Restriction map for
pFN21AB5167
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbrPI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MaeI
MamI MluI MroI MslI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RcaI RsrII SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AlwNI 922 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 679 GGGCCC GGGCC^C
BbeI 1504 GGCGCC GGCGC^C
BbvCI 1377 CCTCAGC CCTCAGC(-5/-2)
BcgI 618 CGACGTGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1232 CCCGG CC^[GC]GG
BpmI 1462 CTGGAG CTGGAG(16/14)
BsmBI 1468 GAGACG CGTCTC(1/5)
BspLU11I 800 ACATGT A^CATGT
BsrBI 1545 GAGCGG CCGCTC(-3/-3)
BsrI 338 CCAGT ACTGG(1/-1)
BstAPI 1320 GCATGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 459 GGATCT [GA]^GATC[CT]
DrdI 1363 GACCGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 50 CGGCCG C^GGCCG
EclXI 50 CGGCCG C^GGCCG
Eco52I 50 CGGCCG C^GGCCG
HgaI 1006 GACGC GACGC(5/10)
KasI 1504 GGCGCC G^GCGCC
KspI 33 CCGCGG CCGC^GG
MfeI 562 CAATTG C^AATTG
MflI 459 GGATCT [GA]^GATC[CT]
MunI 562 CAATTG C^AATTG
NarI 1504 GGCGCC GG^CGCC
NciI 1232 CCCGG CC^[GC]GG
NcoI 905 CCATGG C^CATGG
PshAI 1046 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 286 GAGCTC GAGCT^C
SacII 33 CCGCGG CCGC^GG
SalI 1519 GTCGAC G^TCGAC
SfoI 1504 GGCGCC GGC^GCC
SspI 251 AATATT AAT^ATT
StuI 1424 AGGCCT AGG^CCT
TfiI 915 GAATC G^A[AT]TC
Tsp509I 563 AATT ^AATT
XcmI 1298 CCAACGCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 459 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 295 GTCTAC GT^[AC][GT]AC
1519 GTCGAC
AcyI 1006 GACGCC G[GA]^CG[CT]C
1504 GGCGCC
AfaI 593 GTAC GT^AC
996 GTAC
AflIII 620 ACGTGT A^C[GA][CT]GT
800 ACATGT
AlwI 567 GATCC GGATC(4/5)
459 GGATC
Aor51HI 80 AGCGCT AGC^GCT
111 AGCGCT
BalI 479 TGGCCA TGG^CCA
902 TGGCCA
BglI 968 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1303 GCCATGCTGGC
BsaHI 1006 GACGCC G[GA]^CG[CT]C
1504 GGCGCC
BsiEI 50 CGGCCG CG[GA][CT]^CG
1362 CGACCG
BsmAI 558 GAGAC GTCTC(1/5)
1468 GAGAC
BstEII 1185 GGTCACC G^GT[ACGT]ACC
1371 GGTCACC
BstPI 1185 GGTCACC G^GT[ACGT]ACC
1371 GGTCACC
Eco47III 80 AGCGCT AGC^GCT
111 AGCGCT
EcoNI 182 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1254 CCTTGAGGAGG
EcoO65I 1185 GGTCACC G^GT[ACGT]ACC
1371 GGTCACC
Hin1I 1006 GACGCC G[GA]^CG[CT]C
1504 GGCGCC
HincII 328 GTTGAC GT[CT]^[GA]AC
1519 GTCGAC
HindII 328 GTTGAC GT[CT]^[GA]AC
1519 GTCGAC
MluNI 479 TGGCCA TGG^CCA
902 TGGCCA
MscI 479 TGGCCA TGG^CCA
902 TGGCCA
NspI 800 ACATGT [GA]CATG^[CT]
1346 GCATGT
PpuMI 467 GGGTCCT [GA]G^G[AT]CC[CT]
1044 GGGACCC
RsaI 593 GTAC GT^AC
996 GTAC
SfaNI 139 GATGC GCATC(5/9)
728 GCATC
SmlI 383 CTCAAG C^T[CT][GA]AG
1255 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanI BanII BbsI BbvI BmyI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PvuII
Sau3AI Sau96I ScrFI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I