Restriction map for
pFN21AE0100
(Linear) (Six-base) MAPSORT of: KIEE0100.seq Check: 8287 from: 1 to: 2496
KIEE0100.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 713 2202 2496
Size: 713 1489 294
Fragments arranged by size:
1489 713 294
AccI GT'mk_AC
Cuts at: 0 891 1375 2496
Size: 891 484 1121
Fragments arranged by size:
1121 891 484
AclI AA'CG_TT
Cuts at: 0 1559 2496
Size: 1559 937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2445 2496
Size: 2445 51
AfeI AGC'GCT
Cuts at: 0 2232 2496
Size: 2232 264
AflIII A'CryG_T
Cuts at: 0 2345 2496
Size: 2345 151
AleI CACnn'nnGTG
Cuts at: 0 913 2496
Size: 913 1583
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1549 1581 2253 2285 2496
Size: 1549 32 672 32 211
Fragments arranged by size:
1549 672 211 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 175 2496
Size: 175 2321
ApaI G_GGCC'C
Cuts at: 0 65 2496
Size: 65 2431
ApoI r'AATT_y
Cuts at: 0 694 1042 1483 1972 2039 2452 2496
Size: 694 348 441 489 67 413 44
Fragments arranged by size:
694 489 441 413 348 67 44
AseI AT'TA_AT
Cuts at: 0 1357 2485 2496
Size: 1357 1128 11
Fragments arranged by size:
1357 1128 11
AvaI C'yCGr_G
Cuts at: 0 64 144 1328 2496
Size: 64 80 1184 1168
Fragments arranged by size:
1184 1168 80 64
BamHI G'GATC_C
Cuts at: 0 1958 2496
Size: 1958 538
BanI G'GyrC_C
Cuts at: 0 148 783 2496
Size: 148 635 1713
Fragments arranged by size:
1713 635 148
BanII G_rGCy'C
Cuts at: 0 65 118 145 2165 2496
Size: 65 53 27 2020 331
Fragments arranged by size:
2020 331 65 53 27
BbeI G_GCGC'C
Cuts at: 0 152 2496
Size: 152 2344
BbsI GAAGACnn'nnnn_
Cuts at: 0 804 936 1707 2496
Size: 804 132 771 789
Fragments arranged by size:
804 789 771 132
BciVI GTATCCnnnnn_n'
Cuts at: 0 823 1837 2496
Size: 823 1014 659
Fragments arranged by size:
1014 823 659
BglI GCCn_nnn'nGGC
Cuts at: 0 301 1136 2496
Size: 301 835 1360
Fragments arranged by size:
1360 835 301
Bme1580I G_kGCm'C
Cuts at: 0 65 187 2496
Size: 65 122 2309
Fragments arranged by size:
2309 122 65
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 469 799 1330 2191 2255 2467 2496
Size: 292 177 330 531 861 64 212 29
Fragments arranged by size:
861 531 330 292 212 177 64 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 600 1228 1282 1679 2179 2314 2496
Size: 600 628 54 397 500 135 182
Fragments arranged by size:
628 600 500 397 182 135 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 1405 2162 2496
Size: 1405 757 334
Fragments arranged by size:
1405 757 334
BsaAI yAC'GTr
Cuts at: 0 1491 2346 2496
Size: 1491 855 150
Fragments arranged by size:
1491 855 150
BsaHI Gr'CG_yC
Cuts at: 0 149 2496
Size: 149 2347
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 442 472 1218 1248 1549 1579 2253 2283
Size: 442 30 746 30 301 30 674 30
Cuts at: 2283 2496
Size: 213
Fragments arranged by size:
746 674 442 301 213 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 322 1327 2496
Size: 322 1005 1169
Fragments arranged by size:
1169 1005 322
BseYI C'CCAG_C
Cuts at: 0 1171 2496
Size: 1171 1325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 2496
Size: 706 1790
BsiEI CG_ry'CG
Cuts at: 0 191 2496
Size: 191 2305
BsmI GAATG_Cn'
Cuts at: 0 1589 2105 2496
Size: 1589 516 391
Fragments arranged by size:
1589 516 391
Bsp1286I G_dGCh'C
Cuts at: 0 65 118 145 187 2165 2496
Size: 65 53 27 42 1978 331
Fragments arranged by size:
1978 331 65 53 42 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 380 713 2202 2496
Size: 380 333 1489 294
Fragments arranged by size:
1489 380 333 294
BsrBI CCG'CTC
Cuts at: 0 253 2496
Size: 253 2243
BsrDI GCAATG_nn'
Cuts at: 0 928 1403 2496
Size: 928 475 1093
Fragments arranged by size:
1093 928 475
BstAPI GCAn_nnn'nTGC
Cuts at: 0 175 2496
Size: 175 2321
BstBI TT'CG_AA
Cuts at: 0 2043 2496
Size: 2043 453
BstXI CCAn_nnnn'nTGG
Cuts at: 0 270 1935 2445 2496
Size: 270 1665 510 51
Fragments arranged by size:
1665 510 270 51
BstYI r'GATC_y
Cuts at: 0 1529 1958 2304 2496
Size: 1529 429 346 192
Fragments arranged by size:
1529 429 346 192
BtgI C'CryG_G
Cuts at: 0 256 1954 2300 2496
Size: 256 1698 346 196
Fragments arranged by size:
1698 346 256 196
BtsI GCAGTG_nn'
Cuts at: 0 176 2496
Size: 176 2320
DraI TTT'AAA
Cuts at: 0 2073 2496
Size: 2073 423
DraIII CAC_nnn'GTG
Cuts at: 0 851 2496
Size: 851 1645
EaeI y'GGCC_r
Cuts at: 0 188 1436 1653 2496
Size: 188 1248 217 843
Fragments arranged by size:
1248 843 217 188
EagI C'GGCC_G
Cuts at: 0 188 2496
Size: 188 2308
EarI CTCTTCn'nnn_
Cuts at: 0 1122 1257 1457 2212 2251 2496
Size: 1122 135 200 755 39 245
Fragments arranged by size:
1122 755 245 200 135 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 86 282 2496
Size: 86 196 2214
Fragments arranged by size:
2214 196 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 469 799 1330 2191 2255 2445 2467
Size: 292 177 330 531 861 64 190 22
Cuts at: 2467 2496
Size: 29
Fragments arranged by size:
861 531 330 292 190 177 64 29
22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2209 2496
Size: 2209 287
HaeII r_GCGC'y
Cuts at: 0 73 94 152 2234 2496
Size: 73 21 58 2082 262
Fragments arranged by size:
2082 262 73 58 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 442 474 772 804 1093 1125 1218 1250
Size: 442 32 298 32 289 32 93 32
Cuts at: 1250 1284 1316 1357 1389 2496
Size: 34 32 41 32 1107
Fragments arranged by size:
1107 442 298 289 93 41 34 32
32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 148 2496
Size: 148 2348
MfeI C'AATT_G
Cuts at: 0 1838 2391 2496
Size: 1838 553 105
Fragments arranged by size:
1838 553 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1439 2496
Size: 388 1051 1057
Fragments arranged by size:
1057 1051 388
MslI CAynn'nnrTG
Cuts at: 0 913 952 1933 2496
Size: 913 39 981 563
Fragments arranged by size:
981 913 563 39
MspA1I CmG'CkG
Cuts at: 0 130 181 258 1575 2496
Size: 130 51 77 1317 921
Fragments arranged by size:
1317 921 130 77 51
NarI GG'CG_CC
Cuts at: 0 149 2496
Size: 149 2347
NcoI C'CATG_G
Cuts at: 0 2300 2496
Size: 2300 196
NdeI CA'TA_TG
Cuts at: 0 1821 2496
Size: 1821 675
PacI TTA_AT'TAA
Cuts at: 0 1646 2496
Size: 1646 850
PfoI T'CCnGG_A
Cuts at: 0 968 2109 2271 2444 2496
Size: 968 1141 162 173 52
Fragments arranged by size:
1141 968 173 162 52
PmlI CAC'GTG
Cuts at: 0 1491 2496
Size: 1491 1005
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1549 1581 2253 2285 2496
Size: 1549 32 672 32 211
Fragments arranged by size:
1549 672 211 32 32
PsiI TTA'TAA
Cuts at: 0 2290 2496
Size: 2290 206
PspOMI G'GGCC_C
Cuts at: 0 61 2496
Size: 61 2435
PstI C_TGCA'G
Cuts at: 0 180 377 1395 2496
Size: 180 197 1018 1101
Fragments arranged by size:
1101 1018 197 180
PvuII CAG'CTG
Cuts at: 0 1575 2496
Size: 1575 921
RsrII CG'GwC_CG
Cuts at: 0 157 2496
Size: 157 2339
SacII CC_GC'GG
Cuts at: 0 259 2496
Size: 259 2237
SapI GCTCTTCn'nnn_
Cuts at: 0 1457 2212 2496
Size: 1457 755 284
Fragments arranged by size:
1457 755 284
SfcI C'TryA_G
Cuts at: 0 176 373 1391 1552 2096 2496
Size: 176 197 1018 161 544 400
Fragments arranged by size:
1018 544 400 197 176 161
SfoI GGC'GCC
Cuts at: 0 150 2496
Size: 150 2346
SmaI CCC'GGG
Cuts at: 0 66 2496
Size: 66 2430
SmlI C'TyrA_G
Cuts at: 0 579 1207 1261 1694 2194 2329 2496
Size: 579 628 54 433 500 135 167
Fragments arranged by size:
628 579 500 433 167 135 54
StyI C'CwwG_G
Cuts at: 0 2300 2496
Size: 2300 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 366 930 2496
Size: 366 564 1566
Fragments arranged by size:
1566 564 366
TatI w'GTAC_w
Cuts at: 0 287 689 2402 2496
Size: 287 402 1713 94
Fragments arranged by size:
1713 402 287 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 596 628 2124 2156 2496
Size: 596 32 1496 32 340
Fragments arranged by size:
1496 596 340 32 32
XmaI C'CCGG_G
Cuts at: 0 64 2496
Size: 64 2432
XmnI GAAnn'nnTTC
Cuts at: 0 1561 2105 2359 2455 2496
Size: 1561 544 254 96 41
Fragments arranged by size:
1561 544 254 96 41
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AleI AloI
AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII
BbeI BbsI BciVI BglI Bme1580I BpmI BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmI
Bsp1286I BspMI BsrBI BsrDI BstAPI BstBI BstXI BstYI
BtgI BtsI DraI DraIII EaeI EagI EarI EciI
Eco57MI EcoNI HaeII Hin4I KasI MfeI MmeI MslI
MspA1I NarI NcoI NdeI PacI PfoI PmlI PpiI
PsiI PspOMI PstI PvuII RsrII SacII SapI SfcI
SfoI SmaI SmlI StyI TaqII TatI TstI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AflII AgeI AhdI AlfI ApaLI AscI
AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaWI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrFI BsrGI BssHII
BssSI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DrdI
EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MluI MscI NaeI NgoMIV
NheI NotI NruI NsiI NspI PasI PciI PflMI
PmeI PpuMI PshAI PspXI PsrI PvuI SacI SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI ZraI