(Circular) (Six-base) MAPSORT of: pFN21AB5167.seq Check: 8456 from: 1 to: 6265 pFN21AB5167 6265 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3193 3193 Size: 6265 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2301 3525 3608 1937 Size: 364 1224 83 4594 Fragments arranged by size: 4594 1224 364 83 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2837 3065 4328 5712 5980 1632 Size: 1205 228 1263 1384 268 1917 Fragments arranged by size: 1917 1384 1263 1205 268 228 AfeI AGC'GCT Cuts at: 2087 2118 2087 Size: 31 6234 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 2625 2805 5164 6141 1203 Size: 1422 180 2359 977 1327 Fragments arranged by size: 2359 1422 1327 977 180 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 2932 4008 5580 2932 Size: 1076 1572 3617 Fragments arranged by size: 3617 1572 1076 ApaI G_GGCC'C Cuts at: 2688 2688 Size: 6265 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 3590 1870 Size: 73 1647 4545 Fragments arranged by size: 4545 1647 73 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3134 3182 3509 3586 5041 Size: 325 217 627 1347 48 327 77 1455 Cuts at: 5041 618 Size: 1842 Fragments arranged by size: 1842 1455 1347 627 327 325 217 77 48 BanII G_rGCy'C Cuts at: 1391 2295 2688 3099 3584 1391 Size: 904 393 411 485 4072 Fragments arranged by size: 4072 904 485 411 393 BbeI G_GCGC'C Cuts at: 3513 3513 Size: 6265 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2057 2112 2844 3258 3540 928 Size: 971 158 55 732 414 282 3653 Fragments arranged by size: 3653 971 732 414 282 158 55 BbvCI CC'TCA_GC Cuts at: 3383 3383 Size: 6265 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2612 2646 4456 4490 1903 Size: 34 675 34 1810 34 3678 Fragments arranged by size: 3678 1810 675 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2979 3314 4876 Size: 107 122 71 631 605 1307 335 1562 Cuts at: 4876 136 Size: 1525 Fragments arranged by size: 1562 1525 1307 631 605 335 122 107 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 2180 2688 2792 4326 5482 1790 Size: 390 508 104 1534 1156 2573 Fragments arranged by size: 2573 1534 1156 508 390 104 BmgBI CAC'GTC Cuts at: 1318 2626 2779 1318 Size: 1308 153 4804 Fragments arranged by size: 4804 1308 153 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2337 3996 4954 Size: 416 53 83 399 24 920 1659 958 Cuts at: 4954 442 Size: 1753 Fragments arranged by size: 1753 1659 958 920 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2446 3496 2446 Size: 1050 5215 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3488 4925 1239 Size: 2249 1437 2579 Fragments arranged by size: 2579 2249 1437 Bpu10I CC'TnA_GC Cuts at: 3383 3383 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2373 3281 4390 5255 5553 2373 Size: 908 1109 865 298 3085 Fragments arranged by size: 3085 1109 908 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3012 3510 4072 4454 Size: 53 83 186 936 1479 498 562 382 Cuts at: 4454 5089 275 Size: 635 1451 Fragments arranged by size: 1479 1451 936 635 562 498 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2960 2990 1178 Size: 30 165 30 1557 30 4453 Fragments arranged by size: 4453 1557 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2978 2981 3107 3278 3485 3512 3560 Size: 928 864 3 126 171 207 27 48 Cuts at: 3560 1186 Size: 3891 Fragments arranged by size: 3891 928 864 207 171 126 48 27 3 BseYI C'CCAG_C Cuts at: 5468 5964 5468 Size: 496 5769 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3170 3377 3392 1163 Size: 573 1434 207 15 4036 Fragments arranged by size: 4036 1434 573 207 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2058 3370 3715 4476 5504 1540 Size: 275 186 57 1312 345 761 1028 2301 Fragments arranged by size: 2301 1312 1028 761 345 275 186 57 BsiHKAI G_wGCw'C Cuts at: 2295 2368 3210 3336 4326 4411 5482 2295 Size: 73 842 126 990 85 1071 3078 Fragments arranged by size: 3078 1071 990 842 126 85 73 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 BsmBI CGTCTCn'nnnn_ Cuts at: 3467 3467 Size: 6265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 2295 2368 2688 2792 3099 3210 Size: 399 390 115 73 320 104 307 111 Cuts at: 3210 3336 3584 4326 4411 5482 1391 Size: 126 248 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 320 307 248 126 115 111 104 85 73 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3193 3399 3414 3620 844 Size: 2349 206 15 206 3489 Fragments arranged by size: 3489 2349 206 206 15 BsrBI CCG'CTC Cuts at: 1394 1823 3552 4152 1394 Size: 429 1729 600 3507 Fragments arranged by size: 3507 1729 600 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3115 3269 3362 4909 5096 1700 Size: 110 1305 154 93 1547 187 2869 Fragments arranged by size: 2869 1547 1305 187 154 110 93 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 3331 794 Size: 2537 3728 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 3190 3376 1184 Size: 2006 186 4073 Fragments arranged by size: 4073 2006 186 BstYI r'GATC_y Cuts at: 1972 2464 3595 3962 4347 4364 5816 5902 5914 Size: 492 1131 367 385 17 1452 86 12 Cuts at: 5914 6260 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1131 492 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2038 2888 2904 2910 3186 513 Size: 552 939 34 850 16 6 276 3592 Fragments arranged by size: 3592 939 850 552 276 34 16 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 3394 3781 4575 4595 3394 Size: 387 794 20 5064 Fragments arranged by size: 5064 794 387 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3374 5272 809 Size: 507 2058 1898 1802 Fragments arranged by size: 2058 1898 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2266 2484 Size: 54 1425 50 275 18 225 211 218 Cuts at: 2484 2907 3171 3712 4601 6230 8 Size: 423 264 541 889 1629 43 Fragments arranged by size: 1629 1425 889 541 423 275 264 225 218 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2055 3712 1537 Size: 275 243 1657 4090 Fragments arranged by size: 4090 1657 275 243 EarI CTCTTCn'nnn_ Cuts at: 1353 2555 2827 2955 3480 4195 1353 Size: 1202 272 128 525 715 3423 Fragments arranged by size: 3423 1202 715 525 272 128 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2412 4852 5226 5372 387 Size: 1064 138 823 2440 374 146 1280 Fragments arranged by size: 2440 1280 1064 823 374 146 138 EcoICRI GAG'CTC Cuts at: 2293 2293 Size: 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2837 3065 3488 4328 4925 5712 5980 Size: 393 1205 228 423 840 597 787 268 Cuts at: 5980 1239 Size: 1524 Fragments arranged by size: 1524 1205 840 787 597 423 393 268 228 EcoNI CCTnn'n_nnAGG Cuts at: 2191 3263 2191 Size: 1072 5193 EcoO109I rG'GnC_Cy Cuts at: 2473 2684 3050 3143 2473 Size: 211 366 93 5595 Fragments arranged by size: 5595 366 211 93 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3275 3275 Size: 6265 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2089 2120 2362 3513 5412 1678 Size: 411 31 242 1151 1899 2531 Fragments arranged by size: 2531 1899 1151 411 242 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2527 2556 2559 2588 2960 Size: 32 135 32 955 29 3 29 372 Cuts at: 2960 2992 4906 4938 4980 5012 1373 Size: 32 1914 32 42 32 2626 Fragments arranged by size: 2626 1914 955 372 135 42 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 2335 3526 3609 3855 669 Size: 988 281 397 1191 83 246 3079 Fragments arranged by size: 3079 1191 988 397 281 246 83 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KasI G'GCGC_C Cuts at: 3509 3509 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 2567 3864 2567 Size: 1297 4968 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2513 3327 5379 5563 1305 Size: 1208 814 2052 184 2007 Fragments arranged by size: 2052 2007 1208 814 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2486 2909 10 Size: 54 1425 343 654 423 3366 Fragments arranged by size: 3366 1425 654 423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4223 4582 4741 518 Size: 648 3057 359 159 2042 Fragments arranged by size: 3057 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2040 2404 3043 3154 3540 3681 4358 5506 Size: 434 364 639 111 386 141 677 1148 Cuts at: 5506 5751 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 639 434 386 364 245 141 111 NaeI GCC'GGC Cuts at: 3117 3271 3364 3117 Size: 154 93 6018 Fragments arranged by size: 6018 154 93 NarI GG'CG_CC Cuts at: 3510 3510 Size: 6265 NcoI C'CATG_G Cuts at: 513 1065 2004 2910 513 Size: 552 939 906 3868 Fragments arranged by size: 3868 939 906 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NgoMIV G'CCGG_C Cuts at: 3115 3269 3362 3115 Size: 154 93 6018 Fragments arranged by size: 6018 154 93 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2809 3355 3623 5168 1132 Size: 97 1580 546 268 1545 2229 Fragments arranged by size: 2229 1580 1545 546 268 97 PasI CC'CwG_GG Cuts at: 1348 2725 3139 1348 Size: 1377 414 4474 Fragments arranged by size: 4474 1377 414 PciI A'CATG_T Cuts at: 2805 5164 2805 Size: 2359 3906 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2496 2894 1108 Size: 1269 119 398 4479 Fragments arranged by size: 4479 1269 398 119 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2473 3050 2473 Size: 577 5688 PshAI GACnn'nnGTC Cuts at: 1613 3055 1613 Size: 1442 4823 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspOMI G'GGCC_C Cuts at: 2684 2684 Size: 6265 PstI C_TGCA'G Cuts at: 830 3617 830 Size: 2787 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2404 3043 3154 2404 Size: 639 111 5515 Fragments arranged by size: 5515 639 111 SacI G_AGCT'C Cuts at: 2295 2295 Size: 6265 SacII CC_GC'GG Cuts at: 2041 2041 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3524 3607 1936 Size: 1588 83 4594 Fragments arranged by size: 4594 1588 83 SanDI GG'GwC_CC Cuts at: 3050 3050 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SfcI C'TryA_G Cuts at: 826 1045 3613 4748 5429 5620 826 Size: 219 2568 1135 681 191 1471 Fragments arranged by size: 2568 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3511 3511 Size: 6265 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2388 3260 4369 5270 5532 820 Size: 197 926 445 872 1109 901 262 1553 Fragments arranged by size: 1553 1109 926 901 872 445 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 2258 4189 5 Size: 47 2206 1931 2081 Fragments arranged by size: 2206 2081 1931 47 StuI AGG'CCT Cuts at: 1517 3431 1517 Size: 1914 4351 StyI C'CwwG_G Cuts at: 513 1065 2004 2192 2910 3432 3704 513 Size: 552 939 188 718 522 272 3074 Fragments arranged by size: 3074 939 718 552 522 272 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2707 3152 4292 4309 4462 4647 5092 Size: 1255 1127 445 1140 17 153 185 445 Cuts at: 5092 325 Size: 1498 Fragments arranged by size: 1498 1255 1140 1127 445 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2597 3000 4511 Size: 274 80 33 51 1443 620 403 1511 Cuts at: 4511 6223 96 Size: 1712 138 Fragments arranged by size: 1712 1511 1443 620 403 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2159 2191 6225 6257 2159 Size: 32 4034 32 2167 Fragments arranged by size: 4034 2167 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3310 3310 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI