(Circular) (Six-base) MAPSORT of: pFN21AB3518.seq Check: 5326 from: 1 to: 6985 pFN21AB3518 6985 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4082 4082 Size: 6985 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3183 4795 278 Size: 53 83 186 936 1647 1612 2468 Fragments arranged by size: 2468 1647 1612 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4328 1937 Size: 2391 4594 Acc65I G'GTAC_C Cuts at: 4306 4306 Size: 6985 AclI AA'CG_TT Cuts at: 1563 5112 5485 1563 Size: 3549 373 3063 Fragments arranged by size: 3549 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2288 2399 2429 3664 5048 6432 6700 1632 Size: 656 111 30 1235 1384 1384 268 1917 Fragments arranged by size: 1917 1384 1384 1235 656 268 111 30 AfeI AGC'GCT Cuts at: 2771 2771 Size: 6985 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6788 AflIII A'CryG_T Cuts at: 1203 5884 6861 1203 Size: 4681 977 1327 Fragments arranged by size: 4681 1327 977 AgeI A'CCGG_T Cuts at: 5816 5816 Size: 6985 AhdI GACnn_n'nnGTC Cuts at: 2587 5714 2587 Size: 3127 3858 AleI CACnn'nnGTG Cuts at: 1166 3809 1166 Size: 2643 4342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3580 4090 1184 Size: 2396 510 4079 Fragments arranged by size: 4079 2396 510 AlwNI CAG_nnn'CTG Cuts at: 2548 2681 3700 4728 6300 2548 Size: 133 1019 1028 1572 3233 Fragments arranged by size: 3233 1572 1028 1019 133 ApaI G_GGCC'C Cuts at: 2081 2132 2172 2183 2662 3503 2081 Size: 51 40 11 479 841 5563 Fragments arranged by size: 5563 841 479 51 40 11 ApaLI G'TGCA_C Cuts at: 3013 4040 5042 6198 3013 Size: 1027 1002 1156 3800 Fragments arranged by size: 3800 1156 1027 1002 ApoI r'AATT_y Cuts at: 1493 4294 4520 4672 6781 1493 Size: 2801 226 152 2109 1697 Fragments arranged by size: 2801 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5539 160 Size: 872 4507 1606 Fragments arranged by size: 4507 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 AvaI C'yCGr_G Cuts at: 1870 1943 2120 2165 3918 4310 1870 Size: 73 177 45 1753 392 4545 Fragments arranged by size: 4545 1753 392 177 73 45 BamHI G'GATC_C Cuts at: 2205 3601 4315 4682 2205 Size: 1396 714 367 4508 Fragments arranged by size: 4508 1396 714 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2902 3722 4306 5761 618 Size: 325 217 627 1115 820 584 1455 1842 Fragments arranged by size: 1842 1455 1115 820 627 584 325 217 BanII G_rGCy'C Cuts at: 1391 2081 2132 2172 2183 2190 2631 2662 2671 Size: 690 51 40 11 7 441 31 9 Cuts at: 2671 2691 2975 2998 3032 3493 3503 4304 1391 Size: 20 284 23 34 461 10 801 4072 Fragments arranged by size: 4072 801 690 461 441 284 51 40 34 31 23 20 11 10 9 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2832 2922 2937 3175 3308 4270 928 Size: 971 933 90 15 238 133 962 3643 Fragments arranged by size: 3643 971 962 933 238 133 90 15 BbvCI CC'TCA_GC Cuts at: 3263 3593 3263 Size: 330 6655 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5176 5210 1903 Size: 34 3239 34 3678 Fragments arranged by size: 3678 3239 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4877 6087 4877 Size: 1210 5775 BclI T'GATC_A Cuts at: 1548 1548 Size: 6985 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2666 5596 136 Size: 107 122 71 631 605 994 2930 1525 Fragments arranged by size: 2930 1525 994 631 605 122 107 71 BglII A'GATC_T Cuts at: 3235 6980 3235 Size: 3745 3240 BlpI GC'TnA_GC Cuts at: 3632 4402 3632 Size: 770 6215 Bme1580I G_kGCm'C Cuts at: 1790 2081 2132 2172 2183 2662 2907 3017 3503 Size: 291 51 40 11 479 245 110 486 Cuts at: 3503 3901 4044 5046 6202 1790 Size: 398 143 1002 1156 2573 Fragments arranged by size: 2573 1156 1002 486 479 398 291 245 143 110 51 40 11 BmgBI CAC'GTC Cuts at: 1318 3306 3640 1318 Size: 1988 334 4663 Fragments arranged by size: 4663 1988 334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2100 3497 4716 Size: 416 53 83 399 24 683 1397 1219 Cuts at: 4716 5674 442 Size: 958 1753 Fragments arranged by size: 1753 1397 1219 958 683 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6985 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2516 2645 2982 3665 3932 4162 5645 1239 Size: 1277 129 337 683 267 230 1483 2579 Fragments arranged by size: 2579 1483 1277 683 337 267 230 129 Bpu10I CC'TnA_GC Cuts at: 2068 2192 2289 2974 3263 3593 2068 Size: 124 97 685 289 330 5460 Fragments arranged by size: 5460 685 330 289 124 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3395 5110 5975 6273 3395 Size: 1715 865 298 4107 Fragments arranged by size: 4107 1715 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2110 5648 882 Size: 617 611 3538 2219 Fragments arranged by size: 3538 2219 617 611 BsaAI yAC'GTr Cuts at: 493 1204 4776 493 Size: 711 3572 2702 Fragments arranged by size: 3572 2702 711 BsaBI GATnn'nnATC Cuts at: 6979 6979 Size: 6985 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3180 3660 4792 5174 Size: 53 83 186 936 1647 480 1132 382 Cuts at: 5174 5809 275 Size: 635 1451 Fragments arranged by size: 1647 1451 1132 936 635 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5418 5816 6090 6237 6648 5418 Size: 398 274 147 411 5755 Fragments arranged by size: 5755 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6760 Fragments arranged by size: 6760 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2038 2741 3163 3893 4214 1186 Size: 841 11 703 422 730 321 3957 Fragments arranged by size: 3957 841 730 703 422 321 11 BseYI C'CCAG_C Cuts at: 2259 2611 2788 3138 3693 3743 4229 6188 6684 Size: 352 177 350 555 50 486 1959 496 Cuts at: 6684 2259 Size: 2560 Fragments arranged by size: 2560 1959 555 496 486 352 350 177 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2195 2879 3754 4187 1163 Size: 573 459 684 875 433 3961 Fragments arranged by size: 3961 875 684 573 459 433 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 2034 4435 5196 6224 1540 Size: 275 186 24 9 2401 761 1028 2301 Fragments arranged by size: 2401 2301 1028 761 275 186 24 9 BsiHKAI G_wGCw'C Cuts at: 2631 3017 3795 4044 5046 5131 6202 2631 Size: 386 778 249 1002 85 1071 3414 Fragments arranged by size: 3414 1071 1002 778 386 249 85 BsmI GAATG_Cn' Cuts at: 3069 3345 3457 4100 4496 4589 3069 Size: 276 112 643 396 93 5465 Fragments arranged by size: 5465 643 396 276 112 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4142 4142 Size: 6985 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2132 2172 2183 2190 2631 2662 Size: 399 291 51 40 11 7 441 31 Cuts at: 2662 2671 2691 2907 2975 2998 3017 3032 3493 Size: 9 20 216 68 23 19 15 461 Cuts at: 3493 3503 3795 3901 4044 4304 5046 5131 6202 Size: 10 292 106 143 260 742 85 1071 Cuts at: 6202 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 461 441 399 292 291 260 216 143 106 85 68 51 40 31 23 20 19 15 11 10 9 7 BspEI T'CCGG_A Cuts at: 6648 6648 Size: 6985 BspHI T'CATG_A Cuts at: 4874 6604 4874 Size: 1730 5255 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2126 3090 3432 4082 4340 844 Size: 1282 964 342 650 258 3489 Fragments arranged by size: 3489 1282 964 650 342 258 BsrBI CCG'CTC Cuts at: 1394 1823 2021 2062 2619 3858 4872 1394 Size: 429 198 41 557 1239 1014 3507 Fragments arranged by size: 3507 1239 1014 557 429 198 41 BsrDI GCAATG_nn' Cuts at: 65 1248 4242 5480 5654 65 Size: 1183 2994 1238 174 1396 Fragments arranged by size: 2994 1396 1238 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4036 5629 5816 1700 Size: 110 2226 1593 187 2869 Fragments arranged by size: 2869 2226 1593 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6985 BssHII G'CGCG_C Cuts at: 1925 2234 2272 1925 Size: 309 38 6638 Fragments arranged by size: 6638 309 38 BssSI C'ACGA_G Cuts at: 2225 4196 5045 6057 2225 Size: 1971 849 1012 3153 Fragments arranged by size: 3153 1971 1012 849 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6985 BstBI TT'CG_AA Cuts at: 2241 5789 2241 Size: 3548 3437 BstEII G'GTnAC_C Cuts at: 1184 2488 1184 Size: 1304 5681 BstXI CCAn_nnnn'nTGG Cuts at: 2899 3811 2899 Size: 912 6073 BstYI r'GATC_y Cuts at: 1972 2205 2796 3235 3601 4315 4682 5067 5084 Size: 233 591 439 366 714 367 385 17 Cuts at: 5084 6536 6622 6634 6980 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 714 591 439 385 367 366 346 233 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2028 2172 2478 3652 4047 4083 513 Size: 552 963 144 306 1174 395 36 3415 Fragments arranged by size: 3415 1174 963 552 395 306 144 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6246 Fragments arranged by size: 6246 683 56 BtsI GCAGTG_nn' Cuts at: 2646 3901 4501 5295 5315 2646 Size: 1255 600 794 20 4316 Fragments arranged by size: 4316 1255 794 600 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6985 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6950 DraI TTT'AAA Cuts at: 4289 4289 Size: 6985 DraIII CAC_nnn'GTG Cuts at: 3844 3844 Size: 6985 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5992 809 Size: 507 4676 1802 Fragments arranged by size: 4676 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 3146 3441 Size: 54 1425 50 275 18 192 1124 295 Cuts at: 3441 4269 4432 5321 6950 8 Size: 828 163 889 1629 43 Fragments arranged by size: 1629 1425 1124 889 828 295 275 192 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2022 4432 1537 Size: 275 210 2410 4090 Fragments arranged by size: 4090 2410 275 210 EarI CTCTTCn'nnn_ Cuts at: 1353 2547 4915 1353 Size: 1194 2368 3423 Fragments arranged by size: 3423 2368 1194 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2098 2169 2368 5572 5946 6092 Size: 1064 138 509 71 199 3204 374 146 Cuts at: 6092 387 Size: 1280 Fragments arranged by size: 3204 1280 1064 509 374 199 146 138 71 EcoICRI GAG'CTC Cuts at: 2629 2629 Size: 6985 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2399 2429 2516 2645 2982 3664 Size: 393 656 111 30 87 129 337 682 Cuts at: 3664 3665 3932 4162 5048 5645 6432 6700 1239 Size: 1 267 230 886 597 787 268 1524 Fragments arranged by size: 1524 886 787 682 656 597 393 337 268 267 230 129 111 87 30 1 EcoNI CCTnn'n_nnAGG Cuts at: 2429 2552 3428 4271 2429 Size: 123 876 843 5143 Fragments arranged by size: 5143 876 843 123 EcoO109I rG'GnC_Cy Cuts at: 2078 2658 2659 2678 3499 3670 3739 3799 4237 Size: 580 1 19 821 171 69 60 438 Cuts at: 4237 2078 Size: 4826 Fragments arranged by size: 4826 821 580 438 171 69 60 19 1 EcoRI G'AATT_C Cuts at: 4294 4672 4294 Size: 378 6607 FspI TGC'GCA Cuts at: 5491 5491 Size: 6985 HaeII r_GCGC'y Cuts at: 1678 2773 3461 3700 4260 6132 1678 Size: 1095 688 239 560 1872 2531 Fragments arranged by size: 2531 1872 1095 688 560 239 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2573 2605 5626 5658 5700 Size: 32 135 32 1001 32 3021 32 42 Cuts at: 5700 5732 1373 Size: 32 2626 Fragments arranged by size: 3021 2626 1001 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4329 4575 669 Size: 988 281 2391 246 3079 Fragments arranged by size: 3079 2391 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6985 HpaI GTT'AAC Cuts at: 4575 4575 Size: 6985 KpnI G_GTAC'C Cuts at: 4310 4310 Size: 6985 MfeI C'AATT_G Cuts at: 4584 4584 Size: 6985 MluI A'CGCG_T Cuts at: 6861 6861 Size: 6985 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2563 3557 3959 6099 6283 1305 Size: 1258 994 402 2140 184 2007 Fragments arranged by size: 2140 2007 1258 994 402 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4271 10 Size: 54 1425 343 2439 2724 Fragments arranged by size: 2724 2439 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3809 4943 5302 5461 518 Size: 648 2643 1134 359 159 2042 Fragments arranged by size: 2643 2042 1134 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2259 3267 3930 4401 5078 6226 6471 Size: 424 229 1008 663 471 677 1148 245 Cuts at: 6471 1606 Size: 2120 Fragments arranged by size: 2120 1148 1008 677 663 471 424 245 229 NcoI C'CATG_G Cuts at: 513 1065 4047 513 Size: 552 2982 3451 Fragments arranged by size: 3451 2982 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6985 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6985 NotI GC'GGCC_GC Cuts at: 2022 4432 2022 Size: 2410 4575 NspI r_CATG'y Cuts at: 1132 1229 2587 3482 4343 5888 1132 Size: 97 1358 895 861 1545 2229 Fragments arranged by size: 2229 1545 1358 895 861 97 PasI CC'CwG_GG Cuts at: 1348 2664 1348 Size: 1316 5669 PciI A'CATG_T Cuts at: 5884 5884 Size: 6985 PflMI CCAn_nnn'nTGG Cuts at: 1108 3040 3643 3700 3844 1108 Size: 1932 603 57 144 4249 Fragments arranged by size: 4249 1932 603 144 57 PfoI T'CCnGG_A Cuts at: 3642 4014 3642 Size: 372 6613 PmeI GTTT'AAAC Cuts at: 4289 4289 Size: 6985 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5054 5086 6567 6599 5054 Size: 32 1481 32 5440 Fragments arranged by size: 5440 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3739 3799 3739 Size: 60 6925 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6985 PsiI TTA'TAA Cuts at: 4555 4555 Size: 6985 PspOMI G'GGCC_C Cuts at: 2077 2128 2168 2179 2658 3499 2077 Size: 51 40 11 479 841 5563 Fragments arranged by size: 5563 841 479 51 40 11 PstI C_TGCA'G Cuts at: 830 2686 2811 3429 4337 830 Size: 1856 125 618 908 3478 Fragments arranged by size: 3478 1856 908 618 125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6985 PvuII CAG'CTG Cuts at: 3267 3267 Size: 6985 SacI G_AGCT'C Cuts at: 2631 2631 Size: 6985 SacII CC_GC'GG Cuts at: 2031 2031 Size: 6985 SalI G'TCGA_C Cuts at: 1936 4327 1936 Size: 2391 4594 SanDI GG'GwC_CC Cuts at: 3739 3799 3739 Size: 60 6925 SbfI CC_TGCA'GG Cuts at: 2686 4337 2686 Size: 1651 5334 ScaI AGT'ACT Cuts at: 5233 6945 5233 Size: 1712 5273 SexAI A'CCwGG_T Cuts at: 2343 2343 Size: 6985 SfcI C'TryA_G Cuts at: 826 1045 2682 2807 3425 3544 4333 5468 6149 Size: 219 1637 125 618 119 789 1135 681 Cuts at: 6149 6340 826 Size: 191 1471 Fragments arranged by size: 1637 1471 1135 789 681 618 219 191 125 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6985 SmaI CCC'GGG Cuts at: 1872 2122 2167 3920 4312 1872 Size: 250 45 1753 392 4545 Fragments arranged by size: 4545 1753 392 250 45 SmlI C'TyrA_G Cuts at: 820 1017 1943 3410 5089 5990 6252 820 Size: 197 926 1467 1679 901 262 1553 Fragments arranged by size: 1679 1553 1467 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4776 493 Size: 4283 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6985 SphI G_CATG'C Cuts at: 1132 4343 1132 Size: 3211 3774 SspI AAT'ATT Cuts at: 5 52 4909 5 Size: 47 4857 2081 Fragments arranged by size: 4857 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6985 StyI C'CwwG_G Cuts at: 513 1065 2892 3064 4047 4272 4424 513 Size: 552 1827 172 983 225 152 3074 Fragments arranged by size: 3074 1827 983 552 225 172 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2476 5012 5029 5182 5367 5812 325 Size: 1255 896 2536 17 153 185 445 1498 Fragments arranged by size: 2536 1498 1255 896 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5231 6943 96 Size: 274 80 33 51 1443 3254 1712 138 Fragments arranged by size: 3254 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3650 3682 6945 6977 3650 Size: 32 3263 32 3658 Fragments arranged by size: 3658 3263 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3869 4067 1316 Size: 2553 198 4234 Fragments arranged by size: 4234 2553 198 XbaI T'CTAG_A Cuts at: 4321 4321 Size: 6985 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 6985 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6985 XmaI C'CCGG_G Cuts at: 1870 2120 2165 3918 4310 1870 Size: 250 45 1753 392 4545 Fragments arranged by size: 4545 1753 392 250 45 XmnI GAAnn'nnTTC Cuts at: 5114 5114 Size: 6985 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3181 4793 276 Size: 53 83 186 936 1647 1612 2468 Fragments arranged by size: 2468 1647 1612 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI