Restriction map for
pFN21AB5167
(Linear) (Six-base) MAPSORT of: KIBB5167.seq Check: 7046 from: 1 to: 1560
KIBB5167.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1188 1560
Size: 1188 372
AccI GT'mk_AC
Cuts at: 0 296 1520 1560
Size: 296 1224 40
Fragments arranged by size:
1224 296 40
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1060 1560
Size: 832 228 500
Fragments arranged by size:
832 500 228
AfeI AGC'GCT
Cuts at: 0 82 113 1560
Size: 82 31 1447
Fragments arranged by size:
1447 82 31
AflIII A'CryG_T
Cuts at: 0 620 800 1560
Size: 620 180 760
Fragments arranged by size:
760 620 180
AlwNI CAG_nnn'CTG
Cuts at: 0 927 1560
Size: 927 633
ApaI G_GGCC'C
Cuts at: 0 683 1560
Size: 683 877
BanI G'GyrC_C
Cuts at: 0 1129 1177 1504 1560
Size: 1129 48 327 56
Fragments arranged by size:
1129 327 56 48
BanII G_rGCy'C
Cuts at: 0 290 683 1094 1560
Size: 290 393 411 466
Fragments arranged by size:
466 411 393 290
BbeI G_GCGC'C
Cuts at: 0 1508 1560
Size: 1508 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 52 107 839 1253 1535 1560
Size: 52 55 732 414 282 25
Fragments arranged by size:
732 414 282 55 52 25
BbvCI CC'TCA_GC
Cuts at: 0 1378 1560
Size: 1378 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 607 641 1560
Size: 607 34 919
Fragments arranged by size:
919 607 34
BglI GCCn_nnn'nGGC
Cuts at: 0 974 1309 1560
Size: 974 335 251
Fragments arranged by size:
974 335 251
Bme1580I G_kGCm'C
Cuts at: 0 175 683 787 1560
Size: 175 508 104 773
Fragments arranged by size:
773 508 175 104
BmgBI CAC'GTC
Cuts at: 0 621 774 1560
Size: 621 153 786
Fragments arranged by size:
786 621 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 332 1560
Size: 332 1228
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 441 1491 1560
Size: 441 1050 69
Fragments arranged by size:
1050 441 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1483 1560
Size: 1483 77
Bpu10I CC'TnA_GC
Cuts at: 0 1378 1560
Size: 1378 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 1276 1560
Size: 368 908 284
Fragments arranged by size:
908 368 284
BsaHI Gr'CG_yC
Cuts at: 0 1007 1505 1560
Size: 1007 498 55
Fragments arranged by size:
1007 498 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 955 985 1560
Size: 955 30 575
Fragments arranged by size:
955 575 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 973 976 1102 1273 1480 1507 1555
Size: 109 864 3 126 171 207 27 48
Cuts at: 1555 1560
Size: 5
Fragments arranged by size:
864 207 171 126 109 48 27 5
3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1372 1387 1560
Size: 1165 207 15 173
Fragments arranged by size:
1165 207 173 15
BsiEI CG_ry'CG
Cuts at: 0 53 1365 1560
Size: 53 1312 195
Fragments arranged by size:
1312 195 53
BsiHKAI G_wGCw'C
Cuts at: 0 290 363 1205 1331 1560
Size: 290 73 842 126 229
Fragments arranged by size:
842 290 229 126 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1462 1560
Size: 1462 98
Bsp1286I G_dGCh'C
Cuts at: 0 175 290 363 683 787 1094 1205 1331
Size: 175 115 73 320 104 307 111 126
Cuts at: 1331 1560
Size: 229
Fragments arranged by size:
320 307 229 175 126 115 111 104
73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1188 1394 1409 1560
Size: 1188 206 15 151
Fragments arranged by size:
1188 206 151 15
BsrBI CCG'CTC
Cuts at: 0 1547 1560
Size: 1547 13
BsrFI r'CCGG_y
Cuts at: 0 1110 1264 1357 1560
Size: 1110 154 93 203
Fragments arranged by size:
1110 203 154 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1326 1560
Size: 1326 234
BstEII G'GTnAC_C
Cuts at: 0 1185 1371 1560
Size: 1185 186 189
Fragments arranged by size:
1185 189 186
BstYI r'GATC_y
Cuts at: 0 459 1560
Size: 459 1101
BtgI C'CryG_G
Cuts at: 0 33 883 899 905 1181 1560
Size: 33 850 16 6 276 379
Fragments arranged by size:
850 379 276 33 16 6
BtsI GCAGTG_nn'
Cuts at: 0 1389 1560
Size: 1389 171
DrdI GACnn_nn'nnGTC
Cuts at: 0 1369 1560
Size: 1369 191
EaeI y'GGCC_r
Cuts at: 0 50 261 479 902 1166 1560
Size: 50 211 218 423 264 394
Fragments arranged by size:
423 394 264 218 211 50
EagI C'GGCC_G
Cuts at: 0 50 1560
Size: 50 1510
EarI CTCTTCn'nnn_
Cuts at: 0 550 822 950 1475 1560
Size: 550 272 128 525 85
Fragments arranged by size:
550 525 272 128 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 407 1560
Size: 407 1153
EcoICRI GAG'CTC
Cuts at: 0 288 1560
Size: 288 1272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1060 1483 1560
Size: 832 228 423 77
Fragments arranged by size:
832 423 228 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 186 1258 1560
Size: 186 1072 302
Fragments arranged by size:
1072 302 186
EcoO109I rG'GnC_Cy
Cuts at: 0 468 679 1045 1138 1560
Size: 468 211 366 93 422
Fragments arranged by size:
468 422 366 211 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1270 1560
Size: 1270 290
HaeII r_GCGC'y
Cuts at: 0 84 115 357 1508 1560
Size: 84 31 242 1151 52
Fragments arranged by size:
1151 242 84 52 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 522 551 554 583 955 987 1560
Size: 522 29 3 29 372 32 573
Fragments arranged by size:
573 522 372 32 29 29 3
HincII GTy'rAC
Cuts at: 0 330 1521 1560
Size: 330 1191 39
Fragments arranged by size:
1191 330 39
KasI G'GCGC_C
Cuts at: 0 1504 1560
Size: 1504 56
MfeI C'AATT_G
Cuts at: 0 562 1560
Size: 562 998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1322 1560
Size: 508 814 238
Fragments arranged by size:
814 508 238
MscI TGG'CCA
Cuts at: 0 481 904 1560
Size: 481 423 656
Fragments arranged by size:
656 481 423
MspA1I CmG'CkG
Cuts at: 0 35 399 1038 1149 1535 1560
Size: 35 364 639 111 386 25
Fragments arranged by size:
639 386 364 111 35 25
NaeI GCC'GGC
Cuts at: 0 1112 1266 1359 1560
Size: 1112 154 93 201
Fragments arranged by size:
1112 201 154 93
NarI GG'CG_CC
Cuts at: 0 1505 1560
Size: 1505 55
NcoI C'CATG_G
Cuts at: 0 905 1560
Size: 905 655
NgoMIV G'CCGG_C
Cuts at: 0 1110 1264 1357 1560
Size: 1110 154 93 203
Fragments arranged by size:
1110 203 154 93
NspI r_CATG'y
Cuts at: 0 804 1350 1560
Size: 804 546 210
Fragments arranged by size:
804 546 210
PasI CC'CwG_GG
Cuts at: 0 720 1134 1560
Size: 720 414 426
Fragments arranged by size:
720 426 414
PciI A'CATG_T
Cuts at: 0 800 1560
Size: 800 760
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 491 889 1560
Size: 372 119 398 671
Fragments arranged by size:
671 398 372 119
PpuMI rG'GwC_Cy
Cuts at: 0 468 1045 1560
Size: 468 577 515
Fragments arranged by size:
577 515 468
PshAI GACnn'nnGTC
Cuts at: 0 1050 1560
Size: 1050 510
PspOMI G'GGCC_C
Cuts at: 0 679 1560
Size: 679 881
PvuII CAG'CTG
Cuts at: 0 399 1038 1149 1560
Size: 399 639 111 411
Fragments arranged by size:
639 411 399 111
SacI G_AGCT'C
Cuts at: 0 290 1560
Size: 290 1270
SacII CC_GC'GG
Cuts at: 0 36 1560
Size: 36 1524
SalI G'TCGA_C
Cuts at: 0 1519 1560
Size: 1519 41
SanDI GG'GwC_CC
Cuts at: 0 1045 1560
Size: 1045 515
SfoI GGC'GCC
Cuts at: 0 1506 1560
Size: 1506 54
SmlI C'TyrA_G
Cuts at: 0 383 1255 1560
Size: 383 872 305
Fragments arranged by size:
872 383 305
SspI AAT'ATT
Cuts at: 0 253 1560
Size: 253 1307
StuI AGG'CCT
Cuts at: 0 1426 1560
Size: 1426 134
StyI C'CwwG_G
Cuts at: 0 187 905 1427 1560
Size: 187 718 522 133
Fragments arranged by size:
718 522 187 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 702 1147 1560
Size: 702 445 413
Fragments arranged by size:
702 445 413
TatI w'GTAC_w
Cuts at: 0 592 995 1560
Size: 592 403 565
Fragments arranged by size:
592 565 403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 154 186 1560
Size: 154 32 1374
Fragments arranged by size:
1374 154 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1305 1560
Size: 1305 255
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaI BanI
BanII BbeI BbsI BbvCI BcgI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BstAPI BstEII BstYI BtgI BtsI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KasI MfeI MmeI MscI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PpuMI
PshAI PspOMI PvuII SacI SacII SalI SanDI SfoI
SmlI SspI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BciVI BclI BfrBI BglII BlpI BmtI
BsaI BsaAI BsaBI BsaWI BseYI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MluI MslI
NdeI NheI NotI NruI NsiI PacI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PstI PvuI RsrII
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI