(Circular) (Six-base) MAPSORT of: pFN21AA1643.seq Check: 1207 from: 1 to: 7363 pFN21AA1643 7363 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4576 4576 Size: 7363 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2499 5173 278 Size: 53 83 186 936 963 2674 2468 Fragments arranged by size: 2674 2468 963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3160 4706 1937 Size: 1223 1546 4594 Fragments arranged by size: 4594 1546 1223 Acc65I G'GTAC_C Cuts at: 2628 4684 2628 Size: 2056 5307 AclI AA'CG_TT Cuts at: 1563 5490 5863 1563 Size: 3927 373 3063 Fragments arranged by size: 3927 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5426 6810 7078 1632 Size: 3794 1384 268 1917 Fragments arranged by size: 3794 1917 1384 268 AfeI AGC'GCT Cuts at: 2481 2481 Size: 7363 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7166 AflIII A'CryG_T Cuts at: 1203 2757 2784 4620 6262 7239 1203 Size: 1554 27 1836 1642 977 1327 Fragments arranged by size: 1836 1642 1554 1327 977 27 AgeI A'CCGG_T Cuts at: 6194 6194 Size: 7363 AhdI GACnn_n'nnGTC Cuts at: 3067 6092 3067 Size: 3025 4338 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7363 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2076 2387 1184 Size: 892 311 6160 Fragments arranged by size: 6160 892 311 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3185 3217 3185 Size: 32 7331 AlwNI CAG_nnn'CTG Cuts at: 2440 2821 3025 3288 3435 4039 4336 5106 6678 Size: 381 204 263 147 604 297 770 1572 Cuts at: 6678 2440 Size: 3125 Fragments arranged by size: 3125 1572 770 604 381 297 263 204 147 ApaI G_GGCC'C Cuts at: 2617 3820 2617 Size: 1203 6160 ApaLI G'TGCA_C Cuts at: 2146 4362 4606 5420 6576 2146 Size: 2216 244 814 1156 2933 Fragments arranged by size: 2933 2216 1156 814 244 ApoI r'AATT_y Cuts at: 1493 4672 4898 5050 7159 1493 Size: 3179 226 152 2109 1697 Fragments arranged by size: 3179 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5917 160 Size: 872 4885 1606 Fragments arranged by size: 4885 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7363 AvaI C'yCGr_G Cuts at: 1870 1943 3248 3389 3760 4688 1870 Size: 73 1305 141 371 928 4545 Fragments arranged by size: 4545 1305 928 371 141 73 BamHI G'GATC_C Cuts at: 2047 4693 5060 2047 Size: 2646 367 4350 Fragments arranged by size: 4350 2646 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2312 2420 2539 2628 2920 Size: 325 217 627 525 108 119 89 292 Cuts at: 2920 3425 3776 3838 4027 4444 4684 6139 618 Size: 505 351 62 189 417 240 1455 1842 Fragments arranged by size: 1842 1455 627 525 505 417 351 325 292 240 217 189 119 108 89 62 BanII G_rGCy'C Cuts at: 1391 2520 2617 3621 3820 3879 4013 4049 4682 Size: 1129 97 1004 199 59 134 36 633 Cuts at: 4682 1391 Size: 4072 Fragments arranged by size: 4072 1129 1004 633 199 134 97 59 36 BbeI G_GCGC'C Cuts at: 2424 2924 3842 4031 2424 Size: 500 918 189 5756 Fragments arranged by size: 5756 918 500 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2760 4434 928 Size: 971 861 1674 3857 Fragments arranged by size: 3857 1674 971 861 BbvCI CC'TCA_GC Cuts at: 2483 2483 Size: 7363 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3928 3962 5554 5588 1903 Size: 34 1991 34 1592 34 3678 Fragments arranged by size: 3678 1991 1592 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5255 6465 5255 Size: 1210 6153 BclI T'GATC_A Cuts at: 1548 1548 Size: 7363 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3423 3765 4130 Size: 107 122 71 631 605 1751 342 365 Cuts at: 4130 5974 136 Size: 1844 1525 Fragments arranged by size: 1844 1751 1525 631 605 365 342 122 107 71 BglII A'GATC_T Cuts at: 4533 7358 4533 Size: 2825 4538 BlpI GC'TnA_GC Cuts at: 2519 3035 3656 3796 4780 2519 Size: 516 621 140 984 5102 Fragments arranged by size: 5102 984 621 516 140 Bme1580I G_kGCm'C Cuts at: 1790 2150 2617 3404 3428 3820 4366 4610 5424 Size: 360 467 787 24 392 546 244 814 Cuts at: 5424 6580 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 814 787 546 467 392 360 244 24 BmgBI CAC'GTC Cuts at: 1318 2413 2875 2899 3217 3730 1318 Size: 1095 462 24 318 513 4951 Fragments arranged by size: 4951 1095 513 462 318 24 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 5094 6052 Size: 416 53 83 399 24 2255 1422 958 Cuts at: 6052 442 Size: 1753 Fragments arranged by size: 2255 1753 1422 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7363 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3088 3628 3088 Size: 540 6823 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 2426 3047 3138 4033 6023 1239 Size: 1139 48 621 91 895 1990 2579 Fragments arranged by size: 2579 1990 1139 895 621 91 48 Bpu10I CC'TnA_GC Cuts at: 2483 2967 2483 Size: 484 6879 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3394 5488 6353 6651 3394 Size: 2094 865 298 4106 Fragments arranged by size: 4106 2094 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4370 6026 882 Size: 617 2871 1656 2219 Fragments arranged by size: 2871 2219 1656 617 BsaAI yAC'GTr Cuts at: 493 1204 2569 5154 493 Size: 711 1365 2585 2702 Fragments arranged by size: 2702 2585 1365 711 BsaBI GATnn'nnATC Cuts at: 7357 7357 Size: 7363 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2421 2496 2921 3768 Size: 53 83 186 936 888 75 425 847 Cuts at: 3768 3839 4028 5170 5552 6187 275 Size: 71 189 1142 382 635 1451 Fragments arranged by size: 1451 1142 936 888 847 635 425 382 189 186 83 75 71 53 BsaWI w'CCGG_w Cuts at: 3519 4149 5796 6194 6468 6615 7026 3519 Size: 630 1647 398 274 147 411 3856 Fragments arranged by size: 3856 1647 630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3185 3215 4367 4397 1178 Size: 30 165 30 1782 30 1152 30 4144 Fragments arranged by size: 4144 1782 1152 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2285 2843 2846 3203 3206 3380 3848 Size: 913 186 558 3 357 3 174 468 Cuts at: 3848 4066 4543 1186 Size: 218 477 4006 Fragments arranged by size: 4006 913 558 477 468 357 218 186 174 3 3 BseYI C'CCAG_C Cuts at: 2139 3106 3256 3471 3959 4004 4064 4105 6566 Size: 967 150 215 488 45 60 41 2461 Cuts at: 6566 7062 2139 Size: 496 2440 Fragments arranged by size: 2461 2440 967 496 488 215 150 60 45 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2591 2822 2921 3443 3775 4453 1163 Size: 573 855 231 99 522 332 678 4073 Fragments arranged by size: 4073 855 678 573 522 332 231 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3594 4813 5574 6602 1540 Size: 275 186 1593 1219 761 1028 2301 Fragments arranged by size: 2301 1593 1219 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2150 2520 2592 4366 4610 5424 5509 6580 2150 Size: 370 72 1774 244 814 85 1071 2933 Fragments arranged by size: 2933 1774 1071 814 370 244 85 72 BsiWI C'GTAC_G Cuts at: 3783 3783 Size: 7363 BsmI GAATG_Cn' Cuts at: 3685 4874 4967 3685 Size: 1189 93 6081 Fragments arranged by size: 6081 1189 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2533 2594 3491 2533 Size: 61 897 6405 Fragments arranged by size: 6405 897 61 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2150 2520 2592 2617 3404 3428 3621 Size: 399 360 370 72 25 787 24 193 Cuts at: 3621 3820 3879 4013 4049 4366 4610 4682 5424 Size: 199 59 134 36 317 244 72 742 Cuts at: 5424 5509 6580 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 787 742 399 370 360 317 244 199 193 134 85 72 72 59 36 25 24 BspEI T'CCGG_A Cuts at: 7026 7026 Size: 7363 BspHI T'CATG_A Cuts at: 2343 5252 6982 2343 Size: 2909 1730 2724 Fragments arranged by size: 2909 2724 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3959 4576 4718 844 Size: 3115 617 142 3489 Fragments arranged by size: 3489 3115 617 142 BsrBI CCG'CTC Cuts at: 1394 1823 2172 3125 3332 5250 1394 Size: 429 349 953 207 1918 3507 Fragments arranged by size: 3507 1918 953 429 349 207 BsrDI GCAATG_nn' Cuts at: 65 1248 3090 5858 6032 65 Size: 1183 1842 2768 174 1396 Fragments arranged by size: 2768 1842 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2294 2330 2662 3791 4594 4655 6007 Size: 110 484 36 332 1129 803 61 1352 Cuts at: 6007 6194 1700 Size: 187 2869 Fragments arranged by size: 2869 1352 1129 803 484 332 187 110 61 36 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7363 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7363 BssSI C'ACGA_G Cuts at: 2708 4022 5423 6435 2708 Size: 1314 1401 1012 3636 Fragments arranged by size: 3636 1401 1314 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4476 4614 794 Size: 3682 138 3543 Fragments arranged by size: 3682 3543 138 BstBI TT'CG_AA Cuts at: 6167 6167 Size: 7363 BstEII G'GTnAC_C Cuts at: 1184 4324 1184 Size: 3140 4223 BstXI CCAn_nnnn'nTGG Cuts at: 3273 3580 3273 Size: 307 7056 BstYI r'GATC_y Cuts at: 1972 2047 2274 4146 4533 4693 5060 5445 5462 Size: 75 227 1872 387 160 367 385 17 Cuts at: 5462 6914 7000 7012 7358 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1872 1452 387 385 367 346 227 160 86 75 17 12 Bsu36I CC'TnA_GG Cuts at: 2268 2949 3916 4032 2268 Size: 681 967 116 5599 Fragments arranged by size: 5599 967 681 116 BtgI C'CryG_G Cuts at: 513 1065 2103 2535 3546 3621 4162 513 Size: 552 1038 432 1011 75 541 3714 Fragments arranged by size: 3714 1038 1011 552 541 432 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4428 487 Size: 683 56 3202 3422 Fragments arranged by size: 3422 3202 683 56 BtsI GCAGTG_nn' Cuts at: 3187 4513 4612 4879 5673 5693 3187 Size: 1326 99 267 794 20 4857 Fragments arranged by size: 4857 1326 794 267 99 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7363 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7328 DraI TTT'AAA Cuts at: 4667 4667 Size: 7363 DraIII CAC_nnn'GTG Cuts at: 4476 4476 Size: 7363 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3590 6370 809 Size: 507 2274 2780 1802 Fragments arranged by size: 2780 2274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2292 2296 Size: 54 1425 50 275 18 270 192 4 Cuts at: 2296 2325 2367 2382 2532 2691 3240 3624 4281 Size: 29 42 15 150 159 549 384 657 Cuts at: 4281 4810 5699 7328 8 Size: 529 889 1629 43 Fragments arranged by size: 1629 1425 889 657 549 529 384 275 270 192 159 150 54 50 43 42 29 18 15 4 EagI C'GGCC_G Cuts at: 1537 1812 4810 1537 Size: 275 2998 4090 Fragments arranged by size: 4090 2998 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2597 5293 1353 Size: 1244 2696 3423 Fragments arranged by size: 3423 2696 1244 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2143 3342 4490 5950 6324 6470 Size: 1064 138 554 1199 1148 1460 374 146 Cuts at: 6470 387 Size: 1280 Fragments arranged by size: 1460 1280 1199 1148 1064 554 374 146 138 EcoICRI GAG'CTC Cuts at: 2518 2518 Size: 7363 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 2426 3047 3138 4033 5426 6023 Size: 393 746 48 621 91 895 1393 597 Cuts at: 6023 6810 7078 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1393 895 787 746 621 597 393 268 91 48 EcoNI CCTnn'n_nnAGG Cuts at: 2636 2636 Size: 7363 EcoO109I rG'GnC_Cy Cuts at: 2134 2446 2613 2614 2906 3816 3817 4036 4068 Size: 312 167 1 292 910 1 219 32 Cuts at: 4068 2134 Size: 5429 Fragments arranged by size: 5429 910 312 292 219 167 32 1 1 EcoRI G'AATT_C Cuts at: 4672 5050 4672 Size: 378 6985 FseI GG_CCGG'CC Cuts at: 2298 4659 2298 Size: 2361 5002 FspI TGC'GCA Cuts at: 5869 5869 Size: 7363 HaeII r_GCGC'y Cuts at: 1678 2424 2441 2483 2924 3842 4031 6510 1678 Size: 746 17 42 441 918 189 2479 2531 Fragments arranged by size: 2531 2479 918 746 441 189 42 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2032 2064 3053 3085 4131 Size: 32 135 32 460 32 989 32 1046 Cuts at: 4131 4163 4367 4399 6004 6036 6078 6110 1373 Size: 32 204 32 1605 32 42 32 2626 Fragments arranged by size: 2626 1605 1046 989 460 204 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4707 4953 669 Size: 988 281 2769 246 3079 Fragments arranged by size: 3079 2769 988 281 246 HindIII A'AGCT_T Cuts at: 748 3653 748 Size: 2905 4458 HpaI GTT'AAC Cuts at: 4953 4953 Size: 7363 KasI G'GCGC_C Cuts at: 2420 2920 3838 4027 2420 Size: 500 918 189 5756 Fragments arranged by size: 5756 918 500 189 KpnI G_GTAC'C Cuts at: 2632 4688 2632 Size: 2056 5307 MfeI C'AATT_G Cuts at: 4962 4962 Size: 7363 MluI A'CGCG_T Cuts at: 7239 7239 Size: 7363 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2025 3888 3918 6477 6661 1305 Size: 720 1863 30 2559 184 2007 Fragments arranged by size: 2559 2007 1863 720 184 30 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 2384 2693 3242 4283 Size: 54 1425 343 537 15 309 549 1041 Cuts at: 4283 10 Size: 3090 Fragments arranged by size: 3090 1425 1041 549 537 343 309 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2342 2369 3271 5321 5680 5839 518 Size: 648 1176 27 902 2050 359 159 2042 Fragments arranged by size: 2050 2042 1176 902 648 359 159 27 MspA1I CmG'CkG Cuts at: 1606 2056 2105 2143 2302 2380 2912 3260 3441 Size: 450 49 38 159 78 532 348 181 Cuts at: 3441 4064 4222 4524 4570 4779 5456 6604 6849 Size: 623 158 302 46 209 677 1148 245 Cuts at: 6849 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 623 532 450 348 302 245 209 181 159 158 78 49 46 38 NaeI GCC'GGC Cuts at: 2296 2332 2664 3793 4657 2296 Size: 36 332 1129 864 5002 Fragments arranged by size: 5002 1129 864 332 36 NarI GG'CG_CC Cuts at: 2421 2921 3839 4028 2421 Size: 500 918 189 5756 Fragments arranged by size: 5756 918 500 189 NcoI C'CATG_G Cuts at: 513 1065 2535 513 Size: 552 1470 5341 Fragments arranged by size: 5341 1470 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7363 NgoMIV G'CCGG_C Cuts at: 2294 2330 2662 3791 4655 2294 Size: 36 332 1129 864 5002 Fragments arranged by size: 5002 1129 864 332 36 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7363 NotI GC'GGCC_GC Cuts at: 4810 4810 Size: 7363 NspI r_CATG'y Cuts at: 1132 1229 2761 4004 4121 4624 4721 6266 1132 Size: 97 1532 1243 117 503 97 1545 2229 Fragments arranged by size: 2229 1545 1532 1243 503 117 97 97 PasI CC'CwG_GG Cuts at: 1348 2556 1348 Size: 1208 6155 PciI A'CATG_T Cuts at: 2757 4620 6262 2757 Size: 1863 1642 3858 Fragments arranged by size: 3858 1863 1642 PflMI CCAn_nnn'nTGG Cuts at: 1108 2212 2875 3025 1108 Size: 1104 663 150 5446 Fragments arranged by size: 5446 1104 663 150 PmeI GTTT'AAAC Cuts at: 4667 4667 Size: 7363 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2841 2873 3185 3217 5432 5464 6945 6977 2841 Size: 32 312 32 2215 32 1481 32 3227 Fragments arranged by size: 3227 2215 1481 312 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2446 2906 4068 2134 Size: 312 460 1162 5429 Fragments arranged by size: 5429 1162 460 312 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7363 PsiI TTA'TAA Cuts at: 4933 4933 Size: 7363 PspOMI G'GGCC_C Cuts at: 2613 3816 2613 Size: 1203 6160 PstI C_TGCA'G Cuts at: 830 2817 3440 4227 4715 830 Size: 1987 623 787 488 3478 Fragments arranged by size: 3478 1987 787 623 488 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7363 PvuII CAG'CTG Cuts at: 2056 2302 3260 3441 4064 4222 4524 2056 Size: 246 958 181 623 158 302 4895 Fragments arranged by size: 4895 958 623 302 246 181 158 RsrII CG'GwC_CG Cuts at: 4597 4597 Size: 7363 SacI G_AGCT'C Cuts at: 2520 2520 Size: 7363 SacII CC_GC'GG Cuts at: 2106 2106 Size: 7363 SalI G'TCGA_C Cuts at: 1936 4705 1936 Size: 2769 4594 SanDI GG'GwC_CC Cuts at: 2906 2906 Size: 7363 SapI GCTCTTCn'nnn_ Cuts at: 2597 2597 Size: 7363 SbfI CC_TGCA'GG Cuts at: 4715 4715 Size: 7363 ScaI AGT'ACT Cuts at: 2564 5611 7323 2564 Size: 3047 1712 2604 Fragments arranged by size: 3047 2604 1712 SexAI A'CCwGG_T Cuts at: 2094 2094 Size: 7363 SfcI C'TryA_G Cuts at: 826 1045 2813 3436 4223 4642 4711 5846 6527 Size: 219 1768 623 787 419 69 1135 681 Cuts at: 6527 6718 826 Size: 191 1471 Fragments arranged by size: 1768 1471 1135 787 681 623 419 219 191 69 SfoI GGC'GCC Cuts at: 2422 2922 3840 4029 2422 Size: 500 918 189 5756 Fragments arranged by size: 5756 918 500 189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7363 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7363 SmaI CCC'GGG Cuts at: 1872 3762 4690 1872 Size: 1890 928 4545 Fragments arranged by size: 4545 1890 928 SmlI C'TyrA_G Cuts at: 820 1017 1943 3373 3389 5467 6368 6630 820 Size: 197 926 1430 16 2078 901 262 1553 Fragments arranged by size: 2078 1553 1430 926 901 262 197 16 SnaBI TAC'GTA Cuts at: 493 5154 493 Size: 4661 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7363 SphI G_CATG'C Cuts at: 1132 4004 4121 4721 1132 Size: 2872 117 600 3774 Fragments arranged by size: 3774 2872 600 117 SspI AAT'ATT Cuts at: 5 52 5287 5 Size: 47 5235 2081 Fragments arranged by size: 5235 2081 47 StuI AGG'CCT Cuts at: 1517 3149 4457 1517 Size: 1632 1308 4423 Fragments arranged by size: 4423 1632 1308 StyI C'CwwG_G Cuts at: 513 1065 2385 2535 3801 4350 4802 513 Size: 552 1320 150 1266 549 452 3074 Fragments arranged by size: 3074 1320 1266 552 549 452 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3261 3470 3614 4343 4432 4625 5390 Size: 1255 1681 209 144 729 89 193 765 Cuts at: 5390 5407 5560 5745 6190 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1681 1498 1255 765 729 445 209 193 185 153 144 89 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2562 5609 7321 Size: 274 80 33 51 1443 585 3047 1712 Cuts at: 7321 96 Size: 138 Fragments arranged by size: 3047 1712 1443 585 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7323 7355 7323 Size: 32 7331 Tth111I GACn'n_nGTC Cuts at: 1316 4381 1316 Size: 3065 4298 XbaI T'CTAG_A Cuts at: 4699 4699 Size: 7363 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2377 2377 Size: 7363 XhoI C'TCGA_G Cuts at: 1943 3389 1943 Size: 1446 5917 XmaI C'CCGG_G Cuts at: 1870 3760 4688 1870 Size: 1890 928 4545 Fragments arranged by size: 4545 1890 928 XmnI GAAnn'nnTTC Cuts at: 2012 4535 5492 2012 Size: 2523 957 3883 Fragments arranged by size: 3883 2523 957 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2497 5171 276 Size: 53 83 186 936 963 2674 2468 Fragments arranged by size: 2674 2468 963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI SfiI SrfI SwaI