(Circular) (Six-base) MAPSORT of: pFN21AA1887.seq Check: 1967 from: 1 to: 7714 pFN21AA1887 7714 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2372 2665 2901 2372 Size: 293 236 7185 Fragments arranged by size: 7185 293 236 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5524 278 Size: 53 83 186 936 3988 2468 Fragments arranged by size: 3988 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4441 5057 1937 Size: 2504 616 4594 Fragments arranged by size: 4594 2504 616 Acc65I G'GTAC_C Cuts at: 5035 5035 Size: 7714 AclI AA'CG_TT Cuts at: 1563 5841 6214 1563 Size: 4278 373 3063 Fragments arranged by size: 4278 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2754 2862 3801 3831 4785 5777 7161 7429 Size: 1122 108 939 30 954 992 1384 268 Cuts at: 7429 1632 Size: 1917 Fragments arranged by size: 1917 1384 1122 992 954 939 268 108 30 AfeI AGC'GCT Cuts at: 2084 2084 Size: 7714 AflII C'TTAA_G Cuts at: 820 1017 4820 820 Size: 197 3803 3714 Fragments arranged by size: 3803 3714 197 AflIII A'CryG_T Cuts at: 1203 4688 6613 7590 1203 Size: 3485 1925 977 1327 Fragments arranged by size: 3485 1925 1327 977 AgeI A'CCGG_T Cuts at: 6545 6545 Size: 7714 AhdI GACnn_n'nnGTC Cuts at: 6443 6443 Size: 7714 AleI CACnn'nnGTG Cuts at: 1166 2793 1166 Size: 1627 6087 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7714 AlwNI CAG_nnn'CTG Cuts at: 2546 3583 4481 4541 5457 7029 2546 Size: 1037 898 60 916 1572 3231 Fragments arranged by size: 3231 1572 1037 916 898 60 ApaI G_GGCC'C Cuts at: 2051 2430 2913 2926 3178 3902 4806 4969 2051 Size: 379 483 13 252 724 904 163 4796 Fragments arranged by size: 4796 904 724 483 379 252 163 13 ApaLI G'TGCA_C Cuts at: 5771 6927 5771 Size: 1156 6558 ApoI r'AATT_y Cuts at: 1493 4033 5023 5249 5401 7510 1493 Size: 2540 990 226 152 2109 1697 Fragments arranged by size: 2540 2109 1697 990 226 152 AseI AT'TA_AT Cuts at: 160 1032 4608 6268 160 Size: 872 3576 1660 1606 Fragments arranged by size: 3576 1660 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7714 AvaI C'yCGr_G Cuts at: 1870 1943 2254 2824 3171 4069 4153 4448 4629 Size: 73 311 570 347 898 84 295 181 Cuts at: 4629 4799 4831 4938 4962 5039 1870 Size: 170 32 107 24 77 4545 Fragments arranged by size: 4545 898 570 347 311 295 181 170 107 84 77 73 32 24 BamHI G'GATC_C Cuts at: 4060 4786 5044 5411 4060 Size: 726 258 367 6363 Fragments arranged by size: 6363 726 367 258 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2716 2947 3052 3366 3425 Size: 325 217 627 929 231 105 314 59 Cuts at: 3425 4226 4825 5035 6490 618 Size: 801 599 210 1455 1842 Fragments arranged by size: 1842 1455 929 801 627 599 325 314 231 217 210 105 59 BanII G_rGCy'C Cuts at: 1391 2051 2399 2430 2735 2913 2926 3178 3353 Size: 660 348 31 305 178 13 252 175 Cuts at: 3353 3729 3902 4276 4354 4457 4782 4806 4969 Size: 376 173 374 78 103 325 24 163 Cuts at: 4969 5033 1391 Size: 64 4072 Fragments arranged by size: 4072 660 376 374 348 325 305 252 178 175 173 163 103 78 64 31 24 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 2868 4242 928 Size: 971 231 738 1374 4400 Fragments arranged by size: 4400 1374 971 738 231 BbvCI CC'TCA_GC Cuts at: 2168 3058 3410 4462 2168 Size: 890 352 1052 5420 Fragments arranged by size: 5420 1052 890 352 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4439 4473 5905 5939 1903 Size: 34 2502 34 1432 34 3678 Fragments arranged by size: 3678 2502 1432 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5606 6816 5606 Size: 1210 6504 BclI T'GATC_A Cuts at: 1548 1548 Size: 7714 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3784 4486 6325 Size: 107 122 71 631 605 2112 702 1839 Cuts at: 6325 136 Size: 1525 Fragments arranged by size: 2112 1839 1525 702 631 605 122 107 71 BglII A'GATC_T Cuts at: 7709 7709 Size: 7714 BlpI GC'TnA_GC Cuts at: 4776 5131 4776 Size: 355 7359 Bme1580I G_kGCm'C Cuts at: 1790 2051 2430 2721 2913 2926 2950 3049 3178 Size: 261 379 291 192 13 24 99 129 Cuts at: 3178 3430 3902 4105 4806 4830 4969 5775 6931 Size: 252 472 203 701 24 139 806 1156 Cuts at: 6931 1790 Size: 2573 Fragments arranged by size: 2573 1156 806 701 472 379 291 261 252 203 192 139 129 99 24 24 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7714 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2573 2698 3452 Size: 416 53 83 399 24 1156 125 754 Cuts at: 3452 3509 3708 4529 4563 5006 5445 6403 442 Size: 57 199 821 34 443 439 958 1753 Fragments arranged by size: 1753 1156 958 821 754 443 439 416 399 199 125 83 57 53 34 24 BmtI G_CTAG'C Cuts at: 1056 2967 1056 Size: 1911 5803 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2717 3162 3186 2717 Size: 445 24 7245 Fragments arranged by size: 7245 445 24 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2403 2622 4111 4290 4391 4401 6374 Size: 977 187 219 1489 179 101 10 1973 Cuts at: 6374 1239 Size: 2579 Fragments arranged by size: 2579 1973 1489 977 219 187 179 101 10 Bpu10I CC'TnA_GC Cuts at: 2168 3058 3410 3573 3723 4211 4462 4504 4542 Size: 890 352 163 150 488 251 42 38 Cuts at: 4542 2168 Size: 5340 Fragments arranged by size: 5340 890 488 352 251 163 150 42 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5839 6704 7002 5839 Size: 865 298 6551 Fragments arranged by size: 6551 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2351 3969 4189 6377 882 Size: 617 852 1618 220 2188 2219 Fragments arranged by size: 2219 2188 1618 852 617 220 BsaAI yAC'GTr Cuts at: 493 1204 4691 5505 493 Size: 711 3487 814 2702 Fragments arranged by size: 3487 2702 814 711 BsaBI GATnn'nnATC Cuts at: 7708 7708 Size: 7714 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5521 5903 6538 275 Size: 53 83 186 936 3988 382 635 1451 Fragments arranged by size: 3988 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6147 6545 6819 6966 7377 6147 Size: 398 274 147 411 6484 Fragments arranged by size: 6484 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2797 2827 3213 3243 4389 Size: 30 165 30 1394 30 386 30 1146 Cuts at: 4389 4419 1178 Size: 30 4473 Fragments arranged by size: 4473 1394 1146 386 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2300 2640 2727 2746 2749 2769 3061 Size: 970 144 340 87 19 3 20 292 Cuts at: 3061 3315 3331 3720 4153 4325 4344 4602 5011 Size: 254 16 389 433 172 19 258 409 Cuts at: 5011 1186 Size: 3889 Fragments arranged by size: 3889 970 433 409 389 340 292 258 254 172 144 87 20 19 19 16 3 BseYI C'CCAG_C Cuts at: 2042 2264 3017 3250 3357 3467 3566 3857 3908 Size: 222 753 233 107 110 99 291 51 Cuts at: 3908 4083 4222 4237 4280 4925 6917 7413 2042 Size: 175 139 15 43 645 1992 496 2343 Fragments arranged by size: 2343 1992 753 645 496 291 233 222 175 139 110 107 99 51 43 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5164 5925 6953 1540 Size: 275 186 3163 761 1028 2301 Fragments arranged by size: 3163 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2109 4354 5775 5860 6931 2109 Size: 2245 1421 85 1071 2892 Fragments arranged by size: 2892 2245 1421 1071 85 BsmI GAATG_Cn' Cuts at: 5225 5318 5225 Size: 93 7621 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2109 2399 2430 2721 2735 2913 Size: 399 261 58 290 31 291 14 178 Cuts at: 2913 2926 2950 3049 3178 3353 3430 3729 3902 Size: 13 24 99 129 175 77 299 173 Cuts at: 3902 4105 4276 4354 4457 4782 4806 4830 4969 Size: 203 171 78 103 325 24 24 139 Cuts at: 4969 5033 5775 5860 6931 1391 Size: 64 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 325 299 291 290 261 203 178 175 173 171 139 129 103 99 85 78 77 64 58 31 24 24 24 14 13 BspEI T'CCGG_A Cuts at: 7377 7377 Size: 7714 BspHI T'CATG_A Cuts at: 5603 7333 5603 Size: 1730 5984 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2189 2372 2665 2901 5069 844 Size: 1345 183 293 236 2168 3489 Fragments arranged by size: 3489 2168 1345 293 236 183 BsrBI CCG'CTC Cuts at: 1394 1823 5601 1394 Size: 429 3778 3507 Fragments arranged by size: 3778 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2023 2632 3665 6209 6383 65 Size: 1183 775 609 1033 2544 174 1396 Fragments arranged by size: 2544 1396 1183 1033 775 609 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2313 4650 6358 6545 1700 Size: 110 503 2337 1708 187 2869 Fragments arranged by size: 2869 2337 1708 503 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7714 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7714 BssSI C'ACGA_G Cuts at: 5774 6786 5774 Size: 1012 6702 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7714 BstBI TT'CG_AA Cuts at: 6518 6518 Size: 7714 BstEII G'GTnAC_C Cuts at: 1184 2248 4241 1184 Size: 1064 1993 4657 Fragments arranged by size: 4657 1993 1064 BstXI CCAn_nnnn'nTGG Cuts at: 3284 4237 3284 Size: 953 6761 BstYI r'GATC_y Cuts at: 1972 2705 3923 4060 4410 4786 5044 5411 5796 Size: 733 1218 137 350 376 258 367 385 Cuts at: 5796 5813 7265 7351 7363 7709 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1218 733 385 376 367 350 346 258 137 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3343 3343 Size: 7714 BtgI C'CryG_G Cuts at: 513 1065 2928 4137 4516 513 Size: 552 1863 1209 379 3711 Fragments arranged by size: 3711 1863 1209 552 379 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2187 487 Size: 683 56 961 6014 Fragments arranged by size: 6014 961 683 56 BtsI GCAGTG_nn' Cuts at: 2032 3421 5230 6024 6044 2032 Size: 1389 1809 794 20 3702 Fragments arranged by size: 3702 1809 1389 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7714 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7679 DraI TTT'AAA Cuts at: 4032 5018 4032 Size: 986 6728 DraIII CAC_nnn'GTG Cuts at: 2416 3593 4694 2416 Size: 1177 1101 5436 Fragments arranged by size: 5436 1177 1101 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3165 4535 6721 809 Size: 507 1849 1370 2186 1802 Fragments arranged by size: 2186 1849 1802 1370 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2985 4315 Size: 54 1425 50 275 18 225 930 1330 Cuts at: 4315 4663 4858 4975 5161 6050 7679 8 Size: 348 195 117 186 889 1629 43 Fragments arranged by size: 1629 1425 1330 930 889 348 275 225 195 186 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5161 1537 Size: 275 3349 4090 Fragments arranged by size: 4090 3349 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2380 2789 4311 5644 1353 Size: 1027 409 1522 1333 3423 Fragments arranged by size: 3423 1522 1333 1027 409 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2332 6301 6675 6821 387 Size: 1064 138 743 3969 374 146 1280 Fragments arranged by size: 3969 1280 1064 743 374 146 138 EcoICRI GAG'CTC Cuts at: 4352 4352 Size: 7714 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2403 2622 2754 2862 3801 3831 Size: 393 584 187 219 132 108 939 30 Cuts at: 3831 4111 4290 4391 4401 4785 5777 6374 7161 Size: 280 179 101 10 384 992 597 787 Cuts at: 7161 7429 1239 Size: 268 1524 Fragments arranged by size: 1524 992 939 787 597 584 393 384 280 268 219 187 179 132 108 101 30 10 EcoNI CCTnn'n_nnAGG Cuts at: 4367 4367 Size: 7714 EcoO109I rG'GnC_Cy Cuts at: 2047 2048 2178 2426 2427 2483 2543 2909 2910 Size: 1 130 248 1 56 60 366 1 Cuts at: 2910 2922 2923 2939 3039 3143 3175 3580 3621 Size: 12 1 16 100 104 32 405 41 Cuts at: 3621 3749 3785 3899 4158 4177 4719 4803 4816 Size: 128 36 114 259 19 542 84 13 Cuts at: 4816 2047 Size: 4945 Fragments arranged by size: 4945 542 405 366 259 248 130 128 114 104 100 84 60 56 41 36 32 19 16 13 12 1 1 1 1 EcoRI G'AATT_C Cuts at: 5023 5401 5023 Size: 378 7336 FspI TGC'GCA Cuts at: 6220 6220 Size: 7714 HaeII r_GCGC'y Cuts at: 1678 2086 6861 1678 Size: 408 4775 2531 Fragments arranged by size: 4775 2531 408 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2717 2749 2818 2850 4052 Size: 32 135 32 1145 32 69 32 1202 Cuts at: 4052 4084 4659 4691 4932 4964 6355 6387 6429 Size: 32 575 32 241 32 1391 32 42 Cuts at: 6429 6461 1373 Size: 32 2626 Fragments arranged by size: 2626 1391 1202 1145 575 241 135 69 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5058 5304 669 Size: 988 281 3120 246 3079 Fragments arranged by size: 3120 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3289 748 Size: 2541 5173 HpaI GTT'AAC Cuts at: 5304 5304 Size: 7714 KpnI G_GTAC'C Cuts at: 5039 5039 Size: 7714 MfeI C'AATT_G Cuts at: 5313 5313 Size: 7714 MluI A'CGCG_T Cuts at: 7590 7590 Size: 7714 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4209 6828 7012 1305 Size: 2904 2619 184 2007 Fragments arranged by size: 2904 2619 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 4317 10 Size: 54 1425 343 225 2260 3407 Fragments arranged by size: 3407 2260 1425 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2793 3282 5672 6031 6190 518 Size: 648 1627 489 2390 359 159 2042 Fragments arranged by size: 2390 2042 1627 648 489 359 159 MspA1I CmG'CkG Cuts at: 1606 2984 3443 3908 4946 5130 5807 6955 7200 Size: 1378 459 465 1038 184 677 1148 245 Cuts at: 7200 1606 Size: 2120 Fragments arranged by size: 2120 1378 1148 1038 677 465 459 245 184 NaeI GCC'GGC Cuts at: 4652 4652 Size: 7714 NcoI C'CATG_G Cuts at: 513 1065 4516 513 Size: 552 3451 3711 Fragments arranged by size: 3711 3451 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7714 NgoMIV G'CCGG_C Cuts at: 4650 4650 Size: 7714 NheI G'CTAG_C Cuts at: 1052 2963 1052 Size: 1911 5803 NotI GC'GGCC_GC Cuts at: 5161 5161 Size: 7714 NspI r_CATG'y Cuts at: 1132 1229 5072 6617 1132 Size: 97 3843 1545 2229 Fragments arranged by size: 3843 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2423 2546 2914 3608 4340 1348 Size: 1075 123 368 694 732 4722 Fragments arranged by size: 4722 1075 732 694 368 123 PciI A'CATG_T Cuts at: 6613 6613 Size: 7714 PflMI CCAn_nnn'nTGG Cuts at: 1108 2076 2477 4517 1108 Size: 968 401 2040 4305 Fragments arranged by size: 4305 2040 968 401 PfoI T'CCnGG_A Cuts at: 2380 3207 2380 Size: 827 6887 PmeI GTTT'AAAC Cuts at: 5018 5018 Size: 7714 PmlI CAC'GTG Cuts at: 4691 4691 Size: 7714 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5783 5815 7296 7328 5783 Size: 32 1481 32 6169 Fragments arranged by size: 6169 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2178 2483 3749 4719 4816 2178 Size: 305 1266 970 97 5076 Fragments arranged by size: 5076 1266 970 305 97 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7714 PsiI TTA'TAA Cuts at: 5284 5284 Size: 7714 PspOMI G'GGCC_C Cuts at: 2047 2426 2909 2922 3174 3898 4802 4965 2047 Size: 379 483 13 252 724 904 163 4796 Fragments arranged by size: 4796 904 724 483 379 252 163 13 PstI C_TGCA'G Cuts at: 830 2983 3931 4332 5066 830 Size: 2153 948 401 734 3478 Fragments arranged by size: 3478 2153 948 734 401 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7714 PvuII CAG'CTG Cuts at: 3443 3908 4946 3443 Size: 465 1038 6211 Fragments arranged by size: 6211 1038 465 SacI G_AGCT'C Cuts at: 4354 4354 Size: 7714 SalI G'TCGA_C Cuts at: 1936 5056 1936 Size: 3120 4594 SbfI CC_TGCA'GG Cuts at: 5066 5066 Size: 7714 ScaI AGT'ACT Cuts at: 5962 7674 5962 Size: 1712 6002 SexAI A'CCwGG_T Cuts at: 3751 3751 Size: 7714 SfcI C'TryA_G Cuts at: 826 1045 2791 2979 3927 4328 4397 4932 5062 Size: 219 1746 188 948 401 69 535 130 Cuts at: 5062 6197 6878 7069 826 Size: 1135 681 191 1471 Fragments arranged by size: 1746 1471 1135 948 681 535 401 219 191 188 130 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7714 SgrAI Cr'CCGG_yG Cuts at: 1700 2313 1700 Size: 613 7101 SmaI CCC'GGG Cuts at: 1872 2256 4155 4631 4801 4833 4964 5041 1872 Size: 384 1899 476 170 32 131 77 4545 Fragments arranged by size: 4545 1899 476 384 170 131 77 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 4820 5818 6719 6981 820 Size: 197 926 2877 998 901 262 1553 Fragments arranged by size: 2877 1553 998 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5505 493 Size: 5012 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7714 SphI G_CATG'C Cuts at: 1132 5072 1132 Size: 3940 3774 SspI AAT'ATT Cuts at: 5 52 5638 5 Size: 47 5586 2081 Fragments arranged by size: 5586 2081 47 StuI AGG'CCT Cuts at: 1517 2525 4489 1517 Size: 1008 1964 4742 Fragments arranged by size: 4742 1964 1008 StyI C'CwwG_G Cuts at: 513 1065 2142 2217 2564 2618 2988 4516 4782 Size: 552 1077 75 347 54 370 1528 266 Cuts at: 4782 4917 5153 513 Size: 135 236 3074 Fragments arranged by size: 3074 1528 1077 552 370 347 266 236 135 75 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4214 4229 5741 5758 5911 6096 6541 Size: 1255 2634 15 1512 17 153 185 445 Cuts at: 6541 325 Size: 1498 Fragments arranged by size: 2634 1512 1498 1255 445 185 153 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5960 7672 96 Size: 274 80 33 51 1443 3983 1712 138 Fragments arranged by size: 3983 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2196 2228 2402 2434 2940 2972 3385 3417 4045 Size: 32 174 32 506 32 413 32 628 Cuts at: 4045 4077 4678 4710 7674 7706 2196 Size: 32 601 32 2964 32 2204 Fragments arranged by size: 2964 2204 628 601 506 413 174 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2579 3753 1316 Size: 1263 1174 5277 Fragments arranged by size: 5277 1263 1174 XbaI T'CTAG_A Cuts at: 5050 5050 Size: 7714 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3683 3683 Size: 7714 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7714 XmaI C'CCGG_G Cuts at: 1870 2254 4153 4629 4799 4831 4962 5039 1870 Size: 384 1899 476 170 32 131 77 4545 Fragments arranged by size: 4545 1899 476 384 170 131 77 32 XmnI GAAnn'nnTTC Cuts at: 5843 5843 Size: 7714 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5522 276 Size: 53 83 186 936 3988 2468 Fragments arranged by size: 3988 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI