Restriction map for
pFN21AB4146
(Linear) (Six-base) MAPSORT of: KIBB4146.seq Check: 5012 from: 1 to: 1857
KIBB4146.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
Acc65I G'GTAC_C
Cuts at: 0 604 1857
Size: 604 1253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 962 1857
Size: 409 553 895
Fragments arranged by size:
895 553 409
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1094 1126 1857
Size: 1094 32 731
Fragments arranged by size:
1094 731 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1237 1857
Size: 1237 620
ApaLI G'TGCA_C
Cuts at: 0 347 363 1857
Size: 347 16 1494
Fragments arranged by size:
1494 347 16
ApoI r'AATT_y
Cuts at: 0 286 1728 1857
Size: 286 1442 129
Fragments arranged by size:
1442 286 129
AvaI C'yCGr_G
Cuts at: 0 55 1857
Size: 55 1802
BamHI G'GATC_C
Cuts at: 0 800 1857
Size: 800 1057
BanI G'GyrC_C
Cuts at: 0 604 1857
Size: 604 1253
BanII G_rGCy'C
Cuts at: 0 274 1146 1857
Size: 274 872 711
Fragments arranged by size:
872 711 274
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 750 1857
Size: 74 676 1107
Fragments arranged by size:
1107 676 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 304 338 1469 1503 1857
Size: 304 34 1131 34 354
Fragments arranged by size:
1131 354 304 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1559 1857
Size: 1559 298
BclI T'GATC_A
Cuts at: 0 522 1857
Size: 522 1335
BfrBI ATG'CAT
Cuts at: 0 1312 1466 1857
Size: 1312 154 391
Fragments arranged by size:
1312 391 154
BglII A'GATC_T
Cuts at: 0 579 1857
Size: 579 1278
Bme1580I G_kGCm'C
Cuts at: 0 351 367 1857
Size: 351 16 1490
Fragments arranged by size:
1490 351 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 899 1759 1857
Size: 899 860 98
Fragments arranged by size:
899 860 98
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 214 1857
Size: 214 1643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 209 712 1337 1595 1706 1857
Size: 46 163 503 625 258 111 151
Fragments arranged by size:
625 503 258 163 151 111 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1857
Size: 901 956
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1568 1598 1857
Size: 1568 30 259
Fragments arranged by size:
1568 259 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1336 1857
Size: 1336 521
BseYI C'CCAG_C
Cuts at: 0 722 1857
Size: 722 1135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1857
Size: 295 1562
BsiHKAI G_wGCw'C
Cuts at: 0 351 367 1757 1857
Size: 351 16 1390 100
Fragments arranged by size:
1390 351 100 16
BsmI GAATG_Cn'
Cuts at: 0 347 1466 1857
Size: 347 1119 391
Fragments arranged by size:
1119 391 347
BsmBI CGTCTCn'nnnn_
Cuts at: 0 919 1857
Size: 919 938
Bsp1286I G_dGCh'C
Cuts at: 0 274 351 367 1146 1757 1857
Size: 274 77 16 779 611 100
Fragments arranged by size:
779 611 274 100 77 16
BspHI T'CATG_A
Cuts at: 0 980 1857
Size: 980 877
BsrDI GCAATG_nn'
Cuts at: 0 705 1174 1857
Size: 705 469 683
Fragments arranged by size:
705 683 469
BstXI CCAn_nnnn'nTGG
Cuts at: 0 208 241 1857
Size: 208 33 1616
Fragments arranged by size:
1616 208 33
BstYI r'GATC_y
Cuts at: 0 579 800 1734 1857
Size: 579 221 934 123
Fragments arranged by size:
934 579 221 123
BtgI C'CryG_G
Cuts at: 0 471 795 1857
Size: 471 324 1062
Fragments arranged by size:
1062 471 324
DraIII CAC_nnn'GTG
Cuts at: 0 692 976 1857
Size: 692 284 881
Fragments arranged by size:
881 692 284
DrdI GACnn_nn'nnGTC
Cuts at: 0 682 1857
Size: 682 1175
EaeI y'GGCC_r
Cuts at: 0 1521 1857
Size: 1521 336
EarI CTCTTCn'nnn_
Cuts at: 0 557 1857
Size: 557 1300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 209 409 712 962 1337 1595 1706
Size: 46 163 200 303 250 375 258 111
Cuts at: 1706 1857
Size: 151
Fragments arranged by size:
375 303 258 250 200 163 151 111
46
EcoO109I rG'GnC_Cy
Cuts at: 0 675 1857
Size: 675 1182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 792 824 1528 1560 1857
Size: 792 32 704 32 297
Fragments arranged by size:
792 704 297 32 32
HincII GTy'rAC
Cuts at: 0 1341 1641 1857
Size: 1341 300 216
Fragments arranged by size:
1341 300 216
HindIII A'AGCT_T
Cuts at: 0 644 1857
Size: 644 1213
KpnI G_GTAC'C
Cuts at: 0 608 1857
Size: 608 1249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 1857
Size: 833 1024
MscI TGG'CCA
Cuts at: 0 1523 1857
Size: 1523 334
MslI CAynn'nnrTG
Cuts at: 0 1707 1857
Size: 1707 150
MspA1I CmG'CkG
Cuts at: 0 905 1605 1857
Size: 905 700 252
Fragments arranged by size:
905 700 252
NcoI C'CATG_G
Cuts at: 0 471 795 1857
Size: 471 324 1062
Fragments arranged by size:
1062 471 324
NsiI A_TGCA'T
Cuts at: 0 1314 1468 1857
Size: 1314 154 389
Fragments arranged by size:
1314 389 154
NspI r_CATG'y
Cuts at: 0 1312 1857
Size: 1312 545
PflMI CCAn_nnn'nTGG
Cuts at: 0 1104 1857
Size: 1104 753
PfoI T'CCnGG_A
Cuts at: 0 1387 1857
Size: 1387 470
PpuMI rG'GwC_Cy
Cuts at: 0 675 1857
Size: 675 1182
PsiI TTA'TAA
Cuts at: 0 1409 1857
Size: 1409 448
PvuII CAG'CTG
Cuts at: 0 905 1857
Size: 905 952
SfcI C'TryA_G
Cuts at: 0 292 1126 1299 1857
Size: 292 834 173 558
Fragments arranged by size:
834 558 292 173
SmlI C'TyrA_G
Cuts at: 0 916 1857
Size: 916 941
SphI G_CATG'C
Cuts at: 0 1312 1857
Size: 1312 545
SspI AAT'ATT
Cuts at: 0 665 1857
Size: 665 1192
StyI C'CwwG_G
Cuts at: 0 471 795 1499 1857
Size: 471 324 704 358
Fragments arranged by size:
704 471 358 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1111 1857
Size: 1111 746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 452 484 1857
Size: 452 32 1373
Fragments arranged by size:
1373 452 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 730 1491 1799 1857
Size: 730 761 308 58
Fragments arranged by size:
761 730 308 58
XmnI GAAnn'nnTTC
Cuts at: 0 422 1547 1857
Size: 422 1125 310
Fragments arranged by size:
1125 422 310
Enzymes that do cut:
Acc65I AcuI AloI AlwNI ApaLI ApoI AvaI BamHI
BanI BanII BbsI BcgI BciVI BclI BfrBI BglII
Bme1580I BmrI BplI BpmI BpuEI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BstXI
BstYI BtgI DraIII DrdI EaeI EarI Eco57MI EcoO109I
Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I
NcoI NsiI NspI PflMI PfoI PpuMI PsiI PvuII
SfcI SmlI SphI SspI StyI TaqII TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BglI BlpI BmgBI BmtI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI TatI Tth111I XbaI
XhoI XmaI ZraI