Restriction map for
pFN21AB3518
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BfrI BlnI BsaAI
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstAPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI ScaI SfiI SfoI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tsp509I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1174 GACGTC GACGT^C
AfaI 540 GTAC GT^AC
AhdI 577 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 764 AGCGCT AGC^GCT
AspEI 577 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2264 TGGCCA TGG^CCA
BglI 655 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1230 AGATCT A^GATCT
BlpI 1626 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1626 GCTGAGC GC^T[ACGT]AGC
BsaI 99 GGTCTC GGTCTC(1/5)
BsmBI 2131 CGTCTC CGTCTC(1/5)
BsrDI 2238 CATTGC GCAATG(2/0)
BsrFI 2031 GCCGGT [GA]^CCGG[CT]
BstBI 235 TTCGAA TT^CGAA
BstEII 483 GGTCACC G^GT[ACGT]ACC
BstPI 483 GGTCACC G^GT[ACGT]ACC
CelII 1626 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2031 GCCGGT [GA]^CCGG[CT]
DraIII 1834 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 17 CGGCCG C^GGCCG
Eam1105I 577 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 547 GAAGAG CTCTTC(1/4)
EclXI 17 CGGCCG C^GGCCG
Eco47III 764 AGCGCT AGC^GCT
Eco52I 17 CGGCCG C^GGCCG
EcoO65I 483 GGTCACC G^GT[ACGT]ACC
Ksp632I 547 GAAGAG CTCTTC(1/4)
KspI 23 CCGCGG CCGC^GG
MluNI 2264 TGGCCA TGG^CCA
MscI 2264 TGGCCA TGG^CCA
MseI 1292 TTAA T^TAA
MslI 1800 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2042 CCATGG C^CATGG
NotI 16 GCGGCCGC GC^GGCCGC
NspV 235 TTCGAA TT^CGAA
PvuII 1260 CAGCTG CAG^CTG
RsaI 540 GTAC GT^AC
SacI 622 GAGCTC GAGCT^C
SacII 23 CCGCGG CCGC^GG
SbfI 676 CCTGCAGG CCTGCA^GG
SexAI 338 ACCTGGT A^CC[AT]GGT
SfuI 235 TTCGAA TT^CGAA
SmlI 1405 CTCAAG C^T[CT][GA]AG
Sse8387I 676 CCTGCAGG CCTGCA^GG
Tru9I 1292 TTAA T^TAA
XcmI 464 CCAATGGCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 1174 GACGTC G[GA]^CG[CT]C
1654 GACGCC
Alw44I 1008 GTGCAC G^TGCAC
2035 GTGCAC
ApaLI 1008 GTGCAC G^TGCAC
2035 GTGCAC
AspI 1861 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2059 GACGCAGTC
BamHI 200 GGATCC G^GATCC
1596 GGATCC
BanI 897 GGTGCC G^G[CT][GA]CC
1717 GGCACC
BbvCI 1257 CCTCAGC CCTCAGC(-5/-2)
1587 CCTCAGC
BsaHI 1174 GACGTC G[GA]^CG[CT]C
1654 GACGCC
BsiEI 17 CGGCCG CG[GA][CT]^CG
26 CGGTCG
BssHII 229 GCGCGC G^CGCGC
267 GCGCGC
BssSI 220 CACGAG CACGAG(-5/-1)
2191 CACGAG
BstXI 887 CCAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1799 CCACCTCAGTGG
Hin1I 1174 GACGTC G[GA]^CG[CT]C
1654 GACGCC
NspI 578 ACATGC [GA]CATG^[CT]
1473 ACATGC
PflFI 1861 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2059 GACGCAGTC
PpuMI 1733 GGGACCC [GA]G^G[AT]CC[CT]
1793 GGGTCCC
Tth111I 1861 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2059 GACGCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI