuts at: 1132 1229 3317 3920 5465 1132 Size: 97 2088 603 1545 2229 Fragments arranged by size: 2229 2088 1545 603 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6562 PciI A'CATG_T Cuts at: 5461 5461 Size: 6562 PflMI CCAn_nnn'nTGG Cuts at: 1108 3109 1108 Size: 2001 4561 PfoI T'CCnGG_A Cuts at: 3392 3392 Size: 6562 PmeI GTTT'AAAC Cuts at: 3866 3866 Size: 6562 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4631 4663 6144 6176 4631 Size: 32 1481 32 5017 Fragments arranged by size: 5017 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2680 2680 Size: 6562 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6562 PsiI TTA'TAA Cuts at: 3414 4132 3414 Size: 718 5844 PstI C_TGCA'G Cuts at: 830 3914 830 Size: 3084 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6562 PvuII CAG'CTG Cuts at: 2910 2910 Size: 6562 SalI G'TCGA_C Cuts at: 1936 3904 1936 Size: 1968 4594 SbfI CC_TGCA'GG Cuts at: 3914 3914 Size: 6562 ScaI AGT'ACT Cuts at: 4810 6522 4810 Size: 1712 4850 SfcI C'TryA_G Cuts at: 826 1045 2297 3131 3304 3910 5045 5726 5917 Size: 219 1252 834 173 606 1135 681 191 Cuts at: 5917 826 Size: 1471 Fragments arranged by size: 1471 1252 1135 834 681 606 219 191 173 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6562 SmaI CCC'GGG Cuts at: 1872 3889 1872 Size: 2017 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2921 4666 5567 5829 820 Size: 197 926 978 1745 901 262 1553 Fragments arranged by size: 1745 1553 978 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4353 493 Size: 3860 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6562 SphI G_CATG'C Cuts at: 1132 3317 3920 1132 Size: 2185 603 3774 Fragments arranged by size: 3774 2185 603 SspI AAT'ATT Cuts at: 5 52 2670 4486 5 Size: 47 2618 1816 2081 Fragments arranged by size: 2618 2081 1816 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6562 StyI C'CwwG_G Cuts at: 513 1065 2476 2800 3504 4001 513 Size: 552 1411 324 704 497 3074 Fragments arranged by size: 3074 1411 704 552 497 324 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3116 4589 4606 4759 4944 5389 325 Size: 1255 1536 1473 17 153 185 445 1498 Fragments arranged by size: 1536 1498 1473 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4808 6520 96 Size: 274 80 33 51 1443 2831 1712 138 Fragments arranged by size: 2831 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2457 2489 6522 6554 2457 Size: 32 4033 32 2465 Fragments arranged by size: 4033 2465 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6562 XbaI T'CTAG_A Cuts at: 3898 3898 Size: 6562 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2735 3496 3804 2735 Size: 761 308 5493 Fragments arranged by size: 5493 761 308 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6562 XmaI C'CCGG_G Cuts at: 1870 3887 1870 Size: 2017 4545 XmnI GAAnn'nnTTC Cuts at: 2427 3552 4691 2427 Size: 1125 1139 4298 Fragments arranged by size: 4298 1139 1125 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4370 276 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI