Restriction map for
pFN21AB3487
(Circular) (Six-base) MAPSORT of: pFN21AB3487.seq Check: 4162 from: 1 to: 6697
pFN21AB3487 6697 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3189 4507 278
Size: 53 83 186 936 1653 1318 2468
Fragments arranged by size:
2468 1653 1318 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3096 4040 1937
Size: 1159 944 4594
Fragments arranged by size:
4594 1159 944
Acc65I G'GTAC_C
Cuts at: 4018 4018
Size: 6697
AclI AA'CG_TT
Cuts at: 1563 4824 5197 1563
Size: 3261 373 3063
Fragments arranged by size:
3261 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2514 2779 3671 4760 6144 6412 1632
Size: 882 265 892 1089 1384 268 1917
Fragments arranged by size:
1917 1384 1089 892 882 268 265
AfeI AGC'GCT
Cuts at: 2041 2041
Size: 6697
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6500
AflIII A'CryG_T
Cuts at: 1203 5596 6573 1203
Size: 4393 977 1327
Fragments arranged by size:
4393 1327 977
AgeI A'CCGG_T
Cuts at: 5528 5528
Size: 6697
AhdI GACnn_n'nnGTC
Cuts at: 3016 5426 3016
Size: 2410 4287
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6697
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3585 1184
Size: 2401 4296
AlwNI CAG_nnn'CTG
Cuts at: 3268 3715 4440 6012 3268
Size: 447 725 1572 3953
Fragments arranged by size:
3953 1572 725 447
ApaLI G'TGCA_C
Cuts at: 4754 5910 4754
Size: 1156 5541
ApoI r'AATT_y
Cuts at: 1493 2186 4006 4232 4384 6493 1493
Size: 693 1820 226 152 2109 1697
Fragments arranged by size:
2109 1820 1697 693 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5251 160
Size: 872 4219 1606
Fragments arranged by size:
4219 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
AvaI C'yCGr_G
Cuts at: 1870 1943 2094 3359 4022 1870
Size: 73 151 1265 663 4545
Fragments arranged by size:
4545 1265 663 151 73
BamHI G'GATC_C
Cuts at: 4027 4394 4027
Size: 367 6330
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 3347 4018 5473 618
Size: 325 217 627 259 1301 671 1455 1842
Fragments arranged by size:
1842 1455 1301 671 627 325 259 217
BanII G_rGCy'C
Cuts at: 1391 2393 4016 1391
Size: 1002 1623 4072
Fragments arranged by size:
4072 1623 1002
BbeI G_GCGC'C
Cuts at: 2050 2050
Size: 6697
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3659 3795 928
Size: 971 1760 136 3830
Fragments arranged by size:
3830 1760 971 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2546 2580 4888 4922 1903
Size: 34 609 34 2308 34 3678
Fragments arranged by size:
3678 2308 609 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2178 4589 5799 2178
Size: 2411 1210 3076
Fragments arranged by size:
3076 2411 1210
BclI T'GATC_A
Cuts at: 1548 2841 1548
Size: 1293 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3147 5308 136
Size: 107 122 71 631 605 1475 2161 1525
Fragments arranged by size:
2161 1525 1475 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6692 6692
Size: 6697
BlpI GC'TnA_GC
Cuts at: 2036 4114 2036
Size: 2078 4619
Bme1580I G_kGCm'C
Cuts at: 1790 3303 3409 4758 5914 1790
Size: 1513 106 1349 1156 2573
Fragments arranged by size:
2573 1513 1349 1156 106
BmgBI CAC'GTC
Cuts at: 1318 2805 3094 1318
Size: 1487 289 4921
Fragments arranged by size:
4921 1487 289
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3915 4428 5386
Size: 416 53 83 399 24 2498 513 958
Cuts at: 5386 442
Size: 1753
Fragments arranged by size:
2498 1753 958 513 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6697
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 2972
Size: 6697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2380 3071 3105 3456 3722 3889 5357
Size: 993 148 691 34 351 266 167 1468
Cuts at: 5357 1239
Size: 2579
Fragments arranged by size:
2579 1468 993 691 351 266 167 148
34
Bpu10I CC'TnA_GC
Cuts at: 3410 3410
Size: 6697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2522 4822 5687 5985 2522
Size: 2300 865 298 3234
Fragments arranged by size:
3234 2300 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2631 3630 3689 5360 882
Size: 617 1132 999 59 1671 2219
Fragments arranged by size:
2219 1671 1132 999 617 59
BsaAI yAC'GTr
Cuts at: 493 1204 3277 4488 493
Size: 711 2073 1211 2702
Fragments arranged by size:
2702 2073 1211 711
BsaBI GATnn'nnATC
Cuts at: 6691 6691
Size: 6697
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2047 3017 3186 4504
Size: 53 83 186 936 514 970 169 1318
Cuts at: 4504 4886 5521 275
Size: 382 635 1451
Fragments arranged by size:
1451 1318 970 936 635 514 382 186
169 83 53
BsaWI w'CCGG_w
Cuts at: 5130 5528 5802 5949 6360 5130
Size: 398 274 147 411 5467
Fragments arranged by size:
5467 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3429 3459 3806 3836 1178
Size: 30 165 30 2026 30 347 30 4039
Fragments arranged by size:
4039 2026 347 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 3875 1186
Size: 1177 1512 4008
Fragments arranged by size:
4008 1512 1177
BseYI C'CCAG_C
Cuts at: 2401 3467 5900 6396 2401
Size: 1066 2433 496 2702
Fragments arranged by size:
2702 2433 1066 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2785 3092 1163
Size: 573 1049 307 4768
Fragments arranged by size:
4768 1049 573 307
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3041 3664 4147 4908 5936 1540
Size: 275 186 1040 623 483 761 1028 2301
Fragments arranged by size:
2301 1040 1028 761 623 483 275 186
BsiHKAI G_wGCw'C
Cuts at: 3867 4758 4843 5914 3867
Size: 891 85 1071 4650
Fragments arranged by size:
4650 1071 891 85
BsmI GAATG_Cn'
Cuts at: 2952 3240 4208 4301 2952
Size: 288 968 93 5348
Fragments arranged by size:
5348 968 288 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2393 3303 3409 3867 4016 4758 4843
Size: 399 603 910 106 458 149 742 85
Cuts at: 4843 5914 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 910 742 603 458 399 149
106 85
BspEI T'CCGG_A
Cuts at: 6360 6360
Size: 6697
BspHI T'CATG_A
Cuts at: 2182 3483 4586 6316 2182
Size: 1301 1103 1730 2563
Fragments arranged by size:
2563 1730 1301 1103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3108 3253 4052 844
Size: 2264 145 799 3489
Fragments arranged by size:
3489 2264 799 145
BsrBI CCG'CTC
Cuts at: 1394 1823 4584 1394
Size: 429 2761 3507
Fragments arranged by size:
3507 2761 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5192 5366 65
Size: 1183 3944 174 1396
Fragments arranged by size:
3944 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5341 5528 1700
Size: 110 3531 187 2869
Fragments arranged by size:
3531 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6697
BssHII G'CGCG_C
Cuts at: 1925 3774 1925
Size: 1849 4848
BssSI C'ACGA_G
Cuts at: 4757 5769 4757
Size: 1012 5685
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6697
BstBI TT'CG_AA
Cuts at: 5501 5501
Size: 6697
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6697
BstYI r'GATC_y
Cuts at: 1972 2671 3054 4027 4394 4779 4796 6248 6334
Size: 699 383 973 367 385 17 1452 86
Cuts at: 6334 6346 6692 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 973 699 385 383 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3269 3269
Size: 6697
BtgI C'CryG_G
Cuts at: 513 1065 2451 2698 513
Size: 552 1386 247 4512
Fragments arranged by size:
4512 1386 552 247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2692 2723 487
Size: 683 56 1466 31 4461
Fragments arranged by size:
4461 1466 683 56 31
BtsI GCAGTG_nn'
Cuts at: 2202 4213 5007 5027 2202
Size: 2011 794 20 3872
Fragments arranged by size:
3872 2011 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6662
DraI TTT'AAA
Cuts at: 4001 4001
Size: 6697
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3386 5704 809
Size: 507 2070 2318 1802
Fragments arranged by size:
2318 2070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2069 2593
Size: 54 1425 50 275 18 228 11 524
Cuts at: 2593 2835 3132 3283 4144 5033 6662 8
Size: 242 297 151 861 889 1629 43
Fragments arranged by size:
1629 1425 889 861 524 297 275 242
228 151 54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 4144 1537
Size: 275 2332 4090
Fragments arranged by size:
4090 2332 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3061 3414 4627 1353
Size: 1708 353 1213 3423
Fragments arranged by size:
3423 1708 1213 353
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5284 5658 5804 387
Size: 1064 138 3695 374 146 1280
Fragments arranged by size:
3695 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2380 2514 2779 3071 3105 3456
Size: 393 600 148 134 265 292 34 351
Cuts at: 3456 3671 3722 3889 4760 5357 6144 6412 1239
Size: 215 51 167 871 597 787 268 1524
Fragments arranged by size:
1524 871 787 600 597 393 351 292
268 265 215 167 148 134 51 34
EcoNI CCTnn'n_nnAGG
Cuts at: 2246 2246
Size: 6697
EcoO109I rG'GnC_Cy
Cuts at: 2020 2440 2869 3010 3265 2020
Size: 420 429 141 255 5452
Fragments arranged by size:
5452 429 420 255 141
EcoRI G'AATT_C
Cuts at: 4006 4384 4006
Size: 378 6319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3871 3871
Size: 6697
FspI TGC'GCA
Cuts at: 5203 5203
Size: 6697
HaeII r_GCGC'y
Cuts at: 1678 2043 2050 2057 2211 5844 1678
Size: 365 7 7 154 3633 2531
Fragments arranged by size:
3633 2531 365 154 7 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2520 2552 2714 2746 3002
Size: 32 135 32 948 32 162 32 256
Cuts at: 3002 3017 3034 3049 3429 3461 3921 3953 5338
Size: 15 17 15 380 32 460 32 1385
Cuts at: 5338 5370 5412 5444 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1385 948 460 380 256 162 135
42 32 32 32 32 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4041 4287 669
Size: 988 281 2103 246 3079
Fragments arranged by size:
3079 2103 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6697
HpaI GTT'AAC
Cuts at: 4287 4287
Size: 6697
KasI G'GCGC_C
Cuts at: 2046 2046
Size: 6697
KpnI G_GTAC'C
Cuts at: 4022 4022
Size: 6697
MfeI C'AATT_G
Cuts at: 4296 4296
Size: 6697
MluI A'CGCG_T
Cuts at: 6573 6573
Size: 6697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2418 5811 5995 1305
Size: 1113 3393 184 2007
Fragments arranged by size:
3393 2007 1113 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 2071 2595 3134 3285
Size: 54 1425 343 228 11 524 539 151
Cuts at: 3285 10
Size: 3422
Fragments arranged by size:
3422 1425 539 524 343 228 151 54
11
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 3279 4655 5014 5173 518
Size: 648 1671 442 1376 359 159 2042
Fragments arranged by size:
2042 1671 1376 648 442 359 159
MspA1I CmG'CkG
Cuts at: 1606 2799 3106 3130 4113 4790 5938 6183 1606
Size: 1193 307 24 983 677 1148 245 2120
Fragments arranged by size:
2120 1193 1148 983 677 307 245 24
NarI GG'CG_CC
Cuts at: 2047 2047
Size: 6697
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6145
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6697
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6697
NotI GC'GGCC_GC
Cuts at: 4144 4144
Size: 6697
NruI TCG'CGA
Cuts at: 3005 3005
Size: 6697
NspI r_CATG'y
Cuts at: 1132 1229 2271 2479 3405 3550 3782 4055 5600
Size: 97 1042 208 926 145 232 273 1545
Cuts at: 5600 1132
Size: 2229
Fragments arranged by size:
2229 1545 1042 926 273 232 208 145
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6697
PciI A'CATG_T
Cuts at: 5596 5596
Size: 6697
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2067 3467 1108
Size: 959 1400 4338
Fragments arranged by size:
4338 1400 959
PmeI GTTT'AAAC
Cuts at: 4001 4001
Size: 6697
PmlI CAC'GTG
Cuts at: 3277 3277
Size: 6697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3525 3557 4766 4798 6279 6311 3525
Size: 32 1209 32 1481 32 3911
Fragments arranged by size:
3911 1481 1209 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2869 3010 3265 2869
Size: 141 255 6301
Fragments arranged by size:
6301 255 141
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6697
PsiI TTA'TAA
Cuts at: 3228 4267 3228
Size: 1039 5658
PstI C_TGCA'G
Cuts at: 830 3105 3711 3735 4049 830
Size: 2275 606 24 314 3478
Fragments arranged by size:
3478 2275 606 314 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6697
PvuII CAG'CTG
Cuts at: 2799 3106 3130 2799
Size: 307 24 6366
Fragments arranged by size:
6366 307 24
SalI G'TCGA_C
Cuts at: 1936 4039 1936
Size: 2103 4594
SbfI CC_TGCA'GG
Cuts at: 4049 4049
Size: 6697
ScaI AGT'ACT
Cuts at: 4945 6657 4945
Size: 1712 4985
SexAI A'CCwGG_T
Cuts at: 2291 3381 3687 2291
Size: 1090 306 5301
Fragments arranged by size:
5301 1090 306
SfcI C'TryA_G
Cuts at: 826 1045 3101 3707 3731 4045 5180 5861 6052
Size: 219 2056 606 24 314 1135 681 191
Cuts at: 6052 826
Size: 1471
Fragments arranged by size:
2056 1471 1135 681 606 314 219 191
24
SfoI GGC'GCC
Cuts at: 2048 2048
Size: 6697
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6697
SmaI CCC'GGG
Cuts at: 1872 4024 1872
Size: 2152 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2537 4801 5702 5964 820
Size: 197 926 594 2264 901 262 1553
Fragments arranged by size:
2264 1553 926 901 594 262 197
SnaBI TAC'GTA
Cuts at: 493 4488 493
Size: 3995 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6697
SphI G_CATG'C
Cuts at: 1132 2479 3550 3782 4055 1132
Size: 1347 1071 232 273 3774
Fragments arranged by size:
3774 1347 1071 273 232
SspI AAT'ATT
Cuts at: 5 52 4621 5
Size: 47 4569 2081
Fragments arranged by size:
4569 2081 47
StuI AGG'CCT
Cuts at: 1517 2856 3433 1517
Size: 1339 577 4781
Fragments arranged by size:
4781 1339 577
StyI C'CwwG_G
Cuts at: 513 1065 2061 2667 4136 513
Size: 552 996 606 1469 3074
Fragments arranged by size:
3074 1469 996 606 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3027 4724 4741 4894 5079 5524 325
Size: 1255 1447 1697 17 153 185 445 1498
Fragments arranged by size:
1697 1498 1447 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4943 6655 96
Size: 274 80 33 51 1443 2966 1712 138
Fragments arranged by size:
2966 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3869 3901 6657 6689 3869
Size: 32 2756 32 3877
Fragments arranged by size:
3877 2756 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6697
XbaI T'CTAG_A
Cuts at: 3020 4033 3020
Size: 1013 5684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2650 2819 2650
Size: 169 6528
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6697
XmaI C'CCGG_G
Cuts at: 1870 4022 1870
Size: 2152 4545
XmnI GAAnn'nnTTC
Cuts at: 3656 4826 3656
Size: 1170 5527
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3187 4505 276
Size: 53 83 186 936 1653 1318 2468
Fragments arranged by size:
2468 1653 1318 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NsiI PacI PfoI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SrfI
SwaI