(Circular) (Six-base) MAPSORT of: pFN21AB5238.seq Check: 8700 from: 1 to: 5326 pFN21AB5238 5326 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2544 3136 278 Size: 53 83 186 936 1008 592 2468 Fragments arranged by size: 2468 1008 936 592 186 83 53 AccI GT'mk_AC Cuts at: 1937 2669 1937 Size: 732 4594 Acc65I G'GTAC_C Cuts at: 2647 2647 Size: 5326 AclI AA'CG_TT Cuts at: 1563 3453 3826 1563 Size: 1890 373 3063 Fragments arranged by size: 3063 1890 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2221 2222 2420 3389 4773 5041 1632 Size: 589 1 198 969 1384 268 1917 Fragments arranged by size: 1917 1384 969 589 268 198 1 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5129 AflIII A'CryG_T Cuts at: 1203 4225 5202 1203 Size: 3022 977 1327 Fragments arranged by size: 3022 1327 977 AgeI A'CCGG_T Cuts at: 4157 4157 Size: 5326 AhdI GACnn_n'nnGTC Cuts at: 4055 4055 Size: 5326 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5326 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5326 AlwNI CAG_nnn'CTG Cuts at: 3069 4641 3069 Size: 1572 3754 ApaLI G'TGCA_C Cuts at: 2248 3383 4539 2248 Size: 1135 1156 3035 Fragments arranged by size: 3035 1156 1135 ApoI r'AATT_y Cuts at: 1493 2635 2861 3013 5122 1493 Size: 1142 226 152 2109 1697 Fragments arranged by size: 2109 1697 1142 226 152 AseI AT'TA_AT Cuts at: 160 1032 3880 160 Size: 872 2848 1606 Fragments arranged by size: 2848 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 AvaI C'yCGr_G Cuts at: 1870 1943 2651 1870 Size: 73 708 4545 Fragments arranged by size: 4545 708 73 BamHI G'GATC_C Cuts at: 2656 3023 2656 Size: 367 4959 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2647 4102 618 Size: 325 217 627 860 1455 1842 Fragments arranged by size: 1842 1455 860 627 325 217 BanII G_rGCy'C Cuts at: 1391 2105 2245 2645 1391 Size: 714 140 400 4072 Fragments arranged by size: 4072 714 400 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2524 2558 3517 3551 1903 Size: 34 50 34 503 34 959 34 3678 Fragments arranged by size: 3678 959 503 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3218 4428 3218 Size: 1210 4116 BclI T'GATC_A Cuts at: 1548 1548 Size: 5326 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2322 3937 Size: 107 122 71 631 605 337 313 1615 Cuts at: 3937 136 Size: 1525 Fragments arranged by size: 1615 1525 631 605 337 313 122 107 71 BglII A'GATC_T Cuts at: 5321 5321 Size: 5326 BlpI GC'TnA_GC Cuts at: 2743 2743 Size: 5326 Bme1580I G_kGCm'C Cuts at: 1790 2252 3387 4543 1790 Size: 462 1135 1156 2573 Fragments arranged by size: 2573 1156 1135 462 BmgBI CAC'GTC Cuts at: 1318 2158 2524 1318 Size: 840 366 4120 Fragments arranged by size: 4120 840 366 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2455 3057 4015 Size: 416 53 83 399 24 1038 602 958 Cuts at: 4015 442 Size: 1753 Fragments arranged by size: 1753 1038 958 602 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5326 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 3986 1239 Size: 977 1770 2579 Fragments arranged by size: 2579 1770 977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3451 4316 4614 3451 Size: 865 298 4163 Fragments arranged by size: 4163 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3989 882 Size: 617 2490 2219 Fragments arranged by size: 2490 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2407 3117 493 Size: 711 1203 710 2702 Fragments arranged by size: 2702 1203 711 710 BsaBI GATnn'nnATC Cuts at: 2600 5320 2600 Size: 2720 2606 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2163 2541 3133 3515 Size: 53 83 186 936 630 378 592 382 Cuts at: 3515 4150 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 630 592 382 378 186 83 53 BsaWI w'CCGG_w Cuts at: 3759 4157 4431 4578 4989 3759 Size: 398 274 147 411 4096 Fragments arranged by size: 4096 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 1178 Size: 30 165 30 861 30 4210 Fragments arranged by size: 4210 861 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2116 2119 1186 Size: 930 3 4393 Fragments arranged by size: 4393 930 3 BseYI C'CCAG_C Cuts at: 4529 5025 4529 Size: 496 4830 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2458 1163 Size: 573 722 4031 Fragments arranged by size: 4031 722 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2185 2776 3537 4565 1540 Size: 275 186 184 591 761 1028 2301 Fragments arranged by size: 2301 1028 761 591 275 186 184 BsiHKAI G_wGCw'C Cuts at: 2252 3387 3472 4543 2252 Size: 1135 85 1071 3035 Fragments arranged by size: 3035 1135 1071 85 BsmI GAATG_Cn' Cuts at: 2837 2930 2837 Size: 93 5233 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2245 2252 2645 3387 3472 4543 Size: 399 315 140 7 393 742 85 1071 Cuts at: 4543 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 393 315 140 85 7 BspEI T'CCGG_A Cuts at: 4989 4989 Size: 5326 BspHI T'CATG_A Cuts at: 3215 4945 3215 Size: 1730 3596 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2681 844 Size: 1837 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2143 2179 3213 1394 Size: 429 320 36 1034 3507 Fragments arranged by size: 3507 1034 429 320 36 BsrDI GCAATG_nn' Cuts at: 65 1248 3821 3995 65 Size: 1183 2573 174 1396 Fragments arranged by size: 2573 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3970 4157 1700 Size: 110 2160 187 2869 Fragments arranged by size: 2869 2160 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5326 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5326 BssSI C'ACGA_G Cuts at: 2144 3386 4398 2144 Size: 1242 1012 3072 Fragments arranged by size: 3072 1242 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5326 BstBI TT'CG_AA Cuts at: 4130 4130 Size: 5326 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5326 BstYI r'GATC_y Cuts at: 1972 2656 3023 3408 3425 4877 4963 4975 5321 Size: 684 367 385 17 1452 86 12 346 Cuts at: 5321 1972 Size: 1977 Fragments arranged by size: 1977 1452 684 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2079 2427 513 Size: 552 1014 348 3412 Fragments arranged by size: 3412 1014 552 348 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4587 Fragments arranged by size: 4587 683 56 BtsI GCAGTG_nn' Cuts at: 2842 3636 3656 2842 Size: 794 20 4512 Fragments arranged by size: 4512 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5326 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5291 DraI TTT'AAA Cuts at: 2630 2630 Size: 5326 DraIII CAC_nnn'GTG Cuts at: 2296 2296 Size: 5326 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4333 809 Size: 507 3017 1802 Fragments arranged by size: 3017 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2182 2614 2773 Size: 54 1425 50 275 18 352 432 159 Cuts at: 2773 3662 5291 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 432 352 275 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2182 2773 1537 Size: 275 370 591 4090 Fragments arranged by size: 4090 591 370 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2125 3256 1353 Size: 772 1131 3423 Fragments arranged by size: 3423 1131 772 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3913 4287 4433 387 Size: 1064 138 2324 374 146 1280 Fragments arranged by size: 2324 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2221 2222 2420 3389 3986 4773 Size: 393 584 5 1 198 969 597 787 Cuts at: 4773 5041 1239 Size: 268 1524 Fragments arranged by size: 1524 969 787 597 584 393 268 198 5 1 EcoNI CCTnn'n_nnAGG Cuts at: 2290 2290 Size: 5326 EcoRI G'AATT_C Cuts at: 2635 3013 2635 Size: 378 4948 FspI TGC'GCA Cuts at: 3832 3832 Size: 5326 HaeII r_GCGC'y Cuts at: 1678 2015 4473 1678 Size: 337 2458 2531 Fragments arranged by size: 2531 2458 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 3967 3999 4041 Size: 32 135 32 530 32 1833 32 42 Cuts at: 4041 4073 1373 Size: 32 2626 Fragments arranged by size: 2626 1833 530 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2670 2916 669 Size: 988 281 732 246 3079 Fragments arranged by size: 3079 988 732 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5326 HpaI GTT'AAC Cuts at: 2916 2916 Size: 5326 KpnI G_GTAC'C Cuts at: 2651 2651 Size: 5326 MfeI C'AATT_G Cuts at: 2925 2925 Size: 5326 MluI A'CGCG_T Cuts at: 5202 5202 Size: 5326 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2475 4440 4624 1305 Size: 1170 1965 184 2007 Fragments arranged by size: 2007 1965 1170 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2616 10 Size: 54 1425 343 784 2720 Fragments arranged by size: 2720 1425 784 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3284 3643 3802 518 Size: 648 2118 359 159 2042 Fragments arranged by size: 2118 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2031 2068 2081 2228 2742 3419 4567 4812 Size: 425 37 13 147 514 677 1148 245 Cuts at: 4812 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 514 425 245 147 37 13 NcoI C'CATG_G Cuts at: 513 1065 2427 513 Size: 552 1362 3412 Fragments arranged by size: 3412 1362 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5326 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5326 NotI GC'GGCC_GC Cuts at: 2773 2773 Size: 5326 NspI r_CATG'y Cuts at: 1132 1229 2510 2684 4229 1132 Size: 97 1281 174 1545 2229 Fragments arranged by size: 2229 1545 1281 174 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5326 PciI A'CATG_T Cuts at: 4225 4225 Size: 5326 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5326 PfoI T'CCnGG_A Cuts at: 2132 2132 Size: 5326 PmeI GTTT'AAAC Cuts at: 2630 2630 Size: 5326 PmlI CAC'GTG Cuts at: 2407 2407 Size: 5326 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3395 3427 4908 4940 3395 Size: 32 1481 32 3781 Fragments arranged by size: 3781 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5326 PsiI TTA'TAA Cuts at: 2896 2896 Size: 5326 PstI C_TGCA'G Cuts at: 830 2096 2678 830 Size: 1266 582 3478 Fragments arranged by size: 3478 1266 582 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5326 PvuII CAG'CTG Cuts at: 2228 2228 Size: 5326 SacII CC_GC'GG Cuts at: 2082 2082 Size: 5326 SalI G'TCGA_C Cuts at: 1936 2668 1936 Size: 732 4594 SbfI CC_TGCA'GG Cuts at: 2678 2678 Size: 5326 ScaI AGT'ACT Cuts at: 3574 5286 3574 Size: 1712 3614 SfcI C'TryA_G Cuts at: 826 1045 2092 2674 3809 4490 4681 826 Size: 219 1047 582 1135 681 191 1471 Fragments arranged by size: 1471 1135 1047 681 582 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5326 SmaI CCC'GGG Cuts at: 1872 2653 1872 Size: 781 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3430 4331 4593 820 Size: 197 926 1487 901 262 1553 Fragments arranged by size: 1553 1487 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3117 493 Size: 2624 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5326 SphI G_CATG'C Cuts at: 1132 2684 1132 Size: 1552 3774 SspI AAT'ATT Cuts at: 5 52 3250 5 Size: 47 3198 2081 Fragments arranged by size: 3198 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5326 StyI C'CwwG_G Cuts at: 513 1065 2427 2765 513 Size: 552 1362 338 3074 Fragments arranged by size: 3074 1362 552 338 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2507 3353 3370 3523 3708 4153 325 Size: 1255 927 846 17 153 185 445 1498 Fragments arranged by size: 1498 1255 927 846 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3572 5284 96 Size: 274 80 33 51 1443 1595 1712 138 Fragments arranged by size: 1712 1595 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5286 5318 5286 Size: 32 5294 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5326 XbaI T'CTAG_A Cuts at: 2662 2662 Size: 5326 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5326 XmaI C'CCGG_G Cuts at: 1870 2651 1870 Size: 781 4545 XmnI GAAnn'nnTTC Cuts at: 2264 3455 2264 Size: 1191 4135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2542 3134 276 Size: 53 83 186 936 1008 592 2468 Fragments arranged by size: 2468 1008 936 592 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI