Restriction map for
pFN21AB3518
(Linear) (Six-base) MAPSORT of: KIBB3518.seq Check: 1742 from: 1 to: 2280
KIBB3518.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2077 2280
Size: 2077 203
AatII G_ACGT'C
Cuts at: 0 1178 2280
Size: 1178 1102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 394 424 1659 2280
Size: 283 111 30 1235 621
Fragments arranged by size:
1235 621 283 111 30
AfeI AGC'GCT
Cuts at: 0 766 2280
Size: 766 1514
AhdI GACnn_n'nnGTC
Cuts at: 0 582 2280
Size: 582 1698
AleI CACnn'nnGTG
Cuts at: 0 1804 2280
Size: 1804 476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1575 2085 2280
Size: 1575 510 195
Fragments arranged by size:
1575 510 195
AlwNI CAG_nnn'CTG
Cuts at: 0 543 676 1695 2280
Size: 543 133 1019 585
Fragments arranged by size:
1019 585 543 133
ApaI G_GGCC'C
Cuts at: 0 76 127 167 178 657 1498 2280
Size: 76 51 40 11 479 841 782
Fragments arranged by size:
841 782 479 76 51 40 11
ApaLI G'TGCA_C
Cuts at: 0 1008 2035 2280
Size: 1008 1027 245
Fragments arranged by size:
1027 1008 245
AvaI C'yCGr_G
Cuts at: 0 115 160 1913 2280
Size: 115 45 1753 367
Fragments arranged by size:
1753 367 115 45
BamHI G'GATC_C
Cuts at: 0 200 1596 2280
Size: 200 1396 684
Fragments arranged by size:
1396 684 200
BanI G'GyrC_C
Cuts at: 0 897 1717 2280
Size: 897 820 563
Fragments arranged by size:
897 820 563
BanII G_rGCy'C
Cuts at: 0 76 127 167 178 185 626 657 666
Size: 76 51 40 11 7 441 31 9
Cuts at: 666 686 970 993 1027 1488 1498 2280
Size: 20 284 23 34 461 10 782
Fragments arranged by size:
782 461 441 284 76 51 40 34
31 23 20 11 10 9 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 827 917 932 1170 1303 2265 2280
Size: 827 90 15 238 133 962 15
Fragments arranged by size:
962 827 238 133 90 15 15
BbvCI CC'TCA_GC
Cuts at: 0 1258 1588 2280
Size: 1258 330 692
Fragments arranged by size:
1258 692 330
BglI GCCn_nnn'nGGC
Cuts at: 0 661 2280
Size: 661 1619
BglII A'GATC_T
Cuts at: 0 1230 2280
Size: 1230 1050
BlpI GC'TnA_GC
Cuts at: 0 1627 2280
Size: 1627 653
Bme1580I G_kGCm'C
Cuts at: 0 76 127 167 178 657 902 1012 1498
Size: 76 51 40 11 479 245 110 486
Cuts at: 1498 1896 2039 2280
Size: 398 143 241
Fragments arranged by size:
486 479 398 245 241 143 110 76
51 40 11
BmgBI CAC'GTC
Cuts at: 0 1301 1635 2280
Size: 1301 334 645
Fragments arranged by size:
1301 645 334
BmrI ACTGGGnnnn_n'
Cuts at: 0 95 1492 2280
Size: 95 1397 788
Fragments arranged by size:
1397 788 95
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 640 977 1660 1927 2157 2280
Size: 511 129 337 683 267 230 123
Fragments arranged by size:
683 511 337 267 230 129 123
Bpu10I CC'TnA_GC
Cuts at: 0 63 187 284 969 1258 1588 2280
Size: 63 124 97 685 289 330 692
Fragments arranged by size:
692 685 330 289 124 97 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1390 2280
Size: 1390 890
BsaI GGTCTCn'nnnn_
Cuts at: 0 105 2280
Size: 105 2175
BsaHI Gr'CG_yC
Cuts at: 0 1175 1655 2280
Size: 1175 480 625
Fragments arranged by size:
1175 625 480
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 33 736 1158 1888 2209 2280
Size: 22 11 703 422 730 321 71
Fragments arranged by size:
730 703 422 321 71 22 11
BseYI C'CCAG_C
Cuts at: 0 254 606 783 1133 1688 1738 2224 2280
Size: 254 352 177 350 555 50 486 56
Fragments arranged by size:
555 486 352 350 254 177 56 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 874 1749 2182 2280
Size: 190 684 875 433 98
Fragments arranged by size:
875 684 433 190 98
BsiEI CG_ry'CG
Cuts at: 0 20 29 2280
Size: 20 9 2251
Fragments arranged by size:
2251 20 9
BsiHKAI G_wGCw'C
Cuts at: 0 626 1012 1790 2039 2280
Size: 626 386 778 249 241
Fragments arranged by size:
778 626 386 249 241
BsmI GAATG_Cn'
Cuts at: 0 1064 1340 1452 2095 2280
Size: 1064 276 112 643 185
Fragments arranged by size:
1064 643 276 185 112
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2137 2280
Size: 2137 143
Bsp1286I G_dGCh'C
Cuts at: 0 76 127 167 178 185 626 657 666
Size: 76 51 40 11 7 441 31 9
Cuts at: 666 686 902 970 993 1012 1027 1488 1498
Size: 20 216 68 23 19 15 461 10
Cuts at: 1498 1790 1896 2039 2280
Size: 292 106 143 241
Fragments arranged by size:
461 441 292 241 216 143 106 76
68 51 40 31 23 20 19 15
11 10 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 121 1085 1427 2077 2280
Size: 121 964 342 650 203
Fragments arranged by size:
964 650 342 203 121
BsrBI CCG'CTC
Cuts at: 0 16 57 614 1853 2280
Size: 16 41 557 1239 427
Fragments arranged by size:
1239 557 427 41 16
BsrDI GCAATG_nn'
Cuts at: 0 2237 2280
Size: 2237 43
BsrFI r'CCGG_y
Cuts at: 0 2031 2280
Size: 2031 249
BssHII G'CGCG_C
Cuts at: 0 229 267 2280
Size: 229 38 2013
Fragments arranged by size:
2013 229 38
BssSI C'ACGA_G
Cuts at: 0 220 2191 2280
Size: 220 1971 89
Fragments arranged by size:
1971 220 89
BstBI TT'CG_AA
Cuts at: 0 236 2280
Size: 236 2044
BstEII G'GTnAC_C
Cuts at: 0 483 2280
Size: 483 1797
BstXI CCAn_nnnn'nTGG
Cuts at: 0 894 1806 2280
Size: 894 912 474
Fragments arranged by size:
912 894 474
BstYI r'GATC_y
Cuts at: 0 200 791 1230 1596 2280
Size: 200 591 439 366 684
Fragments arranged by size:
684 591 439 366 200
BtgI C'CryG_G
Cuts at: 0 23 167 473 1647 2042 2078 2280
Size: 23 144 306 1174 395 36 202
Fragments arranged by size:
1174 395 306 202 144 36 23
BtsI GCAGTG_nn'
Cuts at: 0 641 1896 2280
Size: 641 1255 384
Fragments arranged by size:
1255 641 384
DraIII CAC_nnn'GTG
Cuts at: 0 1839 2280
Size: 1839 441
EaeI y'GGCC_r
Cuts at: 0 17 1141 1436 2264 2280
Size: 17 1124 295 828 16
Fragments arranged by size:
1124 828 295 17 16
EagI C'GGCC_G
Cuts at: 0 17 2280
Size: 17 2263
EarI CTCTTCn'nnn_
Cuts at: 0 542 2280
Size: 542 1738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 93 164 363 2280
Size: 93 71 199 1917
Fragments arranged by size:
1917 199 93 71
EcoICRI GAG'CTC
Cuts at: 0 624 2280
Size: 624 1656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 394 424 511 640 977 1659 1660
Size: 283 111 30 87 129 337 682 1
Cuts at: 1660 1927 2157 2280
Size: 267 230 123
Fragments arranged by size:
682 337 283 267 230 129 123 111
87 30 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 424 547 1423 2266 2280
Size: 424 123 876 843 14
Fragments arranged by size:
876 843 424 123 14
EcoO109I rG'GnC_Cy
Cuts at: 0 73 653 654 673 1494 1665 1734 1794
Size: 73 580 1 19 821 171 69 60
Cuts at: 1794 2232 2280
Size: 438 48
Fragments arranged by size:
821 580 438 171 73 69 60 48
19 1
HaeII r_GCGC'y
Cuts at: 0 768 1456 1695 2255 2280
Size: 768 688 239 560 25
Fragments arranged by size:
768 688 560 239 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 568 600 2280
Size: 568 32 1680
Fragments arranged by size:
1680 568 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 1552 1954 2280
Size: 558 994 402 326
Fragments arranged by size:
994 558 402 326
MscI TGG'CCA
Cuts at: 0 2266 2280
Size: 2266 14
MslI CAynn'nnrTG
Cuts at: 0 1804 2280
Size: 1804 476
MspA1I CmG'CkG
Cuts at: 0 25 254 1262 1925 2280
Size: 25 229 1008 663 355
Fragments arranged by size:
1008 663 355 229 25
NcoI C'CATG_G
Cuts at: 0 2042 2280
Size: 2042 238
NotI GC'GGCC_GC
Cuts at: 0 17 2280
Size: 17 2263
NspI r_CATG'y
Cuts at: 0 582 1477 2280
Size: 582 895 803
Fragments arranged by size:
895 803 582
PasI CC'CwG_GG
Cuts at: 0 659 2280
Size: 659 1621
PflMI CCAn_nnn'nTGG
Cuts at: 0 1035 1638 1695 1839 2280
Size: 1035 603 57 144 441
Fragments arranged by size:
1035 603 441 144 57
PfoI T'CCnGG_A
Cuts at: 0 1637 2009 2280
Size: 1637 372 271
Fragments arranged by size:
1637 372 271
PpuMI rG'GwC_Cy
Cuts at: 0 1734 1794 2280
Size: 1734 60 486
Fragments arranged by size:
1734 486 60
PspOMI G'GGCC_C
Cuts at: 0 72 123 163 174 653 1494 2280
Size: 72 51 40 11 479 841 786
Fragments arranged by size:
841 786 479 72 51 40 11
PstI C_TGCA'G
Cuts at: 0 681 806 1424 2280
Size: 681 125 618 856
Fragments arranged by size:
856 681 618 125
PvuII CAG'CTG
Cuts at: 0 1262 2280
Size: 1262 1018
SacI G_AGCT'C
Cuts at: 0 626 2280
Size: 626 1654
SacII CC_GC'GG
Cuts at: 0 26 2280
Size: 26 2254
SanDI GG'GwC_CC
Cuts at: 0 1734 1794 2280
Size: 1734 60 486
Fragments arranged by size:
1734 486 60
SbfI CC_TGCA'GG
Cuts at: 0 681 2280
Size: 681 1599
SexAI A'CCwGG_T
Cuts at: 0 338 2280
Size: 338 1942
SfcI C'TryA_G
Cuts at: 0 677 802 1420 1539 2280
Size: 677 125 618 119 741
Fragments arranged by size:
741 677 618 125 119
SmaI CCC'GGG
Cuts at: 0 117 162 1915 2280
Size: 117 45 1753 365
Fragments arranged by size:
1753 365 117 45
SmlI C'TyrA_G
Cuts at: 0 1405 2280
Size: 1405 875
StyI C'CwwG_G
Cuts at: 0 887 1059 2042 2267 2280
Size: 887 172 983 225 13
Fragments arranged by size:
983 887 225 172 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 471 2280
Size: 471 1809
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1645 1677 2280
Size: 1645 32 603
Fragments arranged by size:
1645 603 32
Tth111I GACn'n_nGTC
Cuts at: 0 1864 2062 2280
Size: 1864 198 218
Fragments arranged by size:
1864 218 198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 2280
Size: 471 1809
XmaI C'CCGG_G
Cuts at: 0 115 160 1913 2280
Size: 115 45 1753 367
Fragments arranged by size:
1753 367 115 45
ZraI GAC'GTC
Cuts at: 0 1176 2280
Size: 1176 1104
Enzymes that do cut:
AarI AatII AcuI AfeI AhdI AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstBI BstEII BstXI BstYI BtgI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I MmeI MscI MslI MspA1I NcoI NotI
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SacII SanDI SbfI SexAI SfcI SmaI SmlI
StyI TaqII TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BfrBI BmtI BplI BsaAI BsaBI BsaWI BsaXI
BsiWI BspEI BspHI BsrGI BstAPI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SapI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XhoI XmnI