(Circular) (Six-base) MAPSORT of: pFN21AB3314.seq Check: 7291 from: 1 to: 6832 pFN21AB3314 6832 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2523 3710 4642 278 Size: 53 83 186 936 987 1187 932 2468 Fragments arranged by size: 2468 1187 987 936 932 186 83 53 AccI GT'mk_AC Cuts at: 1937 4175 1937 Size: 2238 4594 Acc65I G'GTAC_C Cuts at: 3577 4153 3577 Size: 576 6256 AclI AA'CG_TT Cuts at: 1563 4959 5332 1563 Size: 3396 373 3063 Fragments arranged by size: 3396 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2157 3184 3693 4011 4895 6279 6547 1632 Size: 525 1027 509 318 884 1384 268 1917 Fragments arranged by size: 1917 1384 1027 884 525 509 318 268 AflII C'TTAA_G Cuts at: 820 1017 2186 820 Size: 197 1169 5466 Fragments arranged by size: 5466 1169 197 AflIII A'CryG_T Cuts at: 1203 2585 3944 5731 6708 1203 Size: 1382 1359 1787 977 1327 Fragments arranged by size: 1787 1382 1359 1327 977 AgeI A'CCGG_T Cuts at: 5663 5663 Size: 6832 AhdI GACnn_n'nnGTC Cuts at: 2284 2509 3259 5561 2284 Size: 225 750 2302 3555 Fragments arranged by size: 3555 2302 750 225 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6832 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6832 AlwNI CAG_nnn'CTG Cuts at: 2294 4575 6147 2294 Size: 2281 1572 2979 Fragments arranged by size: 2979 2281 1572 ApaLI G'TGCA_C Cuts at: 3545 4889 6045 3545 Size: 1344 1156 4332 Fragments arranged by size: 4332 1344 1156 ApoI r'AATT_y Cuts at: 1493 2033 2194 2561 3775 4141 4367 4519 6628 Size: 540 161 367 1214 366 226 152 2109 Cuts at: 6628 1493 Size: 1697 Fragments arranged by size: 2109 1697 1214 540 367 366 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 3362 5386 160 Size: 872 2330 2024 1606 Fragments arranged by size: 2330 2024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6832 AvaI C'yCGr_G Cuts at: 1870 1943 4157 1870 Size: 73 2214 4545 Fragments arranged by size: 4545 2214 73 BamHI G'GATC_C Cuts at: 4162 4529 4162 Size: 367 6465 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3577 4153 5608 618 Size: 325 217 627 1790 576 1455 1842 Fragments arranged by size: 1842 1790 1455 627 576 325 217 BanII G_rGCy'C Cuts at: 1391 2274 4151 1391 Size: 883 1877 4072 Fragments arranged by size: 4072 1877 883 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3068 928 Size: 971 1169 4692 Fragments arranged by size: 4692 1169 971 BbvCI CC'TCA_GC Cuts at: 2105 2105 Size: 6832 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3536 3570 5023 5057 1903 Size: 34 1599 34 1453 34 3678 Fragments arranged by size: 3678 1599 1453 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2251 2654 4724 5934 2251 Size: 403 2070 1210 3149 Fragments arranged by size: 3149 2070 1210 403 BclI T'GATC_A Cuts at: 1548 2598 1548 Size: 1050 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5443 136 Size: 107 122 71 631 605 3771 1525 Fragments arranged by size: 3771 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2182 2775 2885 6827 2182 Size: 593 110 3942 2187 Fragments arranged by size: 3942 2187 593 110 BlpI GC'TnA_GC Cuts at: 4249 4249 Size: 6832 Bme1580I G_kGCm'C Cuts at: 1790 3481 3549 4893 6049 1790 Size: 1691 68 1344 1156 2573 Fragments arranged by size: 2573 1691 1344 1156 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6832 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4563 5521 442 Size: 416 53 83 399 24 3146 958 1753 Fragments arranged by size: 3146 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6832 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2062 2064 2062 Size: 2 6830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2792 2915 3001 3209 3732 5492 1239 Size: 1553 123 86 208 523 1760 2579 Fragments arranged by size: 2579 1760 1553 523 208 123 86 Bpu10I CC'TnA_GC Cuts at: 2105 2357 2105 Size: 252 6580 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4957 5822 6120 4957 Size: 865 298 5669 Fragments arranged by size: 5669 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5495 882 Size: 617 3996 2219 Fragments arranged by size: 3996 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3945 4623 493 Size: 711 2741 678 2702 Fragments arranged by size: 2741 2702 711 678 BsaBI GATnn'nnATC Cuts at: 4050 6826 4050 Size: 2776 4056 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2520 3707 4639 5021 Size: 53 83 186 936 987 1187 932 382 Cuts at: 5021 5656 275 Size: 635 1451 Fragments arranged by size: 1451 1187 987 936 932 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2065 2822 5265 5663 5937 6084 6495 2065 Size: 757 2443 398 274 147 411 2402 Fragments arranged by size: 2443 2402 757 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2417 2447 1178 Size: 30 165 30 1014 30 5563 Fragments arranged by size: 5563 1014 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2377 2723 3206 3234 3602 3650 1186 Size: 1191 346 483 28 368 48 4368 Fragments arranged by size: 4368 1191 483 368 346 48 28 BseYI C'CCAG_C Cuts at: 2411 3226 6035 6531 2411 Size: 815 2809 496 2712 Fragments arranged by size: 2809 2712 815 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2426 2610 2934 1163 Size: 573 690 184 324 5061 Fragments arranged by size: 5061 690 573 324 184 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4282 5043 6071 1540 Size: 275 186 2281 761 1028 2301 Fragments arranged by size: 2301 2281 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2274 3549 4893 4978 6049 2274 Size: 1275 1344 85 1071 3057 Fragments arranged by size: 3057 1344 1275 1071 85 BsmI GAATG_Cn' Cuts at: 4343 4436 4343 Size: 93 6739 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2274 3481 3549 4151 4893 4978 6049 Size: 399 484 1207 68 602 742 85 1071 Cuts at: 6049 1391 Size: 2174 Fragments arranged by size: 2174 1207 1071 742 602 484 399 85 68 BspEI T'CCGG_A Cuts at: 6495 6495 Size: 6832 BspHI T'CATG_A Cuts at: 4721 6451 4721 Size: 1730 5102 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2067 4187 844 Size: 1223 2120 3489 Fragments arranged by size: 3489 2120 1223 BsrBI CCG'CTC Cuts at: 1394 1823 4719 1394 Size: 429 2896 3507 Fragments arranged by size: 3507 2896 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3063 5327 5501 65 Size: 1183 1815 2264 174 1396 Fragments arranged by size: 2264 1815 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5476 5663 1700 Size: 110 3666 187 2869 Fragments arranged by size: 3666 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6832 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6832 BssSI C'ACGA_G Cuts at: 3765 4892 5904 3765 Size: 1127 1012 4693 Fragments arranged by size: 4693 1127 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6832 BstBI TT'CG_AA Cuts at: 2992 5636 2992 Size: 2644 4188 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6832 BstYI r'GATC_y Cuts at: 1972 2182 2613 2775 2885 4162 4529 4914 4931 Size: 210 431 162 110 1277 367 385 17 Cuts at: 4931 6383 6469 6481 6827 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1277 431 385 367 346 210 162 110 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2098 513 Size: 552 939 94 5247 Fragments arranged by size: 5247 939 552 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3152 487 Size: 683 56 1926 4167 Fragments arranged by size: 4167 1926 683 56 BtsI GCAGTG_nn' Cuts at: 2409 4348 5142 5162 2409 Size: 1939 794 20 4079 Fragments arranged by size: 4079 1939 794 20 ClaI AT'CG_AT Cuts at: 1554 3366 1554 Size: 1812 5020 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2200 2235 3253 3288 3748 3783 529 Size: 35 1636 35 1018 35 460 35 3578 Fragments arranged by size: 3578 1636 1018 460 35 35 35 35 DraI TTT'AAA Cuts at: 3523 3991 4136 3523 Size: 468 145 6219 Fragments arranged by size: 6219 468 145 DraIII CAC_nnn'GTG Cuts at: 2404 2404 Size: 6832 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5839 809 Size: 507 4523 1802 Fragments arranged by size: 4523 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3018 3627 4279 Size: 54 1425 50 275 18 1188 609 652 Cuts at: 4279 5168 6797 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1188 889 652 609 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4279 1537 Size: 275 2467 4090 Fragments arranged by size: 4090 2467 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4762 1353 Size: 3409 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5419 5793 5939 387 Size: 1064 138 3830 374 146 1280 Fragments arranged by size: 3830 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2272 2272 Size: 6832 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2792 2915 3001 3184 3209 3693 Size: 393 525 635 123 86 183 25 484 Cuts at: 3693 3732 4011 4895 5492 6279 6547 1239 Size: 39 279 884 597 787 268 1524 Fragments arranged by size: 1524 884 787 635 597 525 484 393 279 268 183 123 86 39 25 EcoNI CCTnn'n_nnAGG Cuts at: 2534 2969 2534 Size: 435 6397 EcoO109I rG'GnC_Cy Cuts at: 2647 3157 2647 Size: 510 6322 EcoRI G'AATT_C Cuts at: 4141 4519 4141 Size: 378 6454 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2530 2934 2530 Size: 404 6428 FspI TGC'GCA Cuts at: 5338 5338 Size: 6832 HaeII r_GCGC'y Cuts at: 1678 5979 1678 Size: 4301 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 2495 2527 3245 Size: 32 135 32 698 32 193 32 718 Cuts at: 3245 3277 3340 3359 3372 3391 3435 3467 3779 Size: 32 63 19 13 19 44 32 312 Cuts at: 3779 3811 4060 4092 5473 5505 5547 5579 1373 Size: 32 249 32 1381 32 42 32 2626 Fragments arranged by size: 2626 1381 718 698 312 249 193 135 63 44 42 32 32 32 32 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 3508 4176 4422 669 Size: 988 281 1570 668 246 3079 Fragments arranged by size: 3079 1570 988 668 281 246 HindIII A'AGCT_T Cuts at: 748 2169 748 Size: 1421 5411 HpaI GTT'AAC Cuts at: 4422 4422 Size: 6832 KpnI G_GTAC'C Cuts at: 3581 4157 3581 Size: 576 6256 MfeI C'AATT_G Cuts at: 4431 4431 Size: 6832 MluI A'CGCG_T Cuts at: 6708 6708 Size: 6832 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2437 3067 5946 6130 1305 Size: 1132 630 2879 184 2007 Fragments arranged by size: 2879 2007 1132 630 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3020 10 Size: 54 1425 343 1188 3822 Fragments arranged by size: 3822 1425 1188 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2910 3026 4790 5149 5308 518 Size: 648 1744 116 1764 359 159 2042 Fragments arranged by size: 2042 1764 1744 648 359 159 116 MspA1I CmG'CkG Cuts at: 1606 2100 2477 2798 4248 4925 6073 6318 1606 Size: 494 377 321 1450 677 1148 245 2120 Fragments arranged by size: 2120 1450 1148 677 494 377 321 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5341 Fragments arranged by size: 5341 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6832 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6832 NotI GC'GGCC_GC Cuts at: 4279 4279 Size: 6832 NspI r_CATG'y Cuts at: 1132 1229 4190 5735 1132 Size: 97 2961 1545 2229 Fragments arranged by size: 2961 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6832 PciI A'CATG_T Cuts at: 5731 5731 Size: 6832 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6832 PfoI T'CCnGG_A Cuts at: 2253 3186 2253 Size: 933 5899 PmeI GTTT'AAAC Cuts at: 4136 4136 Size: 6832 PmlI CAC'GTG Cuts at: 3945 3945 Size: 6832 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4901 4933 6414 6446 4901 Size: 32 1481 32 5287 Fragments arranged by size: 5287 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3157 3157 Size: 6832 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6832 PsiI TTA'TAA Cuts at: 4402 4402 Size: 6832 PstI C_TGCA'G Cuts at: 830 3075 4184 830 Size: 2245 1109 3478 Fragments arranged by size: 3478 2245 1109 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6832 PvuII CAG'CTG Cuts at: 2477 2798 2477 Size: 321 6511 SacI G_AGCT'C Cuts at: 2274 2274 Size: 6832 SacII CC_GC'GG Cuts at: 2101 2101 Size: 6832 SalI G'TCGA_C Cuts at: 1936 4174 1936 Size: 2238 4594 SbfI CC_TGCA'GG Cuts at: 4184 4184 Size: 6832 ScaI AGT'ACT Cuts at: 5080 6792 5080 Size: 1712 5120 SfcI C'TryA_G Cuts at: 826 1045 3071 4180 5315 5996 6187 826 Size: 219 2026 1109 1135 681 191 1471 Fragments arranged by size: 2026 1471 1135 1109 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6832 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6832 SmaI CCC'GGG Cuts at: 1872 4159 1872 Size: 2287 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 4936 5837 6099 820 Size: 197 926 243 2750 901 262 1553 Fragments arranged by size: 2750 1553 926 901 262 243 197 SnaBI TAC'GTA Cuts at: 493 4623 493 Size: 4130 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6832 SphI G_CATG'C Cuts at: 1132 4190 1132 Size: 3058 3774 SspI AAT'ATT Cuts at: 5 52 2953 4756 5 Size: 47 2901 1803 2081 Fragments arranged by size: 2901 2081 1803 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6832 StyI C'CwwG_G Cuts at: 513 1065 2004 2019 2997 3573 4271 513 Size: 552 939 15 978 576 698 3074 Fragments arranged by size: 3074 978 939 698 576 552 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2844 3270 3356 4859 4876 5029 5214 Size: 1255 1264 426 86 1503 17 153 185 Cuts at: 5214 5659 325 Size: 445 1498 Fragments arranged by size: 1503 1498 1264 1255 445 426 185 153 86 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2890 5078 6790 Size: 274 80 33 51 1443 913 2188 1712 Cuts at: 6790 96 Size: 138 Fragments arranged by size: 2188 1712 1443 913 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6792 6824 6792 Size: 32 6800 Tth111I GACn'n_nGTC Cuts at: 1316 2854 3178 1316 Size: 1538 324 4970 Fragments arranged by size: 4970 1538 324 XbaI T'CTAG_A Cuts at: 4168 4168 Size: 6832 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3150 3150 Size: 6832 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6832 XmaI C'CCGG_G Cuts at: 1870 4157 1870 Size: 2287 4545 XmnI GAAnn'nnTTC Cuts at: 3877 4961 3877 Size: 1084 5748 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2521 3708 4640 276 Size: 53 83 186 936 987 1187 932 2468 Fragments arranged by size: 2468 1187 987 936 932 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI