################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MslI MspA1I MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 314 GCGG CCGC(-3/-1) AflII 45 CTTAAG C^TTAAG BamHI 126 GGATCC G^GATCC BanII 281 GGGCTC G[GA]GC[CT]^C BbvCI 181 GCTGAGG CCTCAGC(-5/-2) BcnI 579 CCCGG CC^[GC]GG BfaI 530 CTAG C^TAG BfrI 45 CTTAAG C^TTAAG BmyI 281 GGGCTC G[AGT]GC[ACT]^C BpmI 553 CTGGAG CTGGAG(16/14) Bsp1286I 281 GGGCTC G[AGT]GC[ACT]^C BstF5I 173 CATCC GGATG(2/0) BstYI 126 GGATCC [GA]^GATC[CT] Cac8I 133 GCAAGC GC[ACGT]^[ACGT]GC EarI 595 GAAGAG CTCTTC(1/4) FokI 173 CATCC GGATG(9/13) HapII 580 CCGG C^CGG HinfI 265 GAATC G^A[ACGT]TC HpaII 580 CCGG C^CGG HphI 342 TCACC GGTGA(8/7) Ksp632I 595 GAAGAG CTCTTC(1/4) MaeI 530 CTAG C^TAG MaeII 246 ACGT A^CGT MfeI 218 CAATTG C^AATTG MflI 126 GGATCC [GA]^GATC[CT] MspI 580 CCGG C^CGG MunI 218 CAATTG C^AATTG NciI 579 CCCGG CC^[GC]GG NlaIV 126 GGATCC GG[ACGT]^[ACGT]CC SfaNI 190 GATGC GCATC(5/9) SfcI 251 CTGTAG C^T[GA][CT]AG SmlI 45 CTTAAG C^T[CT][GA]AG TfiI 265 GAATC G^A[AT]TC XbaI 529 TCTAGA T^CTAGA XcmI 486 CCATACCATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 126 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BbvI 193 GCTGC GCAGC(8/12) 202 GCTGC DraII 548 AGGACCT [GA]G^G[ACGT]CC[CT] 617 AGGACCT DsaI 176 CCATGG C^C[GA][CT]GG 197 CCATGG EcoO109I 548 AGGACCT [GA]G^G[ACGT]CC[CT] 617 AGGACCT EcoT14I 176 CCATGG C^C[AT][AT]GG 197 CCATGG Fnu4HI 193 GCTGC GC^[ACGT]GC 202 GCTGC HindIII 48 AAGCTT A^AGCTT 100 AAGCTT ItaI 193 GCTGC GC^[ACGT]GC 202 GCTGC MseI 46 TTAA T^TAA 320 TTAA MwoI 193 GCTGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 196 GCCATGGCTGC NcoI 176 CCATGG C^CATGG 197 CCATGG PpuMI 548 AGGACCT [GA]G^G[AT]CC[CT] 617 AGGACCT StyI 176 CCATGG C^C[AT][AT]GG 197 CCATGG Tru9I 46 TTAA T^TAA 320 TTAA TseI 193 GCTGC G^C[AT]GC 202 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI AlwI AvaII BsaJI BseRI BsiYI BslI BssKI BstNI Cfr13I DdeI DpnII EcoRII MaeIII MboI MboII MnlI MvaI NdeII NlaIII Sau3AI Sau96I ScrFI Tsp509I