Restriction map for
pFN21AA1849
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI AocI ApoI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BclI BfrI
BglII BlnI BsaBI BsiWI BsmBI
BsmI BspDI BspLU11I BsrDI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HindIII HpaI
KpnI MamI MfeI MluI MseI
MunI NdeI NheI NotI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1705 GTCTAC GT^[AC][GT]AC
AccIII 2829 TCCGGA T^CCGGA
Alw44I 1876 GTGCAC G^TGCAC
Aor51HI 2778 AGCGCT AGC^GCT
ApaLI 1876 GTGCAC G^TGCAC
AscI 1101 GGCGCGCC GG^CGCGCC
AspI 208 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2503 CACGTG CAC^GTG
BbsI 2914 GTCTTC GAAGAC(2/6)
BbvCI 474 GCTGAGG CCTCAGC(-5/-2)
BcgI 561 GCAGCGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3068 GTATCC GTATCC(6/5)
BfaI 1426 CTAG C^TAG
BlpI 93 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 93 GCTGAGC GC^T[ACGT]AGC
BpuAI 2914 GTCTTC GAAGAC(2/6)
BsaI 649 GAGACC GGTCTC(1/5)
BsaWI 2829 TCCGGA [AT]^CCGG[AT]
BseAI 2829 TCCGGA T^CCGGA
Bsp1407I 1727 TGTACA T^GTACA
BspEI 2829 TCCGGA T^CCGGA
BspHI 1256 TCATGA T^CATGA
BsrGI 1727 TGTACA T^GTACA
BssHII 1102 GCGCGC G^CGCGC
BstYI 3194 AGATCC [GA]^GATC[CT]
CelII 93 GCTGAGC GC^T[ACGT]AGC
Eco47III 2778 AGCGCT AGC^GCT
FseI 318 GGCCGGCC GGCCGG^CC
HincII 973 GTCAAC GT[CT]^[GA]AC
HindII 973 GTCAAC GT[CT]^[GA]AC
KspI 926 CCGCGG CCGC^GG
MaeI 1426 CTAG C^TAG
MaeIII 338 GTGAC ^GT[ACGT]AC
MflI 3194 AGATCC [GA]^GATC[CT]
MroI 2829 TCCGGA T^CCGGA
NruI 3164 TCGCGA TCG^CGA
PflFI 208 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2503 CACGTG CAC^GTG
PmlI 2503 CACGTG CAC^GTG
RcaI 1256 TCATGA T^CATGA
SacII 926 CCGCGG CCGC^GG
SphI 77 GCATGC GCATG^C
SspBI 1727 TGTACA T^GTACA
TfiI 1618 GAATC G^A[AT]TC
Tsp45I 338 GTGAC ^GT[GC]AC
Tsp509I 434 AATT ^AATT
Tth111I 208 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 3194 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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BsaAI 328 TACGTG [CT]AC^GT[GA]
2503 CACGTG
BsiEI 2649 CGGCCG CG[GA][CT]^CG
3038 CGGCCG
BsrBI 904 CCGCTC CCGCTC(-3/-3)
292 GAGCGG
DraIII 812 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
2500 CACCACGTG
EagI 2649 CGGCCG C^GGCCG
3038 CGGCCG
EclXI 2649 CGGCCG C^GGCCG
3038 CGGCCG
Eco52I 2649 CGGCCG C^GGCCG
3038 CGGCCG
EcoNI 756 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2850 CCTTGCGGAGG
HphI 276 GGTGA GGTGA(8/7)
3078 GGTGA
NspI 77 GCATGC [GA]CATG^[CT]
1574 ACATGC
SmlI 1375 CTCAAG C^T[CT][GA]AG
2604 CTTGAG
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI KasI Ksp632I MaeII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StuI StyI TaqI TseI
TspRI TthHB8I XcmI XmaCI XmaI