(Circular) (Six-base) MAPSORT of: pFN21AA1628.seq Check: 3142 from: 1 to: 8455 pFN21AA1628 8455 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2271 3667 2271 Size: 1396 7059 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6265 278 Size: 53 83 186 936 4729 2468 Fragments arranged by size: 4729 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5798 1937 Size: 3861 4594 Acc65I G'GTAC_C Cuts at: 5776 5776 Size: 8455 AclI AA'CG_TT Cuts at: 1563 6582 6955 1563 Size: 5019 373 3063 Fragments arranged by size: 5019 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2579 2653 3761 4348 4705 5145 5178 5418 Size: 947 74 1108 587 357 440 33 240 Cuts at: 5418 6518 7902 8170 1632 Size: 1100 1384 268 1917 Fragments arranged by size: 1917 1384 1108 1100 947 587 440 357 268 240 74 33 AfeI AGC'GCT Cuts at: 3099 4523 3099 Size: 1424 7031 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8258 AflIII A'CryG_T Cuts at: 1203 7354 8331 1203 Size: 6151 977 1327 Fragments arranged by size: 6151 1327 977 AgeI A'CCGG_T Cuts at: 7286 7286 Size: 8455 AhdI GACnn_n'nnGTC Cuts at: 7184 7184 Size: 8455 AleI CACnn'nnGTG Cuts at: 1166 2210 2765 2768 4787 1166 Size: 1044 555 3 2019 4834 Fragments arranged by size: 4834 2019 1044 555 3 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8455 AlwNI CAG_nnn'CTG Cuts at: 2330 2602 2984 4371 5311 5326 6198 7770 2330 Size: 272 382 1387 940 15 872 1572 3015 Fragments arranged by size: 3015 1572 1387 940 872 382 272 15 ApaI G_GGCC'C Cuts at: 2150 3838 5281 2150 Size: 1688 1443 5324 Fragments arranged by size: 5324 1688 1443 ApaLI G'TGCA_C Cuts at: 3563 5069 5129 6512 7668 3563 Size: 1506 60 1383 1156 4350 Fragments arranged by size: 4350 1506 1383 1156 60 ApoI r'AATT_y Cuts at: 1493 5764 5990 6142 8251 1493 Size: 4271 226 152 2109 1697 Fragments arranged by size: 4271 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7009 160 Size: 872 5977 1606 Fragments arranged by size: 5977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8455 AvaI C'yCGr_G Cuts at: 1870 1943 3026 3359 4991 5274 5506 5579 5780 Size: 73 1083 333 1632 283 232 73 201 Cuts at: 5780 1870 Size: 4545 Fragments arranged by size: 4545 1632 1083 333 283 232 201 73 73 BamHI G'GATC_C Cuts at: 5518 5785 6152 5518 Size: 267 367 7821 Fragments arranged by size: 7821 367 267 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3022 3121 4216 4222 4608 Size: 325 217 627 1235 99 1095 6 386 Cuts at: 4608 5776 7231 618 Size: 1168 1455 1842 Fragments arranged by size: 1842 1455 1235 1168 1095 627 386 325 217 99 6 BanII G_rGCy'C Cuts at: 1391 2040 2150 2991 3838 4380 4577 4626 5020 Size: 649 110 841 847 542 197 49 394 Cuts at: 5020 5197 5281 5774 1391 Size: 177 84 493 4072 Fragments arranged by size: 4072 847 841 649 542 493 394 197 177 110 84 49 BbeI G_GCGC'C Cuts at: 3026 3125 4220 3026 Size: 99 1095 7261 Fragments arranged by size: 7261 1095 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4428 928 Size: 971 2529 4955 Fragments arranged by size: 4955 2529 971 BbvCI CC'TCA_GC Cuts at: 2806 4014 4728 2806 Size: 1208 714 6533 Fragments arranged by size: 6533 1208 714 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6646 6680 1903 Size: 34 4709 34 3678 Fragments arranged by size: 4709 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6347 7557 6347 Size: 1210 7245 BclI T'GATC_A Cuts at: 1548 4824 1548 Size: 3276 5179 BfrBI ATG'CAT Cuts at: 3853 3853 Size: 8455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3130 3139 4084 Size: 107 122 71 631 605 1458 9 945 Cuts at: 4084 4540 7066 136 Size: 456 2526 1525 Fragments arranged by size: 2526 1525 1458 945 631 605 456 122 107 71 9 BglII A'GATC_T Cuts at: 3975 5184 8450 3975 Size: 1209 3266 3980 Fragments arranged by size: 3980 3266 1209 BlpI GC'TnA_GC Cuts at: 2111 2985 5210 5872 2111 Size: 874 2225 662 4694 Fragments arranged by size: 4694 2225 874 662 Bme1580I G_kGCm'C Cuts at: 1790 2150 3567 3838 4613 5073 5133 5281 6516 Size: 360 1417 271 775 460 60 148 1235 Cuts at: 6516 7672 1790 Size: 1156 2573 Fragments arranged by size: 2573 1417 1235 1156 775 460 360 271 148 60 BmgBI CAC'GTC Cuts at: 1318 2734 4270 5110 1318 Size: 1416 1536 840 4663 Fragments arranged by size: 4663 1536 1416 840 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3579 3838 3861 Size: 416 53 83 399 24 2162 259 23 Cuts at: 3861 5310 6186 7144 442 Size: 1449 876 958 1753 Fragments arranged by size: 2162 1753 1449 958 876 416 399 259 83 53 24 23 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8455 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4770 4770 Size: 8455 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2239 2264 4245 4437 4763 4817 4996 5469 Size: 1000 25 1981 192 326 54 179 473 Cuts at: 5469 5483 5627 5694 7115 1239 Size: 14 144 67 1421 2579 Fragments arranged by size: 2579 1981 1421 1000 473 326 192 179 144 67 54 25 14 Bpu10I CC'TnA_GC Cuts at: 2806 3665 4014 4379 4680 4728 2806 Size: 859 349 365 301 48 6533 Fragments arranged by size: 6533 859 365 349 301 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4073 6580 7445 7743 4073 Size: 2507 865 298 4785 Fragments arranged by size: 4785 2507 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4050 5660 5746 7118 882 Size: 617 2551 1610 86 1372 2219 Fragments arranged by size: 2551 2219 1610 1372 617 86 BsaAI yAC'GTr Cuts at: 493 1204 3511 6246 493 Size: 711 2307 2735 2702 Fragments arranged by size: 2735 2702 2307 711 BsaBI GATnn'nnATC Cuts at: 8449 8449 Size: 8455 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 2589 2714 2928 Size: 53 83 186 936 486 570 125 214 Cuts at: 2928 3023 3122 3201 3863 4217 5337 6262 6644 Size: 95 99 79 662 354 1120 925 382 Cuts at: 6644 7279 275 Size: 635 1451 Fragments arranged by size: 1451 1120 936 925 662 635 570 486 382 354 214 186 125 99 95 83 79 53 BsaWI w'CCGG_w Cuts at: 6888 7286 7560 7707 8118 6888 Size: 398 274 147 411 7225 Fragments arranged by size: 7225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2069 2099 4529 4559 4829 Size: 30 165 30 666 30 2430 30 270 Cuts at: 4829 4859 1178 Size: 30 4774 Fragments arranged by size: 4774 2430 666 270 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2483 2573 4031 4034 4895 5678 5734 Size: 844 453 90 1458 3 861 783 56 Cuts at: 5734 5737 1186 Size: 3 3904 Fragments arranged by size: 3904 1458 861 844 783 453 90 56 3 3 BseYI C'CCAG_C Cuts at: 2292 3290 3525 3529 3553 3597 3659 4119 5033 Size: 998 235 4 24 44 62 460 914 Cuts at: 5033 5041 5214 5371 7658 8154 2292 Size: 8 173 157 2287 496 2593 Fragments arranged by size: 2593 2287 998 914 496 460 235 173 157 62 44 24 8 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2429 3095 3609 3938 4493 4586 1163 Size: 573 693 666 514 329 555 93 5032 Fragments arranged by size: 5032 693 666 573 555 514 329 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2026 2032 4036 5264 5905 6666 Size: 275 186 25 6 2004 1228 641 761 Cuts at: 6666 7694 1540 Size: 1028 2301 Fragments arranged by size: 2301 2004 1228 1028 761 641 275 186 25 6 BsiHKAI G_wGCw'C Cuts at: 2250 2991 3056 3567 4686 5020 5073 5133 6516 Size: 741 65 511 1119 334 53 60 1383 Cuts at: 6516 6601 7672 2250 Size: 85 1071 3033 Fragments arranged by size: 3033 1383 1119 1071 741 511 334 85 65 60 53 BsmI GAATG_Cn' Cuts at: 4390 5966 6059 4390 Size: 1576 93 6786 Fragments arranged by size: 6786 1576 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2090 2090 Size: 8455 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2150 2250 2991 3056 3567 3838 Size: 399 250 110 100 741 65 511 271 Cuts at: 3838 4380 4577 4613 4626 4686 5020 5073 5133 Size: 542 197 36 13 60 334 53 60 Cuts at: 5133 5197 5281 5774 6516 6601 7672 1391 Size: 64 84 493 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 741 542 511 493 399 334 271 250 197 110 100 85 84 65 64 60 60 53 36 13 BspEI T'CCGG_A Cuts at: 8118 8118 Size: 8455 BspHI T'CATG_A Cuts at: 6344 8074 6344 Size: 1730 6725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 3667 4572 5606 5810 844 Size: 1427 1396 905 1034 204 3489 Fragments arranged by size: 3489 1427 1396 1034 905 204 BsrBI CCG'CTC Cuts at: 1394 1823 3355 6342 1394 Size: 429 1532 2987 3507 Fragments arranged by size: 3507 2987 1532 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2549 3676 5356 6950 7124 65 Size: 1183 1301 1127 1680 1594 174 1396 Fragments arranged by size: 1680 1594 1396 1301 1183 1127 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 3203 3824 4134 4219 4539 5263 Size: 110 211 1182 621 310 85 320 724 Cuts at: 5263 7099 7286 1700 Size: 1836 187 2869 Fragments arranged by size: 2869 1836 1182 724 621 320 310 211 187 110 85 BsrGI T'GTAC_A Cuts at: 96 4656 96 Size: 4560 3895 BssHII G'CGCG_C Cuts at: 1925 2069 3059 4778 1925 Size: 144 990 1719 5602 Fragments arranged by size: 5602 1719 990 144 BssSI C'ACGA_G Cuts at: 4154 6515 7527 4154 Size: 2361 1012 5082 Fragments arranged by size: 5082 2361 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5618 794 Size: 4824 3631 BstBI TT'CG_AA Cuts at: 7259 7259 Size: 8455 BstEII G'GTnAC_C Cuts at: 1184 2212 4396 1184 Size: 1028 2184 5243 Fragments arranged by size: 5243 2184 1028 BstXI CCAn_nnnn'nTGG Cuts at: 3980 3980 Size: 8455 BstYI r'GATC_y Cuts at: 1972 2598 3975 5184 5518 5732 5785 6152 6537 Size: 626 1377 1209 334 214 53 367 385 Cuts at: 6537 6554 8006 8092 8104 8450 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1377 1209 626 385 367 346 334 214 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 2331 2556 5469 2331 Size: 225 2913 5317 Fragments arranged by size: 5317 2913 225 BtgI C'CryG_G Cuts at: 513 1065 2004 2026 2032 2374 3089 3267 4051 Size: 552 939 22 6 342 715 178 784 Cuts at: 4051 4590 4735 513 Size: 539 145 4233 Fragments arranged by size: 4233 939 784 715 552 539 342 178 145 22 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7716 Fragments arranged by size: 7716 683 56 BtsI GCAGTG_nn' Cuts at: 2129 2169 2320 2973 3309 3450 4197 4476 5971 Size: 40 151 653 336 141 747 279 1495 Cuts at: 5971 6765 6785 2129 Size: 794 20 3799 Fragments arranged by size: 3799 1495 794 747 653 336 279 151 141 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2806 2841 529 Size: 35 2242 35 6143 Fragments arranged by size: 6143 2242 35 35 DraI TTT'AAA Cuts at: 5759 5759 Size: 8455 DraIII CAC_nnn'GTG Cuts at: 4486 4486 Size: 8455 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2324 7462 809 Size: 507 1008 5138 1802 Fragments arranged by size: 5138 1802 1008 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2023 2029 2064 Size: 54 1425 50 275 18 193 6 35 Cuts at: 2064 2394 2643 2776 3092 3264 4132 5261 5902 Size: 330 249 133 316 172 868 1129 641 Cuts at: 5902 6791 8420 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1129 889 868 641 330 316 275 249 193 172 133 54 50 43 35 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2023 2029 5261 5902 1537 Size: 275 211 6 3232 641 4090 Fragments arranged by size: 4090 3232 641 275 211 6 EarI CTCTTCn'nnn_ Cuts at: 1353 6385 1353 Size: 5032 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7042 7416 7562 387 Size: 1064 138 5453 374 146 1280 Fragments arranged by size: 5453 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2989 5018 2989 Size: 2029 6426 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2239 2264 2579 2653 3761 4245 4348 Size: 393 607 25 315 74 1108 484 103 Cuts at: 4348 4437 4705 4763 4817 4996 5145 5178 5418 Size: 89 268 58 54 179 149 33 240 Cuts at: 5418 5469 5483 5627 5694 6518 7115 7902 8170 Size: 51 14 144 67 824 597 787 268 Cuts at: 8170 1239 Size: 1524 Fragments arranged by size: 1524 1108 824 787 607 597 484 393 315 268 268 240 179 149 144 103 89 74 67 58 54 51 33 25 14 EcoO109I rG'GnC_Cy Cuts at: 2627 2680 2706 3478 3834 3835 4076 4793 5255 Size: 53 26 772 356 1 241 717 462 Cuts at: 5255 5278 5473 2627 Size: 23 195 5609 Fragments arranged by size: 5609 772 717 462 356 241 195 53 26 23 1 EcoRI G'AATT_C Cuts at: 5764 6142 5764 Size: 378 8077 FspI TGC'GCA Cuts at: 6961 6961 Size: 8455 HaeII r_GCGC'y Cuts at: 1678 3026 3035 3101 3125 3297 4220 4525 7602 Size: 1348 9 66 24 172 923 305 3077 Cuts at: 7602 1678 Size: 2531 Fragments arranged by size: 3077 2531 1348 923 305 172 66 24 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3020 3052 4410 4442 4529 Size: 32 135 32 1448 32 1358 32 87 Cuts at: 4529 4561 4829 4850 4861 4882 5489 5521 5724 Size: 32 268 21 11 21 607 32 203 Cuts at: 5724 5756 7096 7128 7170 7202 1373 Size: 32 1340 32 42 32 2626 Fragments arranged by size: 2626 1448 1358 1340 607 268 203 135 87 42 32 32 32 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2049 4348 5799 6045 669 Size: 988 281 111 2299 1451 246 3079 Fragments arranged by size: 3079 2299 1451 988 281 246 111 HindIII A'AGCT_T Cuts at: 748 2417 748 Size: 1669 6786 HpaI GTT'AAC Cuts at: 6045 6045 Size: 8455 KasI G'GCGC_C Cuts at: 3022 3121 4216 3022 Size: 99 1095 7261 Fragments arranged by size: 7261 1095 99 KpnI G_GTAC'C Cuts at: 5780 5780 Size: 8455 MfeI C'AATT_G Cuts at: 6054 6054 Size: 8455 MluI A'CGCG_T Cuts at: 8331 8331 Size: 8455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2863 4584 7569 7753 1305 Size: 1558 1721 2985 184 2007 Fragments arranged by size: 2985 2007 1721 1558 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 2645 2778 10 Size: 54 1425 343 564 249 133 5687 Fragments arranged by size: 5687 1425 564 343 249 133 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2765 2768 3635 4176 4272 4787 Size: 648 1044 555 3 867 541 96 515 Cuts at: 4787 4883 6413 6772 6931 518 Size: 96 1530 359 159 2042 Fragments arranged by size: 2042 1530 1044 867 648 555 541 515 359 159 96 96 3 MspA1I CmG'CkG Cuts at: 1606 2028 2195 2637 2810 2984 3091 3127 3335 Size: 422 167 442 173 174 107 36 208 Cuts at: 3335 3529 5045 5871 6548 7696 7941 1606 Size: 194 1516 826 677 1148 245 2120 Fragments arranged by size: 2120 1516 1148 826 677 442 422 245 208 194 174 173 167 107 36 NaeI GCC'GGC Cuts at: 2023 3205 3826 4136 4221 5265 2023 Size: 1182 621 310 85 1044 5213 Fragments arranged by size: 5213 1182 1044 621 310 85 NarI GG'CG_CC Cuts at: 3023 3122 4217 3023 Size: 99 1095 7261 Fragments arranged by size: 7261 1095 99 NcoI C'CATG_G Cuts at: 513 1065 2004 4735 513 Size: 552 939 2731 4233 Fragments arranged by size: 4233 2731 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8455 NgoMIV G'CCGG_C Cuts at: 2021 3203 3824 4134 4219 5263 2021 Size: 1182 621 310 85 1044 5213 Fragments arranged by size: 5213 1182 1044 621 310 85 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8455 NotI GC'GGCC_GC Cuts at: 5902 5902 Size: 8455 NsiI A_TGCA'T Cuts at: 3855 3855 Size: 8455 NspI r_CATG'y Cuts at: 1132 1229 4234 4864 4927 5813 7358 1132 Size: 97 3005 630 63 886 1545 2229 Fragments arranged by size: 3005 2229 1545 886 630 97 63 PasI CC'CwG_GG Cuts at: 1348 4371 1348 Size: 3023 5432 PciI A'CATG_T Cuts at: 7354 7354 Size: 8455 PflMI CCAn_nnn'nTGG Cuts at: 1108 2785 3220 4741 5056 5461 1108 Size: 1677 435 1521 315 405 4102 Fragments arranged by size: 4102 1677 1521 435 405 315 PfoI T'CCnGG_A Cuts at: 2601 4833 2601 Size: 2232 6223 PmeI GTTT'AAAC Cuts at: 5759 5759 Size: 8455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6524 6556 8037 8069 6524 Size: 32 1481 32 6910 Fragments arranged by size: 6910 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2706 3478 4793 5473 2706 Size: 772 1315 680 5688 Fragments arranged by size: 5688 1315 772 680 PshAI GACnn'nnGTC Cuts at: 1613 2408 1613 Size: 795 7660 PsiI TTA'TAA Cuts at: 6025 6025 Size: 8455 PspOMI G'GGCC_C Cuts at: 2146 3834 5277 2146 Size: 1688 1443 5324 Fragments arranged by size: 5324 1688 1443 PstI C_TGCA'G Cuts at: 830 2164 2980 3198 3907 4818 5322 5381 5807 Size: 1334 816 218 709 911 504 59 426 Cuts at: 5807 830 Size: 3478 Fragments arranged by size: 3478 1334 911 816 709 504 426 218 59 PvuI CG_AT'CG Cuts at: 2001 4036 2001 Size: 2035 6420 PvuII CAG'CTG Cuts at: 2810 3529 5045 2810 Size: 719 1516 6220 Fragments arranged by size: 6220 1516 719 SacI G_AGCT'C Cuts at: 2991 5020 2991 Size: 2029 6426 SacII CC_GC'GG Cuts at: 2029 3092 2029 Size: 1063 7392 SalI G'TCGA_C Cuts at: 1936 5797 1936 Size: 3861 4594 SanDI GG'GwC_CC Cuts at: 2706 2706 Size: 8455 SbfI CC_TGCA'GG Cuts at: 3198 4818 5381 5807 3198 Size: 1620 563 426 5846 Fragments arranged by size: 5846 1620 563 426 ScaI AGT'ACT Cuts at: 6703 8415 6703 Size: 1712 6743 SexAI A'CCwGG_T Cuts at: 2541 5385 2541 Size: 2844 5611 SfcI C'TryA_G Cuts at: 826 1045 2160 2173 2976 3194 3467 3903 4814 Size: 219 1115 13 803 218 273 436 911 Cuts at: 4814 5318 5377 5803 6938 7619 7810 826 Size: 504 59 426 1135 681 191 1471 Fragments arranged by size: 1471 1135 1115 911 803 681 504 436 426 273 219 218 191 59 13 SfoI GGC'GCC Cuts at: 3024 3123 4218 3024 Size: 99 1095 7261 Fragments arranged by size: 7261 1095 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8455 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8455 SmaI CCC'GGG Cuts at: 1872 4993 5276 5581 5782 1872 Size: 3121 283 305 201 4545 Fragments arranged by size: 4545 3121 305 283 201 SmlI C'TyrA_G Cuts at: 820 1017 1943 4088 6559 7460 7722 820 Size: 197 926 2145 2471 901 262 1553 Fragments arranged by size: 2471 2145 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6246 493 Size: 5753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8455 SphI G_CATG'C Cuts at: 1132 5813 1132 Size: 4681 3774 SspI AAT'ATT Cuts at: 5 52 6379 5 Size: 47 6327 2081 Fragments arranged by size: 6327 2081 47 StuI AGG'CCT Cuts at: 1517 4971 5724 1517 Size: 3454 753 4248 Fragments arranged by size: 4248 3454 753 StyI C'CwwG_G Cuts at: 513 1065 2004 4735 5894 513 Size: 552 939 2731 1159 3074 Fragments arranged by size: 3074 2731 1159 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2320 3986 4877 5132 6482 6499 6652 Size: 1255 740 1666 891 255 1350 17 153 Cuts at: 6652 6837 7282 325 Size: 185 445 1498 Fragments arranged by size: 1666 1498 1350 1255 891 740 445 255 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3687 4656 5007 Size: 274 80 33 51 1443 1710 969 351 Cuts at: 5007 6701 8413 96 Size: 1694 1712 138 Fragments arranged by size: 1712 1710 1694 1443 969 351 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2690 2722 4943 4975 8415 8447 2690 Size: 32 2221 32 3440 32 2698 Fragments arranged by size: 3440 2698 2221 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2735 4585 1316 Size: 1419 1850 5186 Fragments arranged by size: 5186 1850 1419 XbaI T'CTAG_A Cuts at: 5791 5791 Size: 8455 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3102 3161 3556 4127 3102 Size: 59 395 571 7430 Fragments arranged by size: 7430 571 395 59 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8455 XmaI C'CCGG_G Cuts at: 1870 4991 5274 5579 5780 1870 Size: 3121 283 305 201 4545 Fragments arranged by size: 4545 3121 305 283 201 XmnI GAAnn'nnTTC Cuts at: 4448 6584 4448 Size: 2136 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6263 276 Size: 53 83 186 936 4729 2468 Fragments arranged by size: 4729 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SapI SfiI SrfI SwaI