(Circular) (Six-base) MAPSORT of: pFN21AB5236.seq Check: 6387 from: 1 to: 5767 pFN21AB5236 5767 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2014 3045 3110 1937 Size: 77 1031 65 4594 Fragments arranged by size: 4594 1031 77 65 Acc65I G'GTAC_C Cuts at: 3088 3088 Size: 5767 AclI AA'CG_TT Cuts at: 1563 3066 3894 4267 1563 Size: 1503 828 373 3063 Fragments arranged by size: 3063 1503 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2366 3037 3830 5214 5482 1632 Size: 734 671 793 1384 268 1917 Fragments arranged by size: 1917 1384 793 734 671 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 4666 5643 1203 Size: 3463 977 1327 Fragments arranged by size: 3463 1327 977 AgeI A'CCGG_T Cuts at: 4598 4598 Size: 5767 AhdI GACnn_n'nnGTC Cuts at: 4496 4496 Size: 5767 AleI CACnn'nnGTG Cuts at: 1166 2163 1166 Size: 997 4770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5767 AlwNI CAG_nnn'CTG Cuts at: 3510 5082 3510 Size: 1572 4195 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 2189 2895 3076 3302 3454 5563 1493 Size: 696 706 181 226 152 2109 1697 Fragments arranged by size: 2109 1697 706 696 226 181 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 3092 1870 Size: 73 1149 4545 Fragments arranged by size: 4545 1149 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2439 2472 2439 Size: 33 5734 BamHI G'GATC_C Cuts at: 3097 3464 3097 Size: 367 5400 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3088 4543 618 Size: 325 217 627 1301 1455 1842 Fragments arranged by size: 1842 1455 1301 627 325 217 BanII G_rGCy'C Cuts at: 1391 2679 3086 1391 Size: 1288 407 4072 Fragments arranged by size: 4072 1288 407 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4796 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3958 3992 1903 Size: 34 2021 34 3678 Fragments arranged by size: 3678 2021 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2294 3659 4869 2294 Size: 1365 1210 3192 Fragments arranged by size: 3192 1365 1210 BclI T'GATC_A Cuts at: 1548 2050 1548 Size: 502 5265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2971 4378 136 Size: 107 122 71 631 605 1299 1407 1525 Fragments arranged by size: 1525 1407 1299 631 605 122 107 71 BglII A'GATC_T Cuts at: 5762 5762 Size: 5767 BlpI GC'TnA_GC Cuts at: 3184 3184 Size: 5767 Bme1580I G_kGCm'C Cuts at: 1790 2703 3828 4984 1790 Size: 913 1125 1156 2573 Fragments arranged by size: 2573 1156 1125 913 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2513 3498 4456 Size: 416 53 83 399 24 1096 985 958 Cuts at: 4456 442 Size: 1753 Fragments arranged by size: 1753 1096 985 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4427 1239 Size: 3188 2579 Bpu10I CC'TnA_GC Cuts at: 2218 2218 Size: 5767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2255 3892 4757 5055 2255 Size: 1637 865 298 2967 Fragments arranged by size: 2967 1637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2193 4430 882 Size: 617 694 2237 2219 Fragments arranged by size: 2237 2219 694 617 BsaAI yAC'GTr Cuts at: 493 1204 3558 493 Size: 711 2354 2702 Fragments arranged by size: 2702 2354 711 BsaBI GATnn'nnATC Cuts at: 2036 5761 2036 Size: 3725 2042 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2592 3574 3956 4591 Size: 53 83 186 936 1059 982 382 635 Cuts at: 4591 275 Size: 1451 Fragments arranged by size: 1451 1059 982 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4200 4598 4872 5019 5430 4200 Size: 398 274 147 411 4537 Fragments arranged by size: 4537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5542 Fragments arranged by size: 5542 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5767 BseYI C'CCAG_C Cuts at: 2043 4970 5466 2043 Size: 2927 496 2344 Fragments arranged by size: 2927 2344 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5194 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3217 3978 5006 1540 Size: 275 186 1216 761 1028 2301 Fragments arranged by size: 2301 1216 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2857 3828 3913 4984 2857 Size: 971 85 1071 3640 Fragments arranged by size: 3640 1071 971 85 BsmI GAATG_Cn' Cuts at: 3278 3371 3278 Size: 93 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2679 2703 2857 3086 3828 3913 4984 Size: 399 889 24 154 229 742 85 1071 Cuts at: 4984 1391 Size: 2174 Fragments arranged by size: 2174 1071 889 742 399 229 154 85 24 BspEI T'CCGG_A Cuts at: 5430 5430 Size: 5767 BspHI T'CATG_A Cuts at: 2080 3656 5386 2080 Size: 1576 1730 2461 Fragments arranged by size: 2461 1730 1576 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3122 844 Size: 2278 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3654 1394 Size: 429 1831 3507 Fragments arranged by size: 3507 1831 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2176 2626 4262 4436 65 Size: 1183 928 450 1636 174 1396 Fragments arranged by size: 1636 1396 1183 928 450 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2624 4411 4598 1700 Size: 110 814 1787 187 2869 Fragments arranged by size: 2869 1787 814 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 2528 3827 4839 2528 Size: 1299 1012 3456 Fragments arranged by size: 3456 1299 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5767 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstYI r'GATC_y Cuts at: 1972 3097 3464 3849 3866 5318 5404 5416 5762 Size: 1125 367 385 17 1452 86 12 346 Cuts at: 5762 1972 Size: 1977 Fragments arranged by size: 1977 1452 1125 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2295 513 Size: 552 1230 3985 Fragments arranged by size: 3985 1230 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5028 Fragments arranged by size: 5028 683 56 BtsI GCAGTG_nn' Cuts at: 2177 2362 3003 3283 4077 4097 2177 Size: 185 641 280 794 20 3847 Fragments arranged by size: 3847 794 641 280 185 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4774 809 Size: 507 3458 1802 Fragments arranged by size: 3458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2779 3214 4103 Size: 54 1425 50 275 18 949 435 889 Cuts at: 4103 5732 8 Size: 1629 43 Fragments arranged by size: 1629 1425 949 889 435 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3214 1537 Size: 275 1402 4090 Fragments arranged by size: 4090 1402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2003 3697 1353 Size: 650 1694 3423 Fragments arranged by size: 3423 1694 650 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2948 4354 4728 4874 387 Size: 1064 138 1359 1406 374 146 1280 Fragments arranged by size: 1406 1359 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 3037 3830 4427 5214 5482 1239 Size: 393 734 671 793 597 787 268 1524 Fragments arranged by size: 1524 793 787 734 671 597 393 268 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 2512 2977 4914 1678 Size: 834 465 1937 2531 Fragments arranged by size: 2531 1937 834 465 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4408 4440 4482 4514 1373 Size: 32 135 32 2836 32 42 32 2626 Fragments arranged by size: 2836 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 2835 748 Size: 2087 3680 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KpnI G_GTAC'C Cuts at: 3092 3092 Size: 5767 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4881 5065 1305 Size: 3576 184 2007 Fragments arranged by size: 3576 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3945 Fragments arranged by size: 3945 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2163 2182 3725 4084 4243 518 Size: 648 997 19 1543 359 159 2042 Fragments arranged by size: 2042 1543 997 648 359 159 19 MspA1I CmG'CkG Cuts at: 1606 2584 2913 3183 3860 5008 5253 1606 Size: 978 329 270 677 1148 245 2120 Fragments arranged by size: 2120 1148 978 677 329 270 245 NaeI GCC'GGC Cuts at: 2626 2626 Size: 5767 NcoI C'CATG_G Cuts at: 513 1065 2295 513 Size: 552 1230 3985 Fragments arranged by size: 3985 1230 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NgoMIV G'CCGG_C Cuts at: 2624 2624 Size: 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 3214 3214 Size: 5767 NspI r_CATG'y Cuts at: 1132 1229 3125 4670 1132 Size: 97 1896 1545 2229 Fragments arranged by size: 2229 1896 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5767 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5767 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3836 3868 5349 5381 3836 Size: 32 1481 32 4222 Fragments arranged by size: 4222 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 3337 3337 Size: 5767 PstI C_TGCA'G Cuts at: 830 2981 2998 3119 830 Size: 2151 17 121 3478 Fragments arranged by size: 3478 2151 121 17 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 PvuII CAG'CTG Cuts at: 2584 2584 Size: 5767 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SfcI C'TryA_G Cuts at: 826 1045 2205 2977 2994 3115 4250 4931 5122 Size: 219 1160 772 17 121 1135 681 191 Cuts at: 5122 826 Size: 1471 Fragments arranged by size: 1471 1160 1135 772 681 219 191 121 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 3094 1872 Size: 1222 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2270 3871 4772 5034 820 Size: 197 926 327 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 327 262 197 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5767 StyI C'CwwG_G Cuts at: 513 1065 2145 2295 3206 513 Size: 552 1080 150 911 3074 Fragments arranged by size: 3074 1080 911 552 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3794 3811 3964 4149 4594 325 Size: 1255 2214 17 153 185 445 1498 Fragments arranged by size: 2214 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4013 5725 96 Size: 274 80 33 51 1443 2036 1712 138 Fragments arranged by size: 2036 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5727 5759 5727 Size: 32 5735 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5767 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2108 2108 Size: 5767 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5767 XmaI C'CCGG_G Cuts at: 1870 3092 1870 Size: 1222 4545 XmnI GAAnn'nnTTC Cuts at: 3896 3896 Size: 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI