(Circular) (Six-base) MAPSORT of: pFN21AB5210.seq Check: 9694 from: 1 to: 5689 pFN21AB5210 5689 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2287 2287 Size: 5689 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2085 2397 3032 1937 Size: 148 312 635 4594 Fragments arranged by size: 4594 635 312 148 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2480 2723 2754 3752 5136 5404 1632 Size: 848 243 31 998 1384 268 1917 Fragments arranged by size: 1917 1384 998 848 268 243 31 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 2258 1166 Size: 1092 4597 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2190 1184 Size: 1006 4683 AlwNI CAG_nnn'CTG Cuts at: 2542 2701 3432 5004 2542 Size: 159 731 1572 3227 Fragments arranged by size: 3227 1572 731 159 ApaLI G'TGCA_C Cuts at: 2175 3746 4902 2175 Size: 1571 1156 2962 Fragments arranged by size: 2962 1571 1156 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2386 3014 1870 Size: 73 443 628 4545 Fragments arranged by size: 4545 628 443 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2263 2312 2594 3010 Size: 325 217 627 288 188 49 282 416 Cuts at: 3010 4465 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 416 325 288 282 217 188 49 BanII G_rGCy'C Cuts at: 1391 2695 2808 3008 1391 Size: 1304 113 200 4072 Fragments arranged by size: 4072 1304 200 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2039 2687 928 Size: 971 140 648 3930 Fragments arranged by size: 3930 971 648 140 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2816 4300 136 Size: 107 122 71 631 605 1144 1484 1525 Fragments arranged by size: 1525 1484 1144 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2107 2145 2179 2268 2599 3750 4906 1790 Size: 317 38 34 89 331 1151 1156 2573 Fragments arranged by size: 2573 1156 1151 331 317 89 38 34 BmgBI CAC'GTC Cuts at: 1318 2566 1318 Size: 1248 4441 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2034 2885 4349 1239 Size: 795 851 1464 2579 Fragments arranged by size: 2579 1464 851 795 Bpu10I CC'TnA_GC Cuts at: 2694 2694 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2075 2285 2936 3814 4679 4977 2075 Size: 210 651 878 865 298 2787 Fragments arranged by size: 2787 878 865 651 298 210 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2029 2845 3480 493 Size: 711 825 816 635 2702 Fragments arranged by size: 2702 825 816 711 635 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2244 3496 3878 4513 Size: 53 83 186 936 711 1252 382 635 Cuts at: 4513 275 Size: 1451 Fragments arranged by size: 1451 1252 936 711 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2456 2486 1178 Size: 30 165 30 1053 30 4381 Fragments arranged by size: 4381 1053 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2182 1186 Size: 996 4693 BseYI C'CCAG_C Cuts at: 2106 2267 2556 2937 2962 4892 5388 2106 Size: 161 289 381 25 1930 496 2407 Fragments arranged by size: 2407 1930 496 381 289 161 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2009 1163 Size: 573 273 4843 Fragments arranged by size: 4843 573 273 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2179 3750 3835 4906 2179 Size: 1571 85 1071 2962 Fragments arranged by size: 2962 1571 1071 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2145 2179 2268 2599 2695 2808 Size: 399 317 38 34 89 331 96 113 Cuts at: 2808 3008 3750 3835 4906 1391 Size: 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 331 317 200 113 96 89 85 38 34 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2287 3044 844 Size: 1443 757 3489 Fragments arranged by size: 3489 1443 757 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 4333 4520 1700 Size: 110 262 2261 187 2869 Fragments arranged by size: 2869 2261 262 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 2897 3749 4761 2897 Size: 852 1012 3825 Fragments arranged by size: 3825 1012 852 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstXI CCAn_nnnn'nTGG Cuts at: 2260 2671 2260 Size: 411 5278 BstYI r'GATC_y Cuts at: 1972 2127 2793 3019 3386 3771 3788 5240 5326 Size: 155 666 226 367 385 17 1452 86 Cuts at: 5326 5338 5684 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 666 385 367 346 226 155 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2580 513 Size: 552 1515 3622 Fragments arranged by size: 3622 1515 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2476 2560 487 Size: 683 56 1250 84 3616 Fragments arranged by size: 3616 1250 683 84 56 BtsI GCAGTG_nn' Cuts at: 3205 3999 4019 3205 Size: 794 20 4875 Fragments arranged by size: 4875 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2570 4696 809 Size: 507 1254 2126 1802 Fragments arranged by size: 2126 1802 1254 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2229 2361 Size: 54 1425 50 275 18 240 159 132 Cuts at: 2361 2760 2826 3136 4025 5654 8 Size: 399 66 310 889 1629 43 Fragments arranged by size: 1629 1425 889 399 310 275 240 159 132 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3619 1353 Size: 2266 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2480 2723 2754 2885 3752 4349 Size: 393 402 446 243 31 131 867 597 Cuts at: 4349 5136 5404 1239 Size: 787 268 1524 Fragments arranged by size: 1524 867 787 597 446 402 393 268 243 131 31 EcoO109I rG'GnC_Cy Cuts at: 2368 2515 2668 2698 2368 Size: 147 153 30 5359 Fragments arranged by size: 5359 153 147 30 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2299 2951 4836 1678 Size: 621 652 1885 2531 Fragments arranged by size: 2531 1885 652 621 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4330 4362 4404 4436 1373 Size: 32 135 32 2758 32 42 32 2626 Fragments arranged by size: 2758 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2884 4803 4987 1305 Size: 1579 1919 184 2007 Fragments arranged by size: 2007 1919 1579 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 10 Size: 54 1425 343 531 3336 Fragments arranged by size: 3336 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2258 2261 2579 3647 4006 4165 518 Size: 648 1092 3 318 1068 359 159 2042 Fragments arranged by size: 2042 1092 1068 648 359 318 159 3 MspA1I CmG'CkG Cuts at: 1606 2831 2887 2941 2962 3105 3782 4930 5175 Size: 1225 56 54 21 143 677 1148 245 Cuts at: 5175 1606 Size: 2120 Fragments arranged by size: 2120 1225 1148 677 245 143 56 54 21 NaeI GCC'GGC Cuts at: 2074 2074 Size: 5689 NcoI C'CATG_G Cuts at: 513 1065 2580 513 Size: 552 1515 3622 Fragments arranged by size: 3622 1515 552 NdeI CA'TA_TG Cuts at: 387 2224 387 Size: 1837 3852 NgoMIV G'CCGG_C Cuts at: 2072 2072 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 2671 1348 Size: 1323 4366 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PmlI CAC'GTG Cuts at: 2029 2845 2029 Size: 816 4873 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PstI C_TGCA'G Cuts at: 830 2547 3041 830 Size: 1717 494 3478 Fragments arranged by size: 3478 1717 494 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2941 2962 2941 Size: 21 5668 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 2543 2836 3037 4172 4853 5044 826 Size: 219 1498 293 201 1135 681 191 1471 Fragments arranged by size: 1498 1471 1135 681 293 219 201 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 2388 3016 1872 Size: 516 628 4545 Fragments arranged by size: 4545 628 516 SmlI C'TyrA_G Cuts at: 820 1017 1943 2090 2300 2951 3793 4694 4956 Size: 197 926 147 210 651 842 901 262 Cuts at: 4956 820 Size: 1553 Fragments arranged by size: 1553 926 901 842 651 262 210 197 147 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2364 2580 2664 2985 3128 513 Size: 552 1299 216 84 321 143 3074 Fragments arranged by size: 3074 1299 552 321 216 143 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 3716 3733 3886 4071 4516 325 Size: 1255 741 1395 17 153 185 445 1498 Fragments arranged by size: 1498 1395 1255 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3935 5647 96 Size: 274 80 33 51 1443 1958 1712 138 Fragments arranged by size: 1958 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2456 2488 5649 5681 2456 Size: 32 3161 32 2464 Fragments arranged by size: 3161 2464 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2323 2323 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 2386 3014 1870 Size: 516 628 4545 Fragments arranged by size: 4545 628 516 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI