Restriction map for pFN21AB5238
 (Circular) (Six-base) MAPSORT of: pFN21AB5238.seq  Check: 8700  from: 1  to: 5326



pFN21AB5238  5326 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 11:00  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2544    3136     278 
   Size:         53      83     186     936    1008     592    2468

  Fragments arranged by size: 

                2468    1008     936     592     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2669    1937 
   Size:        732    4594
 
Acc65I G'GTAC_C

Cuts at:   2647    2647 
   Size:       5326
 
AclI AA'CG_TT

Cuts at:   1563    3453    3826    1563 
   Size:       1890     373    3063

  Fragments arranged by size: 

                3063    1890     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2221    2222    2420    3389    4773    5041    1632 
   Size:        589       1     198     969    1384     268    1917

  Fragments arranged by size: 

                1917    1384     969     589     268     198       1
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5129
 
AflIII A'CryG_T

Cuts at:   1203    4225    5202    1203 
   Size:       3022     977    1327

  Fragments arranged by size: 

                3022    1327     977
 
AgeI A'CCGG_T

Cuts at:   4157    4157 
   Size:       5326
 
AhdI GACnn_n'nnGTC

Cuts at:   4055    4055 
   Size:       5326
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5326
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5326
 
AlwNI CAG_nnn'CTG

Cuts at:   3069    4641    3069 
   Size:       1572    3754
 
ApaLI G'TGCA_C

Cuts at:   2248    3383    4539    2248 
   Size:       1135    1156    3035

  Fragments arranged by size: 

                3035    1156    1135
 
ApoI r'AATT_y

Cuts at:   1493    2635    2861    3013    5122    1493 
   Size:       1142     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1142     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3880     160 
   Size:        872    2848    1606

  Fragments arranged by size: 

                2848    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5326
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2651    1870 
   Size:         73     708    4545

  Fragments arranged by size: 

                4545     708      73
 
BamHI G'GATC_C

Cuts at:   2656    3023    2656 
   Size:        367    4959
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2647    4102     618 
   Size:        325     217     627     860    1455    1842

  Fragments arranged by size: 

                1842    1455     860     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2105    2245    2645    1391 
   Size:        714     140     400    4072

  Fragments arranged by size: 

                4072     714     400     140
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4355
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    2524    2558    3517    3551    1903 
   Size:         34      50      34     503      34     959      34    3678

  Fragments arranged by size: 

                3678     959     503      50      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   3218    4428    3218 
   Size:       1210    4116
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5326
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2322    3937 
   Size:        107     122      71     631     605     337     313    1615

Cuts at:   3937     136 
   Size:       1525

  Fragments arranged by size: 

                1615    1525     631     605     337     313     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5321    5321 
   Size:       5326
 
BlpI GC'TnA_GC

Cuts at:   2743    2743 
   Size:       5326
 
Bme1580I G_kGCm'C

Cuts at:   1790    2252    3387    4543    1790 
   Size:        462    1135    1156    2573

  Fragments arranged by size: 

                2573    1156    1135     462
 
BmgBI CAC'GTC

Cuts at:   1318    2158    2524    1318 
   Size:        840     366    4120

  Fragments arranged by size: 

                4120     840     366
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2455    3057    4015 
   Size:        416      53      83     399      24    1038     602     958

Cuts at:   4015     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1038     958     602     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5326
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2216    3986    1239 
   Size:        977    1770    2579

  Fragments arranged by size: 

                2579    1770     977
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3451    4316    4614    3451 
   Size:        865     298    4163

  Fragments arranged by size: 

                4163     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3989     882 
   Size:        617    2490    2219

  Fragments arranged by size: 

                2490    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2407    3117     493 
   Size:        711    1203     710    2702

  Fragments arranged by size: 

                2702    1203     711     710
 
BsaBI GATnn'nnATC

Cuts at:   2600    5320    2600 
   Size:       2720    2606
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2163    2541    3133    3515 
   Size:         53      83     186     936     630     378     592     382

Cuts at:   3515    4150     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     936     635     630     592     382     378     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   3759    4157    4431    4578    4989    3759 
   Size:        398     274     147     411    4096

  Fragments arranged by size: 

                4096     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2264    2294    1178 
   Size:         30     165      30     861      30    4210

  Fragments arranged by size: 

                4210     861     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2116    2119    1186 
   Size:        930       3    4393

  Fragments arranged by size: 

                4393     930       3
 
BseYI C'CCAG_C

Cuts at:   4529    5025    4529 
   Size:        496    4830
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2458    1163 
   Size:        573     722    4031

  Fragments arranged by size: 

                4031     722     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2185    2776    3537    4565    1540 
   Size:        275     186     184     591     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     591     275     186     184
 
BsiHKAI G_wGCw'C

Cuts at:   2252    3387    3472    4543    2252 
   Size:       1135      85    1071    3035

  Fragments arranged by size: 

                3035    1135    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2837    2930    2837 
   Size:         93    5233
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2105    2245    2252    2645    3387    3472    4543 
   Size:        399     315     140       7     393     742      85    1071

Cuts at:   4543    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     399     393     315     140      85
                   7
 
BspEI T'CCGG_A

Cuts at:   4989    4989 
   Size:       5326
 
BspHI T'CATG_A

Cuts at:   3215    4945    3215 
   Size:       1730    3596
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2681     844 
   Size:       1837    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2143    2179    3213    1394 
   Size:        429     320      36    1034    3507

  Fragments arranged by size: 

                3507    1034     429     320      36
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3821    3995      65 
   Size:       1183    2573     174    1396

  Fragments arranged by size: 

                2573    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3970    4157    1700 
   Size:        110    2160     187    2869

  Fragments arranged by size: 

                2869    2160     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5326
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5326
 
BssSI C'ACGA_G

Cuts at:   2144    3386    4398    2144 
   Size:       1242    1012    3072

  Fragments arranged by size: 

                3072    1242    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5326
 
BstBI TT'CG_AA

Cuts at:   4130    4130 
   Size:       5326
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5326
 
BstYI r'GATC_y

Cuts at:   1972    2656    3023    3408    3425    4877    4963    4975    5321 
   Size:        684     367     385      17    1452      86      12     346

Cuts at:   5321    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     684     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2079    2427     513 
   Size:        552    1014     348    3412

  Fragments arranged by size: 

                3412    1014     552     348
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4587

  Fragments arranged by size: 

                4587     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2842    3636    3656    2842 
   Size:        794      20    4512

  Fragments arranged by size: 

                4512     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5326
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5291
 
DraI TTT'AAA

Cuts at:   2630    2630 
   Size:       5326
 
DraIII CAC_nnn'GTG

Cuts at:   2296    2296 
   Size:       5326
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4333     809 
   Size:        507    3017    1802

  Fragments arranged by size: 

                3017    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2182    2614    2773 
   Size:         54    1425      50     275      18     352     432     159

Cuts at:   2773    3662    5291       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     432     352     275     159      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2182    2773    1537 
   Size:        275     370     591    4090

  Fragments arranged by size: 

                4090     591     370     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2125    3256    1353 
   Size:        772    1131    3423

  Fragments arranged by size: 

                3423    1131     772
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3913    4287    4433     387 
   Size:       1064     138    2324     374     146    1280

  Fragments arranged by size: 

                2324    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2216    2221    2222    2420    3389    3986    4773 
   Size:        393     584       5       1     198     969     597     787

Cuts at:   4773    5041    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     969     787     597     584     393     268     198
                   5       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2290    2290 
   Size:       5326
 
EcoRI G'AATT_C

Cuts at:   2635    3013    2635 
   Size:        378    4948
 
FspI TGC'GCA

Cuts at:   3832    3832 
   Size:       5326
 
HaeII r_GCGC'y

Cuts at:   1678    2015    4473    1678 
   Size:        337    2458    2531

  Fragments arranged by size: 

                2531    2458     337
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2102    2134    3967    3999    4041 
   Size:         32     135      32     530      32    1833      32      42

Cuts at:   4041    4073    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1833     530     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2670    2916     669 
   Size:        988     281     732     246    3079

  Fragments arranged by size: 

                3079     988     732     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5326
 
HpaI GTT'AAC

Cuts at:   2916    2916 
   Size:       5326
 
KpnI G_GTAC'C

Cuts at:   2651    2651 
   Size:       5326
 
MfeI C'AATT_G

Cuts at:   2925    2925 
   Size:       5326
 
MluI A'CGCG_T

Cuts at:   5202    5202 
   Size:       5326
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2475    4440    4624    1305 
   Size:       1170    1965     184    2007

  Fragments arranged by size: 

                2007    1965    1170     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2616      10 
   Size:         54    1425     343     784    2720

  Fragments arranged by size: 

                2720    1425     784     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3284    3643    3802     518 
   Size:        648    2118     359     159    2042

  Fragments arranged by size: 

                2118    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2031    2068    2081    2228    2742    3419    4567    4812 
   Size:        425      37      13     147     514     677    1148     245

Cuts at:   4812    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     514     425     245     147      37
                  13
 
NcoI C'CATG_G

Cuts at:    513    1065    2427     513 
   Size:        552    1362    3412

  Fragments arranged by size: 

                3412    1362     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5326
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5326
 
NotI GC'GGCC_GC

Cuts at:   2773    2773 
   Size:       5326
 
NspI r_CATG'y

Cuts at:   1132    1229    2510    2684    4229    1132 
   Size:         97    1281     174    1545    2229

  Fragments arranged by size: 

                2229    1545    1281     174      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5326
 
PciI A'CATG_T

Cuts at:   4225    4225 
   Size:       5326
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5326
 
PfoI T'CCnGG_A

Cuts at:   2132    2132 
   Size:       5326
 
PmeI GTTT'AAAC

Cuts at:   2630    2630 
   Size:       5326
 
PmlI CAC'GTG

Cuts at:   2407    2407 
   Size:       5326
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3395    3427    4908    4940    3395 
   Size:         32    1481      32    3781

  Fragments arranged by size: 

                3781    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5326
 
PsiI TTA'TAA

Cuts at:   2896    2896 
   Size:       5326
 
PstI C_TGCA'G

Cuts at:    830    2096    2678     830 
   Size:       1266     582    3478

  Fragments arranged by size: 

                3478    1266     582
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5326
 
PvuII CAG'CTG

Cuts at:   2228    2228 
   Size:       5326
 
SacII CC_GC'GG

Cuts at:   2082    2082 
   Size:       5326
 
SalI G'TCGA_C

Cuts at:   1936    2668    1936 
   Size:        732    4594
 
SbfI CC_TGCA'GG

Cuts at:   2678    2678 
   Size:       5326
 
ScaI AGT'ACT

Cuts at:   3574    5286    3574 
   Size:       1712    3614
 
SfcI C'TryA_G

Cuts at:    826    1045    2092    2674    3809    4490    4681     826 
   Size:        219    1047     582    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1047     681     582     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5326
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5326
 
SmaI CCC'GGG

Cuts at:   1872    2653    1872 
   Size:        781    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3430    4331    4593     820 
   Size:        197     926    1487     901     262    1553

  Fragments arranged by size: 

                1553    1487     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3117     493 
   Size:       2624    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5326
 
SphI G_CATG'C

Cuts at:   1132    2684    1132 
   Size:       1552    3774
 
SspI AAT'ATT

Cuts at:      5      52    3250       5 
   Size:         47    3198    2081

  Fragments arranged by size: 

                3198    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5326
 
StyI C'CwwG_G

Cuts at:    513    1065    2427    2765     513 
   Size:        552    1362     338    3074

  Fragments arranged by size: 

                3074    1362     552     338
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2507    3353    3370    3523    3708    4153     325 
   Size:       1255     927     846      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     927     846     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3572    5284      96 
   Size:        274      80      33      51    1443    1595    1712     138

  Fragments arranged by size: 

                1712    1595    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5286    5318    5286 
   Size:         32    5294
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5326
 
XbaI T'CTAG_A

Cuts at:   2662    2662 
   Size:       5326
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5326
 
XmaI C'CCGG_G

Cuts at:   1870    2651    1870 
   Size:        781    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2264    3455    2264 
   Size:       1191    4135
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2542    3134     276 
   Size:         53      83     186     936    1008     592    2468

  Fragments arranged by size: 

                2468    1008     936     592     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII
    SacII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I  EcoICRI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI    PpuMI   PspOMI
    PspXI     PsrI    RsrII     SacI    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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